| efficient replication of rhesus cytomegalovirus variants in multiple rhesus and human cell types. | rhesus cytomegalovirus infection of rhesus macaques has emerged as a model for human cytomegalovirus pathogenesis. the ul128-ul131 locus of the human virus is a primary determinant for viral entry into epithelial cells, an important cell type during cytomegalovirus infection. rhesus cytomegalovirus strain 68-1 spreads slowly when grown in cultured rhesus epithelial cells, and it does not code for orfs corresponding to ul128 and the second exon of ul130. we repaired the ul128-ul131 locus of strai ... | 2008 | 19064925 |
| coding potential of ul/b' from the initial source of rhesus cytomegalovirus strain 68-1. | rhesus cytomegalovirus (rhcmv) 68-1 is the prototypic strain of rhcmv that has been used for pathogenesis and vaccine development. we determined the complete sequence of the rhcmv 68-1 ul/b' region directly from the original urine from which rhcmv 68-1 was isolated in 1968, and compared it to other rhcmvs. the laboratory passaged rhcmv 68-1 has inversions, deletions, and stop codons in ul/b' that are absent in the original isolate and other low passage rhcmv isolates. fourteen of the 17 open rea ... | 2013 | 24210116 |
| reevaluation of the coding potential and proteomic analysis of the bac-derived rhesus cytomegalovirus strain 68-1. | cytomegaloviruses are highly host restricted, resulting in cospeciation with their hosts. as a natural pathogen of rhesus macaques (rm), rhesus cytomegalovirus (rhcmv) has therefore emerged as a highly relevant experimental model for pathogenesis and vaccine development due to its close evolutionary relationship to human cmv (hcmv). most in vivo experiments performed with rhcmv employed strain 68-1 cloned as a bacterial artificial chromosome (bac). however, the complete genome sequence of the 68 ... | 2012 | 22718821 |