| identification and sequence analysis of potato yellow vein virus capsid protein minor gene. | potato yellow vein virus (pyvv) is a whitefly-transmitted (trialeurodes vaporariorum) closterovirus (wtc) with an as yet unidentified genome composition. pyvv dsrna preparations consist of three high molecular weight dsrna species (dsrnas 1, 2 and 3) 8.0, 5.5 and 4.0 kbp in size respectively, as well as two low molecular weight dsrna species of 2.0 and 1.8 kbp (denoted x and y). the pyvv capsid protein minor (cpm) gene was identified on the dsrna 3 species, and was subsequently cloned and sequen ... | 2002 | 12881642 |
| molecular variation of potato yellow vein virus isolates. | to evaluate the variation of potato yellow vein virus from potato fields, 12 isolates were collected from colombia and one was collected from peru. double-stranded rna was extracted from the plants and used as a template for rt-pcr amplification of the coat protein ( cp) gene and, in separate reactions the c-terminal region of the heat shock protein 70 homologue ( hsp70h) gene and the n-terminal region of the p60 open reading frame. the cp amplicons were subjected to single-strand conformation p ... | 2004 | 15045568 |
| analysis of the rna of potato yellow vein virus: evidence for a tripartite genome and conserved 3'-terminal structures among members of the genus crinivirus. | double-stranded rna preparations produced from potato plants graft-inoculated with a peruvian isolate of potato yellow vein virus (pyvv; genus crinivirus, family closteroviridae) contain five rna species denoted rna 1, rna 2, rna 3, x and y of approximately 8, 5.3, 3.8, 2.0 and 1.8 kbp, respectively. the complete nucleotide sequences of pyvv rnas 1, 2 and 3 and northern hybridization analysis showed that pyvv rna 1 contained the replication module and an additional open reading frame (p7), while ... | 2004 | 15218192 |
| sequences of defective rnas associated with potato yellow vein virus. | | 2006 | 16155728 |
| development of real-time and conventional rt-pcr assays for the detection of potato yellow vein virus (pyvv). | potato yellow vein virus (pyvv) is considered a quarantine pathogen in the european and mediterranean plant protection organization (eppo) area. this virus is widespread and damaging at its centre of origin in south america. current detection methods are either time-consuming or difficult to interpret. this paper reports the development of a sensitive, high throughput, real-time reverse transcription (rt)-pcr assay, based on taqman chemistry, suitable for pyvv detection. in addition, a reliable ... | 2006 | 16712962 |