| bacteremia caused by a novel bordetella species, "b. hinzii". | bordetella spp. cause respiratory tract diseases in warm-blooded animals. only bordetella bronchiseptica has been reported to cause bacteremia in humans, and this rare infection usually occurs with pneumonia in immunocompromised patients. we describe "bordetella hinzii" bacteremia in an aids patient without a respiratory illness. combining biochemical phenotyping with fatty acid analysis permitted preliminary identification of this previously undescribed pathogen; identity was confirmed by dna-d ... | 1994 | 7814500 |
| bordetella hinzii sp. nov., isolated from poultry and humans. | a polyphasic taxonomic study that included dna-rrna hybridizations, dna-dna hybridizations, dna base ratio determinations, whole-cell protein and fatty acid analyses, and an examination of classical phenotypic characteristics was performed in order to classify human and veterinary isolates that resemble bordetella avium. twelve poultry isolates and two human isolates were assigned to a new species, for which we propose the name bordetella hinzii. the position of this organism in the family alcal ... | 1995 | 7857806 |
| characteristics of bordetella hinzii strains isolated from a cystic fibrosis patient over a 3-year period. | over a 3-year period, an adult cystic fibrosis patient underwent eight episodes of pulmonary exacerbation of his disease. at least one of two different strains of bordetella hinzii could be isolated from sputum samples in every instance. the differentiation of b. hinzii from related taxa and its role as an etiologic agent of infections are discussed. the two isolates of b. hinzii reported are the third and fourth human-derived strains described in the literature. | 1996 | 8815118 |
| application of 16s rrna gene sequencing to identify bordetella hinzii as the causative agent of fatal septicemia. | we report on the first case of fatal septicemia caused by bordetella hinzii. the causative organism exhibited a biochemical profile identical to that of bordetella avium with three commercial identification systems (api 20e, api 20 ne, and vitek gni+ card). however, its cellular fatty acid profile was not typical for either b. avium or previously reported strains of b. hinzii. presumptive identification of the patient's isolate was accomplished by traditional biochemical testing, and definitive ... | 2000 | 10655386 |
| restriction enzyme analysis and ribotyping distinguish bordetella avium and bordetella hinzii isolates. | fifty-seven bacterial isolates previously identified as bordetella avium or b. hinzii were characterized by restriction enzyme analysis (rea) and/or ribotyping. twenty restriction endonucleases were evaluated for rea. digestion of chromosomal dna from the 42 b. avium and 15 b. hinzii isolates with hinfi produced 8 and 7 distinct fingerprint profiles, respectively. digestion with ddei further discriminated these bordetella species and produced 12 fingerprint profiles for b. avium and 4 profiles o ... | 2000 | 10722134 |
| structure of the lipid a of bordetella hinzii atcc 51730. | bordetella hinzii has recently been isolated from immunocompromised human hosts. the structure of the lipid a of its endotoxin was investigated using chemical analyses, nuclear magnetic resonnance (nmr), gas liquid chromatography/mass spectrometry (gc/ms), plasma desorption mass spectrometry (pdms) and matrix-assisted laser desorption/ionization (maldi) mass spectrometry. the lipid a contains the classical bisphosphorylated beta-(1-->6)-linked d-glucosamine disaccharide with hydroxytetradecanoic ... | 2000 | 10775094 |
| the structure of the carbohydrate backbone of the lipopolysaccharides from bordetella hinzii and bordetella bronchiseptica. | the structure of the core-lipid a region of the lipopolysaccharides from bordetella hinzii and bordetella bronchiseptica has been analyzed. lipopolysaccharides were deacylated using strong alkaline hydrolysis, the products were separated by high performance anion-exchange chromatography and analyzed by nmr and mass spectrometry. the following structure of the products can be deduced from the experimental results: where for the product from bordetella hinzii n = h, r = h, beta-fucn4n- or partiall ... | 2000 | 10880983 |
| bordetella hinzii, a "new" opportunistic pathogen to think about. | | 2000 | 10908033 |
| discovery, purification, and characterization of a temperate transducing bacteriophage for bordetella avium. | we discovered and characterized a temperate transducing bacteriophage (ba1) for the avian respiratory pathogen bordetella avium. ba1 was initially identified along with one other phage (ba2) following screening of four strains of b. avium for lysogeny. of the two phage, only ba1 showed the ability to transduce via an allelic replacement mechanism and was studied further. with regard to host range, ba1 grew on six of nine clinical isolates of b. avium but failed to grow on any tested strains of b ... | 2000 | 11029434 |
| chemical and serological characterization of the bordetella hinzii lipopolysaccharides. | bordetella hinzii has recently been isolated from immunocompromised human hosts. the polysaccharides isolated from its endotoxin (lipopolysaccharide, lps) were investigated using chemical analyses, nmr, gas-liquid chromatography/mass spectrometry and mass spectrometry by plasma desorption, matrix-assisted laser desorption/ionization and electrospray. the following structure for the o-chain-free lps was deduced from the experimental results: carbohydrate structure [see text] mass spectrometry and ... | 2000 | 11086162 |
| structure of the o-specific polysaccharide chain of the lipopolysaccharide of bordetella hinzii. | the lipopolysaccharide of bordetella hinzii was analyzed after various chemical degradations by nmr spectroscopy and maldi mass spectrometry, and the following structure of the polysaccharide chain was determined: 4-o-me-alpha-galpnac3nacan-(1-->[-->4)-beta-glcpnac3nacan-(1-->4)-beta-glcpnac3nacan-(1-->4)-alpha-galpnac3nacan-(1-](n)-where glcnac3nacan and galnac3nacan stand for 2,3-diacetamido-2,3-dideoxy-glucuronamide and -galacturonamide, respectively. the polysaccharide chain is terminated wi ... | 2002 | 12007480 |
| characterization of unusual bacteria isolated from respiratory secretions of cystic fibrosis patients and description of inquilinus limosus gen. nov., sp. nov. | using a polyphasic approach (including cellular protein and fatty acid analysis, biochemical characterization, 16s ribosomal dna sequencing, and dna-dna hybridizations), we characterized 51 bacterial isolates recovered from respiratory secretions of cystic fibrosis (cf) patients. our analyses showed that 24 isolates belong to taxa that have so far not (or only rarely) been reported from cf patients. these taxa include acinetobacter sp., bordetella hinzii, burkholderia fungorum, comamonas testost ... | 2002 | 12037065 |
| comparison of ribotyping and restriction enzyme analysis for inter- and intraspecies discrimination of bordetella avium and bordetella hinzii. | bordetella avium is an avian respiratory disease pathogen responsible for substantial economic losses to the turkey industry. the inability to distinguish isolates has hampered outbreak investigations and prevents a complete understanding of transmission mechanisms. isolates of bordetella hinzii, often referred to as b. avium-like or as alcaligenes faecalis type ii prior to 1995, have also been acquired from the respiratory tracts of diseased poultry but are not believed to be pathogenic for bir ... | 2003 | 12682138 |
| chronic cholangitis caused by bordetella hinzii in a liver transplant recipient. | bordetella hinzii was isolated in four biliary specimens collected over 6 months from a liver transplant recipient with cholangitis. the isolates were resistant to most beta-lactam antibiotics and fluoroquinolones. molecular typing was performed by pulsed-field gel electrophoresis. these data add cholangitis to the spectrum of disease manifestations caused by b. hinzii. | 2004 | 15131227 |
| functional characterization of the bvgas two-component system of bordetella holmesii. | the bvgas two-component system is the master regulator of virulence gene expression in the mammalian pathogens bordetella pertussis, bordetella parapertussis and bordetella bronchiseptica. this paper reports the partial cloning and characterization of the bvgas loci of the 'new' bordetella species bordetella holmesii, bordetella trematum and bordetella hinzii, which are increasingly recognized as opportunistic pathogens in humans. it is demonstrated that the cytoplasmic signalling domains of the ... | 2004 | 15528658 |
| adenylate cyclase toxin (act) from bordetella hinzii: characterization and differences from act of bordetella pertussis. | bordetella hinzii is a commensal respiratory microorganism in poultry but is increasingly being recognized as an opportunistic pathogen in immunocompromised humans. although associated with a variety of disease states, practically nothing is known about the mechanisms employed by this bacterium. in this study, we show by dna sequencing and reverse transcription-pcr that both commensal and clinical strains of b. hinzii possess and transcriptionally express cyaa, the gene encoding adenylate cyclas ... | 2005 | 16267282 |
| evaluation of pyrrolidonyl arylamidase for the identification of nonfermenting gram-negative rods. | to evaluate the activity of pyrrolidonyl arylamidase (pyr) for the differentiation and identification of nonfermenting gram negative rods (nfgnr), 293 isolates were tested. a 24 h culture of each test organism was prepared. from this a 108-109 cfu/ml suspension was added to 0.25 ml of sterile physiologic solution. a pyr disk was then added and the test was incubated for 30 minutes at 35-37 degrees c, at environmental atmosphere. reading was done by adding 1 drop of cinnamaldehyde reagent. strain ... | 2007 | 16822636 |
| the structure of the core-o-chain linkage region of the lipopolysaccharide from bordetella hinzii. | linkage region between core and the o-chain of the lipopolysaccharide from bordetella hinzii has been analyzed by nmr and ms analysis of the products, obtained by anhydrous hf treatment or consecutive ammonia and acoh treatment of the lps. the following structure of this region was deduced from the experimental results: [structure: see text] this structure is identical to the structure of the respective region of bordetella parapertussis lps. polysaccharide part (ps) consists of not more than 15 ... | 2007 | 17123490 |
| a uk clinical isolate of bordetella hinzii from a patient with myelodysplastic syndrome. | what is believed to be the first clinical isolate of bordetella hinzii in the uk, from a patient with myelodysplastic syndrome, is described. this patient had no known avian exposure, and the source of the organism remains unknown. it appears that the underlying immune deficiency of the patient increased the susceptibility to opportunistic infection with this organism. human infection with b. hinzii is rare and this species is difficult to differentiate from bordetella avium by routine phenotypi ... | 2007 | 18033844 |
| identification of bordetella spp. in respiratory specimens from individuals with cystic fibrosis. | bordetella spp. are not normally included when considering the opportunistic bacterial species that are typically involved in respiratory tract infections in individuals with cystic fibrosis (cf). by using a combination of bacterial genotyping and 16s rdna sequencing, bordetella spp. were identified in cultures obtained from 43 individuals with cf. most (n = 23) patients were infected with bordetella bronchiseptica/parapertussis; five were infected with bordetella hinzii, four with bordetella pe ... | 2008 | 18325036 |
| bordetella hinzii septicemia in association with epstein-barr virus viremia and an epstein-barr virus-associated diffuse large b-cell lymphoma. | we report the 1st case of bordetella hinzii septicemia associated with epstein-barr virus viremia and lymphoma. b. hinzii identification necessitated cellular fatty acid analysis by gas-liquid chromatography and 16s rrna gene sequencing. isolates were resistant to many antimicrobials. resistance and diagnostic challenges complicated management and contributed to mortality. | 2008 | 18482816 |
| matrix-assisted laser desorption ionization-time of flight mass spectrometry for identification of nonfermenting gram-negative bacilli isolated from cystic fibrosis patients. | the identification of nonfermenting gram-negative bacilli isolated from cystic fibrosis (cf) patients is usually achieved by using phenotype-based techniques and eventually molecular tools. these techniques remain time-consuming, expensive, and technically demanding. we used a method based on matrix-assisted laser desorption ionization-time of flight mass spectrometry (maldi-tof-ms) for the identification of these bacteria. a set of reference strains belonging to 58 species of clinically relevan ... | 2008 | 18685005 |
| study of a bordetella hinzii isolate from a laboratory mouse. | bordetella hinzii isolated from the trachea and lungs of a laboratory mouse with a respiratory infection was identified based on its phenotypic and genetic traits. the mouse showed sneezing with a chattering sound but without nasal discharge, and histopathologic examination revealed rhinitis, tracheitis, and bronchopneumonia. the isolate was a gram-negative, oxidase- and catalase-positive, short rod-shaped organism that produced alkali from malonate. the results of biochemical identification, an ... | 2008 | 19004369 |
| strain-specific virulence of bordetella hinzii in poultry. | bordetella hinzii is commonly acquired from the respiratory tract of diseased poultry but is generally regarded as nonpathogenic in avian hosts because attempts to demonstrate disease following experimental infection of chickens and turkeys have failed. recently, with the availability of highly specific dna-based methods for identification of this agent, it was recognized that some isolates used in previous studies were misidentified at the time of their acquisition as bordetella avium, b. avium ... | 2009 | 19432003 |
| temporal bacterial diversity and detection of putative methanotrophs in surface mats of lonar crater lake. | the phylogenetic diversity of bacterial communities in microbial mats of two different seasons from saline and hyperalkaline lonar lake was investigated using 16s rrna gene library analysis. arthrospira (cyanobacteria) related clones (>80% of total clones) dominated libraries of both the seasons. clear differences were found in both the seasons as the operational taxonomic units (otus) related to fusibacter (lai-1 and lai-59) and tindallia magadiensis (lai-27) found in post-monsoon were not foun ... | 2010 | 20586073 |
| evaluation of specificity of bp3385 for bordetella pertussis detection. | bp3385 has been proposed as a diagnostic pcr target for discriminating between bordetella pertussis and other bordetella species that also infect humans. our results demonstrate that this gene is also present in some strains of bordetella hinzii and bordetella bronchiseptica. | 2010 | 20631116 |
| prevalence of bordetella hinzii in mice in experimental facilities in japan. | to reveal the current status of the prevalence of bordetella hinzii in mice in experimental facilities in japan, a survey of this agent was performed by culture of tracheal swabs from a total of 12,923 mice from 1699 facilities (12,192 mice from 1572 facilities in universities and research institutes and 731 mice from 127 facilities in pharmaceutical companies) in total. in the results, 195 out of 12,192 mice (1.6%) from 44 out of 1572 facilities (2.8%) in universities and research institutes we ... | 2011 | 22130558 |
| opportunistic pulmonary bordetella hinzii infection after avian exposure. | we report 2 cases of pulmonary bordetella hinzii infection in immunodeficient patients. one of these rare cases demonstrated the potential transmission of the bacteria from an avian reservoir through occupational exposure and its persistence in humans. we establish bacteriologic management of these infections and suggest therapeutic options if needed. | 2015 | 26584467 |
| draft genome sequences of bordetella hinzii and bordetella trematum. | bordetella hinzii colonizes the respiratory tracts of poultry but can also infect immunocompromised humans. bordetella trematum, however, only infects humans, causing ear and wound infections. here, we present the first draft genome sequences of strains b. hinzii atcc 51730 and b. trematum ccug 13902. | 2013 | 24158552 |
| survival of bacteria of laboratory animal origin on cage bedding and inactivation by hydrogen peroxide vapour. | this study aims to determine the ability of laboratory animal bacteria to resist desiccation and inactivation by hydrogen peroxide vapour (hpv) on paper bedding pieces. bedding pieces were saturated with bacterial suspensions in water or 2% (w/v) bovine serum albumin (bsa) in water, and held in a mouse facility. viable counts showed variable survival rates over time for the bacterial species used ([pasteurella] pneumotropica, muribacter muris, pseudomonas aeruginosa, acinetobacter redioresistens ... | 2016 | 27932683 |
| development of a pcr for identification of bordetella hinzii. | bordetella hinzii infects primarily poultry and immunocompromised humans. it is closely related to the etiologic agent of turkey coryza, bordetella avium. distinguishing between b. avium and b. hinzii is difficult, and there is no method for identification of b. hinzii suitable for use by diagnostic laboratories. this report details the development of a b. hinzii-specific pcr targeting the ompa gene. assay sensitivity is 100% based on analysis of 48 b. hinzii isolates from diverse geographic loc ... | 2013 | 24689192 |
| complete bordetella avium, bordetella hinzii and bordetella trematum lipid a structures and genomic sequence analyses of the loci involved in their modifications. | endotoxin is recognized as one of the virulence factors of the bordetella avium bird pathogen, and characterization of its structure and corresponding genomic features are important for an understanding of its role in pathogenicity and for an improved general knowledge of bordetella spp virulence factors. the structure of the biologically active part of b. avium lps, lipid a, is described and compared to those of another bird pathogen, opportunistic in humans, bordetella hinzii, and to that of b ... | 2014 | 24127384 |
| environmental origin of the genus bordetella. | members of the genus bordetella include human and animal pathogens that cause a variety of respiratory infections, including whooping cough in humans. despite the long known ability to switch between a within-animal and an extra-host lifestyle under laboratory growth conditions, no extra-host niches of pathogenic bordetella species have been defined. to better understand the distribution of bordetella species in the environment, we probed the ncbi nucleotide database with the 16s ribosomal rna ( ... | 2017 | 28174558 |
| draft genome of bordetella pseudohinzii bh370 isolated from trachea and lung tissues of a laboratory mouse. | in this study, we present the draft genome sequence of b. pseudohinzii bh370 recovered from the trachea and lung tissues of an icr mouse in malaysia. the genome consists of 4,474,040 bp with a gc content of 66.4%. annotation using rast algorithm displayed 5119 protein encoding and 52 rna genes. the crispr-cas genomic sequences previously reported in b. pseudohinzii were identified. the nucleotide sequences of bh370 was deposited into the european nucleotide archive under the genome assembly acce ... | 2017 | 28377885 |
| bordetella pseudohinzii as a confounding organism in murine models of pulmonary disease. | a group studying acute lung injury observed an increased percentage of neutrophils in the bronchoalveolar lavage (bal) fluid of mice. bal was performed, and lung samples were collected sterilely from 5 c57bl/6 mice that had been bred inhouse. pure colonies of bacteria, initially identified as bordetella hinzii were cultured from 2 of the 5 mice which had the highest percentages of neutrophils (21% and 26%) in the bal fluid. subsequent sequencing of a portion of the ompa gene from this isolate de ... | 2016 | 27780002 |
| identification and taxonomic characterization of bordetella pseudohinzii sp. nov. isolated from laboratory-raised mice. | bordetella hinzii is known to cause respiratory disease in poultry and has been associated with a variety of infections in immunocompromised humans. in addition, there are several reports of b. hinzii infections in laboratory-raised mice. here we sequenced and analysed the complete genome sequences of multiple b. hinzii-like isolates, obtained from vendor-supplied c57bl/6 mice in animal research facilities on different continents, and we determined their taxonomic relationship to other bordetell ... | 2016 | 27707434 |
| correction for weigand et al., complete genome sequences of two bordetella hinzii strains isolated from humans. | | 2016 | 26769949 |
| complete genome sequences of two bordetella hinzii strains isolated from humans. | bordetella hinzii is primarily recovered from poultry but can also colonize mammalian hosts and immunocompromised humans. here, we report the first complete genome sequences of b. hinzii in two isolates recovered from humans. the availability of these sequences will hopefully aid in identifying host-specific determinants variably present within this species. | 2015 | 26316634 |
| bordetella bronchialis sp. nov., bordetella flabilis sp. nov. and bordetella sputigena sp. nov., isolated from human respiratory specimens, and reclassification of achromobacter sediminum zhang et al. 2014 as verticia sediminum gen. nov., comb. nov. | the phenotypic and genotypic characteristics of four bordetella hinzii-like strains from human respiratory specimens and representing nrda gene sequence based genogroups 3, 14 and 15 were examined. in a 16s rrna gene sequence based phylogenetic tree, the four strains consistently formed a single coherent lineage but their assignment to the genus bordetella was equivocal. the respiratory quinone, polar lipid and fatty acid profiles generally conformed to those of species of the genus bordetella a ... | 2015 | 26220296 |
| draft genome sequences of six bordetella hinzii isolates acquired from avian and mammalian hosts. | bordetella hinzii is a gram-negative bacterium known to infect poultry, humans, rabbits, and rodents. it is an opportunistic pathogen in immunocompromised humans, and some strains cause mild to moderate respiratory disease in turkeys. little is known as to the degree of genetic diversity within the species or the genetic basis for virulence. here, we report the genome sequences of six isolates of b. hinzii acquired from humans, rabbits, or turkeys. these data provide a framework for refining the ... | 2015 | 25792043 |
| 16s ribosomal dna sequence-based identification of bacteria in laboratory rodents: a practical approach in laboratory animal bacteriology diagnostics. | correct identification of bacteria is crucial for the management of rodent colonies. some bacteria are difficult to identify phenotypically outside reference laboratories. in this study, we evaluated the utility of 16s ribosomal dna (rdna) sequencing as a means of identifying a collection of 30 isolates of rodent origin which are conventionally difficult to identify. sequence analysis of the first approximate 720 to 880 bp of the 5'- end of 16s rdna identified 25 isolates (83.33%) with ≥ 99% sim ... | 2014 | 24876090 |
| respiratory infection caused by bordetella hinzii. | | 2013 | 23755859 |
| bordetella hinzii in rodents, southeast asia. | | 2013 | 23750354 |