| sequence and mutational analysis of the common nodbcij region of rhizobium sp. (oxytropis arctobia) strain n33, a nitrogen-fixing microsymbiont of both arctic and temperate legumes. | by heterologous hybridization, we have identified the common nodulation genes nodbcij of rhizobium sp. strain n33 within a 8.2-kb psti fragment. the nodbcij genes are located within a 4,620-bp region which also included a consensus nod box promoter. the four open reading frames coding for the nodbcij genes contain 657, 1,353, 915, and 789 nucleotides, respectively. we found that the noda gene was not adjacent to the nodb gene, unlike the situation in many rhizobia. the dna of the nodbcij genes o ... | 1996 | 8755627 |
| characterization and mutational analysis of nodhpq genes of rhizobium sp. strain n33. | we have shown, by sequencing the nodulation gene region of rhizobium sp. strain n33 previously isolated from the canadian high arctic, that the nodhpq genes are located in a 4.8-kb region downstream of nodbcij. the open reading frames of nodhpq are 747, 906, and 1941 nucleotides long, respectively. the strain n33 genome contains one copy of nodh and two copies of nodpq that are homologous to those genes in rhizobium meliloti. tn5 insertions in the nodhpq genes of strain n33 did not affect the fo ... | 1996 | 8870271 |
| sequence and mutational analysis of the 6.7-kb region containing nodafeg genes of rhizobium sp. strain n33: evidence of dna rearrangements. | a 6.7-kb region upstream of nodbc genes in rhizobium sp. strain n33 was shown to contain the nodafeg genes and an open reading frame designated orfz. the open reading frames for these genes contain 591, 282, 1209, 738, and 1,338 nucleotides respectively. homologues of these genes were found in other rhizobia with the exception of orfz, for which there was no counterpart found in the genbank/embl database. tn5 mutagenesis in nodeg and in the intergenic nodg-b region has shown a nod+ phenotype on ... | 1997 | 9100384 |