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comparative analysis of pseudomonas syringae pv. actinidiae and pv. phaseolicola based on phaseolotoxin-resistant ornithine carbamoyltransferase gene (argk) and 16s-23s rrna intergenic spacer sequences.pseudomonas syringae pv. phaseolicola, which causes halo blight on various legumes, and pv. actinidiae, responsible for canker or leaf spot on actinidia plants, are known as phaseolotoxin producers, and the former possesses phaseolotoxin-resistant ornithine carbamoyltransferase (roct) which confers resistance to the toxin. we confirmed that the latter is also resistant to phaseolotoxin and possesses roct, and we compared the two pathovars by using sequence data of the roct gene and the intergeni ...19978979356
[prevalent forecast of kiwifruit bacterial canker caused by pseudomonas syringae pv. actinidiae].the prevalent analysis of kiwifruit bacterial canker for several years showed that the effective ecological factors of severe degree were the precipitation (x1) in the second and last ten-days of march, and the average temperature (x2) of january. the model was y = 2.1359 + 0.0107x1 - 0.6061x2. the main factor of the prevalence was the relative variation of ten-days average temperature and precipitation in winter and in early spring, and the regression equation was y = -8.127 + 22.739x - 13.254x ...200111758412
dna markers for identification of pseudomonas syringae pv. actinidiae.the specific dna fragment was screened by rapd analysis of pseudomonas syringae pv. actinidiae, as well as similar strains that were isolated from kiwifruits. the primer c24 detected a fragment that is specific in p. syringae pv. actinidiae. this fragment was cloned. the pathovar-specific fragment was detected from a southern blot analysis of the genomic dnas of p. syringae pv. actinidiae using the cloned fragment as a probe. the sequence size of the cloned fragment was determined as 675 bp. a d ...200212018854
clonal population structure of pseudomonas avellanae strains of different origin based on multilocus enzyme electrophoresis.to assess the genetic diversity and genetic relationships of pseudomonas avellanae, the causative agent of hazelnut decline, a total of 102 strains, obtained from central italy (provinces of viterbo and rome) and northern greece, were studied using multilocus enzyme electrophoresis (mlee). their allelic variation in 10 loci was determined. all loci were polymorphic and 53 electrophoretic types (ets) were identified from the total sample. the mean genetic diversity (h) was 0.65 and this value ran ...200314523121
occurrence of the stra-strb streptomycin resistance genes in pseudomonas species isolated from kiwifruit plants.the occurrence of stra-strb streptomycin-resistance genes within transposon tn5393 was examined in pseudomonas syringae pv. actinidiae, p. syringae pv. syringae, and p. marginalis, isolated from kiwifruit plants in korea and japan. pcr amplification with primers specific to stra-strb revealed that three of the tested pseudomonas species harbored these genes for a streptomycin-resistance determinant. tn5393, containing stra-strb, was also identified with pcr primers designed to amplify parts of t ...200415650697
Pseudomonas syringae pv. actinidiae Draft Genomes Comparison Reveal Strain-Specific Features Involved in Adaptation and Virulence to Actinidia Species.A recent re-emerging bacterial canker disease incited by Pseudomonas syringae pv. actinidiae (Psa) is causing severe economic losses to Actinidia chinensis and A. deliciosa cultivations in southern Europe, New Zealand, Chile and South Korea. Little is known about the genetic features of this pathovar. We generated genome-wide Illumina sequence data from two Psa strains causing outbreaks of bacterial canker on the A. deliciosa cv. Hayward in Japan (J-Psa, type-strain of the pathovar) and in Italy ...201122132095
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