| grapevine fleck virus-like viruses in vitis. | two sets of degenerate primers for the specific amplification of 572-575 nt and 386 nt segments of the methyltransferase and rna- dependent rna polymerase cistrons of members of the genera tymovirus and marafivirus and of the unassigned virus grapevine fleck virus (gfkv) were designed on the basis of available sequences. these primers were used for amplifying and subsequent cloning and sequencing part of the open reading frame 1 of the genome of gfkv, grapevine asteroid mosaic-associated virus ( ... | 2000 | 10795522 |
| the complete nucleotide sequence and genomic characterization of grapevine asteroid mosaic associated virus. | in analyzing grapevine clones infected with grapevine red blotch associated virus, we identified a small number of isometric particles of approximately 30nm in diameter from an enriched fraction of leaf extract. a dominant protein of 25kda was isolated from this fraction using sds-page and was identified by mass spectrometry as belonging to grapevine asteroid mosaic associated virus (gamav). using a combination of three methods rna-seq, srna-seq, and sanger sequencing of rt- and race-pcr product ... | 2017 | 27720957 |
| sequence analysis of the 3' end of three grapevine fleck virus-like viruses from grapevine. | the 3' end of the genome of three grapevine fleck virus-like viruses, i.e. grapevine redglobe virus (grgv), grapevine asteroid mosaic-associated virus (gamav), and an unidentified virus from a greek grapevine (accession gr8-19) was amplified from reverse transcribed total nucleic acid extracts from infected grapevine tissues and sequenced. the analysed genome portions differed in size and organization. the 3' ends of gamav (1852 nt) and of gr8-19 (1791 nt) resembled that of marafiviruses, as bot ... | 2003 | 12913354 |