differential expression of chitinases in vitis vinifera l. responding to systemic acquired resistance activators or fungal challenge. | the concept of systemic acquired resistance (sar) enables a novel approach to crop protection, and particular pathogenesis-related proteins, i.e. an acidic chitinase, have been classified as markers of the sar response. basic class i (vchit1b) and a class iii (vch3) chitinase cdnas were cloned from cultured vitis vinifera l. cv pinot noir cells and used to probe the induction response of grapevine cells to salicylic acid or yeast elicitor. furthermore, the cells were treated with the commercial ... | 1997 | 9390436 |
analysis of transgenic grapevine (vitis rupestris) and nicotiana benthamiana plants expressing an arabis mosaic virus coat protein gene. | a disarmed lba4404 strain of agrobacterium tumefaciens harboring a binary vector which contained chimeric genes encoding the neomycin phosphotransferase (npt ii) and the coat protein (cp) of arabis mosaic nepovirus (armv) was used in co-cultivation experiments with leaf discs of nicotiana benthamiana and somatic embryos of the grapevine rootstock cultivar vitis rupestris. transgenic n. benthamiana expressing the armv cp gene were regenerated and six independent lines were characterized. enzyme-l ... | 2000 | 10936531 |
a genetic linkage map of grape, utilizing vitis rupestris and vitis arizonica. | a genetic linkage map of grape was constructed, utilizing 116 progeny derived from a cross of two vitis rupestris x v. arizonica interspecific hybrids, using the pseudo-testcross strategy. a total of 475 dna markers-410 amplified fragment length polymorphism, 24 inter-simple sequence repeat, 32 random amplified polymorphic dna, and nine simple sequence repeat markers-were used to construct the parental maps. markers segregating 1:1 were used to construct parental framework maps with confidence l ... | 2004 | 15292989 |
complete genome sequences of two new variants of grapevine rupestris stem pitting-associated virus and comparative analyses. | grapevine rupestris stem pitting-associated virus (grspav), a member of the genus foveavirus within the family flexiviridae, is the putative causal agent of the disease rupestris stem pitting (rsp) of grapevines. grspav comprises a family of variants whose pathological characteristics have not been determined. recently, many of the indicator "st george" plants (vitis rupestris) used throughout the world to index rsp tested positive for grspav. this finding questions the validity of past biologic ... | 2005 | 15831969 |
inheritance of resistance to xylella fastidiosa within a vitis rupestris x vitis arizonica hybrid population. | the inheritance of resistance to xylella fastidiosa (xf), the bacterium which causes pierce's disease (pd) in grapevines, was evaluated within a factorial mating design consisting of 16 full-sib families with resistance derived from vitis arizonica interspecific hybrids. measurements of disease progression under greenhouse conditions were based on quantitative assessment of xf populations in stem tissues and on three phenotypic scores: leaf scorch, a cane maturation index (cmi) and an index that ... | 2005 | 15864525 |
detection of xylella fastidiosa from resistant and susceptible grapevine by tissue sectioning and membrane entrapment immunofluorescence. | immunofluorescence detection was performed by tissue sectioning and membrane entrapment of xylella fastidiosa from the inoculated hybrid selection f8909-08 (vitis rupestris a. de serres x v. arizonica/candicans b43-17; resistant) and chardonnay (susceptible). in both techniques, tissue sections and bacteria-trapped polycarbonate membranes were incubated with specific polyclonal igg and stained with fluorescein isothiocyanate (fitc)-conjugated igg from rabbits to x. fastidiosa cells. the stained ... | 2005 | 16035233 |
refined mapping of the pierce's disease resistance locus, pdr1, and sex on an extended genetic map of vitis rupestris x v. arizonica. | a framework genetic map based on genomic dna-derived ssr, est-derived ssr, est-sts and est-rflp markers was developed using 181 genotypes generated from d8909-15 (female) x f8909-17 (male), the '9621' population. both parents are half siblings with a common female parent, vitis rupestris 'a. de serres', and different male parents (forms of v. arizonica). a total of 542 markers were tested, and 237 of them were polymorphic for the female and male parents. the female map was developed with 159 map ... | 2006 | 16960717 |
host range of a population of pratylenchus vulnus in commercial fruit, nut, citrus, and grape rootstocks in spain. | in a host-range study carried out under greenhouse conditions, a total of 37 commercial fruit tree, grape, and citrus rootstocks were tested for their reaction to a population of the lesion nematode, pratylenchus vulnus, in spain. twenty-five rootstocks had a pf/pi > 1.5. these included almond (desmayo rojo, 1143), apple (em-9, em-106), avocado (hass), cherry (santa lucia 64, camil, m x m 14, masto de montafiana), grape (41-b, fercal, ritcher 110), hazelnut (pauetet), loquat (nadal), peach (mont ... | 1992 | 19283047 |
resistance to crown gall disease in transgenic grapevine rootstocks containing truncated vire2 of agrobacterium. | a truncated form of the ti-plasmid vire2 gene from agrobacterium tumefaciens strains c58 and a6, and a. vitis strain cg450 was transferred and expressed in somatic embryos of grapevine rootstocks 110 richter (vitis rupestris × v. berlandieri), 3309 couderc (v. rupestris × v. riparia) and teleki 5c (v. berlandieri × v. riparia) via agrobacterium-mediated transformation to confer resistance to crown gall disease. transformation was confirmed in 98% of the 322 lines by enzyme-linked immunosorbent a ... | 2010 | 20182792 |
transmission competency of single-female xiphinema index lines for grapevine fanleaf virus. | grapevine fanleaf virus (gflv) is vectored specifically from grapevine to grapevine by the ectoparasitic nematode xiphinema index. limited information is available on the vector competency of x. index populations from diverse geographical origins. we determined the transmissibility of two gflv strains showing 4.6% amino acid divergence within their coat protein (e.g., strains f13 and ghu) by seven clonal lines of x. index developed from seven distinct populations from the mediterranean basin (cy ... | 2010 | 20205542 |
the phytoalexin resveratrol regulates the initiation of hypersensitive cell death in vitis cell. | resveratrol is a major phytoalexin produced by plants in response to various stresses and promotes disease resistance. the resistance of north american grapevine vitis rupestris is correlated with a hypersensitive reaction (hr), while susceptible european vitis vinifera cv. 'pinot noir' does not exhibit hr, but expresses basal defence. we have shown previously that in cell lines derived from the two vitis species, the bacterial effector harpin induced a rapid and sensitive accumulation of stilbe ... | 2011 | 22053190 |
defence signalling triggered by flg22 and harpin is integrated into a different stilbene output in vitis cells. | plants can activate defence to pathogen attack by two layers of innate immunity: basal immunity triggered by pathogen-associated molecular pattern (pamp) triggered immunity (pti) and effector-triggered immunity (eti) linked with programmed cell death. flg22 and harpin are evolutionary distinct bacterial pamps. we have previously shown that harpin triggers hypersensitive cell death mimicking eti in vitis rupestris, but not in the vitis vinifera cultivar 'pinot noir'. in contrast, the bacterial pa ... | 2012 | 22792328 |
transformation of grapevine rootstocks with the coat protein gene of grapevine fanleaf nepovirus. | control of fanleaf disease induced by the grapevine fanleaf nepovirus (gflv) today is based on sanitary selection and soil disinfection with nematicides. this way of control is not always efficient and nematicides can be dangerous pollutants. coat protein (cp) mediated protection could be an attractive alternative. we have transferred a chimeric cp gene of gflv-f13 via agrobacterium tumefaciens lba4404 into two rootstock varieties: vitis rupestris and 110 richter (v. rupestris x v. berlandieri). ... | 1995 | 24185595 |
water uptake along the length of grapevine fine roots: developmental anatomy, tissue-specific aquaporin expression, and pathways of water transport. | to better understand water uptake patterns in root systems of woody perennial crops, we detailed the developmental anatomy and hydraulic physiology along the length of grapevine (vitis berlandieri × vitis rupestris) fine roots from the tip to secondary growth zones. our characterization included the localization of suberized structures and aquaporin gene expression and the determination of hydraulic conductivity (lpr) and aquaporin protein activity (via chemical inhibition) in different root zon ... | 2013 | 24047863 |
consequences of insect herbivory on grape fine root systems with different growth rates. | herbivory tolerance has been linked to plant growth rate where plants with fast growth rates are hypothesized to be more tolerant of herbivory than slower-growing plants. evidence supporting this theory has been taken primarily from observations of aboveground organs but rarely from roots. grapevines differing in overall rates of new root production, were studied in napa valley, california over two growing seasons in an established vineyard infested with the sucking insect, grape phylloxera (dak ... | 2007 | 17547651 |
foraging activity and seasonal food preference of linepithema micans (hymenoptera: formicidae), a species associated with the spread of eurhizococcus brasiliensis (hemiptera: margarodidae). | linepithema micans (forel) (hymenoptera: formicidae) is the main ant species responsible for the spread of eurhizococcus brasiliensis (wille) (hemiptera: margarodidae), a soil scale that damages vine plants in southern brazil. the daily foraging activity of l. micans and its seasonal preference for protein- and carbohydrate-based foods were evaluated. the study was carried out in a greenhouse using seedlings of the paulsen 1103 rootstock (vitis berlandieri x vitis rupestris) planted individually ... | 2014 | 25195426 |
extracellular plant dna in geneva groundwater and traditional artesian drinking water fountains. | dna, as the signature of life, has been extensively studied in a wide range of environments. while dna analysis has become central to work on natural gene exchange, forensic analyses, soil bioremediation, genetically modified organisms, exobiology, and palaeontology, fundamental questions about dna resistance to degradation remain. this paper investigated on the presence of plant dna in groundwater and artesian fountain (groundwater-fed) samples, which relates to the movement and persistence of ... | 2009 | 19171370 |