| antioxidant activity of polyphenols from seeds of vitis amurensis in vitro. | to study the antioxidant action of five polyphenols (+) catechin, procyanidin b2, procyanidin b5, procyanidin b5 3'-o-gallate, and amurensisin isolated from the seeds of vitis amurensis. | 2000 | 11360672 |
| resistance gene analogs are candidate markers for disease-resistance genes in grape ( vitis spp.). | a set of nbs-containing sequences was isolated from genomic dna of two grape species ( vitis amurensis and vitis riparia) and characterised in a panel of vitis genotypes carrying different levels of resistance against downy mildew and other diseases. a pcr-mediated approach made use of degenerate primers designed on conserved regions encoding known r-genes, and provided the source for cloning grape analogous sequences. cloned sequences were digested with ten endonucleases and 29 out of 71 putati ... | 2002 | 12582885 |
| heyneanol a induces apoptosis via cytochrome c release and caspase activation in human leukemic u937 cells. | heyneanol a, a tetramer of resveratrol, is isolated from the roots of vitis amurensis by cytotoxicity based fractionation. in this study, the mechanism of apoptosis by heyneanol a was evaluated in human leukemic u937 cells. heyneanol a (ic(50) = 6.6 microm at 24 h) exhibited stronger cytotoxic effect than resveratrol (ic(50) = 100 microm at 24 h) by 15-fold on human leukemic u937 cells by xtt assay. apoptotic bodies were observed in u937 cells treated with 6 microm of heyneanol a by tunel assay. ... | 2004 | 14987955 |
| isolation and characterization of a chitinase gene vch3 promoter from grapevine (vitis amurensis). | the 1216-bp 5' upstream region of the gene encoding a class iii chitinase vch3 was isolated from grapevine (vitis amurensis rupr.) by adaptor-pcr (genbank accession number af441123), and the transcriptional start site of the vch3 gene was identified by primer extension, which corresponds to the second a in the dna sequence 5'-atcaagcac-3'. sequence analysis revealed that the vch3 promoter sequence contains caat and tata motifs that are located at the -122 and -29 nucleotide upstream of the trans ... | 2005 | 16222090 |
| colonization by the arbuscular mycorrhizal fungus glomus versiforme induces a defense response against the root-knot nematode meloidogyne incognita in the grapevine (vitis amurensis rupr.), which includes transcriptional activation of the class iii chitinase gene vch3. | inoculation of the grapevine (vitis amurensis rupr.) with the arbuscular mycorrhizal (am) fungus glomus versiforme significantly increased resistance against the root-knot nematode (rkn) meloidogyne incognita. studies using relative quantitative reverse transcription-pcr (rqrt-pcr) analysis of grapevine root inoculation with the am fungus revealed an up-regulation of vch3 transcripts. this increase was greater than that observed following infection with rkn. however, inoculation of the mycorrhiz ... | 2006 | 16326755 |
| roles of salicylic acid-responsive cis-acting elements and w-boxes in salicylic acid induction of vch3 promoter in transgenic tobaccos. | a salicylic acid (sa)-inducible vch3 promoter was recently identified from grapevine (vitis amurensis) that contains two inverse sa-responsive cis-acting elements and four w-boxes. to further demonstrate the roles of these elements, four fragments with lengths from -1187, -892, -589, -276 to +7 bp were fused with the b-glucuronidase (gus) reporter gene and transferred to nicotiana tobacum, together with another four vch3 promoter fragments with mutation in the two inverse sa-responsive elements. ... | 2006 | 16395526 |
| potent inhibition of lewis lung cancer growth by heyneanol a from the roots of vitis amurensis through apoptotic and anti-angiogenic activities. | vitis amurensis rupr. (vitaceae) has long been used in chinese/oriental herbal medicine for the treatment of cancer, but its active compounds and mechanisms of action have not been well studied. to this end, we isolated from its root heyneanol a (ha), which is a tetramer of resveratrol (res), and established the in vivo antitumor activity of ha using the mouse lewis lung carcinoma (llc) model. we administered ha and res by daily intraperitonial injection to c57bl/6 mice that were subcutaneously ... | 2006 | 16675471 |
| the rolb gene-induced overproduction of resveratrol in vitis amurensis transformed cells. | resveratrol is a stilbene, which prevents carcinogenesis at stages of tumor initiation, promotion and progression. in the present investigation, we developed cell cultures of wild-growing grape (vitis amurensis rupr.). the cultures produced low levels of resveratrol, up to 0.026% dry wt., i.e., comparable to levels reported for other plant cell cultures previously established. different methods commonly used to increase secondary metabolite production (cell selection, elicitor treatments and add ... | 2007 | 17166613 |
| resveratrol oligomers from vitis amurensis attenuate beta-amyloid-induced oxidative stress in pc12 cells. | oxidative damage induced by beta-amyloid (abeta) is closely associated with the hallmark pathologies of alzheimer's disease (ad) and may play a critical role in the development of ad. in this study, the protective effects of vitisin a and heyneanol a, resveratrol oligomers isolated from vitis amurensis rupr. (vitaceae), against abeta-induced oxidative cell death were investigated using rat pheochromocytoma (pc12) cells. exposure of pc12 cells to the abeta (20 microm) for 24 h resulted in neurona ... | 2007 | 17541166 |
| antiallergic effects of vitis amurensis on mast cell-mediated allergy model. | in this study, we investigated the effect of the methanol extract of fruits of vitis amurensis rupr. (vitaceae; meva) on the mast cell-mediated allergy model and studied the possible mechanism of action. mast cell-mediated allergic disease is involved in many diseases, such as asthma and sinusitis. the discovery of drugs for the treatment of allergic disease is an important subject in human health. meva inhibited compound 48/80-induced systemic reactions and serum histamine release in a dose-dep ... | 2008 | 18222974 |
| phenylalanine ammonia-lyase and stilbene synthase gene expression in rolb transgenic cell cultures of vitis amurensis. | transformation of vitis amurensis callus culture by the plant oncogene rolb of agrobacterium rhizogenes results in high (up to 3.15% dry wt.) levels of resveratrol in the transformed culture. the present study deals with the effect of rolb on phenylalanine ammonia-lyase (pal) and stilbene synthase (sts) gene expression in two rolb transgenic v. amurensis callus cultures with different levels of rolb expression and resveratrol production. the total expression of pals and stss in rolb transgenic c ... | 2009 | 19043702 |
| enhanced resveratrol accumulation in rolb transgenic cultures of vitis amurensis correlates with unusual changes in cdpk gene expression. | it has been established that transformation of vitis amurensis callus culture with the plant oncogene rolb of agrobacterium rhizogenes results in a high level of resveratrol production in the transformed culture. in the present report, we investigated two rolb transgenic v. amurensis cell cultures with different levels of rolb expression and resveratrol production. we examined whether the calcium ion flux and later steps of the calcium-mediated signal transduction pathway play a role in resverat ... | 2009 | 19285358 |
| the antimicrobial activity of compounds from the leaf and stem of vitis amurensis against two oral pathogens. | nine compounds isolated from the leaf and stem of vitis amurensis rupr. (vitaceae) were evaluated for their antimicrobial activity against two oral pathogens, streptococcus mutans and streptococcus sanguis, which are associated with caries and periodontal disease, respectively. the results of several antimicrobial tests, including mic, mbc, and tbai, showed that three compounds inhibited the growth of the test bacteria at concentrations ranging from 12.5 to 50 microg/ml. among these compounds, c ... | 2010 | 20022753 |
| meoruh wine suppresses mast cell-mediated allergic inflammation. | in this study, we investigated the effect of the meoruh wine (mw), a korean traditional wine made by the fruits of vitis amurensis rupr. (vitaceae) on the mast cell-mediated allergic inflammation and studied the possible mechanism of action. mast cell-mediated allergic disease is involved in many diseases such as anaphylaxis, rhinitis, asthma, and atopic dermatitis. mw inhibited compound 48/80-induced systemic anaphylaxis and serum histamine release in a dose-dependent manner in mice. mw decreas ... | 2011 | 20626232 |
| resveratrol content and expression of phenylalanine ammonia-lyase and stilbene synthase genes in rolc transgenic cell cultures of vitis amurensis. | resveratrol, a naturally occurring polyphenol, has been reported to exhibit a wide range of valuable biological and pharmacological properties. in the present investigation, we show that transformation of vitis amurensis rupr. with the oncogene rolc of agrobacterium rhizogenes increased resveratrol production in the two transformed callus cultures 3.7 and 11.9 times. the rolc-transformed calli were capable of producing 0.099% and 0.144% dry weight of resveratrol. we characterized phenylalanine a ... | 2010 | 20683716 |
| amurensin g, a potent natural sirt1 inhibitor, rescues doxorubicin responsiveness via down-regulation of multidrug resistance 1. | the transition from a chemotherapy-responsive cancer to a chemotherapy-resistant one is accompanied by increased expression of multidrug resistance 1 (mdr1, p-glycoprotein), which plays an important role in the efflux from the target cell of many anticancer agents. we recently showed that a forkhead box-containing protein of the o subfamily 1 (foxo1) is a key regulator of mdr1 gene transcription. because nuclear localization of foxo1 is regulated by silent information regulator two ortholog 1 (s ... | 2010 | 20713551 |
| whole genome wide expression profiles of vitis amurensis grape responding to downy mildew by using solexa sequencing technology. | downy mildew (dm), caused by pathogen plasmopara viticola (pv) is the single most damaging disease of grapes (vitis l.) worldwide. however, the mechanisms of the disease development in grapes are poorly understood. a method for estimating gene expression levels using solexa sequencing of type i restriction-endonuclease-generated cdna fragments was used for deep sequencing the transcriptomes resulting from pv infected leaves of vitis amurensis rupr. cv. zuoshan-1. our goal is to identify genes th ... | 2010 | 21029438 |
| leaf and stem of vitis amurensis and its active components protect against amyloid β protein (25-35)-induced neurotoxicity. | this study investigated a methanol extract from the leaf and stem of vitis amurensis (vitaceae) for possible neuroprotective effects on neurotoxicity induced by amyloid β protein (aβ) (25-35) in cultured rat cortical neurons and also for antidementia activity in mice. exposure of cultured cortical neurons to 10 μm aβ (25-35) for 36 h induced neuronal apoptotic death. at concentrations of 1-10 μg/ml, v. amurensis inhibited neuronal death, the elevation of intracellular calcium ([ca(2+)](i)) and t ... | 2010 | 21052941 |
| construction of a reference linkage map of vitis amurensis and genetic mapping of rpv8, a locus conferring resistance to grapevine downy mildew. | downy mildew, caused by the oomycete plasmopara viticola, is one of the major threats to grapevine. all traditional cultivars of grapevine (vitis vinifera) are susceptible to downy mildew, the control of which requires regular application of fungicides. in contrast, many sources of resistance to p. viticola have been described in the vitis wild species, among which is v. amurensis rupr. (vitaceae), a species originating from east asia. a genetic linkage map of v. amurensis, based on 122 simple s ... | 2011 | 21404060 |
| neuroprotection of the leaf and stem of vitis amurensis and their active compounds against ischemic brain damage in rats and excitotoxicity in cultured neurons. | vitis amurensis (vitaceae) has been reported to have anti-oxidant and anti-inflammatory activities. the present study investigated a methanol extract from the leaf and stem of v. amurensis for neuroprotective effects on cerebral ischemic damage in rats and on excitotoxicity induced by glutamate in cultured rat cortical neurons. transient focal cerebral ischemia was induced by 2h middle cerebral artery occlusion followed by 24h reperfusion (mcao/reperfusion) in rats. orally administered v. amuren ... | 2011 | 21778042 |
| Rpv10: a new locus from the Asian Vitis gene pool for pyramiding downy mildew resistance loci in grapevine. | A population derived from a cross between grapevine breeding strain Gf.Ga-52-42 and cultivar 'Solaris' consisting of 265 F1-individuals was genetically mapped using SSR markers and screened for downy mildew resistance. Quantitative trait locus (QTL) analysis revealed two strong QTLs on linkage groups (LGs) 18 and 09. The locus on LG 18 was found to be identical with the previously described locus Rpv3 and is transmitted by Gf.Ga-52-42. 'Solaris' transmitted the resistance-related locus on LG 09 ... | 2011 | 21935694 |
| resveratrol content and expression of phenylalanine ammonia-lyase and stilbene synthase genes in cell cultures of vitis amurensis treated with coumaric acid. | the present study examines the effect of p-coumaric acid (ca), a precursor of stilbenes and isoflavonoids, on biosynthesis of resveratrol in cell cultures of vitis amurensis. earlier, we transformed v. amurensis with the rolb gene of agrobacterium rhizogenes and showed increased level of resveratrol production in the rolb transgenic cell culture. we used control and the rolb-transgenic cell culture of v. amurensis as a model system in this study. ca was capable of increasing resveratrol producti ... | 2011 | 21938424 |
| involvement of dna methylation in the regulation of sts10 gene expression in vitis amurensis. | dna methylation is known to play an important role in various developmental processes and defense mechanisms in plants and other organisms. however, it is not known whether dna methylation is implicated in the genetic regulation of plant secondary metabolism, including resveratrol biosynthesis. resveratrol is a naturally occurring polyphenol that is present in grapes, peanuts, and other plant sources, and it exhibits a wide range of valuable biologically active properties. the transformation of ... | 2013 | 23179442 |
| overexpression of a thaumatin-like protein gene from vitis amurensis improves downy mildew resistance in vitis vinifera grapevine. | downy mildew is a highly destructive disease in grapevine production. a gene encoding pathogenesis-related (pr) thaumatin-like protein was isolated from the downy mildew-resistant grapevine "zuoshan-1," a clonal selection from wild vitis amurensis rupr. the predicted thaumatin-like protein (vatlp) has 225 amino acids and it is acidic, with a calculated isoelectric point of 4.8. the full length of the vatlp gene was transformed into somatic embryogenic calli of v. vinifera 'thompson seedless' via ... | 2016 | 27900595 |
| immobilization of xylanase purified from bacillus pumilus vlk-1 and its application in enrichment of orange and grape juices. | this study was conducted to evaluate the efficacy of purified free and immobilized xylanase in enrichment of fruit juices. extracellular xylanase produced from bacillus pumilus vlk-1 was purified to apparent homogeneity by 15.4-fold with 88.3 % recovery in a single step using cm-sephadex c-50. purified xylanase showed a single band on sds-polyacrylamide gel with a molecular mass of 22.0 kda. the purified enzyme was immobilized on glutaraldehyde-activated aluminum oxide pellets and the immobiliza ... | 2014 | 25190829 |
| the grapevine basic helix-loop-helix (bhlh) transcription factor positively modulates cbf-pathway and confers tolerance to cold-stress in arabidopsis. | basic helix-loop-helix (bhlh)-type transcription factors play diverse roles in plant physiological response and stress-adaptive regulation network. here, we identified one grapevine bhlh transcription factor from a cold-tolerant accession 'heilongjiang seedling' of chinese wild vitis amurensis (vabhlh1) as a transcriptional activator involved in cold stress. we also compared with its counterpart from a cold-sensitive vitis vinifera cv. cabernet sauvignon (vvbhlh1). these two putative proteins ar ... | 2014 | 24859977 |
| overexpression of a stress-responsive u-box protein gene vapub affects the accumulation of resistance related proteins in vitis vinifera 'thompson seedless'. | many u-box proteins have been identified and characterized as important factors against environmental stresses such as chilling, heat, salinity and pathogen attack in plant. our previous research reported the cloning of a novel u-box protein gene vapub from vitis amurensis 'zuoshanyi' grape and suggested a function of it in related to cold stress in the model plant arabidopsis system. in this study, the role of vapub in response to biotic and abiotic stress was further analyzed in the homologous ... | 2017 | 28039816 |
| overexpression of pathogen-induced grapevine tir-nb-lrr gene varga1 enhances disease resistance and drought and salt tolerance in nicotiana benthamiana. | the nbs-lrr proteins encoded by the majority of r genes represent important intracellular receptors that directly or indirectly recognize pathogen effector proteins, which subsequently activate plant defense responses. in this study, a novel plasmopara viticola-induced tir-nbs-lrr gene, named varga1, was cloned from leaf tissues of a highly downy mildew-resistant vitis amurensis "shuanghong" grapevine. the fluorescence signal of the varga1-gfp fusion protein was clearly partitioned to the cytopl ... | 2017 | 27468994 |
| comparative transcriptome analysis reveals defense-related genes and pathways against downy mildew in vitis amurensis grapevine. | downy mildew (dm), caused by oomycete plasmopara viticola (pv), can lead to severe damage to vitis vinifera grapevines. vitis amurensis has generally been regarded as a dm resistant species. however, when v. amurensis 'shuanghong' were inoculated with pv strains 'zj-1-1' and 'jl-7-2', the former led to obvious dm symptoms (compatible), while the latter did not develop any dm symptoms but exhibited necrosis (incompatible). in order to underlie molecular mechanism in dm resistance, mrna-seq based ... | 2015 | 26151858 |
| historical introgression of the downy mildew resistance gene rpv12 from the asian species vitis amurensis into grapevine varieties. | the amur grape (vitis amurensis rupr.) thrives naturally in cool climates of northeast asia. resistance against the introduced pathogen plasmopara viticola is common among wild ecotypes that were propagated from manchuria into chinese vineyards or collected by soviet botanists in siberia, and used for the introgression of resistance into wine grapes (vitis vinifera l.). a qtl analysis revealed a dominant gene rpv12 that explained 79% of the phenotypic variance for downy mildew resistance and was ... | 2013 | 23593440 |
| vaerd15, a transcription factor gene associated with cold-tolerance in chinese wild vitis amurensis. | early responsive to dehydration (erd) genes can be rapidly induced to counteract abiotic stresses, such as drought, low temperatures or high salinities. here, we report on an erd gene (vaerd15) related to cold tolerance from chinese wild vitis amurensis accession 'heilongjiang seedling'. the full-length vaerd15 cdna is 685 bp, including a 66 bp 5'-untranslated region (utr), a 196 bp 3'-utr region and a 423 bp open reading frame encoding 140 amino acids. the vaerd15 protein shares a high amino ac ... | 2017 | 28326090 |
| the methylation status of plant genomic dna influences pcr efficiency. | during the polymerase chain reaction (pcr), which is a versatile and widely used method, certain dna sequences are rapidly amplified through thermocycling. although there are numerous protocols of pcr optimization for different applications, little is known about the effect of dna modifications, such as dna methylation, on pcr efficiency. recent studies show that cytosine methylation alters dna mechanical properties and suggest that dna methylation may directly or indirectly influence the effect ... | 2015 | 25506767 |
| vam3, a compound derived from vitis amurensis rupr., attenuated colitis-related tumorigenesis by inhibiting nf-κb signaling pathway. | chronic inflammation is one of the important mediators of colitis-related colon cancer (crc). abundant mast cells (mcs) were observed in the tumor microenvironment and mediators released upon mc activation play an important role in the process of chronic inflammation. previously, we found that activation of intestine mucosal mcs recruited and modulated the inflammatory cd11b(+)gr1(+) cells to promote the crc development. in the current study we investigated the effects of vam3, a resveratrol dim ... | 2016 | 27679575 |
| phytohormone and genome variations in vitis amurensis resistant to downy mildew. | downy mildew (dm) resistance is a highly desirable agronomic trait in grape breeding. high variation in plasmopara viticola resistance was found in vitis cultivars. some accessions show high p. viticola resistance even under conditions highly conducive to dm. here, leaf disc inoculation experiments revealed that vitis amurensis 'zuoshaner' exhibited dm resistance with necrotic spots, whereas the v. amurensis × v. vinifera hybrid cultivar 'zuoyouhong' was susceptible. changes in plant hormones ac ... | 2017 | 28727939 |
| characterisation of seed oils from different grape cultivars grown in china. | to explore the potential of the large amount of grape pomace in wineries of china, oils of three eurasian grape cultivars (chardonnay, merlot and carbernet sauvignon) and two chinese traditional grape cultivars (vitis amurensis and vitis davidii), were characterised. the results showed seed oil properties differ for various grape varities. grape seed oils were demonstrated to be good sources of polyunsaturated fatty acid (pufa) (63.88-77.12 %), sterols (227.99-338.83 mg/100 g oil) and tocotrieno ... | 0 | 27765984 |
| overexpression of muscadinia rotundifolia cbf2 gene enhances biotic and abiotic stress tolerance in arabidopsis. | c-repeat-binding factor dehydration-responsive element-binding factor 1c (cbf2/dreb1c) gene encodes a small family of transcriptional activator that has been described as playing an important role in freezing tolerance and cold acclimation of plants. we here report that cbf2 gene also plays an important role in the early response to the pathogen infection of grapevine downy mildew disease. the expression level of cbf2 increased dramatically and reached a peak at 7 h after infection in immune gra ... | 2017 | 26795343 |
| expression of the grape vasts19 gene in arabidopsis improves resistance to powdery mildew and botrytis cinerea but increases susceptibility to pseudomonas syringe pv tomato dc3000. | stilbene synthase (sts) is a key enzyme that catalyzes the biosynthesis of resveratrol compounds and plays an important role in disease resistance. the molecular pathways linking sts with pathogen responses and their regulation are not known. we isolated an sts gene, vasts19, from a chinese wild grape, vitis amurensis rupr. cv. "tonghua-3", and transferred this gene to arabidopsis. we then generated vasts19-expressing arabidopsis lines and evaluated the functions of vasts19 in various pathogen s ... | 2017 | 28926983 |
| isolation and structural identification of the main anthocyanin monomer in vitis amurensis rupr. | many types of anthocyanins are present in vitis amurensis rupr of 'beibinghong', which is grown in north-east china and has high antioxidant activity. however, the anthocyanin with the highest content in v. amurensis rupr has not yet been identified. in this study, pulsed electric field extraction and semi-preparative liquid phase separation were used to isolate the anthocyanin monomer from 'beibinghong'. uv-vis spectroscopy, ftir spectroscopy, mass spectra and nuclear magnetic resonance were us ... | 2018 | 28782379 |
| an efficient method for transgenic callus induction from vitis amurensis petiole. | transformation is the main platform for genetic improvement and gene function studies in plants. however, the established somatic embryo transformation system for grapevines is time-consuming and has low efficiency, which limits its utilization in functional genomics research. vitis amurensis is a wild vitis species with remarkable cold tolerance. the lack of an efficient genetic transformation system for it has significantly hindered the functional identification of cold stress related genes in ... | 2017 | 28640905 |
| optimisation of pulsed electric fields extraction of anthocyanin from beibinghong vitis amurensis rupr. | beibinghong vitis amurensis rupr has wide plantation area, high productivity and rich anthocyanin. common hot-extraction has poor deficiency and destroys anthocyanin severely. for beibinghong v. amurensis rupr as materials, response surface-optimised electric fields were used, the structure of beibinghong was observed by sem, antioxidant activity was measured by dpph, abts and reducing force, the component of anthocyanin was analyzed by hplc-ms. we found the content of total anthocyanin extracte ... | 2018 | 28480755 |
| stilbene content and expression of stilbene synthase genes in cell cultures of vitis amurensis treated with cinnamic and caffeic acids. | it has previously been shown that exogenous application of p-coumaric acid (ca), a precursor of phenolic compounds, improved stilbene production in cell cultures of vitis amurensis. this study examines the effect of cinnamic (cin) and caffeic (caf) acids, which are also phenolic precursors, on stilbene biosynthesis in the cell cultures. five stilbenes, t-resveratrol diglucoside, t-piceid (t-resveratrol glucoside), t-resveratrol, t-ε-viniferin, and t-δ-viniferin, were found in the treated and unt ... | 2017 | 28332216 |
| the synthesis and accumulation of resveratrol are associated with veraison and abscisic acid concentration in beihong (vitis vinifera × vitis amurensis) berry skin. | resveratrols are polyphenolic secondary metabolites that can benefit human health, and only occur in a few plant families including vitaceae. it has been reported that abscisic acid (aba) can induce veraison (the onset of grape berry ripening) and may induce the accumulation of resveratrol in berry skin. however, the relationships between aba, veraison, the accumulation of anthocyanins and the accumulation of resveratrol in the berry are poorly understood. this study attempted to answer this que ... | 2016 | 27857716 |
| stilbene accumulation and expression of stilbene biosynthesis pathway genes in wild grapevine vitis amurensis rupr. | we detected and quantified six stilbenes ( cis -piceid, t -piceid, t -ε-viniferin, cis -ε-viniferin, t -resveratrol, and t -δ-viniferin) in the leaves, petioles, berry skins, and seeds of wild-growing vitis amurensis . the highest content of stilbenes and expression of stilbene biosynthesis genes were in the probes collected in the autumn and after ultraviolet elicitation. stilbenes, including the best-studied stilbene resveratrol, are known to display valuable bioactivities and protect plants a ... | 2017 | 27686467 |
| antioxidant and hepatoprotective effects of procyanidins from wild grape (vitis amurensis) seeds in ethanol-induced cells and rats. | in the present study, we characterized the antioxidant and hepatoprotective mechanisms underlying of wild grape seed procyanidins (wgp) against oxidative stress damage in ethanol-treated hepg2 cell and sprague-dawley (sd)-rat models. in hepg2 cells, wgp not only diminished the ethanol (etoh, 100 mm)-induced reactive oxygen species (ros) formation and cytochrome p450 2e1 (cyp2e1) expression, but also renovated both the activity and expression of antioxidant enzymes including catalase, superoxide ... | 2016 | 27213339 |
| expression of vitis amurensis nac26 in arabidopsis enhances drought tolerance by modulating jasmonic acid synthesis. | the growth and fruit quality of grapevines are widely affected by abnormal climatic conditions such as water deficits, but many of the precise mechanisms by which grapevines respond to drought stress are still largely unknown. here, we report that vanac26, a member of the nac transcription factor family, was upregulated dramatically during cold, drought and salinity treatments in vitis amurensis, a cold and drought-hardy wild vitis species. heterologous overexpression of vanac26 enhanced drought ... | 2016 | 27162276 |
| phenolic and chromatic properties of beibinghong red ice wine during and after vinification. | the phenolic and chromatic characteristics of a special red ice wine made from a vitis amurensis × v. vinifera hybrid cultivar beibinghong were studied. results from two different vintages (2013 and 2014) showed that during vinification, the phenolic acid content increased, while the level of flavonoids (flavonols, flavan-3-ols, and anthocyanins) reduced by a variable extent. the color intensity and red % decreased together with a decrease in anthocyanin content. this was accompanied by an incre ... | 2016 | 27104511 |
| overexpression of vapat1, a gras transcription factor from vitis amurensis, confers abiotic stress tolerance in arabidopsis. | vapat1 functions as a stress-inducible gras gene and enhanced cold, drought and salt tolerance in transgenic arabidopsis via modulation of the expression of a series of stress-related genes. the plant-specific gras transcription factor family regulates diverse processes involved in plant growth, development and stress responses. in this study, vapat1, a gras gene from vitis amurensis was isolated and functionally characterized. sequence alignment and phylogenetic analysis showed that vapat1 has ... | 2016 | 26687967 |
| pharmacokinetic analysis and tissue distribution of vam3 in the rat by a validated lc-ms/ms method. | vam3 is a potential pharmacologically active ingredient isolated from vitis amurensis rupr. a rapid, simple and sensitive method to determine vam3 levels in rat plasma and tissue was developed based on lc-ms/ms. vam3 and an internal standard (is) were chromatographed on a c18 short column with acetonitrile-0.1% formic acid in water by gradient elution. ms detection was performed by electrospray ionization in negative ion multiple reaction-monitoring modes. this method monitored the transitions m ... | 2015 | 26579454 |
| rapid separation and identification of phenolics in crude red grape skin extracts by high performance liquid chromatography coupled to diode array detection and tandem mass spectrometry. | a rapid and efficient method was established for the simultaneous determination of structures and configurations for 45 phenolics isolated from crude red grape skin extracts without extensive sample preparation. separation and compound assignments were achieved using high performance liquid chromatography coupled to diode array detection and tandem mass spectrometry (hplc-dad-ms(2)). a poroshell 120 ec-c18 (100mm×3.0mm, 2.7μm) column was employed to separate the phenolics, which were eluted usin ... | 2015 | 26338211 |
| vacpk20, a calcium-dependent protein kinase gene of wild grapevine vitis amurensis rupr., mediates cold and drought stress tolerance. | abiotic stresses, such as drought, salinity, cold and heat, are major environmental factors that limit crop productivity. vitis amurensis rupr. is a wild grapevine species displaying a high level of abiotic and biotic stress resistance. protein kinases, including ca(2+)-dependent protein kinases (cdpks), are known to mediate plant acclimation to various environmental changes. however, the functions of most grape cdpks have not been clarified. a recent cdpk gene expression analysis revealed that ... | 2015 | 26264965 |
| molecular and biochemical characterization of the udp-glucose: anthocyanin 5-o-glucosyltransferase from vitis amurensis. | generally, red vitis vinifera grapes only contain monoglucosidic anthocyanins, whereas most non-vinifera red grapes of the vitis genus have both monoglucosidic and bis-glucosidic anthocyanins, the latter of which are believed to be more hydrophilic and more stable. although previous studies have established the biosynthetic mechanism for formation of monoglucosidic anthocyanins, less attention has been paid to that of bis-glucosidic anthocyanins. in the present research, the full-length cdna of ... | 2015 | 26159788 |
| using specific length amplified fragment sequencing to construct the high-density genetic map for vitis (vitis vinifera l. × vitis amurensis rupr.). | in this study, 149 f1 plants from the interspecific cross between 'red globe' (vitis vinifera l.) and 'shuangyou' (vitis amurensis rupr.) and the parent were used to construct a molecular genetic linkage map by using the specific length amplified fragment sequencing technique. dna sequencing generated 41.282 gb data consisting of 206,411,693 paired-end reads. the average sequencing depths were 68.35 for 'red globe,' 63.65 for 'shuangyou,' and 8.01 for each progeny. in all, 115,629 high-quality s ... | 2015 | 26089826 |
| a novel system for evaluating drought-cold tolerance of grapevines using chlorophyll fluorescence. | grape production in continental climatic regions suffers from the combination of drought and cold stresses during winter. developing a reliable system to simulate combined drought-cold stress and to determine physiological responses and regulatory mechanisms is important. evaluating tolerance to combined stress at germplasm level is crucial to select parents for breeding grapevines. | 2015 | 25849490 |
| salicylic acid induces alterations in the methylation pattern of the vasts1, vasts2, and vasts10 genes in vitis amurensis rupr. cell cultures. | salicylic acid (sa) treatment selectively reduced the cytosine dna methylation of stilbene synthase ( sts ) genes and stimulated resveratrol production in cell cultures of vitis amurensis. the effect of salicylic acid (sa) on plant growth, flowering time, and fruit number is known to correlate with the level of dna methylation, while the potential correlation between sa-induced changes in dna methylation and biosynthesis of secondary metabolites has not been studied. trans-resveratrol, a natural ... | 2015 | 25420769 |
| regulation of resveratrol production in vitis amurensis cell cultures by calcium-dependent protein kinases. | resveratrol is a naturally occurring plant stilbene that exhibits a wide range of valuable biological and pharmacological properties. although the beneficial effects of trans-resveratrol to human health and plant protection against fungal pathogens are well-established, little is known about the molecular mechanisms regulating stilbene biosynthesis in plant cells. it has been recently shown that overexpression of the calcium-dependent protein kinase vacpk20 gene considerably increased resveratro ... | 2015 | 25410806 |
| protective effects of resveratrol oligomers from vitis amurensis against sodium nitroprusside-induced neurotoxicity in human neuroblastoma sh-sy5y cells. | nitric oxide (no) induces apoptosis in neuronal cells, and has been implicating in a variety of neuronal pathological process. thus, there is much interest in identifying natural substances which have protective effects against damage induced by nitrosative stress. the roots of vitis amurensis have been used as traditional medicine and contain structurally diverse resveratrol oligomers with various biological activities. however, there have been few studies on the protective effect of resveratro ... | 2015 | 25348868 |
| vitis amurensis ruprecht root inhibited α-melanocyte stimulating hormone-induced melanogenesis in b16f10 cells. | the root of vitis amurensis ruprecht, a sort of wild-growing grape, has been used in oriental medicine for treatment of skin ailments; however, its dermatological activity is not sufficiently understood. the aim of this study was to investigate tyrosinase inhibitory and anti-melanogenic activities of v. amurensis ruprecht root methanol extract (varm) in b16f10 mouse melanoma cells and to attempt to isolate and identify the active compound issued from varm. | 2014 | 25324929 |
| transcriptome profiling of vitis amurensis, an extremely cold-tolerant chinese wild vitis species, reveals candidate genes and events that potentially connected to cold stress. | vitis amurensis rupr. is an exceptional wild-growing vitis (grape) species that can safely survive a wide range of cold conditions, but the underlying cold-adaptive mechanism associated with gene regulation is poorly investigated. we have analyzed the physiochemical and transcriptomic changes caused by cold stress in a cold-tolerant accession, 'heilongjiang seedling', of chinese wild v. amurensis. we statistically determined that a total of 6,850 cold-regulated transcripts were involved in cold ... | 2014 | 25190283 |
| linkage of cold acclimation and disease resistance through plant-pathogen interaction pathway in vitis amurensis grapevine. | low temperatures cause severe damage to none cold hardy grapevines. a preliminary survey with solexa sequencing technology was used to analyze gene expression profiles of cold hardy vitis amurensis 'zuoshan-1' after cold acclimation at 4 °c for 48 h. a total of 16,750 and 18,068 putative genes were annotated for 4 °c-treated and control library, respectively. among them, 393 genes were upregulated for at least 20-fold, while 69 genes were downregulated for at least 20-fold under the 4 °c treatme ... | 2014 | 25154381 |
| chinese wild-growing vitis amurensis ice1 and ice2 encode myc-type bhlh transcription activators that regulate cold tolerance in arabidopsis. | winter hardiness is an important trait for grapevine breeders and producers, so identification of the regulatory mechanisms involved in cold acclimation is of great potential value. the work presented here involves the identification of two grapevine ice gene homologs, vaice1 and vaice2, from an extremely cold-tolerant accession of chinese wild-growing vitis amurnensis, which are phylogenetically related to other plant ice1 genes. these two structurally different ice proteins contain previously ... | 2014 | 25019620 |
| vam3, a resveratrol dimer, inhibits cigarette smoke-induced cell apoptosis in lungs by improving mitochondrial function. | to investigate the effects of vam3 (a resveratrol dimer extracted from vitis amurensis rupr) on cigarette smoke (cs)-induced cell apoptosis in lungs in vitro and in vivo and the underlying mechanisms of action. | 2014 | 24747163 |
| identification of resveratrol oligomers as inhibitors of cystic fibrosis transmembrane conductance regulator by high-throughput screening of natural products from chinese medicinal plants. | inhibitors of cystic fibrosis transmembrane conductance regulator (cftr) have been widely used for characterizing cftr function in epithelial fluid transport and in diseases such as secretory diarrhea, polycystic kidney disease and cystic fibrosis. few small molecule cftr inhibitors have been discovered so far from combinatorial compound library. in the present study, we used a high throughput screening (hts)-based natural product discovery strategy to identify new cftr inhibitors from chinese m ... | 2014 | 24714160 |
| betulinic acid isolated from vitis amurensis root inhibits 3-isobutyl-1-methylxanthine induced melanogenesis via the regulation of mek/erk and pi3k/akt pathways in b16f10 cells. | previously, betulinic acid was identified as one of the main compounds responsible for the anti-melanogenic effect in vitis amurensis root. in this study, we investigated the precise mechanism underlying the anti-melanogenic activity of betulinic acid in b16f10 cells. betulinic acid significantly attenuated 3-isobutyl-1-methylxanthine (ibmx)-induced melanin production by inhibiting tyrosinase, tyrosinase related protein (trp)-1, and trp-2 expression through the modulation of their corresponding ... | 2014 | 24632067 |
| vacpk20 gene overexpression significantly increased resveratrol content and expression of stilbene synthase genes in cell cultures of vitis amurensis rupr. | resveratrol, a naturally occurring plant phenol, has been reported to exhibit a wide range of valuable biological and pharmacological properties. in the present investigation, we show that transformation of a vitis amurensis rupr. cell suspension with the gene vacpk20 for a calcium-dependent protein kinase (cdpk) under the control of double camv 35s promoter increased resveratrol production in five independently transformed cell lines in 9-68 times compared with control cells. the vacpk20-transf ... | 2014 | 24584516 |
| [chromosome variability in grape (vitis amurensis rupr.) cells transformed with plant oncogene rolb]. | the numbers of chromosomes and nucleoli in cultured cells of vitis amurensis transformed with the rolb oncogene from a. rhizogenes have been studied. in general, the integration of the rolb gene in grape dna mostly caused the elevation of the level of the chromosome variability, as well as higher numbers of nucleoli in the cultured cells. the possible influence of the observed chromosomal modifications on the productivity parameters of the grape cell cultures is discussed. | 2013 | 24450194 |
| anti-inflammatory effect of procyanidins from wild grape (vitis amurensis) seeds in lps-induced raw 264.7 cells. | in the present study, the anti-inflammatory effect and underlying mechanisms of wild grape seeds procyanidins (wgp) were examined using lipopolysaccharide- (lps-) stimulated raw 264.7 cells. we used nitric oxide (no) and prostaglandin e2 (pge2) and reactive oxygen species (ros) assays to examine inhibitory effect of wgp and further investigated the mechanisms of wgp suppressed lps-mediated genes and upstream expression by western blot and confocal microscopy analysis. our data indicate that wgp ... | 2013 | 24260615 |
| separation of five oligostilbenes from vitis amurensis by flow-rate gradient high-performance counter-current chromatography. | a rapid and efficient high-performance counter-current chromatography (hpccc) method was developed to separate five oligostilbenes from the roots of vitis amurensis. an n-hexane/ethyl acetate/methanol/water system (4:8:4:10, v/v/v/v) was selected as an optimal two-phase solvent system of which the upper phase was used as the stationary phase and the lower phase was used as the mobile one. partition coefficient values for the target compounds under these optimized conditions were 0.28 (1, ampleos ... | 2013 | 24167107 |
| investigation of the distribution and season regularity of resveratrol in vitis amurensis via hplc-dad-ms/ms. | this study aims to investigate the change trend of resveratrol contents in different tissues of vitis amurensis rupr. during the different seasons in a year. a rapid and sensitive method using high-performance liquid chromatography coupled with diode array detection and tandem mass spectrometry was developed. resveratrol is mainly distributed in the rhizomes and roots of grape plants. it is also found in leaves and vines, but to a lesser extent. resveratrol contents are augmented gradually in rh ... | 2014 | 24001812 |
| stress-responsive gene ice1 from vitis amurensis increases cold tolerance in tobacco. | we report the identification of the inducer of cbf expression 1 (ice1) from vitis amurensis, an upstream transcription factor that regulates the transcription of cbf-like genes. the structure of the basic helix-loop-helix domain of vaice1 is closely related to that of ice1 in woody plants. this gene is strongly induced in leaves, roots, stems, and petioles by cold temperature. with longer duration of exposure to cold treatments, the expression patterns of organs exhibit differences, which are no ... | 2013 | 23968929 |
| expression of calcium-dependent protein kinase (cdpk) genes under abiotic stress conditions in wild-growing grapevine vitis amurensis. | calcium-dependent protein kinases (cdpks), which are important sensors of ca(2+) flux in plants, are known to play essential roles in plant development and adaptation to abiotic stresses. in the present work, we studied expression of cdpk genes under osmotic and temperature stress treatments in wild-growing grapevine vitis amurensis rupr., which is known to possess high adaptive potential and a high level of resistance against adverse environmental conditions. in this study, using rt-pcr with de ... | 2013 | 23886738 |
| a new stilbene dimer from vitis amurensis. | one new stilbene dimer named amurensin o (1), together with one known resveratrol trimer melapinol b (2), was isolated from vitis amurensis, and their structures were identified on the basis of spectral analysis, especially 2d nmr spectral analysis. | 2013 | 23777455 |
| effect of trans-ε-viniferin on in vitro porcine oocyte maturation and subsequent developmental competence in preimplantation embryos. | trans-ε-viniferin is a naturally occurring polyphenol belonging to the stilbenoid family that has been isolated from vitis amurensis, one of the most common wild grapes in asia. we investigated the effects of trans-ε-viniferin on in vitro maturation (ivm) and developmental competence after in vitro fertilization (ivf) or parthenogenesis (pa). we observed that trans-ε-viniferin treatment during ivm did not improve nuclear maturation rates of oocytes in any group, but significantly increased (p<0. ... | 2013 | 23698084 |
| differences in the methylation patterns of the vasts1 and vasts10 genes of vitis amurensis rupr. | resveratrol is a plant-derived phenol but the mechanism that regulates its biosynthesis remains unidentified. stilbene synthase (sts) catalyzes resveratrol formation in vivo and we have proposed that inducers of resveratrol production affect sts expression through an unidentified epigenetic mechanism. to investigate the role of dna methylation in resveratrol biosynthesis, we treated both rolb transgenic and empty vector control vitis amurensis cell cultures with the dna demethylation agent, 5-az ... | 2013 | 23690043 |
| [effect of plant stilbene precursors on the biosynthesis of resveratrol in vitis amurensis rupr. cell cultures]. | the biosynthesis of resveratrol after the application of a precursor for biosynthesis, i.e., phenylalanine (phe), has been studied. the application of phe has been shown to increase significantly the expression of the phenylalanine-ammonia-lyase (pal) and stilbene synthase (sts) genes and enhance the production of resveratrol by 8.5 times. data on resveratrol production after the addition of phe and coumaric acid (ca) were compared with known analogs. | 2013 | 23662452 |
| construction of a cdna library of the chinese wild vitis amurensis under cold stress and analysis of potential hardiness-related expressed sequence tags. | a cdna library of chinese wild vitis amurensis, which is the most cold-resistant species in the genus vitis, was constructed using young leaves of seedlings of the clone heilongjiang potted and subjected to cold stress. the leaves were harvested at various times after cold stress for total rna extraction, which was used to generate expressed sequence tags (ests). the titer of the original library was 2.67 x 10(6) pfu/ml and the corresponding combination frequency was 98.5%. the test values of th ... | 2013 | 23661443 |
| genome wide transcriptional profile analysis of vitis amurensis and vitis vinifera in response to cold stress. | grape is one of the most important fruit crops worldwide. the suitable geographical locations and productivity of grapes are largely limited by temperature. vitis amurensis is a wild grapevine species with remarkable cold-tolerance, exceeding that of vitis vinifera, the dominant cultivated species of grapevine. however, the molecular mechanisms that contribute to the enhanced freezing tolerance of v. amurensis remain unknown. here we used deep sequencing data from restriction endonuclease-genera ... | 2013 | 23516547 |
| amurensin g induces autophagy and attenuates cellular toxicities in a rotenone model of parkinson's disease. | although parkinson's disease is a common neurodegenerative disorder its cause is still unknown. recently, several reports showed that inducers of autophagy attenuate cellular toxicities in parkinson's disease models. in this report we screened hek293 cells that stably express gfp-lc3, a marker of autophagy, for autophagy inducers and identified amurensin g, a compound isolated from the wild grape (vitis amurensis). amurensin g treatment induced punctate cytoplasmic expression of gfp-lc3 and incr ... | 2013 | 23485458 |
| stilbenoid profiles of canes from vitis and muscadinia species. | we present stilbenoid profiles of canes from 16 grapevines. fifteen stilbenoids were obtained through isolation and structure identification using ms, nmr, and [α](d) or as commercial standards. an hplc-uv method for the simultaneous quantification of nine of these stilbenoids was developed and applied to canes of vitis amurensis, vitis arizonica, vitis berlandieri, vitis betulifolia, vitis cinerea, vitis × champini, vitis × doaniana, vitis labrusca, vitis candicans (syn. vitis mustangensis), vi ... | 2013 | 23270496 |
| structure and expression profiling of a novel calcium-dependent protein kinase gene, cdpk3a, in leaves, stems, grapes, and cell cultures of wild-growing grapevine vitis amurensis rupr. | key message : vacdpk3a is actively expressed in leaves, stems, inflorescences, and berries of vitis amurensis and may act as a positive growth regulator, but is not involved in the regulation of resveratrol biosynthesis. calcium-dependent protein kinases (cdpks) are known to play important roles in plant development and defense against biotic and abiotic stresses. it has previously been shown that cdpk3a is the predominant cdpk transcript in cell cultures of wild-growing grapevine vitis amurensi ... | 2013 | 23233131 |
| potent vasodilation effect of amurensin g is mediated through the phosphorylation of endothelial nitric oxide synthase. | endothelial nitric oxide synthase (enos) has important regulatory functions in vascular tone, and impaired endothelium-dependent vasodilatation is a key event in diabetes and atherosclerosis. vitis amurensis grapes containing resveratrol oligomers are consumed as wine and fruit and have antioxidative and neuroprotective effects. in this study, our goal was identify the most potent enos-activating compound among six stilbenes and oligostilbenes found in v. amurensis and to clarify its molecular m ... | 2012 | 22982565 |
| amurensin g inhibits angiogenesis and tumor growth of tamoxifen-resistant breast cancer via pin1 inhibition. | acquired resistance to tamoxifen (tam) is a serious therapeutic problem among estrogen-receptor-positive breast cancer patients. we have previously reported that tam-resistant mcf-7 (tamr-mcf-7) cells have elevated angiogenic potential via pin1-dependent vascular endothelial growth factor (vegf) production. vitis amurensis grape consumed as wine and fruit contains several resveratrol-like stilbenes or oligostilbenes. in this study, we screened for the most active compound to inhibit vegf product ... | 2012 | 22842120 |
| mapping of crown gall resistance locus rcg1 in grapevine. | agrobacteria are efficient plant pathogens. they are able to transform plant cells genetically resulting in abnormal cell proliferation. cultivars of vitis vinifera are highly susceptible to many virulent agrobacterium strains but certain wild vitis species, including vitis amurensis have resistant genotypes. studies of the molecular background of such natural resistance are of special importance, not only for practical benefits in agricultural practice but also for understanding the role of pla ... | 2012 | 22801874 |
| anthocyanin identification and composition of wild vitis spp. accessions by using lc-ms and lc-nmr. | the composition and concentration of anthocyanins of grape berry skins were analyzed in order to assess phenotypic variation between four grape wine varieties belonging to 4 different species: vitis vinifera, vitis amurensis, vitis cinerea and vitis x champinii. high-performance liquid chromatography coupled to mass spectrometry (lc-ms) and nmr spectroscopy (lc-nmr) were used to separate and identify the structure of anthocyanins present in these species. combination of lc-ms and lc-nmr data res ... | 2012 | 22688046 |
| identification of micrornas from amur grape (vitis amurensis rupr.) by deep sequencing and analysis of microrna variations with bioinformatics. | microrna (mirna) is a class of functional non-coding small rna with 19-25 nucleotides in length while amur grape (vitis amurensis rupr.) is an important wild fruit crop with the strongest cold resistance among the vitis species, is used as an excellent breeding parent for grapevine, and has elicited growing interest in wine production. to date, there is a relatively large number of grapevine mirnas (vv-mirnas) from cultivated grapevine varieties such as vitis vinifera l. and hybrids of v. vinife ... | 2012 | 22455456 |
| procyanidins from wild grape (vitis amurensis) seeds regulate are-mediated enzyme expression via nrf2 coupled with p38 and pi3k/akt pathway in hepg2 cells. | procyanidins, polymers of flavan-3-ol units, have been reported to exhibit many beneficial health effects such as antioxidant and anti-carcinogenic effects. in this study, we investigated the cancer chemopreventive properties of procyanidins from wild grape (vitis amurensis) seeds in particular their roles in inducing phase ii detoxifying/antioxidant enzymes as well as in modulating the upstream kinases. ethanolic extract of v. amurensis seeds was fractionated with a series of organic solvents a ... | 2012 | 22312287 |
| photosynthetic responses to heat treatments at different temperatures and following recovery in grapevine (vitis amurensis l.) leaves. | the electron transport chain, rubisco and stomatal conductance are important in photosynthesis. little is known about their combined responses to heat treatment at different temperatures and following recovery in grapevines (vitis spp.) which are often grown in climates with high temperatures. | 2011 | 21887227 |
| anthocyanins profile of grape berries of vitis amurensis, its hybrids and their wines. | anthocyanins are responsible for the color of grapes and wine, an important attribute of their quality. many authors have used anthocyanins profile to classify the grape cultivars and wine authenticity. the anthocyanin profiles of grape berries of vitis amurensis, its hybrids and their wines were analyzed by hplc-esi-ms/ms. the results identified 17 anthocyanins in these grape cultivars, including 11 anthocyanin monoglucosides (five pyranoanthocyanin monoglucosides and one acylated pyranoanthocy ... | 2010 | 20559511 |
| antioxidant and lipoxygenase inhibitory activity of oligostilbenes from the leaf and stem of vitis amurensis. | the root and stem of vitis amurensis (vitaceae) have popularly used as traditional medicine for treatment of cancer and various pains in korea and japan. recent studies, its root and stem possess anti-inflammatory, anti-tumor activities, and protective effects against beta-amyloid-induced oxidative stress. | 2009 | 19560532 |
| stilbenes and oligostilbenes from leaf and stem of vitis amurensis and their cytotoxic activity. | chromatographic separation of the etoac fraction from the leaf and stem of vitis amurensis led to the isolation of six oligostilbenoids (i.e., r-2-viniferin (1), trans-amurensin b (2), trans-epsilon-viniferin (3), gnetin h (4), amurensin g (5), (+)-ampelopsin a (8)) and four stilbenoids (i.e., trans-resveratrol (6), (+)-ampelopsin f (7), piceatannol (9), and trans-piceid (10)). the structures have been identified on the basis of spectroscopic evidence and physicochemical properties. the isolates ... | 2009 | 19280145 |
| inhibition of cholinesterase and amyloid-beta aggregation by resveratrol oligomers from vitis amurensis. | in the course of screening for acetylcholinesterase and butyrylcholinesterase inhibitors from natural products by an in vitro ellman method, the extract of the roots of vitis amurensis rupr. (vitaceae) showed significant cholinesterase inhibitory activity. employing a bioassay-linked hplc method, followed by a semi-preparative hplc method, two compounds of interest were isolated and characterized as vitisin a and heyneanol a. they inhibited effectively both acetylcholinesterase and butyrylcholin ... | 2008 | 18338769 |
| anti-inflammatory tetramers of resveratrol from the roots of vitis amurensis and the conformations of the seven-membered ring in some oligostilbenes. | five resveratrol tetramers, amurensins i-m, were isolated from the roots of vitis amurensis (rupr.), together with five known resveratrol tetramers, (+)-hopeaphenol, isohopeaphenol, vitisin a, (+)-vitisifuran a, and heyneanol a. their structures and stereochemistry were determined by chemical and spectroscopic methods, especially by use of 2d nmr analysis. some of them had an ampelopsin a or a balanocarpol unit, in which the conformations of the seven-membered carbon ring were described for the ... | 2001 | 11551564 |
| oligostilbenes from the roots of vitis amurensis. | two new oligostilbenes, amurensin a (1) and amurensin b (2), were isolated from the roots of vitis amurensis rupr. together with five known oligostilbenes. their structures were elucidated by means of spectroscopic evidence, and amurensin b was the first naturally occurring stilbene trimer with a cis dihydrobenzofuran moiety. | 1999 | 11261202 |
| procyanidins from the seeds of vitis amurensis. | three procyanidins, procyanidin b-5 3'-o-gallate, vitisinol, and amurensisin, were isolated from the seeds of vitis amurensis, whose structures were elucidated on the basis of spectral and chemical evidence. vitisinol and amurensisin contain a spiro-type biflavanyl linkage and a biphenyl-lactone partial structure, respectively. | 2000 | 10820838 |