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proteus penneri isolated from the pus of a patient with epidural abscess.p. mirabilis and p. vulgaris are the two wellknown species in the genus proteus. p. myxofaciens and p. penneri are recent additions to the genus. we isolated p. penneri from the pus of a patient with suppurative otitis media and an epidural abscess. the characteristics of the organism, including morphology, staining, physiology and biochemistry, were studied. clinical microbiological laboratories should suspect p. penneri in the case of as proteus strain that is negative for indole, salicin and ...19921402074
identification of proteus penneri sp. nov., formerly known as proteus vulgaris indole negative or as proteus vulgaris biogroup 1.the name proteus penneri sp. nov. is proposed for a group of organisms previously called proteus vulgaris indole negative or p. vulgaris biogroup 1. all of these strains were salicin negative, esculin negative, and chloramphenicol resistant (zone size, less than 14 mm). dna relatedness studies indicated that when dna from p. penneri strain 1808-73 was labeled and tested against unlabeled dna from 13 other p penneri strains, a highly related group was formed (88 to 99% relatedness at 60 degrees c ...19827050147
comparative in-vitro activity of quinolone carboxylic acids against proteeae.the in-vitro susceptibilities of 198 isolates of precisely identified proteeae species to six quinolone antimicrobials were determined. significant differences in susceptibility patterns among various proteeae to the quinolones examined were demonstrated. although providencia stuartii was found to be the most resistant to quinolones including the very active agent ciprofloxacin, fully speciated prov. rettgeri were also markedly resistant as well. in contrast prov. alcalifaciens was extremely sen ...19846511707
evaluation of rapid one system for identification of 379 strains in the family enterobacteriaceae and oxidase-negative, gram-negative nonfermenters.the ability of the rapid one system (innovative diagnostic systems, inc., norcross, ga.) to identify 364 strains in the family enterobacteriaceae and 15 oxidase-negative, gram-negative, nonfermentative rods was evaluated. kits were inoculated with no. 2 mcfarland standard suspensions, and reactions were interpreted after 4 h of incubation at 35 degrees c. overall, the method correctly identified (to the species level or to the genus level for salmonellas and non-shigella sonnei shigella species) ...19948027345
susceptibilities of 45 clinical isolates of proteus penneri.patterns of susceptibility of 45 proteus penneri clinical isolates to 14 antimicrobial agents were evaluated by a macrobroth dilution method. all strains were highly susceptible to ceftizoxime, ceftazidime, moxalactam, cefoxitin, gentamicin, tobramycin, netilmicin, and, with few exceptions, to amikacin, piperacillin, and cefoperazone. most strains were susceptible to cefotaxime and ceftriaxone. all strains were resistant to cefazolin and cefsulodin.19846508270
hemolytic activity and invasiveness in strains of proteus penneri.twenty strains of proteus penneri obtained from the centers for disease control, atlanta, ga., were tested for their ability to hemolyze sheep and human erythrocytes, a property that is thought to be connected with the invasiveness and virulence of proteus species. in the logarithmic phase of growth, p. penneri cultures are hemolytic for such erythrocytes. this ability is comparable to the hemolysis exhibited by nearly 100% of p. vulgaris and p. mirabilis strains, which is due to the production ...19873597752
structural and immunochemical studies on o-specific polysaccharide of proteus penneri strain 14.the complete structure of the o-antigen of proteus penneri strain 14, containing d-alanine and l-alanine was established using methylation, solvolysis with anhydrous hydrogen fluoride, partial acid hydrolysis, 1h- and 13c-nmr spectroscopy. the role of partial structures of the pentasaccharide repeating unit in manifesting serological specificity and cross-reactivity of this strain with some other bacteria is discussed.19947487355
[urinary tract infections and microbiological characteristics of proteus penneri isolated in taiwan].proteus penneri has been reported to be involved in urinary tract infections and calculi formation. we analyzed 2,265 positive urine cultures from patients hospitalized in taipei veterans general hospital, taiwan and found that 47 patients (2.1%) were infected by p. penneri. most of the patients were male with age over sixty and had been treated with various kinds of antibiotics. most of the patient's urine were collected by catheter. they were alkaline (ph 8.0) and contained amorphous phosphate ...19873652782
comparative in vitro activities of 13 antimicrobial agents against morganella-proteus-providencia group bacteria from urinary tract infections.we examined 741 urinary tract isolates of the morganella-proteus-providencia group, including the recently defined species proteus penneri, for susceptibilities to aminoglycosides, semisynthetic penicillins, and cephalosporins. the data emphasize the importance of identification to the species level on the basis of marked species differences in patterns of susceptibility.19873435110
proteus penneri and urinary calculi formation.the clinical significance of proteus penneri, a newly described species, is unknown. a case report is presented, which is to the best of our knowledge the first description of this organism causing a urinary tract infection and bladder calculi.19846715521
occurrence and pathogenic role of morganella-proteus-providencia group bacteria in human feces.a total of 2,693 fecal specimens, with 1,422 from healthy persons and 1,271 from patients suffering from enteric diseases, was investigated to isolate species of the morganella-proteus-providencia group and to evaluate the role of these bacteria in intestinal disorders. most strains were isolated from two media, i.e., blood agar and tryptophan agar. two of the species were more frequently found in diarrheal cases than in healthy controls. these species were morganella morganii and proteus mirabi ...19863517057
[nosocomial outbreak of proteus penneri infections at a general hospital]. 19892490457
bacteremia and subcutaneous abscess caused by proteus penneri in a neutropenic host.proteus penneri bacteremia and concomitant subcutaneous infection developed in a neutropenic patient with acute lymphocytic leukemia. the skin infection occurred while the patient was being treated empirically with cefoperazone and metronidazole. this case demonstrates the invasive potential of this microorganism in the proper setting.19902380386
[sensitivity to beta-lactam and aminoglycoside antibiotics of clinical proteus strains as dependent upon on their species classification and the source of their isolation].sensitivity of 130 proteus clinical strains was studied. among beta-lactam antibiotics cefotaxime showed marked advantages with respect to various proteus species. all the isolates of proteus mirabilis were sensitive to cefuroxime. cefamezin and cephapirin were inferior by their activity to cefotaxime and cefuroxime. they were characterized by close antibacterial activity and almost complete cross resistance. ampicillin and carbenicillin proved to be the least efficient among the tested beta-lac ...19873326520
cell-free and cell-bound hemolytic activities of proteus penneri determined by different hly determinants.a collection of 45 proteus penneri strains was characterized with respect to their hemolytic activity and representative cell-free or only cell-bound hemolysin possessing strains were chosen for further study. extracellular proteus penneri hemolysin, which was investigated earlier by hybridization, reacted with monospecific antiserum against alpha-hemolysin of escherichia coli. in this paper we also show, using the colony hybridization technique, that the alpha-hemolysin-like determinant is wide ...19911913345
expanded clinical spectrum of infections caused by proteus penneri.strains of proteus penneri from seven abdominal wounds (after bowel resection), five urine samples, and eight other sites were isolated in mixed cultures. seven urine isolates were in pure cultures. all infections were nosocomially acquired, indicating that complete identification of p. penneri in the clinical laboratory is warranted.19873571463
a survey of the aerobic bacteria in the feces of captive raptors.feces of 47 captive raptors belonging to the order falconiformes or strigiformes were cultured for bacteria. gram-negative bacteria, which were cultured from the feces of 45 of the 47 raptors, were the most common isolates. a wide variety of species were identified, including a newly described genus (moellerella wisconsensis), two newly described species (escherichia fergusonii and proteus penneri), and a member of a newly described enteric group (cdc enteric group 41). additional organisms iden ...19883382380
urease activity of proteus penneri.ten strains of proteus penneri isolated from geographically diverse laboratories were tested for urease activity. cell lysates from urea-induced cells had a mean activity of 4.9 +/- 4.1 mumol of nh3 per min per mg of protein. on nondenaturing 6% polyacrylamide activity gels, the enzymes of p. penneri had very similar electrophoretic mobilities within species and within the proteus genus but were distinct from the ureases of providencia and morganella species. on lower-percentage polyacrylamide, ...19873429622
extracellular haemolysin of proteus penneri coded by chromosomal hly genes is similar to the alpha-haemolysin of escherichia coli.extracellular haemolysin of four proteus penneri strains was characterized as a polypeptide of approximately 110 kd. the chromosomal dnas of these strains cleaved with hind iii showed three fragments hybridizing with a dna probe containing cloned haemolysin (hly) genes of escherichia coli. the results presented here suggested that the haemolysin of p. penneri strains is chromosomally determined and similar to the alpha-haemolysin of e. coli.19902205224
the simultaneous production of both hly- and hpm-like hemolysins is characteristic of the proteus penneri species.clinical isolates of proteus penneri were tested for the presence of genes encoding hemolytic activity. strains possessing dna sequences similar to the hlycabd genes in escherichia coli were found. each secreted a 110 kda protein which reacted with a specific anti-hlya antiserum. southern blotting analysis revealed that the hindiii restriction fragment pattern for the hlycabd genes of these strains was conserved. similarly, the chromosomal location of these genes is relatively conserved based on ...19979373950
proteus penneri showing a green colour reaction with kovacs' indole reagent.all 33 investigated strains of proteus penneri gave a green colour reaction with kovacs' indole reagent after an incubation of about three days at 36 degrees c. furthermore, 26% of the 51 strains of proteus mirabilis studied also showed the green colour reaction but somewhat more weakly. the initial compound of this reaction seems to be a product of tryptophan metabolism which is formed only under aerobic conditions.19863526760
the prevalence of gentamicin 2'-n-acetyltransferase in the proteeae and its role in the o-acetylation of peptidoglycan.the prevalence of aac(2')-ia, a gene coding for gentamicin 2'-n-acetyltransferase in providencia stuartii, among species of the proteeae was investigated to determine if it is a common resistance factor and whether the correlation observed in p. stuartii between its expression and the levels of peptidoglycan o-acetylation represents a general feature of bacteria producing this form of modified peptidoglycan. an evaluation of the mics of gentamicin for each of the species of the proteeae did not ...19968961557
lipopolysaccharides of proteus penneri species novum.the lipopolysaccharides (lps), obtained from twenty strains of proteus penneri, were shown to contain 3-deoxy-d-manno-2-octulosonic acid (kdo), l-glycero-d-manno- and d-glycero-d-manno-heptoses, glucose, 2-amino-2-deoxyglucose, 2-amino-2-deoxygalactose, and galacturonic acid. galactose was lacking in three lps and, in some lps, glucuronic acid, rhamnose, lysine, and two unknown constituents were detected. chemotypes of the p. penneri are discussed.19892776126
[properties of a cephalosporinase produced by proteus penneri inhibited by clavulanic acid].p. penneri produces an inducible cephalosporinase, as many enterobacteriaceae. nevertheless this betalactamase is susceptible to clavulanic acid which is an exception also encountered for p. vulgaris. the authors studied the enzyme produced by p. penneri 14hbc resistant to cefotaxime (mic 16 mg/l) isolated in spain in 1992. this betalactamase of isoelectric point 6.65 hydrolyzes first generation cephalosporins, amoxycillin and poorly ticarcillin as it occurs for all cephalosporinases. however, t ...19947824319
the structure of proteus penneri strain 14-o-specific polysaccharide containing d- and l-alanine. 19911725142
2-acetamido-4-o-[(s)-1-carboxyethyl]-2-deoxy-d-glucose: a new natural isomer of n-acetylmuramic acid from the o-specific polysaccharide of proteus penneri 35. 19947518741
structure of the o-specific polysaccharide of proteus penneri 62 containing 2-acetamido-3-o-[(s)-1-carboxyethyl]-2-deoxy-d-glucose (n-acetylisomuramic acid). 19921473100
serological characterization of proteus penneri species novum.serological characterization of the first collection of the 20 proteus penneri strains is presented. all anti-0 sera were examined in microagglutination, semi-quantitative precipitation and passive hemagglutination tests. some p. penneri lipopolysaccharides showed strong cross-reactivity in passive hemagglutination additionally confirmed by inhibition in this test. serological similarity between species within genus proteus is discussed.19921485834
identification and typing of proteus penneri and proteus vulgaris biogroups 2 and 3, from clinical sources, by computerized analysis of electrophoretic protein patterns.seventy-six strains of the proteus vulgaris complex (pr. penneri and pr. vulgaris biogroups 2 and 3) were characterized by one-dimensional sds-page of cellular proteins. the protein patterns were highly reproducible. the strains came from various countries and were mainly of human origin: urine (28), respiratory tract (13), wounds (8), faeces (7), blood (3), miscellaneous sources (6) and unknown sources (11). the patterns of these strains, together with those of the type strains of seven morgane ...19938300450
replacement of nctc 4175, the current type strain of proteus vulgaris, with atcc 29905. request for an opinion.the current type strain of proteus vulgaris, nctc 4175 (= atcc 13315), differs substantially from typical strains of this species both biochemically and chemotaxonomically. dna relatedness studies revealed that strains previously classified as p. vulgaris belong to six genomospecies. one of these genomospecies contains strains that are negative in indole, salicin, and esculin reactions (biogroup 1) and has been named proteus penneri. a second genomospecies, which is most frequently isolated from ...19957547312
[immunochemical studies of o-specific polysaccharide from proteus penneri 14 lipopolysaccharide].o-specific polysaccharide was obtained on mild acid degradation of proteus penneri strain 14 lipopolysaccharide and found to contain equimolar of d-galactose, d-ribose, 2-acetamido-2-deoxy-d-glucose, n-(d-galacturonoyl)-l-alanine and 3-(nacety-l-alanyl)-amido-3,6-dideoxy-d-glucose. on the basis of non-destructive nmr analysis it was concluded that repeat unit of the 0-specific polysaccharide of p. penneri 14 has the following structure: -2-beta-d- quip3nalaac-(1-->4)-alfa-d-galpaala-(1-->2)-beta ...19938231451
the structure of the o-specific polysaccharide chain of proteus penneri strain 42 lipopolysaccharide. 19957585721
the activity of cefpirome and ten other antibacterial agents against 2858 clinical isolates collected from 20 centres.two thousand eight hundred and fifty-eight aerobic clinical isolates of enterobacteriaceae, pseudomonas species, staphylococci, streptococci and haemophilus species were collected in 19 geographically separated centres in the uk and one in ireland. the identity of each isolate was confirmed at southmead hospital and the mic of cefpirome, cefotaxime, ceftazidime, cefuroxime, cephradine, amoxycillin, piperacillin, imipenem, gentamicin, ciprofloxacin and trimethoprim was determined by an agar dilut ...19938486569
[characterization of hemolytic activity of proteus penneri].bacteria belonging to the genus proteus synthesise two kinds of hemolysins hpma and hlya which represent "rtx proteins". in previous papers we described the production of an extracellular hlya hemolysin by some p. penneri strains. now we are reporting on the synthesis by p. penneri, typical for p. mirabilis hpma hemolysin. there were identified two p. penneri strains 5 and 37 in which both hpma and hlya regions are present. in two other strains p. penneri 13 and 44 only hlya region was found, wh ...19938231450
[immunochemical studies of o-specific polysaccharide of proteus penneri 42 lipopolysaccharide].o-specific polysaccharide was obtained an mild acid degradation of proteus penneri strain 42 lipopolysaccharide and found to contain d-glucose, d-galacturonic acid and 2-acetamido-2-deoxy-d-glucose in molar ratio 2:1:1. methylation analysis showed that the polysaccharide is linear, one of the glucose residue is substituted at position 2, the second one and the residue of galacturonic acid at position 4, and the 2-acetamido-2-deoxy-d-glucose residue at position 3. on the basis of non-destructive ...19938231452
in vitro activities of antimicrobial agents against proteus species from clinical specimens.two hundred clinical isolates of members of the genus proteus were definitively identified and their antimicrobial susceptibilities to 12 antimicrobials tested, 176 isolates (88%) being identified as proteus mirabilis, 12 strains (6%) as proteus vulgaris and 12 strains (6%) as proteus penneri. most strains were isolated from pus (62.5%) and urine (34%), but in general there were no significant differences in the rates of isolation of any of the species by age or sex, although it was noted that p ...19948049615
structure of the o-specific polysaccharide chain and serological characterization of the proteus penneri 62 lipopolysaccharide compared with the lipopolysaccharides of the p. penneri strains.the chemical structure of the o-specific polysaccharide chain of proteus penneri 62 lipopolysaccharide (lps) containing n-acetylisomuramic acid was established using acid hydrolysis, solvolysis with anhydrous hydrogen fluoride and 1h and 13c nmr spectroscopy. cross reactivity of the anti-o-serum p. penneri 62 with a number of other strains of the same species isolated in the usa, canada, germany and poland is discussed.19968915524
structural and immunochemical studies on the lipopolysaccharide of the 't-antigen'-containing mutant proteus mirabilis r14/1959.in doc-page, lipopolysaccharide (lps) of proteus mirabilis r14/1959 (rb-type) mutant showed a ladder-like migration pattern indicating the presence of a high molecular weight polysaccharide chain. the isolated polysaccharide, called t-antigen because of similarity with the t1 chain of salmonella friedenau lps, contained d-glucose, d-galacturonic acid (d-gala), and d-glcnac in molar ratios 2:1:1 and was structurally different from the o-antigen of the parental s-strain p. mirabilis s1959 but iden ...19968731019
structure of a new n-acetylisomuramic acid-containing o-specific polysaccharide of proteus penneri strains 19 and 35.o-specific polysaccharides, together with oligosaccharide products of their degradation, were isolated by gpc after mild acid delipidation of lipopolysaccharides of proteus penneri strains 19 and 35. the polysaccharides had the same trisaccharide repeating unit containing one residue each of d-galactose, 2-acetamido-2-deoxy-d-glucose, and 2-acetamido-3-o-[(s)-1-carboxyethyl]-2-deoxy-d-glucose (n-acetylisomuramic acid). on the basis of 1d and 2d 1h and 13c nmr spectroscopy, including 2d correlati ...19968916545
new serogroups of the genus proteus consisting of proteus penneri strains only. determination of some lps epitopes responsible for specificity. 200011109127
structure of the o-specific polysaccharide of proteus penneri strain 25 containing n-(l-alanyl) and multiple o-acetyl groups in a tetrasaccharide repeating unit.based on sugar and methylation analyses, o-deacetylation, smith degradation, and 1h and 13c nmr spectroscopy, including 2d cosy, 1h-detected 1h, 13c heteronuclear single-quantum coherence (hsqc), and 1h-detected 1h, 13c heteronuclear multiple-bond connectivity (hmbc) experiments, the following structure of the o-specific polysaccharide of proteus penneri strain 25 was established: [formula: see text] where d-glcn(l-ala) is 2-(l-alanylamido)-2-deoxy-d-glucose.19979090817
acute pyelonephritis in a child caused by proteus penneri. 19979227829
analysis of antibiotic resistance determinants in proteus penneri.the plasmid profiles of 65 strains of proteus penneri were analyzed to determine whether resistance was determined chromosomally or by plasmids. only seven strains harboured one to three plasmids, although these strains exhibited resistance to a wide range of antibiotics. markers for ampicillin and tetracycline resistance could be transferred to escherichia coli by transformation. plasmids carried resistance to chloramphenicol in two strains and resistance to sulfonamides in one strain. the resu ...19938359170
purification and properties of a beta-lactamase from proteus penneri.a cephalosporin-hydrolyzing enzyme from strains of proteus penneri resistant to beta-lactam antibiotics was purified and characterized. the enzyme gave a single protein band on sds-polyacrylamide gel electrophoresis with a molecular weight of 30,000. this cephalosporinase has an isoelectric point of 6.8, a ph optimum of 6.5 and a temperature optimum of 45 degrees c. the enzyme hydrolyzed cephaloridine, cephalothin, cefuroxime, and cefotaxime more rapidly than penicillins. the relative rate, with ...19863489701
structure and cross-reactivity of the o-specific polysaccharide of proteus penneri strain 26, another neutral proteus o-antigen containing 2-acetamido-2,6-dideoxy-l-glucose (n-acetyl-l-quinovosamine).a neutral o-specific polysaccharide obtained from the lipopolysaccharide of proteus penneri strain 26 was studied using sugar analysis and 1h and 13c nmr spectroscopy, including two-dimensional nmr techniques. the following structure of the trisaccharide repeating unit was established: -->6)-alpha-d-glcpnac-(1-->3)-alpha-l-quipnac-(1-->3)-alpha-d-glcp nac-(1--> where l-quinac is 2-acetamido-2,6-dideoxy-l-glucose (n-acetyl-l-quinovosamine). cross-reactivity of the proteus penneri 26 anti-o serum ...19989654072
the production of hlya toxin by proteus penneri strains.twelve diverse strains of proteus penneri of clinical origin all produced a calcium-dependent haemolysin, unlike most other proteus spp. in most strains the haemolysin was secreted into the medium during early exponential growth and lysed not only of a variety of erythrocyte types from several animals including man, but also human neutrophils and human embryo lung fibroblasts. the haemolysin was a protein of 107 kda, the same size as escherichia coli hlya, and it reacted with antiserum to e. col ...19938411089
the structure of the o-specific polysaccharide chain of proteus penneri strain 16 lipopolysaccharide.o-specific polysaccharide was obtained by mild acid degradation of proteus penneri strain 16 lipopolysaccharide and found to contain d-glucose, d-glucuronic acid, 2-acetamido-2-deoxy-d-glucose, and 3,6-dideoxy-3-[(r)-3-hydroxybutyramido]- d-galactose in the ratio of 2:1:1:1 as well as a small proportion of o-acetyl groups. on the basis of one-dimensional 1h-nmr13c-nmr and noe spectroscopy, two-dimensional homonuclear-shift-correlated spectroscopy with one-step and two-step relayed coherence tran ...19912015828
the haemagglutinins and fimbriae of proteus penneri.the haemagglutinins and fimbriae produced by 18 strains of proteus penneri were studied and compared with those formed by representative strains of other species of proteeae. after repeated subcultures at 30 degrees c, 12 p. penneri strains formed only mr/k haemagglutinins which were associated with thin, non-channelled, type-3 fimbriae. two strains formed simultaneously both ms and mr/k haemagglutinins associated with thick, channelled, type-1 fimbriae and type-3 fimbriae, respectively. four st ...19892574748
[selected properties of strains of a new species of proteus penneri from the second american collection].the second collection of the novel species proteus penneri consists of 25 strains from which only two have shown rough from properties in the tests differentiating s and r variants of bacteria. the migration pattern of their lipopolysaccharides in gel electrophoresis was leader-like, typical for smooth organisms. 13 out of 25 lipopolysaccharide preparations showed strong-reactivity with anti-0 sera in semi-quantitative precipitation test. serological similarity between the strains within species ...19938231447
[isolation and certain biological and immunologic properties of proteus penneri strains from the european collection].the american collection of 45 proteus penneri strains was supplemented by 22 strains isolated in poland and germany. all strains exhibited typical smooth forms of bacteria. susceptibility to antibiotics was determined and compared with the other p. penneri strains. 0-antigenic relatedness and serological similarity between strains in the whole proteus penneri collection were discussed.19938231449
genetic diversity in proteus penneri.dna of thirteen proteus penneri strains derived from four european countries (nine strains from germany, two strains from united kingdom, one strain from turkey, one strain from hungary) was examined by random amplified polymorphic dna-pcr (rapd-pcr) method. rapd with primer aacgcgcaac gave different patterns, which suggests a dna sequence microdiversity within this species. the method provides a fast, economical and reproducible means for typing p. penneri.19979847452
the structure of the o-specific polysaccharide of proteus penneri 52.an acidic o-specific polysaccharide isolated from proteus penneri 52 contains d-galacturonic acid, 2-acetamido-2-deoxy-d-glucose, and 2-acetamido-2-deoxy-d-galactose in the ratio 1:2:2. on the basis of sugar analysis and nmr spectroscopy, which included one-dimensional tocsy, two-dimensional cosy, heteronuclear 13c, 1h-cosy, and rotating-frame noe spectroscopy, the following structure of the pentasaccharide repeating unit of the o-specific polysaccharide was established: [sequence: see text] cro ...19968797860
structure of the o-specific polysaccharide of a serologically separate strain proteus penneri 2 from a new proposed serogroup o66.o-specific polysaccharide chain of proteus penneri strain 2 lipopolysaccharide was studied by full and partial acid hydrolysis, smith degradation, methylation analysis, and nmr spectroscopy, including two-dimensional rotating-frame noe spectroscopy (roesy) and 1h,13c heteronuclear multiple-quantum coherence (hmqc) experiments. together with d-glucose and 2-acetamido-2-deoxy-d-glucose, the polysaccharide was found to contain two rarely occurring sugars, 6-deoxy-l-talose (l-6dtal) and 2,3-diacetam ...199910215848
structure and epitope specificity of the o-specific polysaccharide of proteus penneri strain 12 (atcc 33519) containing the amide of d-galacturonic acid with l-threonine.o-specific polysaccharide was isolated from proteus penneri strain 12 (atcc 33519) lipopolysaccharide (lps) and studied using nmr spectroscopy, including selective spin-decoupling, one-dimensional noe, two-dimensional homonuclear correlation spectroscopy, 13c,1h heteronuclear correlation spectroscopy and chemical methods (o-deacetylation, smith degradation, partial acid hydrolysis followed by borohydride reduction and methylation). the amide of d-galacturonic acid with l-threonine [d-gala(l-thr) ...19957541754
proteus penneri urosepsis in a patient with diabetes mellitus.proteus penneri has been isolated from many different clinical sources, including surgical wound infections, urine, and blood. we describe the first reported case of p. penneri nosocomial urosepsis in a patient with diabetes. p. penneri was subsequently isolated from bronchoalveolar lavage fluid and a pulmonary artery catheter tip.19989548071
molecular characterization of the genera proteus, morganella, and providencia by ribotyping.the so-called proteus-providencia group is constituted at present by three genera and 10 species. several of the recognized species are common opportunistic pathogens for humans and animals. different methods based on the study of phenotypic characters have been used in the past with variable levels of efficiency for typing some species for epidemiological purposes. we have determined the rrna gene restriction patterns (ribotypes) for the type strains of the 10 different species of the genera pr ...199910449462
diversity among clinical isolates of proteus penneri detected by random amplified polymorphic dna analysis.dna of thirteen haemolytic proteus penneri strains of clinical origin, all producing calcium dependent haemolysin and having been derived from four european countries was examined for plasmid profile, and outer membrane protein profile, by random amplified polymorphic dna-pcr (rapd-pcr) method, and digestions with restriction endonucleases were performed. all strains contained two large plasmids of approximately 60 and 70 kilobase pairs (kb). in addition, four strains contained a small plasmid o ...19989861679
structure of the o-specific polysaccharide of a serologically separate proteus penneri strain 22.the o-specific polysaccharide chain (o-antigen) of proteus penneri strain 22 lipopolysaccharide was studied using chemical methods, including partial acid hydrolysis and smith degradation, as well as one- and two-dimensional 1h and 13c nmr spectroscopy. the following structure of the pentasaccharide repeating unit was established: [sequence: see text] the o-specific polysaccharide contains a galnac residue in the furanose form which has not been hitherto found in bacterial polysaccharides. the o ...19989794073
structure of the o-specific polysaccharide of proteus penneri strain 41 from a new proposed serogroup o62.o-specific polysaccharide of proteus penneri strain 41 was studied using 1h- and 13c-nmr spectroscopy, including two-dimensional cosy, heteronuclear 13c,1h-correlation (hetcor) and one-dimensional noe spectroscopy, and the following structure of a non-stoichiometrically o-acetylated hexasaccharide repeating unit was established:[structure: see text] where rglcnac is 2-acetamido-4-o-[(s)-1-carboxyethyl]-2-deoxyglucose. cross-reactivity of anti-p. penneri 41 o-serum with other p. penneri strains i ...19989657315
effect of spontaneous and induced mutations on outer membrane proteins and lipopolysaccharides of proteus penneri strain 357. 200011109104
[enzymatic resistance to beta lactam antibiotics within the genus proteus and evaluation of proteus mirabilis phenotypes and genotypes for resistance to third- and fourth-generation cephalosporins].the aim of this study was to evaluate betalactam resistance within the genus proteus and characterize the betalactamases responsible for this resistance.200515757582
determination of genetic diversity of proteus penneri strains using rep-pcr. 200011109122
use of randomly amplified polymorphic dna (rapd) analysis for identification of proteus penneri. 200011109123
the structure of the carbohydrate backbone of the core-lipid a region of the lipopolysaccharide from proteus penneri strain 40: new proteus strains containing open-chain acetal-linked n-acetylgalactosamine in the core part of the lps.analysis of the core part of the lps from several strains of proteus revealed that p. penneri strains 2, 11, 19, 107, and p. vulgaris serotypes 04 and 08 have the same structure with a new type of linkage between monosaccharidesan open-chain acetal--that was previously determined for p. vulgaris ox2 and p. penneri 17. the lps from p. penneri strain 40 contains the same structure substituted with one additional monosaccharide: [molecular structure: see text] where (1s)-galanac1 is a residue of n- ...200111269407
structure of a new neutral o-specific polysaccharide of proteus penneri 34.the o-specific polysaccharide of proteus penneri strain 34 was studied using 1h and 13c nmr spectroscopy, including 2d cosy, tocsy, noesy, and h-detected 1h, 13c hmqc experiments. the following structure was established, which is unique among the known structures of proteus o-antigens:-->4)-beta-d-glcp-(1-->3)-beta-d-galpnac-(1-->4)-beta- d-galpnac-(1-->4)-beta-d-galp-(1-->. accordingly, no cross-reaction was observed between p. penneri 34 o-antiserum and o-antigens of other proteus strains. the ...19989836454
classification of proteus vulgaris biogroup 3 with recognition of proteus hauseri sp. nov., nom. rev. and unnamed proteus genomospecies 4, 5 and 6.strains traditionally identified as proteus vulgaris formed three biogroups. biogroup 1, characterized by negative reactions for indole production, salicin fermentation and aesculin hydrolysis, is now known as proteus penneri. biogroup 2, characterized by positive reactions for indole, salicin and aesculin, was shown by dna hybridization (hydroxyapatite method) to be a genetic species separate from biogroup 1 and from biogroup 3 which is positive for indole production and negative for salicin an ...200011034498
structure of a 2-aminoethyl phosphate-containing o-specific polysaccharide of proteus penneri 8 from a new serogroup o67.an acidic o-specific polysaccharide was obtained by mild acid degradation of the proteus penneri 8 lipopolysaccharide and found to contain d-glucose, d-galacturonic acid, 2-acetamido-2-deoxy-d-glucose, 2-acetamido-2-deoxy-d-galactose, 2-acetamido-2,6-dideoxy-l-galactose (l-fucnac) and 2-aminoethyl phosphate (petn) in the ratios 2 : 1 : 1 : 1 : 1 : 1. 1h and 13c nmr spectroscopy was applied to the intact and dephosphorylated polysaccharides, and the following structure of the hexasaccharide repea ...200010651819
the structure of the carbohydrate backbone of the rough type lipopolysaccharides from proteus penneri strains 12, 13, 37 and 44.the following structure of the lipid a-core backbone of the rough type lipopolysaccharides (lps) from proteus penneri strains 12, 13, 37, and 44 was determined using nmr and mass spectroscopy and chemical analysis of the oligosaccharides obtained by mild-acid hydrolysis, alkaline o,n-deacylation, o-deacylation with hydrazine, and deamination of the lpss:where k=h, r=petn, r(1)=alpha-hep-(1-->2)-alpha-ddhep, and r(2)=alpha-galn (strains 12 and 13) or beta-glcnac-(1-->4)-alpha-glcn (strains 37 and ...200211996837
the structure of the core part of proteus penneri strain 16 lipopolysaccharide.the structure of the carbohydrate backbone of the lipid a-core region of the lipopolysaccharide (lps) from proteus penneri strain 16 was determined using nmr and chemical analysis of the core oligosaccharide, obtained by mild acid hydrolysis of the lps, and of the products of alkaline deacylation of the lps: formula [see text]. incomplete substitution is indicated by bold italics. all sugars are in the pyranose form, alpha-hep is the residue of l-glycero-alpha-d-manno-hep, alpha-dd-hep is the re ...200010903028
structure of the o-specific polysaccharide of proteus penneri 71 and classification of cross-reactive p. penneri strains to a new proposed serogroup o64.a neutral o-specific polysaccharide (o-antigen) was isolated from the lipopolysaccharide (lps) of the bacterium proteus penneri 71. on the basis of sugar analysis and 1h- and 13c-nmr spectroscopic studies, including two-dimensional cosy, 13c,1h heteronuclear cosy and roesy, the following structure of the trisaccharide repeating unit of the polysaccharide was established: -->3)-beta-d-glcpnac-(1-->4)-beta-d-glcpnac-(1-->3)-alpha-d-galp-(1-- > the polysaccharide has the same carbohydrate backbone ...200010651818
new structures of the o-specific polysaccharides of proteus. 2. polysaccharides containing o-acetyl groups.structures of five new o-specific polysaccharides of proteus bacteria were established. four of them, proteus penneri 4 (o72), proteus vulgaris 63/57 (o37), proteus mirabilis tg 277 (o69), and proteus penneri 20 (o17), contain o-acetyl groups in non-stoichiometric quantities, and the polysaccharide of p. penneri 1 is structurally related to that of p. penneri 4. the structures were elucidated using nmr spectroscopy, including one-dimensional 1h- and 13c-nmr spectroscopy, two-dimensional 1h,1h co ...200211952416
structure of the o-specific polysaccharide of proteus penneri 103 containing ribitol and 2-aminoethanol phosphates.the o-specific polysaccharide of the lipopolysaccharide of proteus penneri strain 103 was studied using 1h and 13c nmr spectroscopy, including 2d cosy, tocsy, noesy, h-detected 1h,(13)c hmqc, 1h, 31p hmqc, and hmbc experiments. it was found that the polysaccharide is built up of oligosaccharide-ribitol phosphate repeating units and thus resembles ribitol teichoic acids of gram-positive bacteria. the following structure of the polysaccharide was established:where etn and rib-ol are ethanolamine a ...200212350322
identification of a chromosome-borne expanded-spectrum class a beta-lactamase from erwinia persicina.from whole-cell dna of an enterobacterial erwinia persicina reference strain that displayed a penicillinase-related antibiotic-resistant phenotype, a beta-lactamase gene was cloned and expressed in escherichia coli. it encoded a clavulanic-acid-inhibited ambler class a beta-lactamase, erp-1, with a pi value of 8.1 and a relative molecular mass of ca. 28 kda. erp-1 shared 45 to 50% amino acid identity with the most closely related enzymes, the chromosomally encoded enzymes from citrobacter koseri ...200212384342
structural and immunochemical studies on the o-specific polysaccharide of proteus penneri strain 15.on the basis of sugar analysis and 1h- and 13c-nmr spectroscopy, it was shown that the o-specific polysaccharide of proteus penneri strain 15 has a trisaccharide repeating unit, including an acetal-linked pyruvic acid residue, and is structurally identical to the capsular polysaccharide of proteus vulgaris strain atcc 49990. serological studies supported this conclusion and demonstrated the presence in the homological antiserum of both anti-core and anti-o chain antibodies reacting with a lipopo ...19979437499
[investigation of hydrophobicity of proteus vulgaris strains and ability of proteus vulgaris and proteus penneri strains to penetrate bladder membrane hcv t-29 cells ].proteus bacilli play a particularly important role in urinary tract infections (uti). fimbriae and adherence ability and hemolysins production (hpma, hlya) are one of the factors of pathogenicity of these bacteria. in this paper we describe the invasion of hcv t-29 transitional bladder urothelial cells carcinoma strains of p. penneri, as well as p. vulgaris strains belonging to different serogroups. the cytotoxic effect was observed at 8 hour of incubation of the tested cells with p. vulgaris o2 ...200212650056
capillary electrophoretic analysis of wild type and mutant proteus penneri outer membrane proteins.in this study the virulence factors, outer membrane proteins (omp), lipopolysaccharides (lps), hemolysin, and the in vivo and in vitro virulence of wild-type proteus penneri 357 and its two isogenic mutant variants--a transposon and a spontaneous mutant--were examined. the omps of these variants were analyzed by a new and fast technique, "dynamic sieving" capillary electrophoresis (ce). the omp profiles were dominated by two peaks (39 and 43 kda). in the p. penneri clone examined, both the trans ...200011001319
structure of the o-polysaccharide of a serologically separate strain of proteus mirabilis, tg 332, from a new proposed proteus serogroup o50.the o-polysaccharide was obtained by mild acid degradation of the lipopolysaccharide of proteus mirabilis tg 332 strain. the following structure of the o-polysaccharide was determined by chemical methods along with nmr spectroscopy, including 2d cosy, tocsy, roesy and 1h, 13c hmqc experiments: [see equation in text]. the o-polysaccharide studied has a unique structure among proteus o-antigens. accordingly, p. mirabilis tg 332 is serologically separate, and we propose to classify this strain into ...200314505878
natural antibiotic susceptibility of proteus spp., with special reference to p. mirabilis and p. penneri strains.the natural susceptibility of 102 proteus mirabilis and 35 proteus penneri strains to 71 antibiotics was examined. minimum inhibitory concentrations (mics) were determined by applying a microdilution procedure in isosensitest broth (for all strains) and cation-adjusted mueller hinton broth (for some strains). p. mirabilis and p. penneri were naturally resistant to penicillin g, oxacillin, all tested macrolides, lincosamides, streptogramins, glycopeptides, rifampicin and fusidic acid. both specie ...200312678409
new structures of the o-specific polysaccharides of proteus. 3. polysaccharides containing non-carbohydrate organic acids.four new proteus o-specific polysaccharides were isolated by mild acid degradation from the lipopolysaccharides of p. penneri 28 (1), p. vulgaris o44 (2), p. mirabilis g1 (o3) (3), and p. myxofaciens (4), and their structures were elucidated using nmr spectroscopy and chemical methods. they were found to contain non-carbohydrate organic acids, including ether-linked lactic acid and amide-linked amino acids, and the following structures of the repeating units were established: [figure: see text], ...200312765528
crystal structure of a cyclic enterobacterial common antigen. 200312800183
structure of the o-polysaccharide of proteus penneri 28 and proteus vulgaris o31 and classification of p. penneri 26 and 28 in proteus serogroup o31.the lipopolysaccharides (lps) of proteus penneri 28 and proteus vulgaris o31 (prk 55/57) were degraded with dilute acetic acid and structurally identical high-molecular-mass o-polysaccharides were isolated by gel-permeation chromatography. sugar analysis and nuclear magnetic resonance (nmr) spectroscopic studies showed that both polysaccharides contain d-glcnac, 2-acetamido-2,6-dideoxy-l-glucose (l-2-acetamido-2,6-dideoxyglucose (n-acetylquinovosamine)) and 2-acetamido-3-o-[(s)-1-carboxyethyl]-2 ...200314557001
structure and serological studies of the o-polysaccharide of proteus penneri 75 epitopes and subgroups of proteus serogroup o73.the o-specific polysaccharide of the lipopolysaccharide of proteus penneri strain 75 consists of tetrasaccharide-ribitol phosphate repeating units and resembles ribitol teichoic acids of gram-positive bacteria. the following structure of the polysaccharide was elucidated by chemical methods and 1h and 13c nmr spectroscopy: [structure in text] where rib-ol is ribitol. serological studies with polyclonal antisera showed that the same structure of the o-polysaccharide occurred in two strains: p. pe ...200515681143
structural and serological characterization of the lipopolysaccharide from proteus penneri 20 and classification of the cross-reacting proteus penneri strains 10, 16, 18, 20, 32 and 45 in proteus serogroup o17.o-specific polysaccharide (o-antigen) of the lipopolysaccharide of proteus penneri 20 was studied using sugar analysis along with various one- and two-dimensional nmr spectroscopy techniques. the following structure of the polysaccharide was established: [formula: see text] it has the same carbohydrate backbone structure as that described earlier for p. penneri 16, in which the positions of the o-acetyl groups have not been determined. p. penneri 20 o-antiserum showed a strong cross-reactivity w ...200212455869
structure of the o-polysaccharide leads to classification of proteus penneri 31 in proteus serogroup o19.o-polysaccharide was obtained by mild acid degradation of the lipopolysaccharide (lps) of proteus penneri strain 31. sugar and methylation analyses along with nmr spectroscopic studies, including 2d 1h,1h cosy, tocsy, roesy, 1h,13c and 1h,31p hmqc experiments, demonstrated the following structure of the polysaccharide: [carbohydrate structure: see text] where fucnac is 2-acetamido-2,6-dideoxygalactose and etnp is 2-aminoethyl phosphate. the polysaccharide studied has the same carbohydrate backbo ...200314556999
structural and serological studies of the o-antigen of proteus mirabilis o-9.the following structure of the o-polysaccharide (o-antigen) of the lipopolysaccharide of proteus mirabilis o-9 was determined by nmr spectroscopy, including 2d 1h,(1)h cosy, tocsy, roesy, and 1h,(13)c hmqc experiments, along with chemical methods: [chemical structure: see text] where the degree of o-acetylation is approximately 70%. immunochemical studies using rabbit polyclonal anti-proteus mirabilis o-9 serum showed the importance of the o-acetyl groups in manifesting the serological specifici ...200312747861
structure of the o-polysaccharide and serological studies of the lipopolysaccharide of proteus penneri 60 classified into a new proteus serogroup o70.an alkali-treated lipopolysaccharide of proteus penneri strain 60 was studied by chemical analyses and 1h, 13c and 31p nmr spectroscopy, and the following structure of the linear pentasaccharide-phosphate repeating unit of the o-polysaccharide was established: 6)-alpha-d-galp-(1-->3)-alpha-l-fucpnac-(1-->3)-alpha-d-glcpnac-(1-->3)-beta-d-quip4nac-(1-->6)-alpha-d-glcp-1-p-(o--> rabbit polyclonal o-antiserum against p. penneri 60 reacted with both core and o-polysaccharide moieties of the homologo ...200515708308
serological classification and epitope specificity of proteus penneri s29 lipopolysaccharide.gram-negative bacteria of genus proteus are common human intestinal and urinary tract pathogens. in the genus proteus there are four clinically important named species: p. mirabilis, p. vulgaris, p. penneri, and p. hauseri, and three unnamed proteus genomospecies: 4, 5, and 6. the clinical significance of p. penneri, described in 1982 as a new species, is poorly documented. the aim of this work is serological characterization and classification of a ceftriaxone-susceptible p. penneri s29 strain ...200516407787
evaluation of the phoenix 100 id/ast system and nid panel for identification of enterobacteriaceae, vibrionaceae, and commonly isolated nonenteric gram-negative bacilli.the phoenix 100 id/ast system (becton dickinson co., sparks, md.) is an automated system for the identification and antimicrobial susceptibility testing of bacterial isolates. this system with its negative identification (nid) panel was evaluated for its accuracy in the identification of 507 isolates of the family enterobacteriaceae, 57 other nonenteric gram-negative isolates that are commonly isolated in clinical microbiology laboratories, and 138 isolates of the family vibrionaceae. all of the ...200616517878
postneurosurgical meningitis due to proteus penneri with selection of a ceftriaxone-resistant isolate: analysis of chromosomal class a beta-lactamase huga and its lysr-type regulatory protein hugr.we report on a case of a postneurosurgical meningitis due to ceftriaxone-susceptible proteus penneri, with selection of a ceftriaxone-resistant isolate following treatment with ceftriaxone. the isolates presented identical patterns by pulsed-field gel electrophoresis and produced a single beta-lactamase named huga with an isoelectric point of 6.7. the ceftriaxone-resistant isolate hyperproduced the beta-lactamase (increase in the level of production, about 90-fold). the sequences of the huga bet ...200211751137
application of two different kinds of sera against the proteus penneri lipopolysaccharide core region in search of epitopes determining cross-reactions with antibodies.proteus penneri lipopolysaccharide (lps) core regions are characterized by a greater structural variability than that observed in other enterobacteriaceae. this fact and the small amount of published data concerning the serological activity of this part of p. penneri lps prompted an examination of which fragment might determine cross-reactions with antibodies. to date, such epitopes have been found in the lps core regions of p. mirabilis and p. vulgaris strains.200818373243
characterization and serological classification of a collection of proteus penneri clinical strains.bacteria of the genus proteus, which are a common cause of urinary tract infections, are divided into four species: p. mirabilis, p. vulgaris, p. penneri, and p. hauseri, and three unnamed genomospecies, proteus 4, 5, and 6 (single-strain species p. myxofaciens was isolated from the gypsy moth). establishing the serological classification of these species would aid in completing the classification scheme of the whole genus proteus and in applying serological methods in diagnostic procedures and ...200415179326
structure of a 2-aminoethyl phosphate-containing o-specific polysaccharide of proteus penneri 63 from a new serogroup o68.lipopolysaccharide of proteus penneri strain 63 was degraded by mild acid to give a high molecular mass o-specific polysaccharide that was isolated by gel-permeation chromatography. sugar and methylation analyses and nmr spectroscopic studies, including two-dimensional 1h, 1h cosy, tocsy rotating-frame noe spectroscopy, h-detected 1h,13c and 1h,31p heteronuclear multiple-quantum coherence (hmqc), and 1h, 13c hmqc-tocsy experiments, demonstrated the following structure of the polysaccharide: wher ...200010632731
structure of the core part of the lipopolysaccharides from proteus penneri strains 7, 8, 14, 15, and 21.the core-lipid a region of the lipopolysaccharides from proteus penneri strains 7, 8, 14, 15, and 21 was studied using nmr spectroscopy, esi ms, and chemical analysis after alkaline deacylation, deamination, and mild-acid hydrolysis of the lipopolysaccharides. the following general structure of the major core oligosaccharides is proposed: [abstract: see text] where all sugars are in the pyranose form and have the d configuration unless otherwise stated, hep and ddhep=l-glycero- and d-glycero-d-m ...200211909598
structure and gene cluster of the o-antigen of escherichia coli o109; chemical and genetic evidences of the presence of l-rhan3n derivatives in the o-antigens of e. coli o109 and o119.o-antigen representing the o-polysaccharide chain of the lipopolysaccharide is the most variable constituent on the cell surface of gram-negative bacteria and a player in their pathogenicity. the o-polysaccharide of escherichia coli o109 was studied by sugar analysis and nuclear magnetic resonance spectroscopy and found to contain a rarely occurring monosaccharide, 2,3-diacetamido-2,3,6-trideoxy-l-mannose (l-rhanac3nac). the following structure of the tetrasaccharide repeating unit of the o-poly ...201020964722
structure and serological properties of the o-antigen of two clinical proteus mirabilis strains classified into a new proteus o77 serogroup.two proteus mirabilis strains, 3 b-m and 3 b-k, were isolated from urine and faeces of a hospitalized patient from lodz, poland. it was suggested that one strain originated from the other, and the presence of the bacilli in the patient's urinary tract was most probably a consequence of autoinfection. the o-polysaccharide was obtained by mild acid degradation of the lipopolysaccharide of p. mirabilis 3 b-m and studied by sugar analysis and nuclear magnetic resonance spectroscopy, including two-di ...200818665848
biogenic amines produced by enterobacteriaceae isolated from meat products.biogenic amines in ground meat and processed meat products are one of the indicators to determine the poor quality raw material. major histamine forming bacterium was escherichia coli (strain ec04 with 65.88 mg/100 ml in brain heart infusion medium), followed by the microorganisms morganella morganii (strain mm4 with 8.40 mg/100 ml and strain mm7 with 8.28 mg/100 ml) and proteus mirabilis (strain pm02 with 8.76 mg/100 ml), respectively. the highest putrescine production level was found in citrob ...200122062111
antibiotic susceptibility profiles of uncommon bacterial species causing severe infections in italy.this study presents the results of the italian "severe infections project" involving bacteria that can be considered rare causes of disease. we isolated 30 uncommon human pathogens from a total of 60 strains (1.2% of all the isolates). the most frequent sources of uncommon human pathogens were primary bloodstream infections (48.3%) and pneumonia (20%). species such as comamonas testosteroni, enterococcus hirae, kluyvera ascorbata, kluyvera cryocrescens, leclercia adecarboxylata and ochrobactrum ...200919567344
draft genome sequence of the bioelectricity-generating and dye-decolorizing bacterium proteus hauseri strain zmd44.proteus hauseri zmd44 (cgmcc 6746), as a crucial biodecolorizing, bioelectricity-generating, and copper-resistant bacterium, is distinguished from the urinary pathogens proteus penneri and proteus mirabilis. to further investigate the genetic functions of this strain, the genome sequence and annotation of its open reading frames, which consist of 3,875,927 bp (g+c content, 38.12%), are presented here.201424435854
identification of a proteus penneri isolate as the causal agent of red body disease of the cultured white shrimp penaeus vannamei and its control with bdellovibrio bacteriovorus.bacteriosis has become a major economic problem in the farming of the pacific white shrimp penaeus vannamei. however, no definitive data are available about proteus penneri infection in cultured p. vannamei and its control. in this study, a virulent strain nc was isolated from diseased p. vannamei suffering from red body disease and identified as a p. penneri isolate through phylogenetic analysis and atb 32gn system. a phylogenetic constructed tree using the neighbour-joining method identified t ...201424271474
significance and roles of proteus spp. bacteria in natural environments.proteus spp. bacteria were first described in 1885 by gustav hauser, who had revealed their feature of intensive swarming growth. currently, the genus is divided into proteus mirabilis, proteus vulgaris, proteus penneri, proteus hauseri, and three unnamed genomospecies 4, 5, and 6 and consists of 80 o-antigenic serogroups. the bacteria are known to be human opportunistic pathogens, isolated from urine, wounds, and other clinical sources. it is postulated that intestines are a reservoir of these ...201626748500
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