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transcriptional analysis of the gdha gene in streptococcus thermophilus.to study the transcriptional analysis of glutamate dehydrogenase gene, involved in the amino acid conversion to aroma compound in streptococcus thermophilus.200919486386
[evaluation of prolex for the rapid identification of streptococci isolated in medical microbiology].the need to rapidly identify streptococci responsible for acute infectious diseases has led to the development of agglutination techniques that are able to identify streptococcal group antigens (a, b, c, d, f, and g) directly from primoculture colonies on blood agar. the prolex agglutination tests (pro-lab diagnostics, richmond hill, ontario, canada), distributed in france by i2a, have been used for the determination of group antigens of 166 isolates of streptococci and enterococci previously id ...200415465260
streptococcus infantarius sp. nov., streptococcus infantarius subsp. infantarius subsp. nov. and streptococcus infantarius subsp. coli subsp. nov., isolated from humans and food.eighteen strains isolated from human specimens or from food products were characterized as atypical variants of mannitol-negative streptococcus bovis. they were tested for extended biochemical criteria, ribotyping and dna-dna hybridization in order to define their taxonomic status. these strains were demonstrated to constitute a dna relatedness group that includes strains of dna group 4 of farrow et al. (1984). comparative analysis of 16s rrna sequences demonstrated that these strains represent ...200010939646
african fermented dairy products - overview of predominant technologically important microorganisms focusing on african streptococcus infantarius variants and potential future applications for enhanced food safety and security.milk is a major source of nutrients, but can also be a vehicle for zoonotic foodborne diseases, especially when raw milk is consumed. in africa, poor processing and storage conditions contribute to contamination, outgrowth and transmission of pathogens, which lead to spoilage, reduced food safety and security. fermentation helps mitigate the impact of poor handling and storage conditions by enhancing shelf life and food safety. traditionally-fermented sour milk products are culturally accepted a ...201728364623
changes in equine hindgut bacterial populations during oligofructose-induced laminitis.in the horse, carbohydrate overload is thought to play an integral role in the onset of laminitis by drastically altering the profile of bacterial populations in the hindgut. the objectives of this study were to develop and validate microbial ecology methods to monitor changes in bacterial populations throughout the course of experimentally induced laminitis and to identify the predominant oligofructose-utilizing organisms. laminitis was induced in five horses by administration of oligofructose. ...200616623745
The spiFEG Locus in Streptococcus infantarius subsp. infantarius BAA-102 Confers Protection against Nisin U.Nisin U is a member of the extended nisin family of lantibiotics. Here we identify the presence of nisin U immunity gene homologues in Streptococcus infantarius subsp. infantarius BAA-102. Heterologous expression of these genes in Lactococcus lactis subsp. cremoris HP confers protection to nisin U and other members of the nisin family, thereby establishing that the recently identified phenomenon of resistance through immune mimicry also occurs with respect to nisin.201222064537
[diseases associated with bloodstream infections caused by the new species included in the old streptococcus bovis group].we sought to identify possible diseases associated with bloodstream infections caused by new species of s. bovis group isolated in blood cultures and by studying patient records201222377494
a case of streptococcus gallolyticus subsp. gallolyticus infective endocarditis with colon cancer: identification by 16s ribosomal dna sequencing.although the association between streptococcus bovis endocarditis and colon carcinoma is well known, very few cases of s. bovis infection associated with underlying malignancies have been reported in korea. the s. bovis group has been recently reclassified and renamed as streptococcus gallolyticus and streptococcus infantarius subspecies under a new nomenclature system. we report a case of infective endocarditis with colon cancer caused by s. gallolyticus subsp. gallolyticus (previously named s. ...201020445334
comprehensive study of strains previously designated streptococcus bovis consecutively isolated from human blood cultures and emended description of streptococcus gallolyticus and streptococcus infantarius subsp. coli.modern taxonomy has delineated streptococcus gallolyticus subsp. gallolyticus, s. gallolyticus subsp. pasteurianus, streptococcus infantarius subsp. coli, and s. infantarius subsp. infantarius within the heterogenous group of previously designated clinical streptococcus bovis bacteria. in the present study, 58 consecutive blood culture isolates initially designated s. bovis were further characterized by applying phenotypic and molecular genetic methods, and possible disease associations were inv ...200818614655
streptococcus infantarius sp. nov. related to streptococcus bovis and streptococcus equinus. 19979331678
novel bartonella infection in northern and southern sea otters (enhydra lutris kenyoni and enhydra lutris nereis).since 2002, vegetative valvular endocarditis (vve), septicemia and meningoencephalitis have contributed to an unusual mortality event (ume) of northern sea otters in southcentral alaska. streptococcal organisms were commonly isolated from vegetative lesions and organs from these sea otters. bartonella infection has also been associated with bacteremia and vve in terrestrial mammals, but little is known regarding its pathogenic significance in marine mammals. our study evaluated whether streptoco ...201424629902
bacteraemia due to streptococcus gallolyticus subspecies pasteurianus is associated with digestive tract malignancies and resistance to macrolides and clindamycin.this study was intended to delineate the association between digestive tract malignancies and bacteraemia due to streptococcus gallolyticus subspecies pasteurianus.201424637236
novel real-time pcr assays using taqman minor groove binder probes for identification of fecal carriage of streptococcus bovis/streptococcus equinus complex from rectal swab specimens.real-time pcr based on the recn and gyrb genes was developed to detect four streptococcus bovis/streptococcus equinus complex (sbec) subspecies from rectal swab specimens. the overall prevalence was 35.2%: streptococcus gallolyticus subsp. gallolyticus (11.1%), s. gallolyticus subsp. pasteurianus (13%), streptococcus infantarius subsp. coli (20.4%), and s. infantarius subsp. infantarius (11.1%). to conclude, these real-time pcr assays provide a reliable molecular method to detect sbec pathogenic ...201424391203
taxonomic dissection of the streptococcus bovis group by analysis of manganese-dependent superoxide dismutase gene (soda) sequences: reclassification of 'streptococcus infantarius subsp. coli' as streptococcus lutetiensis sp. nov. and of streptococcus bovis biotype 11.2 as streptococcus pasteurianus sp. nov.the taxonomic dissection of the streptococcus bovis-streptococcus equinus group was carried out upon obtaining sequences for the manganese-dependent superoxide dismutase gene (soda) of the type strains of s. bovis, streptococcus caprinus, s. equinus, streptococcus gallolyticus, streptococcus infantarius, streptococcus macedonicus and streptococcus waius. the soda sequences of 29 streptococcal strains of animal and human origin that were related to s. bovis were also sequenced. a phylogenetic ana ...200212148636
comparative genomics of the dairy isolate streptococcus macedonicus aca-dc 198 against related members of the streptococcus bovis/streptococcus equinus complex.within the genus streptococcus, only streptococcus thermophilus is used as a starter culture in food fermentations. streptococcus macedonicus though, which belongs to the streptococcus bovis/streptococcus equinus complex (sbsec), is also frequently isolated from fermented foods mainly of dairy origin. members of the sbsec have been implicated in human endocarditis and colon cancer. here we compare the genome sequence of the dairy isolate s. macedonicus aca-dc 198 to the other sbsec genomes in or ...201424713045
comparative genome analysis of streptococcus infantarius subsp. infantarius cj18, an african fermented camel milk isolate with adaptations to dairy environment.streptococcus infantarius subsp. infantarius (sii) belongs to the streptococcus bovis/streptococcus equinus complex associated with several human and animal infections. sii is a predominant bacterium in spontaneously fermented milk products in africa. the genome sequence of sii strain cj18 was compared with that of other streptococcus species to identify dairy adaptations including genome decay such as in streptococcus thermophilus, traits for its competitiveness in spontaneous milk fermentation ...201323521820
[streptococcus bovis, taxonomic status, clinical relevance and antimicrobial susceptibility].streptococcus bovis is a large bacterial complex of facultative anaerobic gram-positive cocci that includes distinct, genetically-related species. traditionally, s. bovis was classified into three biotypes: i (mannitol fermentation-positive), ii/1 (mannitol-negative and β-glucuronidase-negative), and ii/2 (mannitol-negative and β-glucuronidase-positive). the introduction of molecular techniques in the last few decades has led to proposals for a genetic classification of this complex: s. bovis bi ...201323453226
bartonella spp. exposure in northern and southern sea otters in alaska and california.since 2002, an increased number of northern sea otters (enhydra lutris kenyoni) from southcentral alaska have been reported to be dying due to endocarditis and/or septicemia with infection by streptococcus infantarius subsp. coli. bartonella spp. dna was also detected in northern sea otters as part of mortality investigations during this unusual mortality event (ume) in kachemak bay, alaska. to evaluate the extent of exposure to bartonella spp. in sea otters, sera collected from necropsied and l ...201425514118
A novel multiplex PCR/RFLP assay for the identification of Streptococcus bovis/Streptococcus equinus complex members from dairy microbial communities based on the 16S rRNA gene.The Streptococcus bovis/Streptococcus equinus complex (SBSEC) comprises pathogenic species associated with different degrees with human infections but also spontaneously fermented dairy products. We aimed therefore at developing a specific identification assay for the SBSEC targeting the 16S rRNA gene comprising a multiplex PCR followed by a differentiating restriction fragment length polymorphisms (RFLP). The multiplex PCR assay was positively applied on 200 SBSEC isolates including reference s ...201222092382
molecular characterization of a novel mosaic tet(s/m) gene encoding tetracycline resistance in foodborne strains of streptococcus bovis.the presence of antibiotic-resistance (ar) genes in foodborne bacteria of enteric origin represents a relevant threat to human health in the case of opportunistic pathogens, which can reach the human gut through the food chain. streptococcus bovis is a human opportunistic pathogen often associated with infections in immune-compromised or cancer patients, and it can also be detected in the environment, including fermented foods. we have focused on the molecular characterization of a tetracycline ...201222723288
adherence characteristics of endocarditis-derived streptococcus gallolyticus ssp. gallolyticus (streptococcus bovis biotype i) isolates to host extracellular matrix proteins.members of the streptococcus bovis group are frequent colonizers of the intestinal tract, which can also cause endocarditis. however, their ability to adhere to and colonize host tissues and the factors associated with pathogenicity are largely unknown. here, we assessed 17 endocarditis-derived human isolates [identified here as 15 streptococcus gallolyticus ssp. gallolyticus (s. bovis biotype i), one s. gallolyticus ssp. pasteurianus (biotype ii/2) and one streptococcus infantarius ssp. coli (b ...200819054100
a traditional sudanese fermented camel's milk product, gariss, as a habitat of streptococcus infantarius subsp. infantarius.samples of the traditional sudanese fermented camel's milk product gariss representing 9 different regions in sudan were microbiologically characterized using an integrated approach including phenotypic and genotypic methods. lactic acid bacteria [log(cfu/g)=7.76-8.66] and yeasts [log(cfu/g)=6.05-7.79] were detected in high numbers. a total of 180 lab isolates were identified of which 123 isolates were found to belong to the streptococcus bovis group. thirteen representative isolates were charac ...200818774196
association between streptococcus infantarius (formerly s. bovis ii/1) bacteremia and noncolonic cancer. 200818256231
novel streptococcus infantarius subsp. infantarius variants harboring lactose metabolism genes homologous to streptococcus thermophilus.streptococcus infantarius subsp. infantarius belongs to the streptococcus bovis/streptococcus equinus complex (sbsec) commonly associated with human and animal infections. we elucidated the lactose metabolism of s. infantarius subsp. infantarius predominant in african fermented milk products. s. infantarius subsp. infantarius isolates (n = 192) were identified in 88% of spontaneously fermented camel milk suusac samples (n = 24) from kenya and somalia at log₁₀ 8.2-8.5 cfu ml⁻¹. african s. infanta ...201222475940
prevalence and comparison of streptococcus infantarius subsp. infantarius and streptococcus gallolyticus subsp. macedonicus in raw and fermented dairy products from east and west africa.streptococcus infantarius subsp. infantarius (sii) and streptococcus gallolyticus subsp. macedonicus are members of the streptococcus bovis/streptococcus equinus complex (sbsec) associated with human infections. sbsec-related endocarditis was furthermore associated with rural residency in southern europe. sbsec members are increasingly isolated as predominant species from fermented dairy products in europe, asia and africa. african variants of sii displayed dairy adaptations to lactose metabolis ...201324131584
reappraisal of the taxonomy of the streptococcus bovis/streptococcus equinus complex and related species: description of streptococcus gallolyticus subsp. gallolyticus subsp. nov., s. gallolyticus subsp. macedonicus subsp. nov. and s. gallolyticus subsp. pasteurianus subsp. nov.'streptococcus bovis/streptococcus equinus' is a large bacterial complex including different species frequently isolated from infections of humans (streptococcus gallolyticus, streptococcus infantarius) or animals (s. bovis, s. equinus, streptococcus alactolyticus). the separation of s. bovis into three different biotypes has been partially correlated with genetic differentiation. in addition, recent advances in bacterial phylogeny have led to the inclusion of streptococcus macedonicus and strep ...200312807180
complete genome sequence of the african dairy isolate streptococcus infantarius subsp. infantarius strain cj18.streptococcus infantarius subsp. infantarius, a member of the streptococcus bovis/streptococcus equinus complex, is highly prevalent in artisanal dairy fermentations in africa. here the complete genome sequence of the dairy-adapted s. infantarius subsp. infantarius cj18 strain--a strain predominant in traditionally fermented camel milk (suusac) from kenya--is presented.201222461547
streptococcus bovis group and biliary tract infections: an analysis of 51 cases.streptococcus bovis is a well-known cause of endocarditis, but its role in other infections has not been well described. we analysed prospectively all patients with biliary tract infections caused by s. bovis group during the period 1988-2011. we selected those cases associated with cholangitis and cholecystitis, defined according to tokyo guidelines. identification of the strains was performed using the api 20 strep and the gp card of the vitek 2 system, and was confirmed by molecular methods. ...201424033711
genomics, evolution, and molecular epidemiology of the streptococcus bovis/streptococcus equinus complex (sbsec).the streptococcus bovis/streptococcus equinus complex (sbsec) is a group of human and animal derived streptococci that are commensals (rumen and gastrointestinal tract), opportunistic pathogens or food fermentation associates. the classification of sbsec has undergone massive changes and currently comprises 7 (sub)species grouped into four branches based on sequences identities: the streptococcus gallolyticus, the streptococcus equinus, the streptococcus infantarius and the streptococcus alactol ...201525233845
streptococcus bovis/s. equinus complex septicemia in a group of calves following intramuscular vaccination.organisms previously classified as streptococcus bovis (i.e., the s. bovis/s. equinus complex) are common in cattle feces, but may also act as opportunistic pathogens. in the current work, streptococcus infantarius subsp. coli, a member of this complex, was associated of a cluster of calves that died within hours of injection with a modified live viral vaccine. within 12 h of vaccination of 46 calves at a cow/calf operation, 4 calves had died, 3 calves were ill, and 1 unvaccinated cow was dead. ...201627216720
genotypic characterization of streptococcus infantarius subsp. coli isolates from sea otters with infective endocarditis and/or septicemia and from environmental mussel samples.pulsed-field gel electrophoresis (pfge) was used to type 128 streptococcus infantarius subsp. coli isolates from sea otters and mussels. six smai pfge groups were detected, with one predominant group representing 57% of the isolates collected over a wide geographic region. several sea otter and mussel isolates were highly related, suggesting that an environmental infection source is possible.201223052307
phylogenetic, epidemiological and functional analyses of the streptococcus bovis/streptococcus equinus complex through an overarching mlst scheme.the streptococcus bovis/streptococcus equinus complex (sbsec) comprises seven (sub)species classified as human and animal commensals, emerging opportunistic pathogens and food fermentative organisms. changing taxonomy, shared habitats, natural competence and evidence for horizontal gene transfer pose difficulties for determining their phylogeny, epidemiology and virulence mechanisms. thus, novel phylogenetic and functional classifications are required. an sbsec overarching multi locus sequence t ...201627329036
carcinogenic properties of proteins with pro-inflammatory activity from streptococcus infantarius (formerly s.bovis).several studies reported linkage between bacterial infections and carcinogenesis. streptococcus bovis was traditionally considered as a lower grade pathogen frequently involved in bacteremia and endocarditis. this bacterium became important in human health as it was shown that 25-80% of patients who presented a s.bovis bacteremia had also a colorectal tumor. moreover, in previous experiments, we demonstrated that s.bovis or s.bovis wall extracted antigens (wea) were able to promote carcinogenesi ...200414742316
re-identification of streptococcus bovis isolates causing bacteraemia according with the new taxonomy criteria: still an issue?all streptococcus bovis blood culture isolates recovered from jan-2003 to jan-2010 (n=52) in our institution were re-identified using new taxonomic criteria based upon their genetic traits. initial identification was performed by the semiautomatic wider system (fco. soria-melguizo, spain) and the api 20 strep (biomérieux, france). all isolates were re-identified by pcr amplification and sequencing of both 16s rdna and soda genes and by mass spectrometry using maldi-tof ms (bruker, germany). resu ...201121752968
changes of the microbial population structure in an overloaded fed-batch biogas reactor digesting maize silage.two parallel, stable operating biogas reactors were fed with increasing amounts of maize silage to monitor microbial community changes caused by overloading. changes of microorganisms diversity revealed by sscp (single strand conformation polymorphism) indicating an acidification before and during the ph-value decrease. the earliest indicator was the appearance of a methanosarcina thermophila-related species. diversity of dominant fermenting bacteria within bacteroidetes, firmicutes and other ba ...201425463789
biofilm forming ability of a new bacterial isolate from dental caries: an atomic force microscopic study.dental plaque being one of the most-studied biofilm communities, is particularly complex because it consists of thousands of bacterial species, and new species are still being isolated and characterized. the aim of the present study is to characterize surface topography of the biofilm formed by a new bacterial isolate, obtained from the dental caries lesion using atomic force microscopy.201425097398
endocarditis caused by streptococcus infantarius subsp. infantarius: a report of two cases. 201424750527
advanced intestinal cancer associated with streptococcus infantarius (former s. bovis ii/1) sepsis. 201324246215
streptococcus infantarius and carcinogenesis: a new chapter in colorectal pathology.as mirrored by several topics throughout history, the causal link between infectious diseases and cancer was initially viewed with disbelief and subsequently forgotten, only to be rediscovered decades later, when it started flourishing into a vibrant multidisciplinary field . just a few years ago, it was estimated that over 20% of all cancers are causally linked to infectious diseases, most frequently caused by bacteria, viruses, and parasites .201324246203
pathogenesis of streptococcus infantarius subspecies coli isolated from sea otters with infective endocarditis.the gram positive bacterial coccus streptococcus infantarius subspecies coli is increasingly linked with development of fatal vegetative infective endocarditis and septicemia in humans, sea otters (enhydra lutris) and other animals. however, the pathogenesis of these infections is poorly understood. using s. infantarius subsp. coli strains isolated from sea otters with infective endocarditis, this study evaluated adherence and invasion of epithelial and endothelial cells, adherence to extracellu ...201525838157
investigating the association between african spontaneously fermented dairy products, faecal carriage of streptococcus infantarius subsp. infantarius and colorectal adenocarcinoma in kenya.consumption of traditional fermented dairy products (tfdp) in africa leads to the ingestion of up to 108streptococcus infantarius subspecies infantarius (sii) per millilitre of spontaneously fermented milk. sii is a member of the streptococcus bovis/streptococcus equinus complex (sbsec) for which some members are associated particularly with colorectal cancer or endocarditis. the extent of health risks to tfdp consumers is largely unknown. a hospital-based unmatched case-control study was conduc ...201829079186
enrichment of isoflavone aglycones in soymilk by fermentation with single and mixed cultures of streptococcus infantarius 12 and weissella sp. 4.soymilk was fermented with either streptococcus infantarius 12 (si 12), weissella sp. 4 (ws 4), or their mixed cultures with different mixing ratios (si 12:ws 4=1:1, 1:3, 1:5, and 1:10, v/v) for 12h at 37°c. all cultures in soymilk readily proliferated and reached about 10(8-9)cfu/ml. after 12h, ph and titratable acidity of soymilk ranged 4.19-4.47 and 0.57%-0.64%, respectively. the ph of soymilk fermented with si 12 was the lowest while that obtained with ws 4 the highest. a sharp increase in β ...200826003348
a fish mouth appearing perforation of the native aortic valve due to streptococcus infantarius subspecies coli.infective endocarditis due to streptococcus infantarius with the subspecies (subsp.) coli is infrequently encountered in healthy humans. this entity is associated with hepatobiliary malignancies and colorectal neoplasia. here, we report on a unique case of endocarditis associated with s. infantarius subsp. coli in an 80-year-old male with no known risk factors of the infective endocarditis.201728863252
bacterial diversity of the colombian fermented milk "suero costeño" assessed by culturing and high-throughput sequencing and dgge analysis of 16s rrna gene amplicons."suero costeño" (sc) is a traditional soured cream elaborated from raw milk in the northern-caribbean coast of colombia. the natural microbiota that characterizes this popular colombian fermented milk is unknown, although several culturing studies have previously been attempted. in this work, the microbiota associated with sc from three manufacturers in two regions, "planeta rica" (córdoba) and "caucasia" (antioquia), was analysed by means of culturing methods in combination with high-throughput ...201728800820
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