| burkholderia uboniae sp. nov., l-arabinose-assimilating but different from burkholderia thailandensis and burkholderia vietnamiensis. | a polar multitrichous gram-negative motile rod, ey 3383, originally identified as burkholderia thailandensis, revealed a dna-dna reassociation rate of 36.7%, under stringent conditions, with the type strain of b. thailandensis, despite the 16s rdna homology value between two type strains being as high as 97.9%. the strain was clearly differentiated from the type strain of b. thailandensis by physiological, bio-chemical, and nutritional characteristics, without significant difference in cellular ... | 2000 | 10832977 |
| expanded range of burkholderia species in australia. | this study describes the isolation and characterization of several burkholderia species from soil in northern australia. phenotypic and molecular tests indicate that these isolates belong to the species burkholderia thailandensis and burkholderia ubonensis. these observations significantly extend our knowledge of the geographic distribution of these 2 species. evidence of these species in australia has implications for bacterial identification in clinical laboratories, diagnostic serology tests, ... | 2008 | 18385355 |
| burkholderia latens sp. nov., burkholderia diffusa sp. nov., burkholderia arboris sp. nov., burkholderia seminalis sp. nov. and burkholderia metallica sp. nov., novel species within the burkholderia cepacia complex. | the taxonomic position of five reca gene clusters of burkholderia cepacia complex (bcc) isolates was determined using a polyphasic taxonomic approach. the levels of 16s rrna and reca gene sequence similarity, multilocus sequence typing (mlst) data and the intermediate dna-dna binding values demonstrated that these five clusters represented five novel species within the bcc. biochemical identification of these species is difficult, as is the case for most bcc species. however, identification of t ... | 2008 | 18599699 |
| molecular characterization of burkholderia strains isolated from rice cultivars (oryza sativa l.) for species identification and phylogenetic grouping. | the genus burkholderia consists of extremely versatile bacteria that occupy diverse niches and are commonly encountered in the rhizosphere of crop plants. in this study, we characterized three plant growth promoting strains assigned as burkholderia sp. using biochemical and molecular characterization. the burkholderia spp. strains cbmb40, cbpb-him, and cbpb-hod were characterized using biochemical tests, biolog carbon substrate utilization, fatty acid methyl ester analysis, analysis of reca gene ... | 2008 | 18600039 |
| microbial diversity of traditional vietnamese alcohol fermentation starters (banh men) as determined by pcr-mediated dgge. | the diversity of fungi and bacteria associated with traditional vietnamese alcohol fermentation starters (banh men) was investigated by pcr-mediated dgge. from 52 starter samples, 13 species of fungi (including yeasts) and 23 species of bacteria were identified. the fungal composition of the starters was consistent with little variation among samples. it consisted of amylase producers (rhizopus oryzae, r. microsporus, absidia corymbifera, amylomyces sp., saccharomycopsis fibuligera), ethanol pro ... | 2008 | 18838186 |
| cloning and biochemical characterization of a novel lipolytic gene from activated sludge metagenome, and its gene product. | in this study, a putative esterase, designated estmy, was isolated from an activated sludge metagenomic library. the lipolytic gene was subcloned and expressed in escherichia coli bl21 using the pet expression system. the gene estmy contained a 1,083 bp open reading frame (orf) encoding a polypeptide of 360 amino acids with a molecular mass of 38 kda. sequence analysis indicated that it showed 71% and 52% amino acid identity to esterase/lipase from marine metagenome (acl67845) and burkholderia u ... | 2010 | 21054894 |
| antibiosis of burkholderia ubonensis againist burkholderia pseudomallei, the causative agent for melioidosis. | melioidosis, caused by burkholderia pseudomallei, is an enigmatic infectious disease that afflicts individuals in many tropical and developing regions. treatment is hampered by the organism's innate antibiotic resistance and the disease's non-pathognomic presentation. recently, added attention has been given to this organism due to its classification as a potential biowarfare agent. therefore, methods of preventing acquisition of infection are needed. we investigated antagonism between burkholde ... | 2010 | 21073065 |
| accurate and rapid identification of the burkholderia pseudomallei near-neighbour, burkholderia ubonensis, using real-time pcr. | burkholderia ubonensis is an environmental bacterium belonging to the burkholderia cepacia complex (bcc), a group of genetically related organisms that are associated with opportunistic but generally nonfatal infections in healthy individuals. in contrast, the near-neighbour species burkholderia pseudomallei causes melioidosis, a disease that can be fatal in up to 95% of cases if left untreated. b. ubonensis is frequently misidentified as b. pseudomallei from soil samples using selective culturi ... | 2013 | 23967229 |
| perceiving the chemical language of gram-negative bacteria: listening by high-resolution mass spectrometry. | gram-negative bacteria use n-acylhomoserine lactones (ahls) as their command language to coordinate population behavior during invasion and colonization of higher organisms. although many different bacterial bioreporters are available for ahls monitoring, in which a phenotypic response, e.g. bioluminescence, violacin production, and β-galactosidase activity, is exploited, mass spectrometry (ms) is the most versatile detector for rapid analysis of ahls in complex microbial samples, with or withou ... | 2013 | 22986985 |