| [plerocercoid infestation of the thailand reptiles in the berlin zoo]. | plerocercoids were found under the skin and in the body cavity of ahaetulla nastua, boiga multimaculata, trimeresurus purpureomaculatus, and varanus dumerilii, imported from thailand. starting with these plerocercoids the cestode cycle was experimentally traced in the laboratory (lueheela = spirometra sp.). domestic cat and domestic dog served as definitive hosts, native copepods at first intermediate hosts, native anurans, reptiles, and chicken as second intermediate hosts and paratenic hosts. ... | 1976 | 1267224 |
| isolation and characterization of the major phospholipase a2 from the venom of trimeresurus purpureomaculatus (shore pit viper). | 1. the major phospholipase a2 (pla-de4) of the venom of trimeresurus purpureomaculatus (shore pit viper) has been purified to electrophoretic homogeneity. 2. the isoelectric point of the purified enzyme was determined to be 4.20, and the mol. wt was 31,700 as estimated by sephadex g-75 gel filtration chromatography; and 14,000 as estimated by sds-polyacrylamide gel electrophoresis. the purified enzyme hydrolyzed phosphatidylcholine (pc) faster than phosphatidylethanolamine (pe), whereas phosphat ... | 1989 | 2612728 |
| purpureotin: a novel di-dimeric c-type lectin-like protein from trimeresurus purpureomaculatus venom is stabilized by noncovalent interactions. | purpureotin, a novel di-dimeric c-type lectin-like protein (clp) from trimeresurus purpureomaculatus, was purified and sequenced. while its native molecular mass was determined to be 63kda, purpureotin showed a single band of 30kda on nonreducing sds-page and two polypeptide chains (16.0 and 14.5kda) under reducing condition. these results were subsequently confirmed by mass spectrometric analyses. based on these results, we postulate that purpureotin is a dimer of the alpha,beta-heterodimer whi ... | 2004 | 15019836 |
| isolation and characterization of the thrombin-like enzyme from cryptelytrops purpureomaculatus venom. | a thrombin-like enzyme, purpurase, was purified from the cryptelytrops purpureomaculatus (mangrove pit viper) venom using high performance ion-exchange and gel filtration chromatography. the purified sample (termed purpurase) yielded a homogeneous band in sds-polyacrylamide gel electrophoresis with a molecular weight of 35,000. the n-terminal sequence of purpurase was determined to be vvggdecnindhrslvrif and is homologous to many other venom thrombin-like enzymes. purpurase exhibits both arginin ... | 2010 | 19770070 |
| partial purification of acetylcholinesterase from the venom of the shore pit viper (trimeresurus purpureomaculatus). | trimeresurus purpureomaculatus venom acetylcholinesterase has been partially purified by sephadex g-200 gel filtration chromatography and deae sephacel ion exchange chromatography. the enzyme has a mol. wt of 58,600. it was strongly inhibited by physostigmine salicylate and edrophonium chloride and exhibited substrate inhibition at high substrate concentration. the content of acetylcholinesterase in trimeresurus purpureomaculatus venom was estimated to be much less than 0.3%. | 2007 | 3188057 |
| snakebite by the shore pit viper (trimeresurus purpureomaculatus) treated with polyvalent antivenom. | although snakebites are uncommon, there are several species of medically important venomous snakes native to singapore. we present a case of envenoming by the shore pit viper (trimeresurus purpureomaculatus) that showed clinical improvement when treated with the indian (haffkine) polyvalent antivenom. a 40-year-old man was bitten on his right hand by a snake, which was identified through photos and his description to be a shore pit viper, which is native to the local mangrove area. severe swelli ... | 2016 | 27061038 |
| cross reactivity and lethality neutralization of venoms of indonesian trimeresurus complex species by thai green pit viper antivenom. | arboreal pit vipers of the trimeresurus complex group are medically important species in indonesia (west of wallace's line), but there is no specific antivenom produced in the country for treating related envenomation. instead, the exiting trivalent indonesian antivenom, biosave®serum anti bisa ular (sabu, indicated for envenoming by malayan pit viper, javan spitting cobra and banded krait) is often misused to treat trimeresus envenoming resulting in poor therapeutic outcome. here, we investigat ... | 2017 | 29051104 |
| a hemorrhagin as a metalloprotease in the venom of trimeresurus purpureomaculatus: purification and characterization. | a major hemorrhagin was purified from the venom of the thai green pit viper (trimeresurus purpureomaculatus) by gel filtration, ion-exchange and affinity chromatography. a 15-fold purification was achieved with an overall yield of 7% of hemorrhagic activity. the hemorrhagin was homogeneous according to disc- and sds-page as well as on immunodiffusion. the molecular weight determined by sds-page was 72kda. the purified hemorrhagin expresses proteolytic activity with heat-denatured casein and hide ... | 2002 | 11738239 |
| proteomic characterization and comparison of malaysian tropidolaemus wagleri and cryptelytrops purpureomaculatus venom using shotgun-proteomics. | tropidolaemus wagleriandcryptelytrops purpureomaculatusare venomous pit viper species commonly found in malaysia. tandem mass spectrometry analysis of the crude venoms has detected different proteins int. wagleriandc. purpureomaculatus. they were classified into 13 venom protein families consisting of enzymatic and nonenzymatic proteins. enzymatic families detected int. wagleriandc. purpureomaculatusvenom were snake venom metalloproteinase, phospholipase a₂, ʟ-amino acid oxidase, serine protease ... | 2016 | 27763534 |
| isolation, characterization and antigenic cross-reactivities of the major hemorrhagin from cryptelytrops purpureomaculatus venom. | the major hemorrhagin from c. purpureomaculatus (mangrove pit viper) venom was purified to homogeneity and termed maculatoxin. maculatoxin has a molecular weight of 38 kda as determined by sds-page. it is an acidic protein (pi= 4.2) and exhibited proteolytic and hemorrhagic activities (mhd10 = 0.84 microg in mice) but was not lethal to mice at a dose of 1 microg/g. the hemorrhagic activity of maculatoxin was completely inactivated by edta and partially inhibited by atp and citrate. the n-termina ... | 2013 | 24579371 |
| pharmacokinetics of cryptelytrops purpureomaculatus (mangrove pit viper) venom following intravenous and intramuscular injections in rabbits. | the pharmacokinetic profiles of cryptelytrops purpureomaculatus (mangrove pit viper) venom following intravenous and intramuscular injections were investigated in rabbits. the serum levels of the venom were estimated using double-sandwich enzyme-linked immunosorbent assay (elisa). after intravenous injection (0.2 mg/kg), the serum venom concentration–time course declined in a biexponential manner, consistent with a two-compartment model, with an α-phase half-life of 0.25 h and a β-phase half-lif ... | 2013 | 24455773 |
| biological properties of trimeresurus purpureomaculatus (shore pit viper) venom and its fractions. | the toxic and biological activities of four samples of trimeresurus purpureomaculatus venom were examined. the lethality, protein composition and biological activities of the four venom samples were similar. three of the venom samples had ld50 (i.v.) values of 0.9 micrograms/g while the fourth had a lower ld50 (i.v.) of 0.45 micrograms/g. all four venom samples exhibited hemorrhagic, edema-inducing, anticoagulant and thrombin-like activities as well as the usual enzymes found in crotalid venoms. ... | 1988 | 3245058 |
| investigating the cytotoxic effects of the venom proteome of two species of the viperidae family (cerastes cerastes and cryptelytrops purpureomaculatus) from various habitats. | animal secretions are of great interest in terms of drug development due to their complex protein and peptide composition. especially, in the field of therapeutic medications such as anti-cancer drugs snake venoms receive attention. in this study, we address two viperidae species from various habitats with a particular focus on the cytotoxic potential along with the decomplexation of the venom proteome: the horned desert viper (cerastes cerastes), native to desert regions of north africa and the ... | 2019 | 30825636 |
| proteomics and preclinical antivenom neutralization of the mangrove pit viper (trimeresurus purpureomaculatus, malaysia) and white-lipped pit viper (trimeresurus albolabris, thailand) venoms. | | 2020 | 32442435 |
| cytotoxic and anticancer properties of the malaysian mangrove pit viper (trimeresurus purpureomaculatus) venom and its disintegrin (purpureomaculin). | the asiatic pit vipers from the trimeresurus complex are medically important venomous snakes. these pit vipers are often associated with snakebite that leads to fatal coagulopathy and tissue necrosis. the cytotoxic venoms of trimeresurus spp.; however, hold great potential for the development of peptide-based anticancer drugs. | 2020 | 32742279 |
| cytotoxic, anti-proliferative and apoptosis activity of l-amino acid oxidase from malaysian cryptelytrops purpureomaculatus (cp-laao) venom on human colon cancer cells. | the aim of this study is to investigate the potential anti-cancer activity of l-amino acid oxidase (cp-laao) purified from the venom of cryptelytrops purpureomaculatus on sw480 and sw620 human colon cancer cells. mass spectrometry guided purification was able to identify and purify cp-laao. amino acid variations identified from the partial protein sequence of cp-laao may suggest novel variants of these proteins. the activity of the purified cp-laao was confirmed with o-phenyldiamine (opd)-based ... | 2018 | 29890640 |