| analysis of the hexon gene sequence of bovine adenovirus type 4 provides further support for a new adenovirus genus (atadenovirus). | the putative hexon gene of bovine adenovirus type 4 (bav-4), encoding 910 amino acid residues, has been identified and sequenced. a characteristic codon usage biased towards the use of at-rich triplets was observed. comparative analysis with other hexon sequences detected a high level of amino acid identity in the regions corresponding to the pedestals of the hexon. substitutions, insertions and deletions were identified mainly in the variable regions forming the loops which are exposed on the o ... | 1998 | 9634088 |
| dna sequence of frog adenovirus. | the genome of frog adenovirus (fradv-1) was sequenced and found to be the smallest of all known adenovirus genomes. the sequence obtained was 26163 bp in size and contains a substantial direct repeat near the right terminus, implying that it was derived by recombination from a parental genome of only 25517 bp. the closest relative of fradv-1 proved to be turkey adenovirus 3, an avian adenovirus with no previously known near relative. sequence comparisons showed that the two viruses have equivale ... | 2000 | 10993931 |
| four new inverted terminal repeat sequences from bovine adenoviruses reveal striking differences in the length and content of the itrs. | the inverted terminal repeat (itr) of the genome of four bovine adenovirus (badv) types have been sequenced, analysed and compared to the itrs of other adenoviruses. the length of itrs of the examined badvs ranged between 59 and 368 base pairs, thus the presently known longest adenovirus itr sequence is from badv-10. the conserved motifs and characteristic sequence elements of the itrs providing different binding sites for replicative proteins of viral and cellular origin seemed to be distribute ... | 2001 | 11324754 |
| reptile adenoviruses in cattle? | this paper describes a hypothesis on the origin of the members of the recently established adenovirus genus, atadenovirus, invading cattle, sheep, deer, duck and poultry. comparison of the phylogenetic trees of adenoviruses and their hosts suggests a very ancient but common origin for the atadenoviruses. the surprisingly large difference between these virus types and other adenoviruses infecting the same host can be easily understood by assuming their separate evolution in different hosts (e.g., ... | 2000 | 11402665 |
| characterization of a new adenovirus isolated from black-tailed deer in california. | an adenovirus associated with systemic and localized vascular damage was demonstrated by transmission electron microscopy and immunohistochemistry in a newly recognized epizootic hemorrhagic disease in california black-tailed deer. in this study, we describe the cultural, physicochemical and serological characteristics of a virus isolated from lung using neonatal white-tail deer lung and turbinate cell cultures. the virus had the cultural, morphological and physicochemical characteristics of mem ... | 2001 | 11504424 |
| molecular confirmation of an adenovirus in brushtail possums (trichosurus vulpecula). | partial genome characterisation of a non-cultivable marsupial adenovirus is described. adenovirus-like particles were found by electron microscopy (em) in the intestinal contents of brushtail possums (trichosurus vulpecula) in new zealand. using degenerate pcr primers complementary to the most conserved genome regions of adenoviruses, the complete nucleotide sequence of the penton base gene, and partial nucleotide sequences of the dna polymerase, hexon, and pvii genes were obtained. phylogenetic ... | 2002 | 11864751 |
| sequence analysis of porcine adenovirus serotype 5 fibre gene: evidence for recombination. | the nucleic acid and deduced amino acid sequence of the fibre gene of the hnf-61 strain of porcine adenovirus serotype 5 (padv-5) was determined and compared to that of the hnf-70 strain of the same serotype (nagy et al., j gen virol 82, 525-529, 2001) and also to adenovirus fibre genes from the genera mastadenovirus and atadenovirus. the putative hnf-61 and hnf-70 proteins were similar to each other, with 90% amino acid identity. conserved amino acid sequences described for mastadenovirus fibre ... | 2002 | 12018710 |
| odocoileus hemionus deer adenovirus is related to the members of atadenovirus genus. | the odocoileus hemionus deer adenovirus (odadv-1) causes systemic and local vasculitis and proves extremely lethal for mule deer. to characterize the virus, part of the genome flanking the fiber gene was cloned and sequenced. the sequence revealed two open-reading frames that mapped to pviii hexon-associated protein precursor and fiber protein of several other adenoviruses. the highest amino acid homology for pviii and fiber was found with the members of the proposed atadenovirus genus: ovine ad ... | 2002 | 12038693 |
| bovine adenovirus serotype 7 infections in postweaning calves. | to detect bovine adenovirus serotype 7 (bav-7) infections in calves by use of viral isolation and serologic testing. | 2002 | 12118678 |
| genetic organization and sequence analysis of pviii, fiber and early region 4 of bovine adenovirus type 7. | the dna sequence of 8,810 nucleotides at the right end of bovine adenovirus type 7 (bav7) genome was determined and compared with similar regions of other adenoviruses. this genomic region of bav7 consists of sequences encoding partial 33k, pviii, fiber, putative early region 4 (e4) proteins and other unassigned proteins. however, bav7 e3 region is not present in the expected location between pviii and fiber as bav7 intergenic region between pviii and fiber genes is only 183 nucleotides. the pre ... | 2002 | 12206309 |
| first molecular evidence for the existence of distinct fish and snake adenoviruses. | from adenovirus-like viruses originating from a fish and a snake species, a conserved part of the adenoviral dna polymerase gene was pcr amplified, cloned and sequenced. phylogenetic analysis showed that the snake adenovirus is closely related to the members of the proposed genus atadenovirus, whereas the fish isolate seems to represent a separate cluster, likely a new genus. | 2002 | 12208985 |
| genomic and phylogenetic analyses of an adenovirus isolated from a corn snake (elaphe guttata) imply a common origin with members of the proposed new genus atadenovirus. | approximately 60% of the genome of an adenovirus isolated from a corn snake (elaphe guttata) was cloned and sequenced. the results of homology searches showed that the genes of the corn snake adenovirus (snadv-1) were closest to their counterparts in members of the recently proposed new genus atadenovirus: in phylogenetic analyses of the complete hexon and protease genes, snadv-1 indeed clustered together with the atadenoviruses. the characteristic features in the genome organization of snadv-1 ... | 2002 | 12237421 |
| biology of ovine adenovirus infection of nonpermissive cells. | nonhuman adenoviruses, including those of the genus atadenovirus, have the potential to serve as vectors for vaccine and gene therapy applications in humans, since they are resistant to preexisting immunity induced by human adenoviruses in the majority of the population. in this study, we elucidate the outcome of infection by ovine adenovirus type 7 isolate 287 (oadv) of several nonovine cell types. we show here that oadv infects a wide range of nonovine cells but is unable to complete its repli ... | 2002 | 12368331 |
| the p32k structural protein of the atadenovirus might have bacterial relatives. | the primary structure of a novel adenoviral protein referred to as p32k and found exclusively in members of the proposed new genus atadenovirus was analyzed. the p32k gene sequence was determined from two bovine and one snake adenovirus types. altogether five different p32k sequences were examined, two of them were obtained from the gene bank. the c-terminal part of the protein is conserved and shares similarity with certain bacterial small acid soluble proteins (sasps). the sequence similarity ... | 2003 | 12574864 |
| construction, rescue, and characterization of vectors derived from ovine atadenovirus. | gene transfer vectors derived from ovine atadenovirus type 7 (oadv) can efficiently infect a variety of mammalian cells in vitro and in vivo to deliver and express transgenes. however, early oadv vectors were designed on human mastadenovirus principles prior to the complete characterization of oadv genes and transcripts. the distinctive arrangement of the oadv genome has suggested ways to improve oadv vector design and utility. we therefore developed a cosmid-based approach that allows efficient ... | 2003 | 14581531 |
| gene-directed enzyme prodrug therapy for prostate cancer in a mouse model that imitates the development of human disease. | gene-directed enzyme prodrug therapy (gdept) based on the e. coli enzyme purine nucleoside phosphorylase (pnp) represents a new approach for treating slow growing tumours like prostate cancer (pca). expressed enzyme converts a systemically administered prodrug, fludarabine phosphate, to a toxic metabolite, 2-fluoroadenine. infected and neighbouring cells are killed by a bystander effect that results from the inhibition of dna and rna synthesis. | 2004 | 14716676 |
| identification of an ovine atadenovirus gene whose product activates the viral e2 promoter: possible involvement of e2f-1. | activation of the adenoviral e2 promoter is an early step in adenovirus gene expression. for members of the mast- and aviadenoviruses, this requires induction of the cellular transcription factor e2f by virally encoded gene products such as e1a, e4orf6/7 and orf22/gam-1. the newly recognized genus atadenovirus, of which the ovine isolate oadv is the prototype, lacks any sequence homology to those genes. to find a possible link between e2 promoter activation and oadv gene expression, we utilized ... | 2004 | 14972537 |
| detection of bovine and porcine adenoviruses for tracing the source of fecal contamination. | in this study, a molecular procedure for the detection of adenoviruses of animal origin was developed to evaluate the level of excretion of these viruses by swine and cattle and to design a test to facilitate the tracing of specific sources of environmental viral contamination. two sets of oligonucleotides were designed, one to detect porcine adenoviruses and the other to detect bovine and ovine adenoviruses. the specificity of the assays was assessed in 31 fecal samples and 12 sewage samples th ... | 2004 | 15006765 |
| abolition of hcar-dependent cell tropism using fiber knobs of atadenovirus serotypes. | most adenoviral vectors use in gene therapy protocols derive from species c. however, expression of the primary receptor (human coxsackievirus and adenovirus receptor, hcar) for these adv is variable on cancer cells. in vivo targeting of a therapeutic gene to specific cells has then become a major issue in gene therapy. the ad fiber protein largely determines viral tropism through interaction with specific receptors. hereto, we constructed a set of hadv5 vectors carrying chimeric fibers with kno ... | 2004 | 15051380 |
| ovine atadenovirus: a review of its biology, biosafety profile and application as a gene delivery vector. | the ovine adenovirus isolate oadv287 is the prototype of the newly recognized genus of atadenoviruses. although not as well studied as human mastadenoviruses, a substantial amount of work has now been carried out with this virus and an understanding of its interesting and unique properties is beginning to emerge. in this article the biology and biosafety profile of the virus is reviewed. this knowledge underpins the exploitation of the virus as a gene delivery vector. its potential as a vaccine ... | 2004 | 15061773 |
| recombinant ovine atadenovirus induces a strong and sustained t cell response against the hepatitis c virus ns3 antigen in mice. | ovine atadenovirus (oadv) is a novel gene transfer vector with excellent in vivo gene transfer characteristics. in the present study, we have investigated the ability of an oadv vector to mediate a t cell response to an antigen of the hepatitis c virus (hcv) in mice. specifically, an expression cassette coding for non-structural protein 3 (ns3) of hepatitis c virus was inserted into the oadv genome and the resulting recombinant virus (oadv-ns3) was shown to propagate stably and to express the ns ... | 2004 | 15246602 |
| preclinical evaluation of a prostate-targeted gene-directed enzyme prodrug therapy delivered by ovine atadenovirus. | gene-directed enzyme prodrug therapy (gdept) based on the escherichia coli enzyme, purine nucleoside phosphorylase (pnp), provides a novel strategy for treating slowly growing tumors like prostate cancer (cap). pnp converts systemically administered prodrug, fludarabine phosphate, to a toxic metabolite, 2-fluoroadenine, that kills pnp-expressing and nearby cells by inhibiting dna, rna and protein synthesis. reporter gene expression directed by a hybrid prostate-directed promoter and enhancer, ps ... | 2004 | 15343359 |
| purine nucleoside phosphorylase and fludarabine phosphate gene-directed enzyme prodrug therapy suppresses primary tumour growth and pseudo-metastases in a mouse model of prostate cancer. | gene-directed enzyme prodrug therapy based on the e. coli purine nucleoside phosphorylase (pnp) gene produces efficient tumour cell killing. pnp converts adenosine analogs into toxic metabolites that diffuse across cell membranes to kill neighbouring untransduced cells (pnp-gdept). interference with dna, rna and protein synthesis kills dividing and non-dividing cells, an important consideration for slow-growing prostate tumours. this study examined the impact of administering pnp-gdept into orth ... | 2004 | 15493036 |
| detection and analysis of six lizard adenoviruses by consensus primer pcr provides further evidence of a reptilian origin for the atadenoviruses. | a consensus nested-pcr method was designed for investigation of the dna polymerase gene of adenoviruses. gene fragments were amplified and sequenced from six novel adenoviruses from seven lizard species, including four species from which adenoviruses had not previously been reported. host species included gila monster, leopard gecko, fat-tail gecko, blue-tongued skink, tokay gecko, bearded dragon, and mountain chameleon. this is the first sequence information from lizard adenoviruses. phylogenet ... | 2004 | 15542689 |
| isolation of bovine adenovirus serotype 6 from a calf in the united kingdom. | two viruses, designated 99-8130(c) and 99-8130(i), were isolated in calf testis cells from the colon and ileum, respectively, of a suckled beef calf which had developed dysentery and died. electron microscopy indicated that the mean (sd) size of the viral particles, 83 (2.5) nm, and their morphology were consistent with their being members of the family adenoviridae. they were confirmed as adenoviruses by pcr when products of the expected size (608 bp) were amplified from both isolates by using ... | 2005 | 15689037 |
| the effect of paralogous lineages on the application of reconciliation analysis by cophylogeny mapping. | paralogy defines similarity caused by duplication rather than common descent and is well known in the case of paralogous gene copies within a single genome. the term is here extended to paralogous lineages of associates within a single host. the phylogenies of four genera within the herpesviridae were reconciled with host phylogenies using cophylogenetic mapping. the observed correspondence for each pair of phylogenies was evaluated through randomization of the viral phylogeny and demonstrated t ... | 2005 | 15805015 |
| lh3, a "homologue" of the mastadenoviral e1b 55-kda protein is a structural protein of atadenoviruses. | ovine adenovirus serotype 7 (oadv), the prototype atadenovirus, has gene homologues for most mastadenovirus structural proteins but lacks proteins v and ix. instead, oadv has structural proteins of 32 and 42 kda although the gene encoding the latter had not previously been identified. the presently reported studies of oadv virions have now identified a minor structural polypeptide of approximately 40 kda as the product of the l1 52/55-kda gene and, more surprisingly, shown that the 42-kda protei ... | 2005 | 16112161 |
| an adenovirus serotype 5 vector with fibers derived from ovine atadenovirus demonstrates car-independent tropism and unique biodistribution in mice. | many clinically important tissues are refractory to adenovirus (ad) infection due to negligible levels of the primary ad5 receptor the coxsackie and adenovirus receptor car. thus, development of novel car-independent ad vectors should lead to therapeutic gain. ovine atadenovirus type 7, the prototype member of genus atadenovirus, efficiently transduces car-deficient human cells in vitro, and systemic administration of oadv is not associated with liver sequestration in mice. the penton base of oa ... | 2006 | 16516257 |
| b subunit of e. coli enterotoxin as adjuvant and carrier in oral and skin vaccination. | mucosal sites are one of the main natural ports of entry into the body. stimulation of a local response by antibodies as the systemic protection may enhance the efficacy of non-living vaccines, and allow for vaccination by subunit vaccines without the need for injection. mucosal or skin vaccination necessitates a suitable adjuvant and carrier. escherichia coli heat-labile enterotoxin (lt) and its b subunit (ltb) have been found to be effective adjuvants. the aim of this study was to efficiently ... | 2006 | 16701905 |
| serological survey of racing pigeons for selected pathogens in taiwan. | to investigate the pathogens that racing pigeons in taiwan are exposed to, a total of 3764 pigeons from 90 lofts were analysed by collection of blood samples in the period between october 2000 and september 2001. the haemagglutination inhibition (hi) test was performed to detect antibodies against newcastle disease virus (ndv), type 2 avian paramyxovirus (apmv-2), and egg drop syndrome '76 virus (eds-76v). the agar-gel precipitin (agp) test was used to detect antibodies against fowl adenovirus ( ... | 2006 | 16841756 |
| production and release testing of ovine atadenovirus vectors. | gene-directed enzyme prodrug therapy (gdept) is an emerging approach for the treatment of cancers. a variety of viral vectors have been used to deliver genes that encode the relevant enzymes, and some have been tested in clinical trials. to ensure the potency and efficacy of such vectors and to obtain regulatory approval to administer them to humans, it is necessary to develop a suite of assays that provide quality assurance. new gdept vectors based on ovine atadenovirus and escherichia coli pur ... | 2007 | 17401165 |
| construction of capsid-modified recombinant bovine adenovirus type 3. | adenoviruses have become a popular vehicle for gene transfer into animal and human cells. however, wide prevalence of preexisting immunity to human adenovirus (hadv) and the promiscuous nature of the virus have made the use of nonhuman adenoviruses an attractive alternative. moreover, readministration of viral vectors is often required to maintain therapeutic levels of transgene expression, resulting in vector-specific immune responses. although a number of features of bovine adenovirus (badv)-3 ... | 2007 | 17401166 |
| detection of antibodies to egg drop syndrome virus in chicken serum using a field-based immunofiltration (flow-through) test. | a simple, user-friendly, and rapid method to detect the presence of antibodies to egg drop syndrome 76 (eds) virus in chicken sera based on an immunofiltration (flow-through) test was developed. purified eds virus antigen was coated onto nitrocellulose membranes housed in a plastic module with layers of absorbent filter pads underneath. following addition of serum to be tested and washing, monoclonal antibodies or polyclonal serum to chicken immunoglobulin g (igg) was used as a bridge antibody t ... | 2007 | 17992943 |
| effect of selection for phagocytosis in dwarf chickens on immune and reproductive characters. | in current study, phagocytosis product (pp) of peripheral blood monocytes was detected among 920 dwarf chickens (460 per sex) at 20 wk of age, and based on discrepancies of pp, the flock was grouped (the highest group, the medium group, and the lowest group). then serum hemagglutination inhibition antibody titers and subpopulations of t-lymphocytes of each group were examined after inoculations of avian influenza virus h5n2 inactivated vaccine (20 wk of age), avian influenza virus h9 inactivated ... | 2008 | 18079448 |
| completion of the genome analysis of snake adenovirus type 1, a representative of the reptilian lineage within the novel genus atadenovirus. | genome sequencing and analysis of snake adenovirus type 1 (snadv-1), originating from corn snake, were completed. this is the first full genomic sequence of an adenovirus from reptilian hosts. the presence of characteristic genus-common genes and transcription units, showed that snadv-1 shares similar genome organisation with members of the recently established genus atadenovirus. three novel open reading frames of yet unknown functions were found. one of these seemed to be related to a putative ... | 2008 | 18166240 |
| evaluation of the pathogenic potential of cervid adenovirus in calves. | four 3-month-old jersey calves and three 3-month-old holstein calves were inoculated with cervid adenovirus and monitored for clinical signs until necropsied between 10 and 42 days postinoculation. the neonatal jersey calves had received colostrum, and the holstein calves were colostrum deprived. preinoculation and postinoculation serum samples were tested for antibodies to the cervid adenovirus, bovine adenovirus type 6, bovine adenovirus type 7, and goat adenovirus type 1. virus isolation was ... | 2008 | 18182505 |
| recombinant egg drop syndrome subunit vaccine offers an alternative to virus propagation in duck eggs. | egg drop syndrome (eds) virus vaccines are routinely produced in embryonated duck eggs (solyom et al., 1982). this procedure poses the risk of dissemination of pathogens, such as avian influenza virus, as the eggs used are not from specific pathogen free birds. to address this problem, the knob and part of the shaft domain of the fibre protein of the eds virus (termed knob-s) were expressed in escherichia coli and assessed as a subunit vaccine. a single vaccination with the recombinant protein i ... | 2008 | 18202947 |
| serologic and hexon phylogenetic analysis of ruminant adenoviruses. | the objectives of this study were to determine the antigenic relationship among ruminant adenoviruses and determine their phylogenetic relationship based on the deduced hexon gene amino acid sequence. results of reciprocal cross-neutralization tests demonstrated antigenic relationships in either one or both directions among bovine adenovirus type 6 (badv-6), badv-7, ovine adenovirus type 7 (oadv-7), caprine adenovirus type 1 (gadv-1), and deer adenovirus (odocoileus adenovirus 1, odadv-1). no an ... | 2008 | 18340400 |
| cryoelectron microscopy map of atadenovirus reveals cross-genus structural differences from human adenovirus. | a three-dimensional (3d) cryoelectron microscopy reconstruction of the prototype atadenovirus (oadv [an ovine adenovirus isolate]) showing information at a 10.6-a resolution (0.5 fourier shell correlation) was derived by single-particle analysis. this is the first 3d structure solved for any adenovirus that is not a mastadenovirus, allowing cross-genus comparisons between structures and the assignment of genus-specific capsid proteins. viable oadv mutants that lacked the genus-specific lh3 and p ... | 2008 | 18508893 |
| th1 cytokines are upregulated by adenoviral vectors in the brains of primed mice. | a major disadvantage of first generation adenoviral vectors for gene therapy in the brain is the immune response they elicit. human adenovirus is a common respiratory virus and earlier exposure to it has important implications for gene therapy. we show that the immune response against e1-deleted adenoviral vectors in the brain is more deleterious in animals previously exposed to the virus. analysis of cytokine mrna revealed enhanced and prolonged upregulation of the th1 proinflammatory cytokines ... | 2008 | 18628662 |
| pcr-sequence characterization of new adenoviruses found in reptiles and the first successful isolation of a lizard adenovirus. | a consensus nested pcr was used to screen diagnostic samples from approximately 70 reptiles for the presence of adenoviruses (adv) in the years 2006-2007. classical virus isolation methods were also used with all samples. after adenoviruses were detected in a group of helodermatid lizards in a danish zoo, a follow-up study was also carried out on lizards from this group (10 mexican beaded lizards and 24 gila monsters) over the period of a year. adenoviruses were detected in a total of 26 lizards ... | 2009 | 18824312 |
| systemic adenovirus infection in sulawesi tortoises (indotestudo forsteni) caused by a novel siadenovirus. | a novel siadenovirus was identified in the sulawesi tortoise (indotestudo forsteni). a group of 105 sulawesi tortoises was obtained by the turtle survival alliance. many of the tortoises were in poor health. clinical signs included anorexia, lethargy, mucosal ulcerations and palatine erosions of the oral cavity, nasal and ocular discharge, and diarrhea. initial diagnostic tests included fecal testing for parasites, complete blood count and plasma biochemical analysis, mycoplasma serology, and po ... | 2009 | 19564489 |
| isolation and identification of duck adenovirus 1 in ducklings with proliferative tracheitis in ontario. | increased mortality was reported in two flocks of muscovy ducklings from two consecutive hatches originating from the same breeder flock. coughing, dyspnea, and gasping were observed in some ducklings between 6 and 11 days of age. opaque white plugs of exudate were seen in the tracheas with some ducklings having multiple tracheal plugs. tracheal and bronchial epithelium was hyperplastic and superficial epithelial cells contained eosinophilic intranuclear viral inclusions. virus particles compati ... | 2009 | 19630243 |
| ovine atadenovirus, a novel and highly immunogenic vector in prime-boost studies of a candidate hiv-1 vaccine. | ovine adenovirus type 7 (oadv) is the prototype member of the genus atadenovirus. no immunity to the virus has so far been detected in human sera. we describe the construction and evaluation of a candidate hiv-1 vaccine based on oadv and its utilisation alone and in combination with plasmid-, human adenovirus type 5 (hadv5; a mastadenovirus)-, and modified vaccinia ankara (mva)-vectored vaccines. all vectors expressed hiva, an immunogen consisting of hiv-1 clade a consensus gag-derived protein c ... | 2009 | 19853074 |
| prevalence of eight bovine viruses in sika deer (cervus nippon yesoensis) in japan. | | 2009 | 20023282 |
| viral multiplex quantitative pcr assays for tracking sources of fecal contamination. | human and animal fecal pollution of the environment presents a risk to human health because of the presence of pathogenic viruses and bacteria. to distinguish between human and animal sources of pollution, we designed specific real-time reverse transcription (rt)-pcr assays for human and animal enteric viruses, including norovirus genogroups i, ii, and iii; porcine adenovirus types 3 and 5; ovine adenovirus; atadenovirus; and human adenovirus species c and f, which are excreted by infected human ... | 2010 | 20061455 |
| novel recombinant mycobacterium bovis bcg, ovine atadenovirus, and modified vaccinia virus ankara vaccines combine to induce robust human immunodeficiency virus-specific cd4 and cd8 t-cell responses in rhesus macaques. | mycobacterium bovis bacillus calmette-guérin (bcg), which elicits a degree of protective immunity against tuberculosis, is the most widely used vaccine in the world. due to its persistence and immunogenicity, bcg has been proposed as a vector for vaccines against other infections, including hiv-1. bcg has a very good safety record, although it can cause disseminated disease in immunocompromised individuals. here, we constructed a recombinant bcg vector expressing hiv-1 clade a-derived immunogen ... | 2010 | 20375158 |
| induction of both cellular and humoral immunity following a rational prime-boost immunization regimen that incorporates recombinant ovine atadenovirus and fowlpox virus. | recombinant fowlpox viruses (rfpv) and ovine atadenoviruses (roadv) are being developed as safe, nonpathogenic, prophylactic and therapeutic vaccine vectors. there is scope, however, to improve the limited immune responses elicited by each of these vaccine vectors. using previously determined and optimized routes of administration and viral doses, we characterized the primary adaptive immune responses elicited by recombinant variants of each virus. we demonstrate the contrasting nature of the re ... | 2010 | 20810681 |
| efficient replication and generation of recombinant bovine adenovirus-3 in nonbovine cotton rat lung cells expressing i-scei endonuclease. | the rigorous evaluation of recombinant bovine adenovirus (badv)-3 as a gene delivery vector requires quick and efficient method of isolating recombinants. this requires both a suitable cell line and an efficient method of rescuing recombinant badv-3. to facilitate rapid isolation of recombinant badv-3, we have developed an efficient system for generating recombinants using newly identified nonbovine cell line permissive for replication of badv-3. | 2010 | 20963806 |
| a unique novel reptilian paramyxovirus, four atadenovirus types and a reovirus identified in a concurrent infection of a corn snake (pantherophis guttatus) collection in germany. | in 2009, 26 clinical samples (organs and oral/cloacal swabs) from a total of 24 corn snakes (pantherophis guttatus) from a single owner were sent to our laboratory to be tested for the presence of viruses. paramyxoviruses (pmv), adenoviruses (adv) and reoviruses were detected by rt-pcr, pcr and virus isolation methods. three snakes were infected with all three viruses at the same time, while two other snakes had a double infection (pmv and reo, adv and reo) and nine other snakes had a single inf ... | 2011 | 21316873 |
| detection of known and novel adenoviruses in cattle wastes using broad-spectrum primers. | critical assessment of bovine adenoviruses (badv) as indicators of environmental fecal contamination requires improved knowledge of their prevalence, shedding dynamics, and genetic diversity. we examined dna extracted from bovine and other animal waste samples collected in wisconsin for atadenoviruses and mastadenoviruses using novel, broad-spectrum pcr primer sets. badv were detected in 13% of cattle fecal samples, 90% of cattle urine samples and 100% of cattle manure samples; forty-four percen ... | 2011 | 21622778 |
| optimizing hiv-1-specific cd8+ t-cell induction by recombinant bcg in prime-boost regimens with heterologous viral vectors. | the desire to induce hiv-1-specific responses soon after birth to prevent breast milk transmission of hiv-1 led us to propose a vaccine regimen which primes hiv-1-specific t cells using a recombinant mycobacterium bovis bacillus calmette-guérin (rbcg) vaccine. because attenuated live bacterial vaccines are typically not sufficiently immunogenic as stand-alone vaccines, rbcg-primed t cells will likely require boost immunization(s). here, we compared modified danish (aeras-401) and pasteur lysine ... | 2011 | 21932450 |
| Potent antietumor immunity in mice induced by vaccination with an ovine atadenovirus vector. | Identification of adenoviral isolates of nonhuman origin has fostered development of vectors with potential to overcome preexisting immunity in the human population that may affect clinical applications. Ovine adenoviral isolate, OAdV287 (OAdV7), the prototype of the genus Atadenovirus, has been previously characterized as a gene delivery vector although the receptor(s) used for infection remain to be identified. Here, we report the first use of recombinant OAdV7 as a vaccine for inducing an ant ... | 2012 | 22130165 |