| [fauna and ecology of small mammal lice in priamur'e]. | lice occurring on small mammals of priamurje are represented by six species. of 6501 insects collected 91.2% fall on h. acanthopus. 99.8% of lice were collected from rodents of which the main hosts are clethrionomys rufocanus and microtus fortis. the active reproduction of insects takes place in a warm period. high abundance indices were registered from april to july: abundance indices were from 0.6 to 3.0; occurrence indices--from 10.1 to 24.9%. | 1977 | 865884 |
| epidemiological studies on host animals of tsutsugamushi disease in korea. | epidemiological studies on host rodents of tsutsugamushi disease were carried out during the period of july-september 1990 at nine localities of central korea. among total 111 wild rodents trapped by the modified sherman live traps, 103 were apodemus agrarius (92.8%), seven were crocidura lasiura (6.3%) and one was microtus fortis (0.9%), showing 24.0% of trapping rate in winter, 11.7% in spring, 11.2% in summer and 12.0% in autumn. out of 103 a. agrarius 84 were parasitized by chiggers, showing ... | 1991 | 1954201 |
| [the role of hantaan virus serotypes in the etiology of hemorrhagic fever with renal syndrome in the far east of the ussr]. | the employment of two serological tests (indirect fluorescence antibody technique and neutralization test) demonstrated the leading role of hantaan virus serotype 1 strains isolated from field mice in the pattern of the incidence of hemorrhagic fever with renal syndrome (hfrs). proofs of the etiological importance of strains of serotypes 3 and 5 occurring in brown rats and cl. rufocanus were obtained. no data on any association of human hfrs cases with strains of serotype 4 isolated from reed vo ... | 1990 | 1982032 |
| [immunologic monitoring of leptospirosis in the maritime territory]. | the epidemiological and epizootic situation in leptospira infections at the maritime [correction of primorski] territory is evaluated on the basis of complex studies carried out in 1984-1989. as revealed in these studies, cases of leptospirosis among humans have a sporadic character and are mainly registered among professional high risk groups of the population. in the immunological structure of persons covered by the survey l. hebdomadis, l. pomona and l. javanica prevail. the anthropourgic foc ... | 1990 | 2256398 |
| khabarovsk virus: a phylogenetically and serologically distinct hantavirus isolated from microtus fortis trapped in far-east russia. | two hantavirus strains, mf43 and mf113, isolated from microtus fortis trapped in the khabarovsk region of far-eastern russia, were analysed by direct nucleotide sequencing of pcr generated fragments of the m and s segments, by immunofluorescence and by focus reduction neutralization tests (frnt). the nucleotide sequences revealed that the two isolates were closely related to each other but distinct from all other hantaviruses. phylogenetic analysis of the m and s segments showed that the mf stra ... | 1996 | 8627257 |
| [further study of hantavirus circulation in the russian federation]. | lung specimens of 1514 small mammals of 35 species captured in 1991-1995 at 9 territories of russia were tested in elisa for virus antigens of hemorrhagic fever with the renal syndrome (hfrs). the antigens were detected in lung specimens of clethrionomys glareolus, microtus arvalis, microtus gregalis, microtus fortis, arvicola terrestris, apodemus agrarius, micromys minutus, and sorex sp., well known as hantavirus reservoirs, captured in the vologda, yaroslavl, saratov, astrakhan, and chita regi ... | 1997 | 9182404 |
| community structure and prevalence of hantavirus infection in rodents: a geographic division of the enzootic area in far eastern russia. | the results of an extensive rodent trapping effort throughout the southern part of far eastern russia and hantavirus antigen screening of tissues were used to develop a multifaceted approach for the geographic division of the enzootic territory of hantavirus. four species of rodents (apodemus agrarius, apodemus peninsulae, microtus fortis, and clethrionomys rufocanus) comprised 88.5 percent of 10,595 captured rodents and 94.1 percent of 996 antigen-positive animals. rodent fauna and the prevalen ... | 1997 | 9221739 |
| detection of rickettsia sibirica in ticks and small mammals collected in three different regions of china. | the primers rr 190.70p and rr 190.602n were used to detect spotted fever group (sfg) rickettsiae by polymerase chain reaction (pcr) in ticks and small mammals collected in three different regions of china. the obtained results indicated that specific dna fragments of sfg rickettsiae were amplified from dermacentor silvarum, d. sinicus, d. auratus, haemaphysalis concinna, h. wellingtoni, h. yeni, apodemus agrarius, microtus fortis. clethrionomys rufocanus, ondatra zibethica, rattus flavipectus an ... | 1998 | 9645245 |
| [detection of north-asia tick-borne spotted fever in ticks and rodents along the heilongjiang river-side by restriction fragment length polymorphism of pcr products]. | in order to elucidate the natural foci of north-asia tick-borne spotted fever along the bank of heilongjiang river, we used pcr/rflp to detect spotted fever group rickettsiae in ticks and rodents. the results showed that the wild samples of dermacentor silvarum, haemaphysalis concinna and apodemus agrarius, microtus fortis, clethrionomys rufocanus and ondatra zibethica were all positive with amplification, but typhus rickettsiae, tsutsugamushi fever rickettsiae and q fever rickettsiae were all n ... | 1997 | 9812471 |
| genetic diversities of hantaviruses among rodents in hokkaido, japan and far east russia. | seroepizootiologic surveys among wild rodents were carried out in japan and far east russia in 1995 and 1996. seropositive animals were only identified in clethrionomys rufocanus (23/134) in hokkaido, japan. on the other hand, seropositives were identified in c. rufocanus (1/8), apodemus agrarius (2/66), apodemus spp. (2/26) and microtus fortis (3/22) in vladivostok, far east russia. total rna was isolated from lungs of seropositive animals and the s genome segments were amplified by pcr, cloned ... | 1999 | 10082393 |
| natural antibodies in microtus fortis react with antigens derived from four stages in the life-cycle of schistosoma japonicum. | the levels of antibodies which react with the cercarial antigens (ca), schistosomulum stage antigens (ssa), adult-worm antigens (awa) and soluble egg antigens (sea) of schistosoma japonicum were investigated in microtus fortis and albino mice, using an indirect elisa. the m. fortis studied fell into three groups: animals caught in the wild; laboratory-bred animals left unchallenged; and laboratory-bred animals that had been challenged with s. japonicum (30 cercariae/animal) 15 days previously. t ... | 1999 | 10492675 |
| immunological characteristics of natural resistance in microtus fortis to infection with schistosoma japonicum. | to explore the immunological characteristics of natural resistance to schistosoma japonicum infection in microtus fortis (mf) living in the dongting lake area. | 1999 | 11601263 |
| [preliminary report on immunoscreening of schistosoma japonicum adult worm cdna library by sera from infected microtus fortis]. | microtus fortis (mf) is resistant to schistosoma japonicum (sj) infection. to explore if mf responses to special sj antigens after infection, sj adult worm cdna library was screened with sera from infected mf. twelve positive clones were obtained after rescreening. after automatically excised with the helper phage, positive clones were further identified by pcr. the size of sj cdna fragments in positive clones ranged from 300 bp to 1.8 kb. six of these fragments were 300 bp, one was 1 kb, and fi ... | 2000 | 12212194 |
| [preliminary study on the levels of natural antibodies against schistosoma japonicum in microtus fortis in dongting lake area]. | to explore the detection rate and the levels of natural antibodies to cercarial antigen(ca), juvenile antigen(ja), adult worm antigen (awa) and soluble egg antigen (sea), respectively, derived from schistosoma japoncum in microtus fortis distributed in dongting lake area. | 1999 | 12563827 |
| [killing effect of schistosomula of schistosoma japonicum by tissues/organs of microtus fortis in vitro]. | to screen the specific tissues/organs of microtus fortis (mf) that have the activity of anti-schistosomula of schistosoma japonicum. | 2002 | 12575289 |
| partial protection induced by phage library-selected peptides mimicking epitopes of schistosoma japonicum. | to obtain peptide mimicking epitopes of schistosoma japonicum (s. japonicum) through screening of a phage peptide library and to test their potential for induction of protection. | 2003 | 12667407 |
| studies on the parasitic helminths of korea: iii. nematodes and cestodes of rodents. | a survey for nematode and cestode parasites of rodents in korea has been carried out at the areas of chulwon, kumwha, pochon, paju and chungpyong, kyunggi-do. a total of 300 rodents of seven species was examined, comprising of 219 a. agrarius, 33 r. norvegicus, 8 r. alexandrinus, 14 mus musculus yamashinai, microtus fortis pellceus, 21 crocidura russula and a cricetulus trition nester. the following fifteen species belonging to thirteen genera were identified. nematoda: heterakis spumosa, syphac ... | 1968 | 12913546 |
| [comparison of specific genomic dna fragment between microtus fortis calamorum and microtus fortis fortis]. | microtus fortis(taxonomy id: 100897), also named as reed vole, is classified as microtus, micotinae, cricetidae, rodentia, mammalia on taxonomy. microtus fortis mainly distributes in china. some areas of russia, north korea and mongolia close to northeast borderland of china also have a small number of microtus fortis in distribution. microtus fortis in china has principally 4 subspecies, and most of them live is the drainage area of yangtse river. schistosoma japonicum (one of commonly parasite ... | 2003 | 12939799 |
| [serologic type of leptospira isolated from microtus fortis pellicens]. | | 1956 | 13393680 |
| recovery of rickettsia akari from the korean vole microtus fortis pelliceus. | | 1957 | 13478580 |
| [cloning, expressing and functional analysis of sjmf4, a novel schistosoma japonicum gene]. | based on the phenomenon of the natural anti-schistosomiasis in microtus fortis, the sera from normal microtus fortis were employed to immunoscreen the cdna library of adult schistosoma japonicum (chinese strain), two positive clones were obtained, race technique was further applied to amplify one of the clones, and a cdna fragment with an orf was identified. sequencing revealed that it was a novel gene of schistosoma japonicum, and it was named sjmf4 (schistosoma japonicum microtus fortis 4). th ... | 2003 | 14673501 |
| serologic prevalence of toxoplasma gondii in field mice, microtus fortis, from yuanjiang, hunan province, people's republic of china. | antibodies to toxoplasma gondii were investigated in serum samples of field mice, microtus fortis, from yuanjiang, hunan province, people's republic of china. the modified agglutination test (mat) incorporating formalin-fixed whole tachyzoites and mercaptoethanol was used to determine antibodies. antibodies to t. gondii (mat > or = 1:20) were found in 36 (29%) of 124 trapped mice. the antibody titers of positive sera (percentage in parentheses) were 1:20 (8.9), 1:40 (3.2), 1:80 (3.2), 1:160 (1.6 ... | 2004 | 15165081 |
| characterization of a novel vaccine candidate and serine proteinase inhibitor from schistosoma japonicum (sj serpin). | serine proteinase inhibitors (serpins) represent an important superfamily of endogenous inhibitors that regulate proteolytic events active in a variety of physiological functions. immunological screening of a schistosoma japonicum adult worm cdna expression library with sera of microtus fortis, a naturally resistant vertebrate host, has identified one clone that encoded for a sequence homologous to those of the serpin superfamily. the full-length sequence encoding s. japonicum serpin (sj serpin) ... | 2005 | 15946799 |
| molecular screening of bartonella species in rodents from the russian far east. | a potentially broad-range pcr system for glta gene amplification has been designed. a nearly full (992 bp) sequence of the glta gene was amplified in two steps. dna was extracted from organs (spleen and liver) of four mostly spread species of rodents: apodemus agrarius and apodemus peninsulae mice; clethrionomys rufocanus and microtus fortis voles. all amplicons have been sequenced. the incidence of bartonella species in rodents was 73%, 60%, 60%, and 83% for the above-mentioned rodents, respect ... | 2005 | 16481532 |
| [in vitro killing activity of the fractional proteins from microtus fortis serum to schistosoma japonicum juveniles]. | to explore the killing effect of different fractional proteins from microtus fortis (mf) serum to s. japonicum juveniles, and to find possible association of the proteins with the natural resistance to schistosome infection. | 2005 | 16566206 |
| [karyological and allozyme variability of far eastern voles microtus fortis büchner, 1889 (cricetidae, rodentia) from the russian far east]. | karyological (g-, c-, and nor-banding) and allozyme analyses were carried out for island and continental populations of the vole microtus fortis from the far east of russia. variability of the autosome pair 7 was found. the presence of variation in the number of telomere heterochromatin blocks in the populations of far eastern voles was confirmed. nor-staining of the far eastern vole chromosomes was carried out, showing stability of the number and the positions of the nucleolus organizer regions ... | 2006 | 16871789 |
| genetic analysis of hantaviruses carried by myodes and microtus rodents in buryatia. | hantavirus genome sequences were recovered from tissue samples of myodes rufocanus, microtus fortis and microtus oeconomus captured in the baikal area of buryatia, russian federation. genetic analysis of s- and m-segment sequences of buryatian hantavirus strains showed that myodes-associated strains belong to hokkaido virus (hokv) type while microtus-associated strains belong to vladivostok virus (vlav) type. on phylogenetic trees buryatian hokv strains were clustered together with m. rufocanus- ... | 2008 | 18190679 |
| isolation and genetic characterization of hantaviruses carried by microtus voles in china. | to gain more insights into hantavirus distribution in china, microtus fortis were caught in jilin province and m. maximowiczii in the inner mongolia autonomous region. hantavirus specific rna was detected by rt-pcr in 3 out of 26 m. fortis and 5 out of 64 m. maximowiczii. two hantaviruses (fusong-mf-682 and yakeshi-mm-59) were isolated successfully in cell culture and their s and m segment nucleotide sequences were determined. phylogenetic analysis of the s and m segment sequences revealed that ... | 2008 | 18297708 |
| [effect of igg3 antibody purified from sera of microtus fortis against schistosoma japonicum]. | igg3 antibody reaction to soluble antigens prepared from schistosomula (ssa), adult worms (sawa) and eggs (sea) in laboratory-bred microtus fortis (mf), balb/c mice and kunming (km) mice challenged by cercariae of schistosoma japonicum was detected by indirect elisa. the effect of purified igg3 antibody on in vitro killing schistosomula and protecting mice from infection of s. japonicum was evaluated. the igg3 antibody level in mf against ssa and sawa increased significantly by 79.6 percent and ... | 2008 | 18637582 |
| genetic analysis of hantaviruses carried by reed voles microtus fortis in china. | to gain more insights into the epidemiology of hantaviruses in china, the hantaviral s, m and l segment sequences were recovered from two microtus fortis captured in shenyang and four m. fortis trapped in yuanjiang in china. genetic analysis revealed that shenyang sequences are closely related to the sequences of fusong strains of vladivostok virus (vlav). interestingly, the complete s segment and partial l segment sequences from yuanjiang were quite distinct from those of shenyang and fusong st ... | 2008 | 18644410 |
| [screening of t7 phage-display cdna library from liver of microtus fortis with extracts of schistosomulum and characterization of the positive clones]. | microtus fortis is naturally resisitent to schistosoma japonicum. in order to find schistosome-resistence-related genes of microtus fortis, a t7 phage-display cdna library from liver of microtus fortis was screened with the soluble lysate of schistosomula. the specific phages were enriched 375-fold after 3 rounds of biopanning. ninety-two positive clones picked at random were sequenced and 19 ests including 6 unreported genes were obtained. compared with the negative phage clone control, five po ... | 2008 | 18724690 |
| ecological surveillance of small mammals at firing points 10 and 60, gyeonggi province, republic of korea, 2001-2005. | throughout korea, small mammals are hosts to a number of disease-causing agents that pose a health threat to u.s. and korean military forces while they conduct field-training exercises. a seasonal rodent-borne disease surveillance program was established at two firing points (fp), fp-10, and fp-60, and conducted over five years from 2001 through 2005 in response to hantavirus cases among u.s. soldiers. the ecology of these sites consisted primarily of tall grasses associated with semi-permanent ... | 2008 | 19263858 |
| detection of rickettsia typhi and seasonal prevalence of fleas collected from small mammals in the republic of korea. | fleas were collected from live-captured small mammals to identify potential flea-borne pathogens, seasonal prevalence of flea species, and host preference as part of the us military rodent-borne diseases surveillance program conducted at one us military installation and 10 military training sites, northern gyeonggi province, republic of korea. during 2003-04, 948 fleas (563 females and 385 males) were recovered from 2,742 small mammals (seven rodent and one insectivore species). apodemus agrariu ... | 2010 | 20090029 |
| detection of bartonella species in small mammals from zhejiang province, china. | to estimate the prevalence of bartonella in small mammals of different species, during different seasons, and at different study sites, and to provide baseline data for the risk assessment of human bartonella infection, we captured small mammals using snap traps in zhejiang province, china. bartonella species were detected in small-mammal samples by polymerase chain reaction and positive amplicons were sequenced. bartonella dna was detected in 47% (90/192) of apodemus agrarius, 31% (14/45) of ra ... | 2010 | 20090031 |
| identification of the resistance of a novel molecule heat shock protein 90alpha (hsp90alpha) in microtus fortis to schistosoma japonicum infection. | microtus fortis is a naturally resistant vertebrate host of schistosoma japonicum by preventing completion of parasite's life cycle. sera of m. fortis were found to have anti-schistosome effect in vitro and in vivo. in order to identify genes associated with the anti-schistosome effect of m. fortis, we screened a m. fortis marrow cdna expression library by expression cloning and identified a 331-bp clone gc14.75. it was the homologue of heat shock protein 90alpha (hsp90alpha). full-length of m. ... | 2010 | 20347650 |
| molecular cloning and characterization of a gene encoding methionine aminopeptidase 2 of schistosoma japonicum. | methionine aminopeptidase 2 (map2) is an essential enzyme that is involved in protein maturation. it plays a key role in the removal of the initiating methionine residue from nascent polypeptide chains. in the present study, a gene encoding methionine aminopeptidase 2 of schistosoma japonicum (sjmap2) was cloned and characterized. real-time rt-pcr and western blot analysis revealed that this was expressed in each testing developmental stage in s. japonicum, but more highly expressed at 42 days i ... | 2010 | 20582436 |
| serosurveillance of scrub typhus in small mammals collected from military training sites near the dmz, northern gyeonggi-do, korea, and analysis of the relative abundance of chiggers from mammals examined. | comprehensive quarterly serosurveillance on scrub typhus in small mammals collected from military training sites located near the demilitarized zone (dmz), northern gyeonggi-do (province), rok was conducted to determine the potential rodent-borne and associated ectoparasite disease risks to military personnel. a total of 1,196 rodents and insectivores representing 8 species, apodemus agrarius (87.3%, n = 1,044), mus musculus (5.4%, n = 65), crocidura lasiura (3.3%, n = 40), microtus fortis (2.6% ... | 2010 | 20877503 |
| study on differences in the pathology, t cell subsets and gene expression in susceptible and non-susceptible hosts infected with schistosoma japonicum. | more than 40 kinds of mammals in china are known to be naturally infected with schistosoma japonicum (s. japonicum); microtus fortis (m. fortis), a species of vole, is the only mammal in which the schistosomes cannot mature or cause significant pathogenic changes. in the current study, we compared the differences in pathology by hematoxylin-eosin staining and in changes in the t cell subsets with flow cytometry as well as gene expression using genome oligonucleotide microarrays in the lung and l ... | 2010 | 20976156 |
| proteomic analysis of schistosoma japonicum schistosomulum proteins that are differentially expressed among hosts differing in their susceptibility to the infection. | schistosomiasis is a tropical, parasitic disease affecting humans and several animal species. the aim of this study was to identify proteins involved in the growth and survival of the parasitic forms inside a host. schistosomula of schistosoma japonicum were isolated from three different hosts: the susceptible balb/c mice; the wistar rats, which have a considerably lower susceptibility; and the resistant reed vole, microtus fortis. soluble proteins of the schistosomula collected from the above t ... | 2011 | 21593212 |
| detection of rickettsia felis and rickettsia typhi and seasonal prevalence of fleas collected from small mammals at gyeonggi province in the republic of korea. | abstract fleas were collected from live-captured small mammals to identify flea-borne pathogens, host associations, and seasonal prevalence of flea species, as part of the 65th medical brigade rodent-borne disease surveillance program at 20 military installations and training sites, gyeonggi province, republic of korea, 2005-2007. a total of 1251 fleas were recovered from 2833 small mammals. apodemus agrarius, the striped field mouse, accounted for 93.1% (2,637/2,833) of all small mammals captur ... | 2011 | 21612536 |
| karyopherin alpha 2 (kpna2) is associated with the natural resistance to schistosoma japanicum infection in microtus fortis. | microtus fortis is a naturally vertebrate host resistant to schistosoma japonicum infection. in order to understand the molecular mechanism and identify the molecules related to the natural resistance to s. japanicum infection of m. fortis, we screened a gene pool named ge76 by expression cloning and proved it to have high anti-schistosomula effects in our previous work. in this study we identified a clone named ge76.44. we found that the conditioned medium of pcdna1.1-ge76.44 caused 14.0% schis ... | 2011 | 21658898 |
| apoptosis governs the elimination of schistosoma japonicum from the non-permissive host microtus fortis. | the reed vole, microtus fortis, is the only known mammalian host in which schistosomes of schistosoma japonicum are unable to mature and cause significant pathogenesis. however, little is known about how schistosoma japonicum maturation (and, therefore, the development of schistosomiasis) is prevented in m. fortis. in the present study, the ultrastructure of 10 days post infection schistosomula from balb/c mice and m. fortis were first compared using scanning electron microscopy and transmission ... | 2011 | 21731652 |
| [preliminary study on the immunological characteristics of permissive and non-permissive hosts infected with schistosoma japonicum]. | to study the difference among immune responses of three kinds of experimental animals with different susceptibility to the infection of schistosoma japonicum, and preliminarily explore the mechanism of the immune response in permissive and non-permissive hosts. | 2011 | 21972600 |
| Hantaan virus surveillance targeting small mammals at Dagmar North Training Area, Gyeonggi Province, Republic of Korea, 2001-2005. | In response to a hemorrhagic fever with renal syndrome case in November 2000, a seasonal rodent-borne disease surveillance program was initiated at Dagmar North Training Area (DNTA), Gyeonggi Province, Republic of Korea. From April 2001-December 2005, 1,848 small mammals were captured. Apodemus agrarius accounted for 92.5%, followed by Mus musculus (3.6%), Crocidura lasiura (2.1%), and Microtus fortis (1.1%). Three species of rodents were found to be antibody-positive (Ab+) for Hantaan virus (HT ... | 2011 | 22129409 |
| [isolation and culture of lung fibroblasts from microtus fortis and its killing effect on schistosomula in vitro]. | to establish the technique of isolation and culture of lung fibroblasts from microtus fortis in vitro, and observe the killing effect of lung fibroblasts on schistosomula. | 2012 | 23484259 |
| prevalence and molecular characterizations of toxoplasma gondii and babesia microti from small mammals captured in gyeonggi and gangwon provinces, republic of korea. | a survey was conducted to determine the distribution and prevalence of toxoplasma gondii and babesia microti infections in small mammals captured in gyeonggi and gangwon provinces, republic of korea (rok). the serological prevalence of t. gondii (elisa) and b. microti (ifat) was 2.3% (15/667) and 2.1% (14/667), respectively. dna extracts from small mammal heart tissues were screened by pcr for t. gondii and b. microti targeting regions of the gra5 gene and the 18s rrna and β-tubulin genes, respe ... | 2014 | 25178555 |
| hantaan virus surveillance targeting small mammals at nightmare range, a high elevation military training area, gyeonggi province, republic of korea. | rodent-borne disease surveillance was conducted at nightmare range (nm-r), near the demilitarized zone in northeast gyeonggi province, republic of korea, to identify hemorrhagic fever with renal syndrome (hfrs) risks for a mountainous high-elevation (500 m) military training site. monthly surveys were conducted from january 2008-december 2009. a total of 1,720 small mammals were captured belonging to the orders rodentia [families, sciuridae (1 species) and muridae (7 species)] and soricomorpha [ ... | 2015 | 25874643 |
| intestinal nematodes from small mammals captured near the demilitarized zone, gyeonggi province, republic of korea. | a total of 1,708 small mammals (1,617 rodents and 91 soricomorphs), including apodemus agrarius (n = 1,400), microtus fortis (167), crocidura lasiura (91), mus musculus (32), myodes (= eothenomys) regulus (9), micromys minutus (6), and tscherskia (= cricetulus) triton (3), were live-trapped at us/republic of korea (rok) military training sites near the demilitarized zone (dmz) of paju, pocheon, and yeoncheon, gyeonggi province from december 2004 to december 2009. small mammals were examined for ... | 2015 | 25748722 |
| comparative analysis of microrna in schistosomula isolated from non-permissive host and susceptible host. | the reed vole microtus fortis is the only known mammal in which the schistosome is naturally prevented from maturing and schistosome infection does not cause significant pathogenesis. however, the mechanism behind this phenomenon remains unknown. in the present study, solexa deep sequencing technology was used to carry out high-throughput sequencing and comparative analysis of microrna (mirna) between small rna libraries isolated from 10 days oldschistosomula of m. fortis and balb/c mice.in tota ... | 2015 | 26844643 |
| immunoproteomic analysis of schistosoma japonicum schistosomulum proteins recognized by immunoglobulin g in the sera of susceptible and non-susceptible hosts. | the aim of this study was to search for immunogenic schistosomula proteins in the hope of identifying novel intervention targets. schistosomula proteins were analyzed by immunoproteomic which the probes were sera derived from balb/c mice (susceptible hosts) and microtus fortis (resistant hosts). a total of 116 immunoreactive proteins recognized by 10 days post-infected balb/c mice, m. fortis sera, and uninfected m. fortis sera were selected for further analysis. finally, 95 protein spots were id ... | 2015 | 25896740 |
| de novo assembly and transcriptome characterization: novel insights into the natural resistance mechanisms of microtus fortis against schistosoma japonicum. | microtus fortis is a non-permissive host of schistosoma japonicum. it has natural resistance against schistosomes, although the precise resistance mechanisms remain unclear. the paucity of genetic information for m. fortis limits the use of available immunological methods. thus, studies based on high-throughput sequencing technologies are required to obtain information about resistance mechanisms against s. japonicum. | 2014 | 24886088 |
| effects of microtus fortis lymphocytes on schistosoma japonicum in a bone marrow transplantation model. | microtus fortis is a non-permissive host for schistosoma japonicum. while m. fortis lymphocytes are known to provide natural resistance against s. japonicum, the specific mechanism remains unclear. a bone marrow transplantation (bmt) model was established using immunodeficient mice, either nude (experiment 1) or v(d)j recombination activation gene deficient mice (rag-1(-/-)) (experiment 2) as recipients and m. fortis or c57bl/6 mice as donors. the growth and development of s. japonicum were eval ... | 2014 | 24746640 |
| differential expression of micrornas in the non-permissive schistosome host microtus fortis under schistosome infection. | the reed vole microtus fortis is the only mammal known in china in which the growth, development and maturation of schistosomes (schistosoma japonicum) is prevented. it might be that the anti-schistosomiasis mechanisms of m. fortis associate with microrna-mediated gene expression, given that the latter has been found to be involved in gene regulation in eukaryotes. in the present study, the difference between pathological changes in tissues of m. fortis and of mice (mus musculus) post-schistosom ... | 2013 | 24391986 |
| a microtus fortis protein, serum albumin, is a novel inhibitor of schistosoma japonicum schistosomula. | schistosomiasis is an endemic parasite disease and praziquantel is the only drug currently in use to control this disease. experimental and epidemiological evidence strongly suggests that microtus fortis ( mf ) is a naturally resistant vertebrate host of schistosoma japonicum . in the present study, we found that mf serum albumin ( mf -albumin) and the conditioned medium of pcdna3.1- mf -albumin caused 46.2% and 38.7% schistosomula death rates in 96 h, respectively, which were significantly high ... | 2013 | 24271043 |
| [study of closed colony of microtus fortis infected with schistosoma japonicum]. | to understand the pathological changes of tissues and organs in microtus fortis infected with schistosoma japonicum for further insight into the biological dynamic variation and explore immune mechanisms of m. fortis against s. japonicum infection. | 2013 | 24024439 |
| prevalence of schistosoma japonicum in wild rodents in five islands of the west dongting lake, china. | to estimate the prevalence of schistosoma japonicum in field rodents, we conducted surveys of s. japonicum infection on 5 islands in the west dongting lake, china: julinyuan, jiangjiazui, longwangmiao, goutouzhou, and niujiaojian. thirty-five rattus flavipectus, 43 rattus norvegicus, 42 rattus rattus , and 48 microtus fortis were captured and examined from july to august 2011. schistosoma japonicum was found in the 3 rat species but not in the vole m. fortis . no statistically significant differ ... | 2013 | 23259921 |
| characterization of schistosoma japonicum estrogen-related receptor beta like 1 and immunogenicity analysis of the recombinant protein. | the estrogen-related receptor beta like 1 (esrrbl1) is a sex hormone receptor. here, we describe the cloning and expression of the esrrbl1 gene from schistosoma japonicum (sjesrrbl1). quantitative real time pcr (qpcr) and western blot analysis revealed that sjesrrbl1 was highly expressed in 14-, 18-, 23- and 28-days-old schistosomes at the transcriptional and protein levels, when the schistosomes were undergoing early development of reproductive organs, male and female coupling, and egg-laying. ... | 2012 | 22626519 |
| molecular cloning and characterization of a cyclophilin a homologue from schistosoma japonicum. | cyclophilins belong to a group of proteins that have peptidyl-prolyl cis-trans isomerase activity and have been identified in all cell types and all organisms studied. in both prokaryotes and eukaryotes, they have been characterized as functional chaperones and involved in cell signaling. in the present study, sj cyclophilin a (cypa) was cloned, characterized, and subcloned into a prokaryotic expression vector to produce soluble recombinant rsjcypa protein. qpcr analysis revealed that sjcypa was ... | 2012 | 22526287 |
| immune changes of schistosoma japonicum infections in various rodent disease models. | rodent models for schistosoma japonicum infections have demonstrated that these animals possess a degree of resistance to schistosome infections that may be both t and b lymphocyte-mediated. however, their exact role is not well-defined and other immune mechanisms are likely to also play a role in protecting against infection. immunosuppressed and immunocompetent reed voles (microtus fortis, mf), rats and mice (n=24/group) were infected with s. japonicum, and animals were sacrificed 42 days late ... | 2012 | 22521591 |
| first report of genotyping of toxoplasma gondii in free-living microtus fortis in northeastern china. | toxoplasma gondii is an intracellular protozoan parasite which imperils the health of almost all warm-blooded animals, including humans. the objective of this study was to determine genetic characterization of t. gondii in free-living microtus fortis (reed vole) in jilin province, northeastern china. a total of 104 dna samples, 74 from gongzhuling and 30 from baicheng, were extracted from lung tissues of m. fortis , and 56 (53.8%) of them were positive for t. gondii by semi-nested polymerase cha ... | 2014 | 24684624 |
| high prevalence of toxoplasma gondii infection in microtus fortis by seminested pcr from jilin province, northeastern china. | toxoplasma gondii is an important protozoan parasite that can infect virtually all warm-blooded animals, including humans. oocysts in the environment are an important infection source. microtus fortis can be used as a sentinel reflecting the distribution of t. gondii oocysts in the environment indirectly. in the present study, we developed a seminested polymerase chain reaction (pcr) targeting t. gondii 529-base-pair repeat sequence, with high sensitivity and specificity. two hundred seventy-two ... | 2013 | 23050695 |
| two novel ail-positive biotype 1a strains of yersinia enterocolitica isolated in china with unequal adhesion and invasion properties. | yersinia enterocolitica is an enteric pathogen having six biotypes: 1a, 1b, 2, 3, 4, and 5. different bioserotypes have been associated with varying pathogenicity, and the strains of biotype 1a lack the virulence-associated pyv-bearing genes and were once considered to be avirulent. however, there is growing epidemiological, clinical, and experimental evidence to suggest some biotype 1a isolates are virulent and can cause gastrointestinal disease. here, we describe two biotype 1a strains discove ... | 2014 | 25038297 |
| small mammal community succession on the beach of dongting lake, china after the three gorges project. | although the three gorges project (tgp) may have affected the population structure and distribution of plant and animal communities, few studies have analyzed the effect of this project on small mammal communities. therefore, the present paper compares the small mammal communities inhabiting the beaches of dongting lake using field investigations spanning a 20-year period, both before and after the tgp was implemented. snap traps were used throughout the census. the results indicate that the tgp ... | 2014 | 24148252 |
| [the infection status of leptospira in rodents on the heixiazi island of heilongjiang province, china,in 2011]. | to study the infection status of leptospira in rodents on heixiazi island heilongjiang province in 2011. | 2013 | 24113098 |
| a small-scale survey of hantavirus in mammals from eastern poland. | samples of 30 dead small mammals each were collected on area 'a' located in eastern poland which is exposed to flooding by the vistula river, and on the area 'b', also located in eastern poland but not exposed to flooding. kidneys and livers of the mammals were examined by the pcr and nested pcr methods for the presence of hantavirus rna. out of 7 species of small mammals examined, the presence of hantaviruses was detected in 4 of them. hantavirus prevalence was low in apodemus agrarius (2.6%), ... | 2013 | 23772576 |
| first report for the seasonal and annual prevalence of flea-borne bartonella from rodents and soricomorphs in the republic of korea. | rodents and soricomorphs are animal hosts of fleas and associated zoonotic microbial pathogens. a total of 4,889 small mammals were collected from gyeonggi and gangwon provinces, republic of korea, from 2008 through 2010, including: apodemus agrarius (4,122, 84.3%), followed by crocidura lasiura (282, 5.8%), microtus fortis (257, 5.3%), myodes regulus (77, 1.6%), micromys minutus (71, 1.5%), mus musculus (63, 1.3%), and 4 other species (17, 0.3%). a total of 1,099 fleas belonging to 10 species a ... | 2013 | 23590324 |
| hantavirus rna prevalence in myomorph rodents on bolshoy ussuriysky island at the sino-russian border. | to understand the distribution and infection status of hantavirus in myomorph rodents on bolshoy ussuriysky island (heixiazi island) at the sino-russian border, and to provide data for the safe development and utilization of bolshoy ussuriysky island. | 2017 | 28678679 |
| hantavirus surveillance and genetic diversity targeting small mammals at camp humphreys, a us military installation and new expansion site, republic of korea. | small mammal surveillance was conducted (2008-2010, 2012) at camp (cp) humphreys, a us army installation and new expansion site, republic of korea (rok), to identify hemorrhagic fever with renal syndrome health threats to us military/civilian populations during its ongoing expansion phase. small mammals were collected using sherman live capture traps and transported to korea university where they were euthanized, tissues removed, and assayed to determine hantavirus igg antibody-positive and hant ... | 2017 | 28448595 |
| chigger mite (acari: trombiculidae) survey of rodents in shandong province, northern china. | chigger mites are parasites of rodents and other vertebrates, invertebrates, and other arthropods, and are the only vectors of scrub typhus, in addition to other zoonoses. therefore, investigating their distribution, diversity, and seasonal abundance is important for public health. rodent surveillance was conducted at 6 districts in shandong province, northern china (114-112°e, 34-38°n), from january to december 2011. overall, 225/286 (78.7%) rodents captured were infested with chigger mites. a ... | 2017 | 29103271 |
| high throughput data analyses of the immune characteristics of microtus fortis infected with schistosoma japonicum. | microtus fortis exhibits natural resistance against schistosoma japonicum, and the parasite cannot grow and develop in m. fortis. extensive research has been carried out, however, the associated mechanism remains unclear. in the present study, we analysed the combined data obtained from a cytokine chip assay, transcriptome, and metabolome. the cytokine profile from c57bl/6 and m. fortis mice was assessed before and after infection. several cytokines increased during the second and third week pos ... | 2017 | 28900150 |
| reproductive characteristics of the yangtze vole (microtus fortis calamorum) under laboratory feeding conditions. | the reproductive characteristics of a laboratory population of the vole microtus fortis calamorum were examined. voles were allowed to breed under laboratory feeding conditions. over a period of 3 months, 61.82% of the 110 vole pairs examined produced 3 or 4 litters. there were 1-9 voles in each litter and the mean litter size was 4.67±0.28 (mean±se). most litters included 3-7 young voles, accounting for 83.62% of all litters. the mean farrowing interval was 25.9 days (range from 19 to 95 days), ... | 2016 | 26617078 |
| [isolation and identification of macrophages from liver of microtus fortis]. | to separate and purify intrahepatic macrophages from microtus fortis (mf) and identify its phagocytosis. | 2015 | 26510361 |
| the first complete mitochondrial genome of the microtus ochrogaster. | microtus ochrogaster, a small vole, found in central north america. recently the genome sequencing had been done, but no more information of its mitochondrial reported. herein, we first assembled the complete mitochondrial genome sequence of microtus ochrogaster. it is a 16 292 bp long sequence with most mitogenome's characteristic structure, 13 protein-coding genes, two of rrna genes, 22 of trna genes, one d-loop region, one repeat region, and three sts regions. the gc-content of our fresh sequ ... | 2016 | 26305486 |
| habitat evaluation for outbreak of yangtze voles (microtus fortis) and management implications. | rodent pests severely damage agricultural crops. outbreak risk models of rodent pests often do not include sufficient information regarding geographic variation. habitat plays an important role in rodent-pest outbreak risk, and more information about the relationship between habitat and crop protection is urgently needed. the goal of the present study was to provide an outbreak risk map for the dongting lake region and to understand the relationship between rodent-pest outbreak variation and hab ... | 2015 | 25316099 |
| effect of population density on reproduction in microtus fortis under laboratory conditions. | between december 2011 and march 2012, the reproductive characteristics of microtus fortis reared in the laboratory at different population densities were assessed. in all, 258 male and female voles were randomly divided into 4 groups and reared at densities of 2, 4, 6, and 8 animals per cage (sex ratio: 1:1). the results showed that the pregnancy rate (χ2 = 21.671, df = 3, p < 0.001) and first farrowing interval (f = 12.355, df = 3, p < 0.001) were significantly different among the different pop ... | 2014 | 24873906 |
| [possible impacts of the three gorges project and converting farmland into lake on the microtus fortis population in dongting lake region]. | the outbreak of yantze vole (microtus fortis) population in dongting lake region is closely related to the evolvement of lake beaches, because the deposition of lake sediments results in the expansion of lake beaches, which provides the possibility of the increase of the vole's population. reclaiming farmland from lake via building cofferdams, eliminating snails by building cofferdams for eradicating schistosomiasis, and over-hunting predators in the region cause the voles becoming a pest after ... | 2012 | 23189685 |
| the complete mitochondrial genome of microtus fortis calamorum (arvicolinae, rodentia) and its phylogenetic analysis. | microtus fortis is a special resource of rodent in china. it is a promising experimental animal model for the study on the mechanism of schistosome japonicum resistance. the first complete mitochondrial genome sequence for microtus fortis calamorum, a subspecies of m. fortis (arvicolinae, rodentia), was reported in this study. the mitochondrial genome sequence of m. f. calamorum (genbank: jf261175) showed a typical vertebrate pattern with 13 protein coding genes, 2 ribosomal rnas, 22 transfer rn ... | 2012 | 22387301 |
| [enrichment and screening of the microsatellite markers in microtus fortis and its preliminary application in genetic diversity research]. | this study was to isolate microsatellite markers from microtus fortis genome by magnetic beads enrichments. through hybridization of biotin-labeled microsatellite oligonucleotide probes, which were captured by streptavidin-coated magnetic with the adaptor-ligated enzyme-digested genome fragments, single-stranded dna fragments containing microsatellites were obtained. after pcr amplification, these fragments were then cloned into t vectors and were transformed into competent cells subsequently. n ... | 2011 | 21951800 |
| effect of enso-driven precipitation on population irruptions of the yangtze vole microtus fortis calamorum in the dongting lake region of china. | the yangtze vole (microtus fortis buechner, 1889) is a small herbivore species that inhabits lake beaches in the dongting lake region along the yangtze river in southern china. its population shows strong oscillations during the wet season due to summer precipitation-induced immigration away from the lake into adjacent rice fields. the effect of el niño-southern oscillation-driven precipitation on population abundance and growth of the vole species is not fully understood. we undertook an analys ... | 2010 | 21392335 |
| [population dynamics of microtus fortis in dongting lake region and its forecasting]. | the population dynamics of microtus fortis in yueyang, hunan province was studied from 1991 to 1996. m. fortis lived on the beach of the lake from early autumn to next spring, but moved to the farmland in summer when the beach was flooded. when lived on the beach, its population increased gradually. usually, m. fortis made great damage on the crops when moved to the farmland. the population dynamics of m. fortis was different on the beach and on the farmland, and the key affecting factors were a ... | 2004 | 15146646 |
| [evaluation on the impacts of environmental changes and three-gorge engineering on the population of yangtze voles (microtus forits) in the dongting lake region]. | the relationships between the population of yangtze voles (microtus fortis) and the evolvement of the dongting lake beaches were analyzed based on historical data, and the impacts of the three-gorge engineering on beach environment and water level of the lake were assessed according to the regulation scheme on downward discharge flux of the three-gorge dam. the results indicated that the population of the voles has been increasing since 1950's, owing to the continuous enlargement of the lake bea ... | 2002 | 12181902 |
| [preparation of recombinant retrovirus prevtre-e77.43 and its protective effect in a mouse model ofschistosoma japonicuminfection]. | to explore the biological functions of e77.43, a gene segment ofmicrotus fortis, in treatingschistosoma japonicuminfection. | 2017 | 29469452 |
| spatial heterogeneity of hemorrhagic fever with renal syndrome is driven by environmental factors and rodent community composition. | hemorrhagic fever with renal syndrome (hfrs) is a rodent-borne disease caused mainly by two hantaviruses in china: hantaan virus and seoul virus. environmental factors can significantly affect the risk of contracting hantavirus infections, primarily through their effects on rodent population dynamics and human-rodent contact. we aimed to clarify the environmental risk factors favoring rodent-to-human transmission to provide scientific evidence for developing effective hfrs prevention and control ... | 2018 | 30356291 |
| mechanisms of resistance to schistosoma japonicum infection in microtus fortis, the natural non-permissive host. | human schistosomiasis, which is caused by schistosomes, is a zoonosis that is difficult to control because of the many reservoir hosts. however, microtus fortis is the only mammal that is naturally resistant to schistosoma japonicum infection known in china, in which s. japonicum growth and development were arrested on day 12, and the worms eliminated on day 20 post-infection. in this review, we present an overview of the established and purported mechanisms of resistance to s. japonicum infecti ... | 2020 | 33013763 |
| a longitudinal study reveals the alterations of the microtus fortis colonic microbiota during the natural resistance to schistosoma japonicum infection. | the gut microbiota has been demonstrated to associate with protection against helminth infection and mediate via microbial effects on the host humoral immunity. as a non-permissive host of schistosoma japonicum, the microtus fortis provides an ideal animal model to be investigated, because of its natural self-healing capability. although researches on the systemic immunological responses have revealed that the host immune system contributes a lot to the resistance, the role of gut microbiome rem ... | 2020 | 33080305 |
| [resistance of raav2-mfe77.43-transferred mice to schistosoma japonicum infection]. | to investigate the resistance of e77.43 gene of microtus fortis（mfe77.43） to schistosoma japonicum infection. | 2016 | 30146861 |
| [culture and characterization of spontaneous ascites cells isolated from microtus fortis]. | to isolate and culture the spontaneous ascites cells from microtus fortis under artificial conditions, so as to investigate the molecular mechanism at the cell level. | 2016 | 29469420 |