| electronic particle counting of zoospores of aphanomyces euteiches. | | 1971 | 5548901 |
| factors affecting growth and development of aphanomyces euteiches. | | 1966 | 5946310 |
| cytological and fine-structure studies of aphanomyces euteiches. | | 1966 | 5946311 |
| studies on zoospore attracting activity. ii. synthesis of isoflavones and their attracting activity to aphanomyces euteiches zoospore. | nineteen isoflavones were synthesized and their attracting activity to aphanomyces euteiches zoospore were investigated. isoflavones (1, 2, 4, 16 and 19) with an unsubstituted b ring were synthesized by the oxidation of corresponding 2'-hydroxychalcones with thallium (iii) nitrate trihydrate in methanolic perchloric acid. a hydroxyl group at the c-5 position in isoflavones was necessary for strong attraction to a. euteiches zoospore. the introduction of an additional hydroxyl group at the c-7 or ... | 1993 | 8401405 |
| is a fully established arbuscular mycorrhizal symbiosis required for a bioprotection of pisum sativum roots against aphanomyces euteiches? | bioprotection of pea roots against aphanomyces euteiches by the arbuscular mycorrhizal fungus g. mosseae was demonstrated to depend on a fully established symbiosis. this was related with induction of mycorrrhiza-related chitinolytic enzymes. possible mechanisms implicated in bioprotection are discussed. | 2000 | 10659715 |
| zoospore homing and infection events: effects of the biocontrol bacterium burkholderia cepacia ammdr1 on two oomycete pathogens of pea (pisum sativum l.). | burkholderia cepacia ammdr1 is a biocontrol agent that protects pea and sweet corn seeds from pythium damping-off in field experiments. the goal of this work was to understand the effect of b. cepacia ammdr1 on pythium aphanidermatum and aphanomyces euteiches zoospore homing events and on infection of pea seeds or roots. in vitro, b. cepacia ammdr1 caused zoospore lysis, prevented cyst germination, and inhibited germ tube growth of both oomycetes. b. cepacia ammdr1 also reduced the attractivenes ... | 2000 | 11097889 |
| combined effects of brassica napus seed meal and trichoderma harzianum on two soilborne plant pathogens. | the effects of soil amendment with rapeseed meal from brassica napus cv. 'dwarf essex' (high glucosinolate concentrations) and 'stonewall' (low glucosinolate concentrations) on the biological control activity of trichoderma harzianum towards sclerotinia sclerotiorum and aphanomyces euteiches were evaluated. trichoderma harzianum added to soil reduced myceliogenic germination of s. sclerotiorum by 94%, but did not affect carpogenic germination. in contrast, 100% reduction in carpogenic germinatio ... | 2000 | 11109495 |
| effects of infection of common root rot on protein content, cooking quality and other characters in pea varieties. | pea varieties are known to show a considerable variability in seed yield, protein content, cooking quality and other characters over years and locations. common root rot, a wide spread soil borne disease of peas in europe and elsewhere, is a contributing factor in the observed variation in pea yields and pea quality characteristics such as protein content and cooking quality. it interacts with the weather and these factors together constitute important reasons for the variations observed within ... | 2001 | 11712233 |
| interactions between indigenous arbuscular mycorrhizal fungi and aphanomyces euteiches in field-grown pea. | this is the first reported study of the interactions between indigenous arbuscular mycorrhizal fungi (amf) and aphanomyces euteiches in pea under field conditions. a. euteiches was applied to the soil by adding oospores produced in vitro. attempts were made to create a non-mycorrhizal control by incorporating carbendazim (derosal fl) in the topsoil before sowing. however, all carbendazim-treated plants showed approximately 20% root colonisation with amf. pea plants not treated with carbendazim s ... | 2002 | 11968948 |
| quantitative trait loci for partial resistance to aphanomyces root rot in pea. | aphanomyces root rot, caused by aphanomyces euteiches drechs, is the most-important disease of pea ( pisum sativum l.) worldwide. no efficient chemicals are available to control the pathogen. to facilitate breeding for aphanomyces root rot resistance and to better understand the inheritance of partial resistance, our goal was to identify qtls associated with field partial resistance. a population of 127 rils from the cross puget (susceptible) x 90-2079 (partially resistant) was used. the lines w ... | 2002 | 12582868 |
| a member of the germin-like protein family is a highly conserved mycorrhiza-specific induced gene. | a medicago truncatula cdna encoding a germin-like protein (glp) was isolated from a suppression subtractive hybridization cdna library enriched for arbuscular mycorrhiza (am)-induced genes. the mtglp1 amino acid sequence shows some striking differences to previously described plant glp sequences and might therefore represent a new subgroup of this multigene family. the mtglp1 mrna was strongly induced in roots and root cultures colonized by the am fungus glomus intraradices. whereas mtglp1 is st ... | 2003 | 14634158 |
| proteomic approach: identification of medicago truncatula proteins induced in roots after infection with the pathogenic oomycete aphanomyces euteiches. | the legume root rot disease caused by the oomycete pathogen aphanomyces euteiches is one major yield reducing factor in legume crop production. a comparative proteomic approach was carried out in order to identify proteins of the model legume medicago truncatula which are regulated after an infection with a. euteiches . several proteins were identified by two dimensional gel electrophoresis to be differentially expressed after pathogen challenge. densitometric evaluation of expression values sho ... | 2004 | 15604668 |
| proteomic profiling unravels insights into the molecular background underlying increased aphanomyces euteiches-tolerance of medicago truncatula. | to investigate the molecular mechanisms underlying susceptibility of legumes to the root pathogen aphanomyces euteiches (oomycota), comparative proteomic studies have been carried out. in a first approach, we have analysed two medicago truncatula lines of the french core collection (f83.005-5 (r2002) and f83.005-9 (r2002)), which showed either increased or decreased susceptibility to a. euteiches as compared to the widely adopted line a17. several proteins were identified to be differentially in ... | 2005 | 16235107 |
| identification of a genetic locus in pseudomonas aureofaciens involved in fungal inhibition. | in iron-rich conditions, pseudomonas aureofaciens pa147-2 produces an antibiotic-like compound that inhibits the growth of a plant fungal pathogen, aphanomyces euteiches. to contribute to the potential use of pa147-2 as a biocontrol organism, we report the identification of a genetic locus important for antibiotic biosynthesis. mutants defective for fungal inhibition (af) were generated by tn5 mutagenesis. southern hybridization of total dnas from three af mutants indicated that loss of fungal i ... | 1994 | 16349166 |
| gene expression analysis of arbuscule development and functioning. | the arbuscular mycorrhiza (am) is characterized by specific morphological structures of the fungus and the plant and by physiological adaptations which are mostly beneficial for both partners of the symbiosis. this review describes approaches to study the molecular basis of the interaction. rna accumulation patterns have been monitored in pisum sativum to analyse the plant response to arbuscule development. in a direct approach, the mtha1 gene from medicago truncatula was cloned which is express ... | 2007 | 17081578 |
| silencing of pr-10-like proteins in medicago truncatula results in an antagonistic induction of other pr proteins and in an increased tolerance upon infection with the oomycete aphanomyces euteiches. | recent studies on the root proteome of medicago truncatula (gaertn.) showed an induction of pathogenesis-related (pr) proteins of the class 10 after infection with the oomycete pathogen aphanomyces euteiches (drechs.). to get insights into the function of these proteins during the parasitic root-microbe association, a gene knockdown approach using rnai was carried out. agrobacterium rhizogenes-mediated transformation of m. truncatula roots led to a knockdown of the medicago pr10-1 gene in transg ... | 2007 | 17237953 |
| development of a molecular method to detect and quantify aphanomyces euteiches in soil. | a real-time pcr assay using 136f/211r primers and 161t taqman probe for the detection and quantification of aphanomyces euteiches in soil is presented. the specificity of primers was tested on 105 different a. euteiches isolates, mainly from france. a calibration curve was established with a plasmid phs1 resulting from the target region cloned into the pcr4 topo vector (invitrogen). the target copy number was evaluated and was constant whatever the isolate. a dna-based method was able to discrim ... | 2007 | 17559389 |
| development of codominant simple sequence repeat, single nucleotide polymorphism and sequence characterized amplified region markers for the pea root rot pathogen, aphanomyces euteiches. | three kinds of genetic markers including simple sequence repeats (ssrs), single nucleotide polymorphisms (snps) and sequence characterized amplified regions (scars) were developed from aphanomyces euteiches. of 69 loci tested, seven ssr, two snp and two scar markers were codominantly polymorphic. these codominant markers and dominant markers described herein will facilitate population genetic and evolutionary studies of this important plant pathogen. | 2007 | 17884212 |
| transcriptome of aphanomyces euteiches: new oomycete putative pathogenicity factors and metabolic pathways. | aphanomyces euteiches is an oomycete pathogen that causes seedling blight and root rot of legumes, such as alfalfa and pea. the genus aphanomyces is phylogenically distinct from well-studied oomycetes such as phytophthora sp., and contains species pathogenic on plants and aquatic animals. to provide the first foray into gene diversity of a. euteiches, two cdna libraries were constructed using mrna extracted from mycelium grown in an artificial liquid medium or in contact to plant roots. a unigen ... | 2008 | 18320043 |
| cell wall chitosaccharides are essential components and exposed patterns of the phytopathogenic oomycete aphanomyces euteiches. | chitin is an essential component of fungal cell walls, where it forms a crystalline scaffold, and chitooligosaccharides derived from it are signaling molecules recognized by the hosts of pathogenic fungi. oomycetes are cellulosic fungus-like microorganisms which most often lack chitin in their cell walls. here we present the first study of the cell wall of the oomycete aphanomyces euteiches, a major parasite of legume plants. biochemical analyses demonstrated the presence of ca. 10% n-acetyl-d-g ... | 2008 | 18806214 |
| consistent quantitative trait loci in pea for partial resistance to aphanomyces euteiches isolates from the united states and france. | abstract development of pea cultivars resistant to aphanomyces root rot, the most destructive root disease of pea worldwide, is a major disease management objective. in a previous study of a mapping population of 127 recombinant inbred lines (rils) derived from the cross 'puget' (susceptible) x '90-2079' (partially resistant), we identified seven genomic regions, including a major quantitative trait locus (qtl), aph1, associated with partial resistance to aphanomyces root rot in u.s. fields (21) ... | 2005 | 18943359 |
| hierarchical analysis of diversity, selfing, and genetic differentiation in populations of the oomycete aphanomyces euteiches. | abstract relatively little is known about the population biology of the legume pathogen aphanomyces euteiches. a. euteiches is a soilborne pathogen causing aphanomyces root rot of several legumes, including alfalfa, bean, lentil, and pea. our objectives were to assess the degree of diversity, selfing, and population differentiation in a. euteiches. we contrasted populations within and among two geographically separated fields with a history of pea production. molecular genotyping relied on ampli ... | 2006 | 18943502 |
| postinfection biological control of oomycete pathogens of pea by burkholderia cepacia ammdr1. | abstract burkholderia cepacia ammdr1 is a biocontrol agent that reduces pythium damping-off and aphanomyces root rot severity on peas in the field. we studied the effect of b. cepacia ammdr1 on post-infection stages in the life cycles of these pathogens, including mycelial colonization of the host, production of oogonia, and production of secondary zoospore inoculum. we used burkholderia cepacia 1324, a seed and rootcolonizing but antibiosis-deficient tn5 mutant of b. cepacia ammdr1, to study me ... | 2001 | 18943851 |
| quantifying aphanomyces euteiches in alfalfa with a fluorescent polymerase chain reaction assay. | abstract a polymerase chain reaction (pcr) assay using a set of specific primers and a dual-labeled probe (taqman) was developed to quantify the amount of aphanomyces euteiches dna in alfalfa plants exhibiting varying levels of disease severity. the study included isolates of race 1 and race 2 of a. euteiches. the assay also discriminated between alfalfa populations for resistance based on analysis of dna extracted from bulked plant samples. analysis of individual plants and bulked plant samples ... | 2002 | 18943997 |
| biological control of pathogens causing root rot complex in field pea using clonostachys rosea strain acm941. | abstract pea root rot complex (prrc), caused by alternaria alternata, aphanomyces euteiches, fusarium oxysporum f. sp. pisi, f. solani f. sp. pisi, mycosphaerella pinodes, pythium spp., rhizoctonia solani, and sclerotinia sclerotiorum, is a major yield-limiting factor for field pea production in canada. a strain of clonostachys rosea (syn. gliocladium roseum), acm941 (atcc 74447), was identified as a mycoparasite against these pathogens. when grown near the pathogen, acm941 often was stimulated ... | 2003 | 18944343 |
| a pcr-based assay by sequence-characterized dna markers for the identification and detection of aphanomyces euteiches. | abstract polymerase chain reaction (pcr) products were identified and amplified from isolates of aphanomyces euteiches and a. cochlioides. the products were cloned and sequenced, and the data were used to design pairs of extended pcr primers to amplify sequence-characterized dna markers. the primer pair opc7-fs-30 and opc7-rs-25 amplified a single 1,332-bp product from all isolates of a. euteiches that were not amplified from any other isolates tested. a single 718-bp product was selectively amp ... | 2000 | 18944478 |
| genotypic and pathogenic diversity among pea-infecting strains of aphanomyces euteiches from the central and western united states. | abstract pathogenic and genotypic variability among four populations of aphanomyces euteiches from individual fields in minnesota, wisconsin, and oregon were investigated using pathogenicity and randomly amplified polymorphic dna (rapd) analyses. about 50 strains were isolated from each of two pea fields in minnesota, and 11 and 6 strains from pea fields in wisconsin and oregon, respectively, using pea (pisum sativum) as a baiting host. pathogenic variability and host range were evaluated in gre ... | 1998 | 18944869 |
| enzymatic activity of the mycelium compared with oospore development during infection of pea roots by aphanomyces euteiches. | abstract to describe the disease cycle of the root pathogen aphanomyces euteiches, enzymatic activity in the mycelium was compared with the development of oospores in pea roots. plants were inoculated with two zoospore concentrations to achieve different disease levels. hyphae were stained for fungal alkaline phosphatase activity in the roots. additionally, enzyme activity was measured after electrophoresis of an a. euteiches-specific glucose-6-phosphate isozyme. development of oospores in the r ... | 1998 | 18944878 |
| variation in pathogenicity and genotype among single-zoospore strains of aphanomyces euteiches. | abstract the role of asexual reproduction in the production of pathogenic and genotypic variation in aphanomyces euteiches was investigated. variation was studied among three groups of 18 single-zoospore progeny of a. euteiches derived from each of three single-zoospore parental strains. pathogenicity was assessed by evaluating disease severity (ds) on roots of five pea lines possessing different levels of resistance to aphanomyces root rot and of a susceptible cultivar of snap bean and alfalfa. ... | 1998 | 18944999 |
| comparison of soil receptivity to thielaviopsis basicola, aphanomyces euteiches, and fusarium solani f. sp. pisi causing root rot in pea. | abstract soil receptivity as a quantifiable characteristic ranging from conduciveness to suppressiveness to soilborne pea pathogens thielaviopsis basicola and aphanomyces euteiches was determined by analysis of differences in disease response curves obtained by artificial introduction of inoculum into natural field soil samples. several parameters, including maximum root rot severity, the area under the health index curve, scores on the first axis of a principal component analysis (pca) on dose ... | 1997 | 18945109 |
| toxicity of glucosinolate degradation products from brassica napus seed meal toward aphanomyces euteiches f. sp. pisi. | abstract brassica tissues are potentially useful in the control of aphanomyces root rot of peas (pisum sativum), but identity of the responsible compounds and specific impacts of those compounds on the pathogen's infection potential remain uncertain. brassica napus seed meals and water extracts from these meals were used to determine the effect of glucosinolate hydrolysis products on aphanomyces euteiches f. sp. pisi. b. napus meal ('dwarf essex') containing glucosinolates and intact myrosinase, ... | 1997 | 18945157 |
| aer1, a major gene conferring resistance to aphanomyces euteiches in medicago truncatula. | aphanomyces euteiches is a major soilborne oomycete pathogen that infects various legume species, including pea and alfalfa. the model legume medicago truncatula has recently emerged as a valuable genetic system for understanding the genetic basis of resistance to a. euteiches in leguminous crops. the objective of this study was to identify genetic determinants of resistance to a broad host-range pea-infecting strain of a. euteiches in m. truncatula. two m. truncatula segregating populations of ... | 2009 | 19159312 |
| phylogenetic relationships among plant and animal parasites, and saprotrophs in aphanomyces (oomycetes). | molecular phylogenetic relationships among 12 species of aphanomyces de bary (oomycetes) were analyzed based on 108 its sequences of nuclear rdna. sequences used in the analyses belonged to the major species currently available in pure culture and genbank. bayesian, maximum likelihood, and maximum parsimony analyses support that aphanomyces constitutes a monophyletic group. three independent lineages were found: (i) plant parasitic, (ii) animal parasitic, and (iii) saprotrophic or opportunistic ... | 2009 | 19236935 |
| induction of distinct defense-associated protein patterns in aphanomyces euteiches (oomycota)-elicited and -inoculated medicago truncatula cell-suspension cultures: a proteome and phosphoproteome approach. | a comprehensive proteomic approach was applied to investigate molecular events occurring upon inoculation of medicago truncatula cell-suspension cultures with the oomycete root pathogen aphanomyces euteiches. establishment of an inoculation assay in the cell cultures allowed a direct comparison between proteins induced by elicitation with a crude culture extract of the oomycete and by inoculation with a. euteiches zoospores representing the natural infection carrier. oxidative burst assays revea ... | 2009 | 19271957 |
| sterol metabolism in the oomycete aphanomyces euteiches, a legume root pathogen. | sterols are isoprenoid-derived molecules that have essential functions in eukaryotes but whose metabolism remains largely unknown in a large number of organisms. oomycetes are fungus-like microorganisms that are evolutionarily related to stramenopile algae, a large group of organisms for which no sterol metabolic pathway has been reported. here, we present data that support a model of sterol biosynthesis in aphanomyces euteiches, an oomycete species causing devastating diseases in legume crops. ... | 2009 | 19496952 |
| partial resistance of medicago truncatula to aphanomyces euteiches is associated with protection of the root stele and is controlled by a major qtl rich in proteasome-related genes. | a pathosystem between aphanomyces euteiches, the causal agent of pea root rot disease, and the model legume medicago truncatula was developed to gain insights into mechanisms involved in resistance to this oomycete. the f83005.5 french accession and the a17-jemalong reference line, susceptible and partially resistant, respectively, to a. euteiches, were selected for further cytological and genetic analyses. microscopy analyses of thin root sections revealed that a major difference between the tw ... | 2009 | 19656040 |
| differential gel electrophoresis (dige) to quantitatively monitor early symbiosis- and pathogenesis-induced changes of the medicago truncatula root proteome. | symbiosis- and pathogenesis-related early protein induction patterns in the model legume medicago truncatula were analysed with two-dimensional differential gel electrophoresis. two symbiotic soil microorganisms (glomus intraradices, sinorhizobium meliloti) were used in single infections and in combination with a secondary pathogenic infection by the oomycete aphanomyces euteiches. proteomic analyses performed 6 and 24h after inoculations led to identification of 87 differentially induced protei ... | 2010 | 19895911 |
| a complex genetic network involving a broad-spectrum locus and strain-specific loci controls resistance to different pathotypes of aphanomyces euteiches in medicago truncatula. | a higher understanding of genetic and genomic bases of partial resistance in plants and their diversity regarding pathogen variability is required for a more durable management of resistance genetic factors in sustainable cropping systems. in this study, we investigated the diversity of genetic factors involved in partial resistance to aphanomyces euteiches, a very damaging pathogen on pea and alfalfa, in medicago truncatula. a mapping population of 178 recombinant inbred lines, from the cross f ... | 2010 | 20012740 |
| sterol biosynthesis in oomycete pathogens. | oomycetes are a diverse group of filamentous eukaryotic microbes comprising devastating animal and plant pathogens. they share many characteristics with fungi, including polarized hyphal extension and production of spores, but phylogenetics studies have clearly placed oomycetes outside the fungal kingdom, in the kingdom stramenopila which also includes marine organisms such as diatoms and brown algae. oomycetes display various specific biochemical features, including sterol metabolism. sterols a ... | 2010 | 20023385 |
| root rot disease of legumes caused by aphanomyces euteiches. | the oomycete genus aphanomyces houses plant and animal pathogens found in both terrestrial and aquatic habitats. aphanomyces euteiches drechs. causes seedling damping off and root rot diseases on many legumes. it is the most devastating pea (pisum sativum) disease in several countries, causing up to 80% losses each year. this strictly soil-borne pathogen may survive many years in soil and no efficient chemical control is currently available. the only way to control the disease is to avoid cultiv ... | 2007 | 20507520 |
| ancient class of translocated oomycete effectors targets the host nucleus. | pathogens use specialized secretion systems and targeting signals to translocate effector proteins inside host cells, a process that is essential for promoting disease and parasitism. however, the amino acid sequences that determine host delivery of eukaryotic pathogen effectors remain mostly unknown. the crinkler (crn) proteins of oomycete plant pathogens, such as the irish potato famine organism phytophthora infestans, are modular proteins with predicted secretion signals and conserved n-termi ... | 2010 | 20847293 |
| new consistent qtl in pea associated with partial resistance to aphanomyces euteiches in multiple french and american environments. | partial resistances, often controlled by quantitative trait loci (qtl), are considered to be more durable than monogenic resistances. therefore, a precursor to developing efficient breeding programs for polygenic resistance to pathogens should be a greater understanding of genetic diversity and stability of resistance qtl in plants. in this study, we deciphered the diversity and stability of resistance qtl to aphanomyces euteiches in pea towards pathogen variability, environments and scoring cri ... | 2011 | 21479935 |
| association between border cell responses and localized root infection by pathogenic aphanomyces euteiches. | background and aims the oomycete aphanomyces euteiches causes up to 80 % crop loss in pea (pisum sativum). aphanomyces euteiches invades the root system leading to a complete arrest of root growth and ultimately to plant death. to date, disease control measures are limited to crop rotation and no resistant pea lines are available. the present study aims to get a deeper understanding of the early oomycete-plant interaction at the tissue and cellular levels. methods here, the process of root infec ... | 2011 | 21807690 |