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early responses in the arabidopsis-verticillium longisporum pathosystem are dependent on ndr1, ja- and et-associated signals via cytosolic npr1 and rfo1.the responses of arabidopsis accessions and characterized genotypes were used to explore components in the early defense responses to the soilborne fungus verticillium longisporum. v. longisporum susceptibility was found to be a complex trait, in which different disease phenotypes, such as stunting, altered flowering time, weight loss, and chlorosis were perceived differently across genotypes. a bay-0 x shahdara recombinant inbred line population was used to identify two loci on chromosomes 2 an ...200616941900
defence reactions in the apoplastic proteome of oilseed rape (brassica napus var. napus) attenuate verticillium longisporum growth but not disease symptoms.verticillium longisporum is one of the most important pathogens of brassicaceae that remains strictly in the xylem during most stages of its development. it has been suggested that disease symptoms are associated with clogging of xylem vessels. the aim of our study was to investigate extracellular defence reactions induced by v. longisporum in the xylem sap and leaf apoplast of brassica napus var. napus in relation to the development of disease symptoms, photosynthesis and nutrient status.200819094241
the plant host brassica napus induces in the pathogen verticillium longisporum the expression of functional catalase peroxidase which is required for the late phase of disease.the devastating soil-borne fungal pathogen verticillium longisporum is host-specific to brassicaceae including oilseed rape (brassica napus) as the economically most important crop. the fungus infects through the roots and causes stunting and early senescence of susceptible host plants and a marked decrease in crop yield. we show here that v. longisporum reacts to the presence of b. napus xylem sap with the production of six distinct up-regulated and eight down-regulated proteins visualized by 2 ...201122112218
expression of bvglp-1 encoding a germin-like protein from sugar beet in arabidopsis thaliana leads to resistance against phytopathogenic fungi.nematode (heterodera schachtii) resistance in sugar beet (beta vulgaris) is controlled by a single dominant resistance gene, hs1(pro-1). bvglp-1 was cloned from resistant sugar beet. the bvglp-1 messenger (m)rna is highly upregulated in the resistant plants after nematode infection, suggesting its role in the hs1(pro-1) mediated resistance. bvglp-1 exhibits sequence homology to a set of plant germin-like proteins (glp), from which several have proved to be functional in plant basal or defense re ...201020192832
silencing of vlaro2 for chorismate synthase revealed that the phytopathogen verticillium longisporum induces the cross-pathway control in the xylem.the first leaky auxotrophic mutant for aromatic amino acids of the near-diploid fungal plant pathogen verticillium longisporum (vl) has been generated. vl enters its host brassica napus through the roots and colonizes the xylem vessels. the xylem contains little nutrients including low concentrations of amino acids. we isolated the gene vlaro2 encoding chorismate synthase by complementation of the corresponding yeast mutant strain. chorismate synthase produces the first branch point intermediate ...201019826808
internal resistance in winter oilseed rape inhibits systemic spread of the vascular pathogen verticillium longisporum.verticillium longisporum is a vascular fungal pathogen presently threatening oilseed rape production in europe. systemic spread and vascular responses were studied in a susceptible ('falcon') and a resistant genotype (sem 05-500256) of brassica napus. colonization of both genotypes after dip-inoculation of the roots followed by quantitative polymerase chain reaction revealed similarities only in the initial stages of root penetration and colonization of the hypocotyl, while a substantial invasio ...200919522578
broadening the genetic basis of verticillium longisporum resistance in brassica napus by interspecific hybridization.abstract verticillium wilt caused by the vascular fungal pathogen verticillium longisporum is one of the most important pathogens of oilseed rape (brassica napus sp. oleifera) in northern europe. because production of this major oilseed crop is expanding rapidly and no approved fungicides are available for v. longisporum, long-term control of the disease can only be achieved with cultivars carrying effective quantitative resistance. however, very little resistance to v. longisporum is available ...200718943507
control of verticillium yellows in chinese cabbage by the dark septate endophytic fungus ltvb3.abstract three hundred forty-nine fungal endophytes were obtained from a total of 1,214 root segments of eggplant, melon, barley, and chinese cabbage grown as bait plants in a mixed soil made up of samples from different forest soils in alberta and british columbia, canada. three of the 349 isolates, when inoculated in axenically reared chinese cabbage seedlings grown in petri dishes, almost completely suppressed the effects of a postinoculated and virulent strain of verticillium longisporum. tw ...200418943758
identification of quantitative trait loci for resistance against verticillium longisporum in oilseed rape (brassica napus).verticillium longisporum is one of the major pathogens of oilseed rape (brassica napus; genome aacc, 2n = 38) in europe. current european cultivars possess only a low level of resistance against v. longisporum, meaning that heavy infection can cause major yield losses. the aim of this study was to identify quantitative trait loci (qtl) for resistance against v. longisporum as a starting point for marker-assisted breeding of resistant cultivars. resistance qtl were localized in a segregating oils ...200818943198
overexpression of a brassica nigra cdna gives enhanced resistance to leptosphaeria maculans in b. napus.using a polymerase chain reaction-based strategy, we have isolated a cdna sequence from brassica nigra, denoted lm1, which significantly improves blackleg resistance when expressed in transgenic oilseed rape, b. napus. lm1 was shown to map to locations on linkage groups 3 and 8 in the brassica b-genome previously linked to both cotyledon, adult leaf, and stem resistance. b. napus plants transformed with lm1 under the control of a constitutive promoter exhibited broad spectrum resistance to all l ...200312795374
screening of oxylipins for control of oilseed rape (brassica napus) fungal pathogens.oxylipins are products of oxygenase-catalyzed reactions of fatty acids. oxylipins have been found or implied to participate in a variety of different functions in or between organisms. in this report we investigated the potential of various naturally occurring oxylipins found in plants for their effects as fungicides on a number of fungal pathogens interfering with brassica cultivation. the fungi investigated were alternaria brassicae, leptosphaeria maculans, sclerotinia sclerotiorum and vertici ...200312657302
genetic and environmental control of the verticillium syndrome in arabidopsis thaliana.verticillium spp. are major pathogens of dicotyledonous plants such as cotton, tomato, olive or oilseed rape. verticillium symptoms are often ambiguous and influenced by development and environment. the aim of the present study was to define disease and resistance traits of the complex verticillium longisporum syndrome in arabidopsis thaliana (l.) heynh. a genetic approach was used to determine genetic, developmental and environmental factors controlling specific disease and resistance traits an ...201021044310
components of variance in transcriptomics based on electrophoretic separation of cdna fragments (cdna-aflp).the sources of variance and errors in transcriptomics based on the electrophoretic separation of amplified cdna fragments were investigated using cdna-amplified fragment length polymorphism (aflp). transcriptome profiles of the plant-pathogenic fungus verticillium longisporum were generated by a standard cdna-aflp protocol followed by electrophoretic separation of amplified dna fragments in flatbed polyacrylamide gels with fluorescence detection as well as by capillary electrophoresis (dna seque ...200919588459
interfamily transfer of tomato ve1 mediates verticillium resistance in arabidopsis.vascular wilts caused by soil-borne fungal species of the verticillium genus are devastating plant diseases. the most common species, v. dahliae and v. albo-atrum, have broad host ranges and are notoriously difficult to control. therefore, genetic resistance is the preferred method for disease control. only from tomato (solanum lycopersicum) a verticillium resistance locus has been cloned, comprising the ve1 gene that encodes a receptor-like protein-type cell surface receptor. due to lack of a s ...201121617027
salicylic acid and salicylic acid glucoside in xylem sap of brassica napus infected with verticillium longisporum.salicylic acid (sa) and its glucoside (sag) were detected in xylem sap of brassica napus by hplc-ms. concentrations of sa and sag in xylem sap from the root and hypocotyl of the plant, and in extracts of shoots above the hypocotyl, increased after infection with the vascular pathogen verticillium longisporum. both concentrations were correlated with disease severity assessed as the reduction in shoot length. furthermore, sag levels in shoot extracts were correlated with the amount of v. longispo ...200919449088
phylogenetic analysis of verticillium species based on nuclear and mitochondrial sequences.three different genes were sequenced from isolates of five plant-pathogenic verticillium species, verticillium albo-atrum, verticillium dahliae, verticillium longisporum, verticillium nigrescens, and verticillium tricorpus. the sequences covered parts of the mitochondrial cytochrome b gene ( cob), the mitochondrial small subunit rrna gene ( rns) and the nuclear its2 region. when the sequences were combined, the five species clustered in five monophyletic groups, with v. nigrescens distantly rela ...200415138663
the ascomycete verticillium longisporum is a hybrid and a plant pathogen with an expanded host range.hybridization plays a central role in plant evolution, but its overall importance in fungi is unknown. new plant pathogens are thought to arise by hybridization between formerly separated fungal species. evolution of hybrid plant pathogens from non-pathogenic ancestors in the fungal-like protist phytophthora has been demonstrated, but in fungi, the most important group of plant pathogens, there are few well-characterized examples of hybrids. we focused our attention on the hybrid and plant patho ...201121455321
a study on microbial diversity in different cultivars of brassica napus in relation to its wilt pathogen, verticillium longisporum.oilseed rape (brassica napus) is one of the major oilseed crops in the world but is vulnerable to attack by many pathogens and insect pests. in addition to the host plant genotype, micro-organisms present in the rhizosphere and within plant tissues affect the susceptibility to plant pathogens. while rapid progress has been achieved concerning the concept of plant resistance genes, information on the role of the microbial community in plant protection is less apparent. we have studied the endophy ...200312892892
molecular characterization of the host-adapted pathogen verticillium longisporum on the basis of a group-i intron found in the nuclear ssu-rrna gene.verticillium wilt of oilseed rape is caused by the host-adapted pathogen verticillium longisporum comb. nov. with one set of nuclear ssu-rrna gene primers, a pcr amplification product of ca. 2.5 kb was generated from all isolates of v. longisporum tested (36 from europe, japan, and usa), with the exception of two recombinant isolates. on the contrary, all the other phytopathogenic and nonphytopathogenic species of verticillium tested (18 species, 46 isolates), with the exception of one isolate o ...200111277099
protection of oilseed rape (brassica napus) toward fungal pathogens by strains of plant-associated bacillus amyloliquefaciens.in this report, four bacillus strains were tested for effects on plant fitness and disease protection of oilseed rape (brassica napus). the strains belonged to newly discovered plant-associated bacillus amyloliquefaciens and a recently proposed species, bacillus endophyticus. the fungal pathogens tested represented different infection strategies and included alternaria brassicae, botrytis cinerea, leptosphaeria maculans, and verticillium longisporum. the b. amyloliquefaciens strains showed no or ...200717186140
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