| tomato chlorotic dwarf viroid: an evolutionary link in the origin of pospiviroids. | over 40 isolates of potato spindle tuber viroid (pstvd) have been reported from potato, other solanum species and greenhouse tomato. these isolates have sequence similarities in the range 95-99%. a viroid which caused chlorotic leaves and severe dwarfing of plants in greenhouse tomato crops was detected. the viroid was found to hybridize readily with pstvd probes. it migrated faster than pstvd in return-polyacrylamide gel electrophoresis and was not amplified in rt-pcr by a primer pair based on ... | 1999 | 10580043 |
| an rt-pcr primer pair for the detection of pospiviroid and its application in surveying ornamental plants for viroids. | a primer pair for reverse transcription-polymerase chain reaction (rt-pcr), based on the conserved sequences of the members of genus pospiviroid was designed to yield a fragment of about 200 base pairs (bp). since pospiviroids infect a large number of plants species and a few members of the genus pospiviroid have been already detected in some ornamental plants, the primer pair was evaluated for its efficacy using ornamental plants. the method of return-polyacrylamide gel electrophoresis (r-page) ... | 2004 | 14738987 |
| a single polyprobe for detecting simultaneously eight pospiviroids infecting ornamentals and vegetables. | the spread of viroids belonging to the genus pospiviroid (family pospiviroidae), recorded recently in ornamentals and vegetables in several european countries, calls for fast, efficient and sensitive detection methods. based on bioinformatics analyses of sequence identity among all pospiviroids, a digoxigenin-labeled polyprobe (pospiprobe) was developed that, when tested by dot-blot and northern-blot hybridization, detected potato spindle tuber viroid, citrus exocortis viroid, columnea latent vi ... | 2012 | 22935607 |
| analysis of sequence polymorphism and population structure of tomato chlorotic dwarf viroid and potato spindle tuber viroid in viroid-infected tomato plants. | the sequence polymorphism and population structure of tomato chlorotic dwarf viroid (tcdvd) (isolate trust) and potato tuber spindle viroid (pstvd) (isolate fn) in tomato plants were investigated. of the 9 and 35 tcdvd clones sequenced from 2 different tcdvd-infected plants, 2 and 4 sequence variants were identified, respectively, leading to a total of 4 sequence variants of 360 nucleotides in length. variant i was identical to af162131, the first tcdvd sequence to be reported, and the rest exhi ... | 2012 | 22816033 |
| development of a rapid diagnostic assay for the detection of tomato chlorotic dwarf viroid based on isothermal reverse-transcription-recombinase polymerase amplification. | a molecular diagnostic assay utilizing reverse transcription-recombinase polymerase amplification (rt-rpa) at an isothermal constant temperature of 39°c and target-specific primers and probe were developed for the rapid, sensitive, and specific detection of tomato chlorotic dwarf viroid (tcdvd) in infected leaf and seed tissues. the performance of the amplifyrp(®) acceler8™ rt-rpa diagnostic assay, utilizing a lateral flow strip contained within an amplicon detection chamber, was evaluated and t ... | 2016 | 27427473 |
| λ-carrageenan suppresses tomato chlorotic dwarf viroid (tcdvd) replication and symptom expression in tomatoes. | the effect of carrageenans on tomato chlorotic dwarf viroid (tcdvd) replication and symptom expression was studied. three-week-old tomato plants were spray-treated with iota(ɩ)-, lambda(λ)-, and kappa(κ)-carrageenan at 1 g·l-1 and inoculated with tcdvd after 48 h. the λ-carrageenan significantly suppressed viroid symptom expression after eight weeks of inoculation, only 28% plants showed distinctive bunchy-top symptoms as compared to the 82% in the control group. viroid concentration was reduced ... | 2015 | 26006710 |