| whitefly transmission of sweet potato viruses. | three genera of plant viruses, begomovirus (geminiviridae), crinivirus (closteroviridae) and ipomovirus (potyviridae), contain members that infect sweet potato (ipomoea batatas) and are transmitted by whiteflies. the begomoviruses, sweet potato leaf curl virus (splcv) and ipomoea leaf curl virus (ilcv), and the ipomovirus sweet potato mild mottle virus are transmitted by bemisia tabaci, the sweet potato whitefly. the crinivirus, sweet potato chlorotic stunt virus (spcsv), is transmitted by b. ta ... | 2004 | 15036843 |
| molecular characterization of sweet potato leaf curl virus isolate from china (splcv-cn) and its phylogenetic relationship with other members of the geminiviridae. | a sweet potato-infecting sweet potato leaf curl virus (splcv) isolated in china was detected by polymerase chain reaction (pcr). pcr products amplified from dna-a were cloned and sequenced. the isolates of splcv from china(splcv-cn)has a genome organization similar to that of monopartite begomoviruses. the dna-a had two orfs (av1 and av2) in the virion sense and four orfs (ac1, ac2, ac3, and ac4) in the complementary sense, separated by an intergenic region (ir) containing a conserved stem-loop ... | 2007 | 17318425 |
| novel begomovirus species of recombinant nature in sweet potato (ipomoea batatas) and ipomoea indica: taxonomic and phylogenetic implications. | viral diseases occur wherever sweet potato (ipomoea batatas) is cultivated and because this crop is vegetatively propagated, accumulation and perpetuation of viruses can become a major constraint for production. up to 90% reductions in yield have been reported in association with viral infections. about 20 officially accepted or tentative virus species have been found in sweet potato and other ipomoea species. they include three species of begomoviruses (genus begomovirus, family geminiviridae) ... | 2009 | 19515824 |
| genetic diversity of sweet potato begomoviruses in the united states and identification of a natural recombinant between sweet potato leaf curl virus and sweet potato leaf curl georgia virus. | in the united states, two sweet potato begomoviruses, sweet potato leaf curl virus (splcv) and sweet potato leaf curl georgia virus (splcgv), were previously identified in louisiana. in recent years, at least seven additional sweet potato begomoviruses have been identified in other parts of the world. in an effort to determine the genetic diversity and distribution of sweet potato begomoviruses in the u.s., we focused our efforts on molecular characterization of field-collected begomovirus isola ... | 2011 | 21302123 |
| a novel monopartite begomovirus infecting sweet potato in brazil. | the complete genome sequences of two monopartite begomovirus isolates (genus begomovirus, family geminiviridae) present in a single sweet potato (ipomoea batatas) plant collected in são paulo, brazil, are presented. based on the current taxonomic criteria for the genus begomovirus, one of the isolates was shown to represent a novel species, tentatively named sweet potato leaf curl sao paulo virus (splcspv). the other isolate represented a new strain of sweet potato leaf curl virus, named sweet p ... | 2011 | 21594599 |
| molecular characterization of two sweepoviruses from china and evaluation of the infectivity of cloned splcv-js in nicotiana benthamiana. | sweepoviruses are important begomoviruses that infect ipomoea plants worldwide and cause sweet potato yield losses and cultivar decline. two sweepoviruses, sweet potato leaf curl virus-jiangsu (splcv-js) and sweet potato leaf curl china virus-zhejiang (splccnv-zj), were cloned from diseased sweet potato plants collected in the jiangsu and zhejiang provinces of china. sequence characterization and phylogenetic analysis demonstrated that both are typical monopartite begomoviruses and have close re ... | 2011 | 22179901 |
| molecular characterization of sweet potato leaf curl virus (splcv) isolates from korea: phylogenetic relationship and recombination analysis. | the complete dna genome of sweet potato leaf curl virus (splcv) from samples obtained from eight regions was amplified by pcr and characterized in this study. the dna genome of one group (splcv korea group 1) consisted of 2828 nucleotides and that of the second group (splcv korea group 2) consisted of 2829 nucleotides. sequence comparisons showed that the genome sequences of splcv korea isolates were closely related to those of splcv brazil isolates (fj969834, fj969835, and fj969836), splcv japa ... | 2011 | 22149498 |
| the current incidence of viral disease in korean sweet potatoes and development of multiplex rt-pcr assays for simultaneous detection of eight sweet potato viruses. | sweet potato is grown extensively from tropical to temperate regions and is an important food crop worldwide. in this study, we established detection methods for 17 major sweet potato viruses using single and multiplex rt-pcr assays. to investigate the current incidence of viral diseases, we collected 154 samples of various sweet potato cultivars showing virus-like symptoms from 40 fields in 10 korean regions, and analyzed them by rt-pcr using specific primers for each of the 17 viruses. of the ... | 2014 | 25506306 |
| complete genome sequence of a novel monopartite begomovirus infecting sweet potato in china. | the complete genome sequence of a new monopartite begomovirus isolate sc-1 was obtained from sweet potato samples in sichuan province, china. the viral genome consists of 2,764 nucleotides (nt) and encodes two open reading frames (orfs) called av1 and av2 genes in the viral-sense strand and four orfs (ac1-ac4) in the complementary-sense strand. sequence comparisons revealed that it shared the highest level of nt sequence identity (81.2 %) with sweet potato leaf curl georgia virus (af326775). phy ... | 2013 | 24057883 |
| infectivity, effects on helper viruses and whitefly transmission of the deltasatellites associated with sweepoviruses (genus begomovirus, family geminiviridae). | begomoviruses (family geminiviridae) are whitefly-transmitted viruses with single-stranded dna genomes that are frequently associated with dna satellites. these satellites include non-coding satellites, for which the name deltasatellites has been proposed. although the first deltasatellite was identified in the late 1990s, little is known about the effects they have on infections of their helper begomoviruses. recently a group of deltasatellites were identified associated with sweepoviruses, a g ... | 2016 | 27453359 |
| agroinfection of sweet potato by vacuum infiltration of an infectious sweepovirus. | sweepovirus is an important monopartite begomovirus that infects plants of the genus ipomoea worldwide. development of artificial infection methods for sweepovirus using agroinoculation is a highly efficient means of studying infectivity in sweet potato. unlike other begomoviruses, it has proven difficult to infect sweet potato plants with sweepoviruses using infectious clones. a novel sweepovirus, called sweet potato leaf curl virus-jiangsu (splcv-js), was recently identified in china. in addit ... | 2014 | 24903591 |
| natural association of two different betasatellites with sweet potato leaf curl virus in wild morning glory (ipomoea purpurea) in india. | wild morning glory (ipomoea purpurea) was observed to be affected by leaf curl and yellow vein diseases during summer-rainy season of 2009 in new delhi, india. the virus was experimentally transmitted through whitefly, bemisia tabaci to i. purpurea that reproduced the two distinct symptoms. sequence analysis of multiple full-length clones obtained through rolling circle amplification from the leaf curl and yellow vein samples showed 91.8-95.3% sequence identity with sweet potato leaf curl virus ... | 2013 | 23529301 |
| molecular characterization and an infectious clone construction of sweet potato leaf curl virus (splcv) isolated from korea. | sweet potato leaf curl disease (splcd) was primarily identified in sweet potato fields in korea in 2003, and the complete genomic sequence of sweet potato leaf curl virus (splcv) has been cloned. the genome of the korean splcv isolate (splcv-kr) comprises 2,828 nucleotides with six open reading frames in dna-a, similar to a monopartite begomovirus. additionally, neither the genome b genomic component nor the dna beta sequence was detected. the results of phylogenetic analysis using the maximum p ... | 2012 | 23043598 |
| molecular characterization of sweet potato leaf curl virus isolate from china (splcv-cn) and its phylogenetic relationship with other members of the geminiviridae. | | 2007 | 27517684 |