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drastic post-lgm expansion and lack of historical genetic structure of a subtropical fig-pollinating wasp (ceratosolen sp. 1) of ficus septica in taiwan.the climatic oscillations of the last glacial period have had profound influences on the demography and levels of genetic diversity of extant species. molecular evidence of glacial effects on temperate species has been well documented, whereas little is known regarding that on subtropical species. here we present analyses based on partial sequences of the mitochondrial cytochrome c oxidase subunit i (coi) gene (1052 bp) and genotypes at 15 microsatellite loci to investigate the historical demogr ...200819120988
antimalarial phenanthroindolizine alkaloids from ficus septica.during the screening of antimalarial substances, meoh extract from the twigs of ficus septica was shown to have potent antimalarial activity. bioassay-guided fractionation of a methanol extract of the twigs of f. septica led to the isolation of a new seco-phenanthroindolizine alkaloid and three known phenanthroindolizine alkaloids. their structures were elucidated on the basis of nmr analysis. all isolated compounds were tested against plasmodium falciparum. compounds 2-4 displayed antimalarial ...201627373653
temperature controls on the basal emission rate of isoprene in a tropical tree ficus septica: exploring molecular regulatory mechanisms.isoprene emission from plants is very sensitive to environmental temperature both at short-term and long-term scales. our previous study demonstrated suppression of isoprene emission by cold temperatures in a high emitting tropical tree ficus septica and revealed a strong correlation of emission to isoprene synthase (isps) protein levels. when challenged with decreasing daily temperatures from 30 to 12 °c, f. septica completely stopped isoprene emission at 12 °c, only to recover on the second da ...201627425779
gene expression analysis of disabled and re-induced isoprene emission by the tropical tree ficus septica before and after cold ambient temperature exposure.isoprene is the most abundant type of nonmethane, biogenic volatile organic compound in the atmosphere, and it is produced mainly by terrestrial plants. the tropical tree species ficus septica burm. f. (rosales: moraceae) has been shown to cease isoprene emissions when exposed to temperatures of 12 °c or lower and to re-induce isoprene synthesis upon subsequent exposure to temperatures of 30 °c or higher for 24 h. to elucidate the regulation of genes underlying the disabling and then induction o ...201627126228
cytotoxic effect and constituent profile of alkaloid fractions from ethanolic extract of ficus septica burm. f. leaves on t47d breast cancer cells.the study aimed to investigate the profile of alkaloids in two ethyl acetate soluble fractions, namely fractions a and b from an ethanolic extract of ficus septica leaves and cytotoxic effect on t47d breast cancer cells. preparation of both fractions involved maceration of leaves with 70% (v/v) ethanol, filtration with al2o3, precipitation with 0.1 n hcl, mayer reagent, and 0.1 n naoh, and also partition with ethyl acetate. qualitative thin layer chromatography (tlc) was conducted to determine t ...201526514534
molecular cloning and biochemical characterization of isoprene synthases from the tropical trees ficus virgata, ficus septica, and casuarina equisetifolia.three isoprene synthase (isps) cdna clones have been isolated from tropical trees (ficus septica, f. virgata, and casuarina equisetifolia), and their enzyme properties have been compared with those of populus alba isps. phylogenetic analysis of the deduced amino acid sequences with known monoterpene synthase resolved isps from f. septica and f. virgata and other ispss in a clade together with tps-b clade i, whereas isps from c. equisetifolia was within another clade, sister to tps-b clade ii. th ...201526081976
daily rhythm of mutualistic pollinator activity and scent emission in ficus septica: ecological differentiation between co-occurring pollinators and potential consequences for chemical communication and facilitation of host speciation.the mutualistic interaction between ficus and their pollinating agaonid wasps constitutes an extreme example of plant-insect co-diversification. most ficus species are locally associated with a single specific agaonid wasp species. specificity is ensured by each fig species emitting a distinctive attractive scent. however, cases of widespread coexistence of two agaonid wasp species on the same ficus species are documented. here we document the coexistence of two agaonid wasp species in ficus sep ...201425105796
temperature threshold of isoprene emission from tropical trees, ficus virgata and ficus septica.this paper describes the existence of temperature threshold in isoprene emission from tropical trees for the first time. isoprene emission capacity of ficus virgata leaves kept outdoors were measured over a period of 20 d in the wintertime. when the plants experienced the low temperature below 12 °c in the daytime, isoprene emission was completely suppressed, and re-activated by elevated temperature above the threshold of 12 °c. photosynthesis is also decreased similarly, but too much smaller ex ...201424080006
combinational effects of hexane insoluble fraction of ficus septica burm. f. and doxorubicin chemotherapy on t47d breast cancer cells.to evaluate the effects of n-hexane insoluble fraction (hif) of ficus septica leaves in combination with doxorubicin on cytotoxicity, cell cycle and apoptosis induction of breast cancer t47d cell lines.201323620854
immunomodulatory effects of hexane insoluble fraction of ficus septica burm. f. in doxorubicin-treated rats.the use of chemotherapeutics induces cardiotoxicity and affects immune functions, therefore development of combinatorial agents against cardiotoxicity and immunosuppression needs to be explored. previous studies of the hexane insoluble fraction (hif) of an ethanolic extract of ficus septica leaves showed anticancer effects singly and in combination with doxorubicin on t47d breast cancer cells. in this present study, it was evaluated for its immunomodulatory activities in doxorubicin-treated rats ...201223317257
synergistic effects of ethyl acetate fraction of ficus septica burm. f. and doxorubicin chemotherapy on t47d human breast cancer cell line.previously, ethanolic extract of ficus septica burm. f. (moraceae) leaves and its ethyl acetate soluble fraction (easf) exhibited potent cytotoxic effects on t47d breast cancer cells. in the present study, we further investigated the effects of easf of ethanolic extract of f. septica leaves in combination with doxorubicin on t47d breast cancer cell line in cytotoxicity, cell cycle arrest and apoptosis induction.201223073201
aminocaprophenone- and pyrrolidine-type alkaloids from the leaves of ficus septica.two new aminocaprophenone alkaloids, ficuseptamines a (1) and b (2), and a new pyrrolidine alkaloid, ficuseptamine c (3), together with 12 known alkaloids and a known acetophenone derivative were isolated from a methanolic extract of the leaves of ficus septica. the structures of 1-3 were determined on the basis of their spectroscopic data. the compounds obtained were evaluated for cytotoxicity against two cancer cell lines.200919938815
cytotoxic phenanthroindolizidine alkaloids from the roots of ficus septica.activity-monitored fractionation of the chcl3 solubles of the meoh extract of the roots of ficus septica using cytotoxicity assay led to the isolation of twenty phenanthroindolizidine alkaloids, including ten new ficuseptines e-n and ten known compounds. the cytotoxic effects of some of the isolates were tested on gastric adenocarcinoma (nugc) and nasopharyngeal carcinoma (hone-1) cell lines and the results demonstrated that, with the exception of dehydrotylophorine, all the tested compounds dis ...200919296431
phenanthroindolizidine alkaloids from the stems of ficus septica.in addition to six known phenanthroindolizidine alkaloids, eight new alkaloids, namely, ficuseptines b-d (1-3), 10r,13ar-tylophorine n-oxide (4), 10r,13ar-tylocrebrine n-oxide (5), 10s,13ar-tylocrebrine n-oxide (6), 10s,13ar-isotylocrebrine n-oxide (7), and 10s,13as-isotylocrebrine n-oxide (8), were isolated from a methanol extract of the stems of ficus septica. the structures of the new compounds were elucidated by means of spectroscopic data interpretation. cytotoxicity of some of these alkalo ...200516038551
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