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a new fast real-time pcr method for the identification of three sibling apodemus species (a. sylvaticus, a. flavicollis, and a. alpicola) in italy.the identification of field mice apodemus flavicollis, apodemus sylvaticus, and apodemus alpicola represents a challenge for field scientists due to their highly overlapping morphological traits and habitats. here, we propose a new fast real-time pcr method to discriminate the three species by species-specific taqman assays. primers and probes were designed based on the alignment of 54 cyt-b partial sequences from 25 different european countries retrieved from genbank. taqman assays were then te ...201829876059
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