Publications

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aroapyrgus costaricensis, hydrobiid snail host of paragonimiasis in costa rica.a small freshwater hydrobiid snail found in narrow shallow steams at various altitudes in costa rica is the intermediate host of paragonimus mexicanus. based on the morphology of its shell and soft parts the snail is assigned to the species aroapyrgus costaricensis (mörch). it is easily maintained in the laboratory; the female is ovoviviparous, carries up to 40 embryos in its brood pouch, and young-to-young cycle is about 10 weeks. natural infection rates with rediae and cercariae of paragonimus ...19751127567
paragonimiasis in ecuador: prevalence and geographical distribution of parasitisation of second intermediate hosts with paragonimus mexicanus in esmeraldas province.to determine whether the human cases of pulmonary paragonimiasis detected in the province of esmeraldas were autochthonous a total of 1,457 freshwater crabs, species hypolobocera aequatorialis, were collected from 45 different streams in 4 cantons to determine the rate of parasitisation with metacercariae of paragonimus mexicanus. some 28 (62.2%) streams were found to harbour parasitised crabs, with 76.5% of the streams in canton quininde infected, followed by those in muisne, 72.7%, esmeraldas, ...19921293730
intermediate hosts of paragonimus in the eastern amazonic region of ecuador.a study was done to determine the intermediate hosts of paragonimus mexicanus (p. peruvianus, p. ecuadoriensis) in the northeastern amazonic region of ecuador. the first intermediate host belongs to the molluscan family hydrobiidae, similar to the findings reported in other parts of latin america. one of 2350 specimens of the hydrobiid snail aroapyrgus colombiensis examined was found to be naturally infected with rediae containing microcercous cercariae of paragonimus, this being the first natur ...19911801137
experimental infection of paragonimus mexicanus in oncomelania nosophora. 19873598814
paragonimus mexicanus (trematoda) in costa rica in two new intermediate hosts: ptychophallus richmondi and ptychophallus tumimanus (crustacea: decapoda). 19863806327
[natural infection of ptychophallus tristani (crustacea: decapoda) by metacercariae of paragonimus mexicanus (trematoda) in tabarcia de mora, costa rica]. 19854035204
paragonimus mexicanus: an antigenic analysis. 19826821233
paragonimus mexicanus pericarditis: report of two cases and review of the literature.the cases of two costa rican children with pericarditis due to paragonimus mexicanus are reported. clinical, epidemiological and laboratory tests are consistent with the disease. treatment with praziquantel and bitheonol was associated with clinical cure. a review of the literature and a suggested table of diagnostic criteria are included.19957563258
treatment of human pulmonary paragonimiasis with triclabendazole: clinical tolerance and drug efficacy.an open clinical trial to determine the efficacy and tolerability of postprandial doses of triclabendazole against paragonimus mexicanus in 62 patients with pulmonary paragonimiasis from the ecuadorian amazon region was performed. praziquantel was used as therapeutic control. patients were allocated at random to the following 4 therapeutic regimens: triclabendazole, 5 mg/kg once daily for 3 d (16 patients), 10 mg/kg twice on one day (15 patients), and 10 mg/kg in a single dose (16 patients), and ...19989861383
a molecular phylogeographic study based on dna sequences from individual metacercariae of paragonimus mexicanus from guatemala and ecuador.a molecular phylogeographic study of paragonimus mexicanus collected from guatemala and ecuador was performed. genomic dna was extracted from individual metacercariae, and two gene regions (partial mitochondrial cytochrome c oxidase subunit 1 (co1) and the second internal transcribed spacer of the nuclear ribosomal gene repeat (its2)) were amplified by the polymerase chain reaction (pcr). sequences segregated in a phylogenetic tree according to their geographic origins. its2 sequences from ecuad ...200312590662
lung-migrating digenean parasites: in vitro influence on nitric oxide production from normal rat pulmonary macrophages.nitric oxide (no) is one of the most versatile players in the immune system. most parasites induce inflammation in the host associated with no production. here, we compare the in vitro effect of schistosoma bovis somatic (sbs) and excretory-secretory (sbes) antigens, and excretory-secretory paragonimus mexicanus adult worm (pmes) molecules on rat alveolar macrophages no production measured by the griess method and by rt-pcr. additionally, we address the divergence of the no stimulatory/inhibitor ...200515713448
pulmonary paragonimiasis diagnosed by fine-needle aspiration biopsy.we report a case of paragonimiasis involving a 12-year-old latin american boy. the diagnosis was made by fine-needle aspiration biopsy of a pulmonary nodule. identification of the species by morphometric analysis of the eggs indicated that the infection was caused by paragonimus mexicanus.200818385444
enzyme-linked immunosorbent assay for diagnosis of amphimerus spp. liver fluke infection in humans.amphimerus spp. is a liver fluke that infects humans and domestic animals. it is highly prevalent in some ecuadorian communities. currently, diagnosis is based on the microscopic observation of eggs in faeces, but this has variable sensitivity. more sensitive methods are needed for diagnostic testing.201728443982
detection of multiple species of human paragonimus from mexico using morphological data and molecular barcodes.paragonimus mexicanus is the causal agent of human paragonimiasis in several countries of the americas. it is considered to be the only species of the genus present in mexico, where it is responsible for human infection. through the investigation of p. mexicanus specimens from several places throughout mexico, we provide morphological, molecular and geographical evidence that strongly suggests the presence of at least three species from this genus in mexico. these results raise questions regardi ...201323530893
detection of paragonimus mexicanus (trematoda) metacercariae in crabs from oaxaca, mexico.metacercariae of paragonimus mexicanus were collected in crabs tehuana guerreroensis (rathbun, 1933) in the municipality of putla, oaxaca, mexico. metacercariae were found in 20.8% of the crabs collected, with an average of 1.9 metacercarie per crab. stained metacercariae showed the specific characteristics of p. mexicanus by morphology and sequencing a fragment of the 28s ribosomal gene obtained by pcr. these findings reveal that t. guerreroensis is an intermediate host for p. mexicanus; this n ...201424836918
current status of paragonimus and paragonimiasis in ecuador.a review of national and international publications on paragonimiasis in ecuador, epidemiological records from the ministry of public health and unpublished research data was conducted to summarise the current status of the parasite/disease. the purpose of the review is to educate physicians, policy-makers and health providers on the status of the disease and to stimulate scientific investigators to conduct further research. paragonimiasis was first diagnosed in ecuador 94 years ago and it is en ...201425338151
current status of paragonimus and paragonimiasis in ecuador.a review of national and international publications on paragonimiasis in ecuador, epidemiological records from the ministry of public health and unpublished research data was conducted to summarise the current status of the parasite/disease. the purpose of the review is to educate physicians, policy-makers and health providers on the status of the disease and to stimulate scientific investigators to conduct further research. paragonimiasis was first diagnosed in ecuador 94 years ago and it is en ...201425410987
severe pleuropulmonary paragonimiasis caused by paragonimus mexicanus treated as tuberculosis in ecuador.a 30-year-old male, from a subtropical region of ecuador, was hospitalized with a 5-year history of persistent cough with rusty brown sputum, chest pain, and progressive dyspnea. the patient underwent thoracic surgery 3 years ago for pleural effusion and subsequently received a 9-month regimen treatment of tuberculosis. however, there was no clinical resolution and symptoms became progressively worse. a chest radiograph and computerized tomography scan showed several small nodules in both lungs. ...201727879464
morphological and molecular characterization of the metacercaria of paragonimus caliensis, as a separate species from p. mexicanus in costa rica.the trematode paragonimus mexicanus is the etiological agent of paragonimiasis, a food-borne zoonotic disease in latin america. this species, as well as paragonimus caliensis, have been reported from costa rica, but it is not known if the two are synonymous. two types of paragonimus metacercariae from freshwater pseudothelphusid crabs from several localities in costa rica were recognized by light microscopy. morphologically, these corresponded to descriptions of p. mexicanus and p. caliensis. me ...201728027969
cerebral hemorrhagic lesions produced by paragonimus mexicanus. report of three cases in costa rica.three cases of cerebral lesions due to paragonimus mexicanus in costa rica are reported, two of which were fatal. at autopsy a hemorrhagic, well circumscribed lesion was found in the hemispheres which microscopically consisted of a recent hemorrhage surrounded by a halo of eosinophils, with giant cell granulomas and charcot-leyden crystals. eggs of p. mexicanus outside the brain were demonstrated in both fatal cases--in an eosinophilic pericarditis in one and in the other in multiple lesions of ...19827081546
aroapyrgus alleei morrison, 1946 (gastropoda: hydrobiidae) first intermediate host of paragonimus mexicanus in colima, mexico.the lung fluke paragonimus mexicanus infects opossums in the state of colima, on the pacific coast of mexico. some of the hydrobiid snails occurring in the same area, and identified as aroapyrgus alleei morrison, 1946, were found naturally infected with rediae and cercariae of p. mexicanus. the morphology of a. alleei is here described and compared to that of other species of aroapyrgus, which are actual or potential hosts of lung flukes in other enzootic or endemic areas in central and south am ...19836827440
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