| analysis of antibody levels before and after praziquantel treatment in human paragonimiasis heterotremus. | enzyme immunoassays (elisa) and western blot analysis were used to determine igg antibody levels in patients infected with paragonimus heterotremus from thailand before and after treatment with praziquantel. an igg antibody elisa showed that a substantial reduction of antibody levels occurred after one year of treatment. sodium dodecyl sulphate-polyacrylamide gel electrophoresis (sds-page) and western blot analysis showed that p. heterotremus adult extract is highly complex, consisting of more t ... | 1992 | 1418188 |
| electrophoretic analysis of a natural population of the thai paragonimus heterotremus and its genetic relationship to the three japanese species p. miyazaki, p. ohirai and p. westermani. | a total of 18 enzymes (encoded by 20 loci) from paragonimus heterotremus collected at a local area in saraburi, thailand, were electrophoretically compared with those from the three japanese species p. ohirai, p. miyazaki and p. westermani using horizontal starch-gel electrophoresis. relative genetic distances between these species were quantified. the thai p. heterotremus was found to be most closely related to p. miyazaki and rather distantly related to p. westermani. on the other hand, among ... | 1992 | 1438131 |
| excretory-secretory antigenic components of paragonimus heterotremus recognized by infected human sera. | antigenic components of paragonimus heterotremus metabolic products were revealed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (sds-page) and immunoblot analysis of sera from patients with p. heterotremus infection, from patients with other illnesses, and from healthy adults. by sds-page, it was found that the metabolic products comprised more than eight major polypeptides. immunoblot analysis revealed 11 components which were strongly recognized by paragonimiasis antisera. these ... | 1992 | 1500515 |
| antigenic components of paragonimus heterotremus recognized by infected human serum. | the antigenic components of a paragonimus heterotremus saline extract were revealed by sds-page and western blot analysis using sera from 32 patients with p. heterotremus infection, 60 with other helminthiasis, and 15 normal human sera. it was found that the worm extract was comprised of more than 13 polypeptides, among which 5 components were strongly recognized by paragonimiasis sera. these components had approximately showed molecular weight of less than 12.3, 12.3, 18.5, 31,.5 and 38 kd. onl ... | 1991 | 1707511 |
| studies on immunodiagnosis of human paragonimiasis and specific antigen of paragonimus heterotremus. | adult paragonimus heterotremus were recovered from the lungs and pleural cavity of cats orally infected with metacercariae. the worms were ground and extracted with distilled water. the soluble crude antigen (ca) contained about 40% proteins which could be fractionated by gel filtration on sephadex g-200 into three profiles namely the f1, f2 and f3. the ca and its sephadex profiles were used in an indirect enzyme-linked immunosorbent assay (elisa) for detecting antibodies to p. heterotremus in t ... | 1991 | 1917281 |
| scanning electron microscopy of cuticular surface of the adults paragonimus heterotremus in phitsanulok, thailand. | scanning electron microscope observation of surface structure was made on adult paragonimus heterotremus (phitsanulok, thailand) and the surface topography of the anterior end, suckers, body cuticle and papillae was described. the numerous thorn-like tegumentary spines were distributed all over the surface. the spines were well-developed and branched. the papillae were divided into two types: dome-shaped and ciliated. two pairs of papillae were seen around the lip of the oral sucker, while those ... | 1991 | 1948269 |
| comparison of adult somatic and excretory-secretory antigens in enzyme-linked immunosorbent assay for serodiagnosis of human infection with paragonimus heterotremus. | adult somatic antigen extract of paragonimus heterotremus was compared with excretory-secretory (es) antigen in an enzyme-linked immunosorbent assay (elisa) for serodiagnosis of human paragonimiasis. the absorbence values in elisa using the adult somatic antigen were not significantly different from the values obtained using es antigens (p greater than 0.05). the sensitivity of the elisa using either antigen was 100%, but the specificity was 96% with somatic and 98% with es antigens due to a cro ... | 1990 | 2096519 |
| serodiagnosis of human paragonimiasis caused by paragonimus heterotremus. | enzyme-linked immunosorbent assay (elisa) and indirect hemagglutination tests (iha) were evaluated for serodiagnosis of human paragonimiasis caused by paragonimus heterotremus using homologous adult worm extract as antigen. igg-elisa was the most sensitive, being positive in all paragonimiasis sera tested while iha and iga-elisa gave 88% and 59% positive rates respectively. cross reactivity in igg-elisa was detected with fascioliasis sera, producing overall assay specificity of 97%. it is sugges ... | 1990 | 2402668 |
| surface ultrastructure of newly excysted metacercariae of paragonimus heterotremus. | the newly excysted metacercariae of p. heterotremus were examined by scanning electron microscopy (sem). large dome-shaped papillae, small ones with a pit, and small ones with a smooth surface were observed around the suckers. the number of the small dome-shaped papillae with a pit was about 30 around the oral sucker and that of the small ones with a smooth surface varied from 9 to 13 around the ventral sucker. the present report is the first record of excysted metacercariae of p. heterotremus e ... | 1990 | 2402669 |
| susceptibility of tricula aperta (beta race) to paragonimus heterotremus. | | 1988 | 3227413 |
| distribution of paragonimus heterotremus metacercariae in fresh water crab, tiwaripotamon beusekomae-bott 1970. | | 1988 | 3227414 |
| paragonimus heterotremus and other paragonimus spp. in thailand: pathogenesis, clinic and treatment. | six species of paragonimus have been reported in thailand: p. siamensis in cat, bandicoot and rat; p. bangkokensis in mongoose; p. harinasutai in cat and dog (experiment); p. macrochis in bandicoot and rat; p. westermani in tiger and p. heterotremus in cat, dog and man. it is interesting to note that in 1965 two immature p. heterotremus worms were recovered for the first time in man, namely in subcutaneous swellings in a boy; in 1981 nine mature p. heterotremus worms were expectorated after praz ... | 1984 | 6542391 |
| pulmonary paragonimiasis with expectoration of worms: a case report. | a 40-year old thai farmer with pulmonary paragonimiasis was treated with praziquantel 25 mg/kg body weight, three times after meals for one day. on follow-up treatment during hospitalization he coughed up 7 adult lung flukes. the worms were identified as paragonimus heterotremus. this is probably the first case report to demonstrate adult mature paragonimus coughed up from man. | 1981 | 7256348 |
| partially purified antigens of paragonimus heterotremus for serodiagnosis of human paragonimiasis. | the preparative crude extract of paragonimus heterotremus was fractionated by isoelectric focusing. fractions at ph 5 which contained a specific antigen with a relative molecular weight of 31.5 kda were pooled and used in an indirect enzyme-linked immunosorbent assay (elisa) and immunoblot analysis for diagnosis of human paragonimiasis. the sensitivity and specificity of elisa were found to be 100% and 99% respectively. the band of 31.5 kda antigenic component was found to give consistent reacti ... | 1994 | 7825009 |
| [discovery of the first intermediate host of paragonimus heterotremus]. | second generation rediae and cercariae of paragonimus heterotremus were discovered from tricula sp. collected from paragonimus heterotremus endemic area, napo county, guangxi zhuan autonomous region. the infection rate of the snails was 0.11% (4/3 530). the experimental infection of tricula sp. collected from wuming non-endemic area was done with p. heterotremus miracidia. the second generation rediae and cercariae of p. heterotemus developed later in the experimentally infected snails were simi ... | 1994 | 8044902 |
| [scanning electron microscopic observation on the encysted and excysted metacercariae of paragonimus heterotremus]. | the surface ultrastructure of metacercariae of paragonimus heterotremus was studied by scanning electron microscope. encysted metacercaria was egg-shaped with a button-like structure on its end. the oral sucker of the newly excysted metacercaria was larger than the ventral sucker. single-pointed spines were covered densely on all of the tegumental surface. there were two and three rings sensory papillae on the oral and ventral suckers, respectively. six papillae of the most inner ring were distr ... | 1993 | 8174217 |
| monoclonal antibodies to paragonimus heterotremus and their potential for diagnosis of paragonimiasis. | monoclonal antibodies (mabs) specific to the lung fluke (paragonimus heterotremus) were produced against the soluble metabolic products (excretory-secretory antigen). three hybrids secreting mabs specific for p. heterotremus antigens were identified by an indirect enzyme-linked immunosorbent assay (elisa) against a panel of homologous and 24 heterologous parasite antigens and mycobacterium tuberculosis. of the three specific clones, clone 10f2, which was igg1 producing and which gave immune comp ... | 1997 | 9158050 |
| parasites elicited cross-reacting antibodies to opisthorchis viverrini. | two batches of crude antigens extracted from adult opisthorchis viverrini worms were compared. one was derived from adult worms harvested from the livers of laboratory infected hamsters and another was obtained from worms sedimented from the faeces of opisthorchiasis patients following treatment with praziquantel. sds-page and coomassie brilliant blue staining revealed that the two preparations had similar protein components of which the predominant ones were the 17-18 kda doublet. the antigens ... | 1997 | 9346276 |
| detection of paragonimus heterotremus in experimentally infected cat feces by antigen capture-elisa and by dna hybridization. | an antigen capture enzyme-linked immunosorbent assay (antigen capture-elisa) and dna hybridization technique were developed and evaluated for their application in the detection of paragonimus heterotremus infection in experimentally infected cats. an igg fraction prepared from serum of a rabbit immunized with p. heterotremus excretory-secretory (es) products was used as the capture antibody. an igg1 monoclonal antibody specific to the 22- and 31.5-kda es products of p. heterotremus was used as t ... | 1997 | 9406782 |
| human lung fluke paragonimus heterotremus: differential diagnosis between paragonimus heterotremus and paragonimus westermani infections by eitb. | the specificity of three major polypeptides (35, 33 and 32.5 kd) from paragonimus heterotremus antigens prepared from ether-extracted adult worms was tested against sera from heterologous infections as well as against p. westermani-infected sera. only the 35 kd polypeptide was not present, its antigenic determinant being bound to the antibodies from all p. westermani-infected cases. its cross-reactivity against various sera from heterologous helminthiases and other lung infections showed that it ... | 1998 | 9484969 |
| indirect haemagglutination test using monoclonal antibody-affinity purified antigens for diagnosis of human paragonimiasis due to paragonimus heterotremus. | an indirect haemagglutination test (iha) using antigens purified by monoclonal antibody-affinity chromatography was developed for the diagnosis of human paragonimiasis caused by paragonimus heterotremus. sera from patients with paragonimiasis (n = 30) were evaluated, along with sera from other parasitic infections (n = 92), pulmonary tuberculosis (n = 18) and healthy controls (n = 30). the sensitivity, specificity as well as positive and negative predictive value of the iha, calculated at the pr ... | 1998 | 9484970 |
| a dot-elisa test using monoclonal antibody-purified antigens for the diagnosis of paragonimiasis caused by paragonimus heterotremus. | a dot enzyme-linked immunosorbent assay (dot-elisa) using antigens purified by monoclonal antibody-affinity chromatography was developed for detecting antibodies to paragonimus heterotremus in four groups of subjects. they consisted of 30 patients with p. heterotremus infection, 93 patients with other parasitic infections, 18 patients with pulmonary tuberculosis and 30 normal, healthy controls. sensitivity, specificity, as well as positive and negative predictive values of the test were 100, 97, ... | 1997 | 9561619 |
| recent advances in diagnosis of paragonimiasis. | paragonimiasis in endemic areas can be diagnosed by clinical symptoms. however, the diagnosis should always be confirmed by microscopic examination of the sputum or stool in order to find paragonimus eggs. within recent years marked advances in diagnosis of paragonimiasis have been made. two new approaches comprising a genetic probe and immunological tests have been developed with claims to be as good or better than microscopic examinations. this report reviews these two areas, especially in par ... | 1997 | 9656364 |
| use of delipidized antigens of taenia solium metacestodes in igg-elisa for detection of neurocysticercosis. | the development of igg-elisa for detecting neurocysticercosis is aimed at the routine laboratory, and requires a particular antigen preparation, an acceptable number of serum samples to be tested (both homologous and heterologous) and patients with a diversity of helminthic infections to rule out cross-reactions. this study characterizes igg-antibodies from cases of neurocysticercosis by assaying the sera against ether-delipidized antigens (5 microg/ml) prepared from metacestodes of taenia soliu ... | 1998 | 10437960 |
| epidemiology, symptoms and treatment of paragonimiasis in sin ho district, lai chau province, vietnam. | sin ho is a district of the northern mountainous province of lai chau, vietnam, where the people have the habit of eating undercooked crabs. a study on paragoniamiasis carried out from 1994 to 1995 with 1,642 persons in this endemic area, showed that the rate of eating raw-crab was 72.5%. crab examination (ranguna kimboiensis) showed an infection rate of paragonimus metacercaria of 98.1%. with 624 stool samples examined by kato technique, the infection rate of paragonimus in humans was 6.4%. wit ... | 2000 | 11414455 |
| comparative studies on protein, isoenzyme and dna restriction endonuclease profiles of thai paragonimus metacercariae. | general proteins and 14 enzymes from metacercariae of paragonimus heterotremus, p. siamensis and p. westermani were determined by vertical polyacrylamide gel electrophoresis. the isoenzyme profiles showed considerable interspecific polymorphism for general protein (pt), malate dehydrogenase (mdh), malic enzyme (me) and tetrazolium oxidase (to) while those of glucose phosphate isomerase (gpi) and phosphoglucomutase (pgm) showed similarity. the pt-6 and to-i loci can be used as identification mark ... | 2000 | 11414457 |
| angiostrongyliasis: analysis of antigens of angiostrongylus costaricensis adult worms versus igg from infected patients with angiostrongylus cantonensis. | the possibility of cross-reactivity was previously investigated by indirect elisa with sera from angiostrongylus cantonensis infections, normal controls and a. costaricensis antigen. 5 microg/ml of crude antigen from both sexes of each species reacted with diluted serum samples (1:800) of each of 20 cases of angiostrongyliasis and normal controls, and further with anti-human igg conjugate at 1:1,000. the mean absorbance values were evaluated as follows; normal controls showed a value of 0.033 us ... | 2000 | 11414459 |
| in vitro excystation of paragonimus heterotremus metacercariae. | in vitro excystation studies were carried out on the metacercariae cysts of paragonimus heterotremus obtained from naturally infected crabs potamon spp. the effects of elastase, trypsin, trypsin-dog bile, trypsin-bile salt, and dithiothreitol (dtt) were examined. the trypsin-dog bile medium stimulated maximum excystation. of the media that contained 1 mm dtt, the optimum conditions for the excystation were shown to be ph 9, temperature of 39-40 c, and osmolarity of 250-350 mosm. the dtt accelera ... | 2001 | 11695393 |
| the present situation of paragonimiasis in endemic area in phitsanulok province. | a cross-sectional survey and evaluation of paragonimiasis situation from endemic area in phitsanulok province was studied. studies on the species and prevalence of parasites which infected people in noen maprang, phitsanulok province were also conducted during october 1999-march 2000. the sputum specimens were collected and examined to identify paragonimus heterotremus eggs. in addition fecal samples were collected and examined for parasites by the formalin-ethyl acetate concentration technique. ... | 2001 | 12041605 |
| [experimental studies on development of paragonimus heterotremus in rats]. | to study the polymorphism of the development of paragonimus heterotremus in rats and to ascertain the host nature of the rat. | 1998 | 12078222 |
| [studies on the life cycle of paragonimus heterotremus]. | in order to clarify the biological features of the lung fluke, paragonimus heterotremus, it is necessary to replicate its complete life cycle in the laboratory. | 1998 | 12078271 |
| detection of opisthorchis viverrini in experimentally infected bithynid snails and cyprinoid fishes by a pcr-based method. | a pcr procedure for the detection of opisthorchis viverrini in experimentally infected bithynid snails and cyprinoid fishes was developed. this procedure was based on primers designed from a pov-a6 specific probe sequence giving a 330 base-pair product. the detection was accomplished in host tissue homogenates to which a single cercaria or metacercaria was introduced. pcr can detect as little as a single cercaria artificially inoculated in a snail or a single metacercaria artificially inoculated ... | 2003 | 12613764 |
| occurrence of the lung fluke, paragonimus heterotremus in manipur, india. | to determine the natural crustacean host, the paragonimus species, and to investigate the potential host-parasite relationship between manipur paragonimus and some of the laboratory animals. | 2003 | 12901523 |
| a rodent model for pulmonary paragonimiasis. | this study reports a model of pulmonary paragonimiasis in outbred wistar rats using the indian strain of paragonimus heterotremus isolated from the fresh water crab barytelphusa lugubris collected from arunachal pradesh, india. feeding metacercariae to the rats led to encapsulated adult worms in the lungs and pleural cavity. some flukes migrated to the skeletal muscles of the hosts where they remained underdeveloped as immature flukes. | 2003 | 14530967 |
| genomic characterization of lung flukes, paragonimus heterotremus, p. siamensis, p. harinasutai, p. westermani and p. bangkokensis by rapd markers. | random amplified polymorphic dna (rapd) markers were assayed in an attempt to discriminate among five species of paragonimus. genomic dnas of two strains of paragonimus heterotremus from two provinces in thailand, saraburi and phitsanulok, as well as of p. siamensis, p. harinasutai, p. westermani and p. bangkokensis were extracted and amplified by an arbitrary primer, namely p2 (5-gtttcgctcc-3). rapd patterns showed that those five species were genetically distinct, although they shared genomic ... | 2004 | 15350661 |
| detection of paragonimus heterotremus eggs in experimentally infected cats by a polymerase chain reaction-based method. | a polymerase chain reaction (pcr) procedure for the detection of paragonimus heterotremus eggs in stool samples was developed and compared with stoll's egg count method. the primers were designed on the basis of a previously constructed pph-13-specific dna probe, which produced an approximate 0.5-kb amplified product. this pcr method could detect as few as 5 eggs in 0.6 g of artificially inoculated feces of a healthy control cat or as little as 1 x 10(-4) ng of p. heterotremus genomic dna. the a ... | 2005 | 15856902 |
| molecular discrimination between individual metacercariae of paragonimus heterotremus and p. westermani occurring in thailand. | to accurately discriminate between individual metacercariae of paragonimus heterotremus and p. westermani occurring in thailand, polymerase chain reaction (pcr)-based molecular methods were established and subjected to an evaluation. we first amplified and sequenced the second internal transcribed spacer (its2) region of the nuclear ribosomal dna of the two species. based on their nucleotide differences, p. heterotremus and p. westermani were unequivocally discriminated from each other. these nu ... | 2005 | 16438190 |
| paragonimus heterotremus chen and hsia (1964), in vietnam: a molecular identification and relationships of isolates from different hosts and geographical origins. | paragonimus heterotremus chen and hsia (1964), and paragonimiasis caused by this species is a newly detected disease in vietnam. twelve samples of paragonimus (platyhelminthes: trematoda: digenea: paragonimidae) from different life-stages (eggs, miracidia, metacercariae, adults from natural and experimental hosts) and host species (crab, dog, cat and human) were collected in different geographical locations in vietnam. dna sequences were obtained from each for partial mitochondrial cytochrome c ... | 2006 | 16540074 |
| evaluation of human igg subclass antibodies in the serodiagnosis of paragonimiasis heterotremus. | immunoglobulin g subclass antibodies (igg1, igg2, igg3, and igg4) responses to the excretory-secretory antigens of the lung fluke, paragonimus heterotremus, were analyzed using the immunoblotting technique in an attempt to further improve the sensitivity and specificity for serodiagnosis of human paragonimiasis. serum samples from patients with proven paragonimiasis, from patients with other parasitic infections, pulmonary tuberculosis and from healthy counterparts were analyzed. the results ind ... | 2005 | 16572740 |
| pleuropulmonary paragonimiasis due to paragonimus heterotremus: molecular diagnosis, prevalence of infection and clinicoradiological features in an endemic area of northeastern india. | in the northeastern region of india, paragonimiasis is emerging as an important public health problem. however, until now the identity of the species causing human infection has been uncertain and there has been little information on the prevalence and clinicoradiological features of infection in the community. parasitological and immunological surveys revealed that paragonimiasis was hyperendemic in parts of arunachal pradesh. egg positivity in the sputum was 20.9% and 4.1% in children (age </= ... | 2007 | 17467757 |
| a new species of paragonimus (trematoda: troglotrematidae) from a cat infected with metacercariae from mountain crabs larnaudia larnaudii. | the identification of paragonimus spp. lung flukes is based on the morphology of both the metacercaria and the adult. a very small paragonimus sp. metacercaria was found in a freshwater crab caught in kanchanaburi province, west thailand, an area where metacercariae of paragonimus heterotremus had not been found. the metacercariae cysts were 180-204 microm in diameter, which was smaller than metacercariae of p. heterotremus. the coefficient of difference in body size between this metacercaria an ... | 2007 | 18314698 |
| identification of the human paragonimiasis causative agent in lao people's democratic republic. | to assess the species of human paragonimiasis in lao people's democratic republic, 6 ovum samples from 6 native confirmed paragonimiasis patients were examined with polymerase chain reaction (pcr) amplifying the internal transcribed spacer 2 (its2). the pcr products were sequenced, and a homology search was performed using the genbank. all 6 sequences were identical with paragonimus heterotremus its2. our work suggests that p. heterotremus may be the main etiological agent of human paragonimiasi ... | 2008 | 18973418 |
| paragonimus heterotremus infection in nagaland: a new focus of paragonimiasis in india. | to determine the prevalence of paragonimiasis among the patients who were attending the tuberculosis (tb) clinics at the community health centre, pfutsero, phek district, nagaland. to determine the species of paragonimus that cause infection in humans and the crustacean host that acts as the infectious source for humans. | 2009 | 19384034 |
| experimental infection with paragonimus heterotremus metacercariae in laboratory animals in manipur, india. | this study was aimed to find out the host-parasite relationship between paragonimus heterotremus isolated as metacercariae from mountain crabs, indochinamon manipurensis, in manipur, india and laboratory animals such as puppies, albino rats, swiss mice, guinea pigs, and rabbits, as experimental animals. the animals were fed with the metacercariae. infected animals were sacrificed 35 to 430 days after feeding to recover worms, which were used to determine the developmental stages. adult worms (n ... | 2011 | 21323162 |
| Human paragonimiasis in Viet Nam: epidemiological survey and identification of the responsible species by DNA sequencing of eggs in patients' sputum. | Parasitological and sero-epidemiological surveys for human paragonimiasis were conducted in three provinces of Viet Nam. A total of 590 participants from two known endemic areas of human paragonimiasis (Sinho district of Laichau province and Lucyen district of Yenbai province) and from Dakrong district of Quangtri province where we recently found crab hosts heavily infected with Paragonimus westermani metacercariae. By multiple dot-ELISA screening, 28 (12.7%) out of 220 participants in Sinho dis ... | 2011 | 21946337 |
| a singleplex real-time fluorescence resonance energy transfer pcr with melting curve analysis for the differential detection of paragonimus heterotremus, echinostoma malayanum and fasciola gigantica eggs in faeces. | because the eggs of paragonimus, echinostoma and fasciola are very similar in size and shape, it is difficult to distinguish and accurately identify species by the morphology of their eggs, which is a standard diagnostic method. | 2016 | 26740365 |
| molecular identification of paragonimus species by dna pyrosequencing technology. | dna pyrosequencing for pcr amplicons is an attractive strategy for the identification of microorganisms because of its short time performance for large number of samples. in this study, the primers targeting the fragment of its2 region of nuclear ribosomal rna gene were newly developed for pyrosequencing-based identification of 6 paragonimus species, paragonimus bangkokensis, paragonimus harinasutai, paragonimus heterotremus, paragonimus macrorchis, paragonimus siamensis and paragonimus westerma ... | 2013 | 23246361 |
| first case of paragonimus westermani infection in a female patient in india. | paragonimiasis is a foodborne parasitic zoonosis caused by lung fluke species of the genus paragonimus. the paragonimus westermani is the most common human pathogen in asian countries. in northeast india, paragonimus heterotremus has been documented as the only human pathogen in the earlier literature. in india, p. westermani infection in humans remained undetermined. herein, we report a case of pulmonary paragonimiasis due to p. westermani in an adult female in manipur. the diagnosis was made b ... | 2015 | 25657140 |
| massive pleural effusion due to paragonimiasis: biochemical, cytological, and parasitological findings. | paragonimiasis is an important food-borne parasitic zoonosis caused by trematode species of the genus, paragonimus occurring in many parts of the world except in australia and antarctica. in india, it is an emerging parasitic disease, which is endemic in the northeast states where people have a common practice of eating raw or inadequately cooked freshwater crabs. in these states, paragonimus heterotremus has been identified as the major causative agent of the human paragonimiasis. the most comm ... | 2014 | 25118756 |
| evaluation of igg4 subclass antibody detection by peptide-based elisa for the diagnosis of human paragonimiasis heterotrema. | a synthetic peptide was prepared based on the antigenic region of paragonimus westermani pre-procathepsin l, and its applicability for immunodiagnosis for human paragonimiasis (due to paragonimus heterotremus) was tested using an elisa to detect igg4 antibodies in the sera of patients. sera from other helminthiases, tuberculosis, and healthy volunteers were used as the references. this peptide-based assay system gave sensitivity, specificity, accuracy, and positive and negative predictive values ... | 2013 | 24516288 |
| molecular variation in the paragonimus heterotremus complex in thailand and myanmar. | paragonimiasis is an important food-borne parasitic zoonosis caused by infection with lung flukes of the genus paragonimus. of the 7 members of the genus known in thailand until recently, only p. heterotremus has been confirmed as causing human disease. an 8th species, p. pseudoheterotremus, has recently been proposed from thailand, and has been found in humans. molecular data place this species as a sister species to p. heterotremus, and it is likely that p. pseudoheterotremus is not specifical ... | 2013 | 24516273 |
| ultrastructure of spermatogenesis in the testis of paragonimus heterotremus. | lung fluke, paragonimus heterotremus, is a flatworm causing pulmonary paragonimiasis in cats, dogs, and humans in southeast asia. we examined the ultrastructure of the testis of adult p. heterotremus with special attention to spermatogenesis and spermiogenesis using scanning and transmission electron microscopy. the full sequence of spermatogenesis and spermiogenesis, from the capsular basal lamina to the luminal surface, was demonstrated. the sequence comprises spermatogonia, spermatocytes with ... | 2013 | 24516272 |
| paragonimus and paragonimiasis in vietnam: an update. | paragonimiasis is a food-borne parasitic zoonosis caused by infection with lung flukes of the genus paragonimus. in vietnam, research on paragonimus and paragonimiasis has been conducted in northern and central regions of the country. using a combination of morphological and molecular methods, 7 paragonimus species, namely p. heterotremus, p. westermani, p. skrjabini, p. vietnamensis, p. proliferus, p. bangkokenis and p. harinasutai, have been identified in vietnam. of these, the first 3, p. het ... | 2013 | 24516264 |
| metacercarial polymorphism and genetic variation of paragonimus heterotremus (digenea: paragonimidae), and a re-appraisal of the taxonomic status of paragonimus pseudoheterotremus. | paragonimus heterotremus, which is an important pathogen for human paragonimiasis in asia, is recognized as having the smallest metacercariae (maximum diameter < 300 μm) of any previously reported paragonimus species. recently, p. pseudoheterotremus has been described from thailand as a new species having metacercariae (about 200 μm) slightly smaller than those of thai p. heterotremus. in fact, the small size of p. pseudoheterotremus metacercariae is compatible with those of p. heterotremus from ... | 2015 | 24229574 |
| application of a real-time fluorescence resonance energy transfer polymerase chain reaction assay with melting curve analysis for the detection of paragonimus heterotremus eggs in the feces of experimentally infected cats. | paragonimus heterotremus is a medically important lung fluke that causes human and animal paragonimiasis in southeast asia, including thailand. in the current study, a real-time fluorescence resonance energy transfer polymerase chain reaction (real-time fret pcr) with melting curve analysis was developed and evaluated to detect p. heterotremus eggs in the feces of experimentally infected cats. the detection limit of this method for the p. heterotremus dna sequence was 3 × 10(2) copies of the pos ... | 2013 | 23897922 |
| pleuropulmonary paragonimiasis: mimicker of tuberculosis. | infection caused by the lung fluke is endemic in north eastern parts of india. paragonimus westermani and paragonimus heterotremus are known to be endemic in eastern indian states of manipur and nagaland. the infection is related to eating habits of the locals and is acquired by ingestion of raw, inadequately cooked crabs or crayfish containing encysted metacercariae which act as second intermediate hosts during the life cycle of the lung fluke. diagnosis is generally delayed due to lack of susp ... | 2013 | 23432864 |
| molecular identification of a case of paragonimus pseudoheterotremus infection in thailand. | paragonimiasis is an important food-borne parasitic zoonosis caused by infection with lung flukes of the genus paragonimus. in southeast asia, paragonimus heterotremus is the only proven causative pathogen. recently, a new paragonimus species, p. pseudoheterotremus, was found in thailand. this species is genetically similar to p. heterotremus and is considered as a sister species. however, infectivity or pathogenicity of p. pseudoheterotremus to humans remains unclear. we report the first confir ... | 2012 | 22826489 |