| characterization of borrelia lusitaniae sp. nov. by 16s ribosomal dna sequence analysis. | we determined the complete sequence of the rrs gene from five strains of genomic species potib2. both distance and parsimony methods were used to infer the evolutionary relationships of the rrs gene sequence of this genomic species in comparison with the rrs gene sequence of borrelia valaisiana and the rrs gene sequences of borrelia burgdorferi sensu lato species obtained from sequence databases. the phylogenetic analysis revealed that the genomic species potib2 strains clustered in a separate l ... | 1997 | 9336887 |
| infection of ixodes ricinus (acari: ixodidae) by borrelia burgdorferi sensu lato in north africa. | free-living adult ixodes ricinus l, were collected in amdoun, situated in the kroumiry mountains in northwestern tunisia (north africa). using direct fluorescence antibody assay, the infection rate of field-collected i. ricinus by borrelia burgdorferi sensu lato was 30.5% (n = 72). no difference in infection rate was observed between male and female ticks. spirochetes that had been isolated from i. ricinus from ain drahim (kroumiry mountains) in 1988 were identified as borrelia lusitaniae (forme ... | 1999 | 10083761 |
| molecular typing of borrelia burgdorferi sensu lato: taxonomic, epidemiological, and clinical implications. | borrelia burgdorferi sensu lato, the spirochete that causes human lyme borreliosis (lb), is a genetically and phenotypically divergent species. in the past several years, various molecular approaches have been developed and used to determine the phenotypic and genetic heterogeneity within the lb-related spirochetes and their potential association with distinct clinical syndromes. these methods include serotyping, multilocus enzyme electrophoresis, dna-dna reassociation analysis, rrna gene restri ... | 1999 | 10515907 |
| genetic diversity of borrelia burgdorferi sensu lato in ticks from mainland portugal. | to date borrelia lusitaniae is the only genospecies of borrelia burgdorferi sensu lato isolated from ixodes ricinus ticks collected in portugal and tunisia. this suggests that the genospecies diversity of b. burgdorferi sensu lato decreases toward the southwestern margin of its old world subtropical range. in order to further explore the genetic diversity of b. burgdorferi sensu lato from this region, 55 i. ricinus and 27 hyalomma marginatum questing adults, collected during the spring of 1998 f ... | 2000 | 10834965 |
| differentiation of borrelia burgdorferi sensu lato on the basis of rna polymerase gene (rpob) sequences. | we determined the nucleotide sequences (329 bp) of the rpob dnas from 22 reference strains of borrelia. no insertions or deletions were observed. deduced amino acid sequences of amplified rpob dna comprised 109 amino acid residues (n(450) to m(558) [escherichia coli numbering]). all amino acid sequences were identical with the exception of those of borrelia lusitaniae potib2 (t(461)-->a) and b. bissettii dn127 (i(498)-->v). each species of b. burgdorferi sensu lato was differentiated as a distin ... | 2000 | 10878043 |
| high prevalence of borrelia lusitaniae in ixodes ricinus ticks in tunisia. | to investigate whether ticks of the genus ixodes are infected by borrelia burgdorferi complex, 490 unfed ixodes ricinus ticks were collected by flagging in three different areas of tunisia in 1998. dnas extracted from 81 adults, 60 nymphs and 38 larvae were analysed after genic amplification of the non-coding spacer between the two copies of the rrl-rrf genes of b. burgdorferi sl. the prevalence of b. burgdorferi sl. in adults, nymphs and larvae was found to be 34, 33.3 and 2.6%, respectively. a ... | 2001 | 11523576 |
| a portuguese isolate of borrelia lusitaniae induces disease in c3h/hen mice. | a low-passage, portuguese isolate of borrelia lusitaniae, strain potib2, was inoculated into c3h/hen mice and disease was monitored by histopathology at 8 weeks after spirochaete challenge. ear, heart, bladder, femoro-tibial joint, brain and spinal cord were examined. b. lusitaniae strain potib2 (6 of 10 mice) and b. burgdorferi sensu stricto strain n40 (9 of 10 mice) induced similar lesions in the bladder of infected mice characterised as a multifocal, lymphoid, interstitial cystitis. moreover, ... | 2001 | 11761189 |
| horses and borrelia: immunoblot patterns with five borrelia burgdorferi sensu lato strains and sera from horses of various stud farms in austria and from the spanish riding school in vienna. | grazing animals are continuously exposed to tick bites. consequently, one may expect that horses will become infected with the various pathogens carried by ticks including borrelia burgdorferi sensu lato. whether horses may develop clinical disease due to this pathogen is controversially discussed. we were interested to learn about the infection of horses with borrelia burgdorferi sensu lato within one season by studying the dynamics of the humoral immune response in paired blood samples. the ma ... | 2002 | 12141766 |
| genetic variability within borrelia burgdorferi sensu lato genospecies established by pcr-single-strand conformation polymorphism analysis of the rrfa-rrlb intergenic spacer in ixodes ricinus ticks from the czech republic. | in europe the borrelia burgdorferi sensu lato complex is represented by five distinct genospecies: borrelia burgdorferi sensu stricto, borrelia afzelii, borrelia garinii, borrelia valaisiana, and borrelia lusitaniae. these taxonomic entities are known to differ in their specific associations with vertebrate hosts and to provoke distinct clinical manifestations in human patients. however, exceptions to these rules have often been observed, indicating that strains belonging to a single genospecies ... | 2003 | 12514035 |
| prevalence of borrelia burgdorferi species and identification of borrelia valaisiana in questing ixodes ricinus in the lyon region of france as determined by polymerase chain reaction-restriction fragment length polymorphism. | many cases of lyme borreliosis have been reported over the years in the region of lyon, france. the identification and prevalence of borrelia burgdorferi sensu lato in ixodes ricinus were investigated by polymerase chain reaction (pcr) of the flagellin gene and restriction fragment length polymorphism (rflp) analysis. questing ixodes ricinus larvae, nymphs and adults were collected by the flagging method from deciduous forests in four areas in the lyon region of france between october 1994 and s ... | 2003 | 12649714 |
| first isolation and characterization of borrelia burgdorferi sensu lato strains from ixodes ricinus ticks in turkey. | in order to investigate the presence and prevalence of lyme borreliosis (lyme disease) borrelia species, 312 unfed ticks were collected by flagging at a woodland area in trakya, in the european side of turkey, in may 2002. twelve of 299 ixodes ricinus ticks were infected with borrelia spp., as determined by cultivation in bsk medium (prevalence rate 4.0 %). ten pure cultures were subjected to further characterization by sequencing analysis of the 5s-23s rdna intergenic spacer, 16s rdna and flage ... | 2003 | 12909659 |
| first isolation of borrelia lusitaniae from a human patient. | the first human isolate of borrelia lusitaniae recovered from a portuguese patient with suspected lyme borreliosis is described. this isolate, from a chronic skin lesion, is also the first human isolate of borrelia in portugal. different phenotypic and molecular methods are used to characterize it. | 2004 | 15004107 |
| diversity within borrelia burgdorferi sensu lato genospecies in switzerland by reca gene sequence. | a total of 874 ixodes ricinus ticks were collected in switzerland to investigate the genetic diversity of the borrelia population. we integrated to the rt-pcr method the dna sequence analysis of a 162-bp fragment of the reca gene. five genospecies were detected: borrelia afzelii, borrelia burgdorferi s.s., borrelia garinii, borrelia valaisiana, and borrelia lusitaniae. a heterogeneous distribution was observed within the b. burgdorferi s.l. genospecies. the most prevalent and diverse genospecies ... | 2004 | 15336411 |
| first isolation of borrelia lusitaniae dna from ixodes ricinus ticks in poland. | european genospecies of b. burgdorferi sensu lato were identified by examining unfed i. ricinus ticks collected from 10 locations in northwest poland. research was conducted using 3 methods: pcr amplification of the fla gene with the fla1 and fla2 primer set conserved in all european species of b. burgdorferi sensu lato, pcr-rflp, and sequencing. there were 5 restriction patterns obtained in this study: 4 characteristic for genospecies b. burgdorferi sensu stricto, b. garinii, b. valaisiana, and ... | 2005 | 15764187 |
| characterization of borrelia lusitaniae isolates collected in tunisia and morocco. | borrelia lusitaniae is a species within the complex borrelia burgdorferi sensu lato and is infrequently isolated in europe. in contrast, this species is by far the most predominant in north africa and in portugal. in this study, we analyzed the genetic diversity, at several loci, of a large population of isolates from free-living ixodes ricinus ticks collected in tunisia and morocco. we found a moderate diversity of the whole genome by using pulsed-field gel electrophoresis as well as in the osp ... | 2005 | 15814970 |
| lyme borreliosis in portugal caused by borrelia lusitaniae? clinical report on the first patient with a positive skin isolate. | borrelia lusitaniae was isolated from an ixodes ricinus tick in portugal in 1993 for the first time. further, this borrelia genospecies has been found in ixodid ticks collected around the coasts of southern portugal and north africa. its reservoir has not been defined yet. b. lusitaniae was isolated once until now from a patient with a long standing and expanding skin disorder. | 2005 | 16053200 |
| prevalence of borrelia burgdorferi sensu lato in ticks collected from migratory birds in switzerland. | the prevalence of ticks infected by borrelia burgdorferi sensu lato on birds during their migrations was studied in switzerland. a total of 1,270 birds captured at two sites were examined for tick infestation. ixodes ricinus was the dominant tick species. prevalences of tick infestation were 6% and 18.2% for birds migrating northward and southward, respectively. borrelia valaisiana was the species detected most frequently in ticks, followed by borrelia garinii and borrelia lusitaniae. among bird ... | 2006 | 16391149 |
| borrelia lusitaniae and spotted fever group rickettsiae in ixodes ricinus (acari: ixodidae) in tuscany, central italy. | prevalence of infection by borrelia burgdorferi s.l. and spotted fever group (sfg) rickettsiae was estimated in host-seeking ticks in an area in tuscany, central italy, where lyme borreliosis was reported in a forestry worker. b. burgdorferi s.l. was identified by polymerase chain reaction in 16.7% (95% ci = 10.3, 24.8) of ixodes ricinus (l.) nymphs and 39.6% (95% ci = 26.5, 54.0) of adults. borrelia lusitaniae accounted for 82.9% of positive samples, followed by borrelia garinii (9.8%), borreli ... | 2006 | 16619594 |
| perpetuation of the lyme disease spirochete borrelia lusitaniae by lizards. | to determine whether the lyme disease spirochete borrelia lusitaniae is associated with lizards, we compared the prevalence and genospecies of spirochetes present in rodent- and lizard-associated ticks at a site where this spirochete frequently infects questing ticks. whereas questing nymphal ixodes ricinus ticks were infected mainly by borrelia afzelii, one-half of the infected adult ticks harbored b. lusitaniae at our study site. lyme disease spirochetes were more prevalent in sand lizards (la ... | 2006 | 16820453 |
| longitudinal analysis of tick densities and borrelia, anaplasma, and ehrlichia infections of ixodes ricinus ticks in different habitat areas in the netherlands. | from 2000 to 2004, ticks were collected by dragging a blanket in four habitat areas in the netherlands: dunes, heather, forest, and a city park. tick densities were calculated, and infection with borrelia burgdorferi and anaplasma and ehrlichia species was investigated by reverse line blot analysis. the lowest tick density was observed in the heather area (1 to 8/100 m2). in the oak forest and city park, the tick densities ranged from 26 to 45/100 m2. the highest tick density was found in the du ... | 2006 | 17028227 |
| borrelia lusitaniae and green lizards (lacerta viridis), karst region, slovakia. | in europe, spirochetes within the borrelia burgdorferi sensu lato complex are transmitted by ixodes ricinus ticks. specific associations are described between reservoir hosts and individual genospecies. we focused on green lizard (lacerta viridis) as a host for ticks and potential host for borreliae. in 2004 and 2005, a total of 146 green lizards infested by ticks were captured, and 469 i. ricinus ticks were removed. borrelial infection was detected in 16.6% of ticks from lizards. of 102 skin bi ... | 2006 | 17326941 |
| borrelia lusitaniae in immature ixodes ricinus (acari: ixodidae) feeding on common wall lizards in tuscany, central italy. | lizards and small rodents were live captured in tuscany, central italy, from may through august 2005. prevalence of infestation by larval ixodes ricinus l. (acari: ixodidae) and mean numbers of larvae per host were not significantly different for common wall lizards, podarcis muralis laurenti, and apodemus spp. mice, whereas infestation levels by nymphs were significantly greater on lizards. borrelia lusitaniae, which was previously shown to be dominant in host-seeking i. ricinus in the same stu ... | 2007 | 17427701 |
| borrelia lusitaniae ospa gene heterogeneity in mediterranean basin area. | in this study, borrelia lusitaniae dna extracted from ticks and lizards was used to amplify the outer surface protein a (ospa) gene in order to increase knowledge about sequence variability in the mediterranean basin area, to better understand how borrelia lusitaniae has evolved and how its distribution has expanded. phylogenetic trees including italian and reference sequences showed a clear separation of b. lusitaniae ospa strains in two different major clades. north african isolates form a cla ... | 2007 | 17896068 |
| diversity of ixodes ricinus tick-associated bacterial communities from different forests. | nymphal ixodes ricinus ticks (n=180) were collected from three different areas in the netherlands to investigate the effect of forest composition on tick-associated microbial communities. sampled habitats differed in thickness of leaf litter and humus layers and vegetation associations and were located near amsterdam (beech-oak), ede (birch-oak) and veldhoven (birch-oak). analysis of nine 16s rrna gene clone libraries made from individual ticks showed nearest matches with presumed pathogens cand ... | 2008 | 18355299 |
| detection of borrelia lusitaniae, rickettsia sp. irs3, rickettsia monacensis, and anaplasma phagocytophilum in ixodes ricinus collected in madeira island, portugal. | a total of 300 ixodes ricinus ticks were tested by polymerase chain reaction (pcr) for the presence of borrelia spp., rickettsia spp., and anaplasma phagocytophilum. sequence analysis demonstrated 8 (2.7%) ticks infected with b. lusitaniae, 60 (20%) with rickettsia spp., and 1 (0.3%) with a. phagocytophilum. seven (2.3%) ticks were coinfected with b. lusitaniae and rickettsia spp., 2 (0.6%) with r. monacensis, and 5 (1.7%) with rickettsia sp. irs3. the results of this study suggest simultaneous ... | 2008 | 18454598 |
| temporal variations in the usefulness of normalized difference vegetation index as a predictor for ixodes ricinus (acari: ixodidae) in a borrelia lusitaniae focus in tuscany, central italy. | host-seeking ticks were collected during monthly dragging sessions from november 2004 through october 2006 in tuscany, central italy. normalized difference vegetation index (ndvi), which was calculated from landsat etm+ 7 remote sensing data recorded in august 2001, was significantly correlated with numbers of host-seeking immature ixodes ricinus l. (acari: ixodidae) during periods of relatively low rainfall such as summer 2005 (spearman's p = 0.78, p < 0.001 for nymphs in july) and to a lower e ... | 2008 | 18533451 |
| characterization of borrelia burgdorferi sensu lato isolates by pulsed-field gel electrophoresis after mlui restriction of genomic dna. | at least three borrelia species (borrelia afzelii, borrelia garinii and borrelia burgdorferi sensu stricto) cause disease in humans, but borrelia spielmanii, borrelia valaisiana, borrelia lusitaniae and borrelia bissettii have also been reported to be rare or potential causes of human disease in europe. pulsed-field gel electrophoresis after mlui restriction of the genomic dna (mlui large restriction fragment patterns, lrfps) represents one of several approaches that have been used to assess bor ... | 2008 | 18586084 |
| vasculitis-like syndrome associated with borrelia lusitaniae infection. | we report the isolation of borrelia lusitaniae from a 13-year-old female child presenting with a vasculitis syndrome. the patient was treated with doxycycline, 100 mg bid for 20 days, and is in remission after a follow-up of 2 years. these results should alert clinicians to the fact that b. lusitaniae may be pathogenic in humans, highlighting that patients may be seronegative or present with minimal positive antibody titres and clinical signs that are not specific for lyme borreliosis. in order ... | 2008 | 18795392 |
| fine-scale phylogeographic structure of borrelia lusitaniae revealed by multilocus sequence typing. | borrelia lusitaniae is an old world species of the lyme borreliosis (lb) group of tick-borne spirochetes and prevails mainly in countries around the mediterranean basin. lizards of the family lacertidae have been identified as reservoir hosts of b. lusitaniae. these reptiles are highly structured geographically, indicating limited migration. in order to examine whether host geographic structure shapes the evolution and epidemiology of b. lusitaniae, we analyzed the phylogeographic population str ... | 2008 | 19104655 |
| molecular detection of borrelia bissettii dna in serum samples from patients in the czech republic with suspected borreliosis. | until recently, three spirochete genospecies were considered to be the causative agents of lyme borreliosis (lb) in europe: borrelia burgdorferi sensu stricto, borrelia afzelii and borrelia garinii. however, the dna of borrelia valaisiana, borrelia lusitaniae, borrelia spielmanii and borrelia bissettii has already been detected in samples of human origin, or the spirochetes were isolated from the patients with symptoms of lb. molecular analysis of 12 selected serum samples collected in the regio ... | 2009 | 19187198 |
| detection of borrelia burgdorferi sensu lato in lizards and their ticks from hungary. | to investigate the involvement of lizard species in the natural cycle of borrelia burgdorferi sensu lato (s.l.) in hungary, a total of 186 reptiles belonging to three species--126 green lizards (lacerta viridis), 40 balkan wall lizards (podarcis taurica), and 20 sand lizards (lacerta agilis)--were captured in 2007 and 2008. all ticks removed from the lizards were ixodes ricinus, either larvae (324/472; 68.6%) or nymphs (148/472; 31.4%). more than half (66/126; 52.4%) of l. viridis individuals we ... | 2009 | 19492950 |
| molecular characterization of a new isolate of borrelia lusitaniae derived from apodemus sylvaticus in portugal. | a total of 196 small mammals were collected in portugal and tested for borrelia burgdorferi sensu lato. tissue samples were taken from each animal and cultured in barbour-stoenner-kelly (bsk)-ii medium. the single strain of spirochete isolated was confirmed as borrelia lusitaniae by genetic analyses. this is the first report of b. lusitaniae isolated from apodemus sylvaticus. | 2010 | 19725761 |
| transmission dynamics of borrelia lusitaniae and borrelia afzelii among ixodes ricinus, lizards, and mice in tuscany, central italy. | to estimate the basic reproduction number (r(0)) of borrelia lusitaniae and borrelia afzelii, we formulated a mathematical model considering the interactions among the tick vector, vertebrate hosts, and pathogens in a 500-ha enclosed natural reserve on le cerbaie hills, tuscany, central italy. in the study area, ixodes ricinus were abundant and were found infected by b. lusitaniae and b. afzelii. lizards (podarcis spp.) and mice (apodemus spp.), respectively, are the reservoir hosts of these two ... | 2011 | 20482342 |
| associations between coinfection prevalence of borrelia lusitaniae, anaplasma sp., and rickettsia sp. in hard ticks feeding on reptile hosts. | an increasing number of studies reveal that ticks and their hosts are infected with multiple pathogens, suggesting that coinfection might be frequent for both vectors and wild reservoir hosts. whereas the examination of associations between coinfecting pathogen agents in natural host-vector-pathogen systems is a prerequisite for a better understanding of disease maintenance and transmission, the associations between pathogens within vectors or hosts are seldom explicitly examined. we examined th ... | 2011 | 20711724 |
| inadequate binding of immune regulator factor h is associated with sensitivity of borrelia lusitaniae to human complement. | spirochetes belonging to the borrelia burgdorferi sensu lato complex differ in resistance to complement-mediated killing by human serum. here, we characterize complement sensitivity of a panel of b. lusitaniae isolates derived from ticks collected in germany and portugal as well as one patient-derived isolate, pohl. all isolates are highly susceptible to complement-mediated lysis in human serum and activate complement predominantly by the alternative pathway, leading to an increased deposition o ... | 2010 | 20823202 |
| evaluation of a real-time pcr assay for the detection, genotyping, and quantification of borrelia burgdorferi sensu lato in ixodes ticks in a routine laboratory setting. | testing of saved ixodes ticks for the presence of borrelia burgdorferi after biting a patient may be helpful to assess the risk of bacterial transmission and to decide upon preventive antibiotic treatment. a commercially available real-time pcr assay for the identification and quantification of borrelia burdorferi sensu lato species (lightmix kit for the detection of borrelia spp., tib molbiol) was evaluated. | 2011 | 21391467 |
| the expanding lyme borrelia complex--clinical significance of genomic species? | ten years after the discovery of spirochaetes as agents of lyme disease in 1982 in the usa, three genomic species had diverged from the phenotypically heterogeneous strains of borrelia burgdorferi isolated in north america and europe: borrelia afzelii, b. burgdorferi sensu stricto (further b. burgdorferi), and borrelia garinii. whereas b. burgdorferi remained the only human pathogen in north america, all three species are aetiological agents of lyme borreliosis in europe. another seven genospeci ... | 2011 | 21414082 |
| correlation of borrelia burgdorferi sensu lato prevalence in questing ixodes ricinus ticks with specific abiotic traits in the western palearctic. | this meta-analysis of reports examining ticks throughout the western palearctic region indicates a distinct geographic pattern for borrelia burgdorferi sensu lato prevalence in questing nymphal ixodes ricinus ticks. the greatest prevalence was reported between the 5°e and 25°e longitudes based on an analysis of 123 collection points with 37,940 nymphal tick specimens (87.43% of total nymphs; 56.35% of total ticks in the set of reports over the target area). climatic traits, such as temperature a ... | 2011 | 21498767 |
| plasmid profile analysis of portuguese borrelia lusitaniae strains. | plasmid profiles of 2 portuguese borrelia lusitaniae strains, one isolated from a human patient and the other one from an ixodes ricinus tick, were obtained by pulsed-field gel electrophoresis to evaluate the plasmid diversity in each strain. overall, a maximum of 6 plasmids were detected that ranged from 19 kb to 76 kb, revealing completely different plasmid profiles from those previously described for other genospecies of b. burgdorferi sensu lato, the causative agents of lyme borreliosis. the ... | 2010 | 21771519 |
| coinfections of rickettsia slovaca and rickettsia helvetica with borrelia lusitaniae in ticks collected in a safari park, portugal. | borrelia and rickettsia bacteria are the most important tick-borne agents causing disease in portugal. identification and characterization of these circulating agents, mainly in recreational areas, is crucial for the development of preventive measures in response to the gradually increasing exposure of humans to tick vectors. a total of 677 questing ticks including dermacentor marginatus, rhipicephalus sanguineus, ixodes ricinus, hyalomma lusitanicum, h. marginatum, and haemaphysalis punctata we ... | 2010 | 21771525 |
| infections and coinfections of questing ixodes ricinus ticks by emerging zoonotic pathogens in western switzerland. | in europe, ixodes ricinus is the vector of many pathogens of medical and veterinary relevance, among them borrelia burgdorferi sensu lato and tick-borne encephalitis virus, which have been the subject of numerous investigations. less is known about the occurrence of emerging tick-borne pathogens like rickettsia spp., babesia spp., "candidatus neoehrlichia mikurensis," and anaplasma phagocytophilum in questing ticks. in this study, questing nymph and adult i. ricinus ticks were collected at 11 si ... | 2012 | 22522688 |
| role of the lizard teira dugesii as a potential host for ixodes ricinus tick-borne pathogens. | pcr screening of ticks and tissue samples collected from 151 teira dugesii lizards seems to indicate a potential role of this lizard species in the maintenance and transmission cycle of some ixodes ricinus tick-borne agents, such as rickettsia monacensis, rickettsia helvetica, and borrelia lusitaniae, that are circulating on madeira island. | 2012 | 22407681 |
| borrelia burgdorferi sensu lato and co-infections with anaplasma phagocytophilum and rickettsia spp. in ixodes ricinus in hamburg, germany. | to obtain initial data on borrelia burgdorferi sensu lato (spirochaetales: spirochaetaceae) in ixodes ricinus (ixodida: ixodidae) ticks in hamburg, germany, 1400 questing ticks were collected by flagging at 10 different public recreation areas in 2011 and analysed using probe-based quantitative real-time polymerase chain reaction. the overall rate of infection with b. burgdorferi s.l. was 34.1%; 30.0% of adults were infected (36.7% of females and 26.0% of males), as were 34.5% of nymphs. signifi ... | 2015 | 26096626 |
| imbalanced presence of borrelia burgdorferi s.l. multilocus sequence types in clinical manifestations of lyme borreliosis. | in this study we used typing based on the eight multilocus sequence typing scheme housekeeping genes (mlst) and 5s-23s rdna intergenic spacer (igs) to explore the population structure of borrelia burgdorferi sensu lato isolates from patients with lyme borreliosis (lb) and to test the association between the b. burgdorferi s.l. sequence types (st) and the clinical manifestations they cause in humans. isolates of b. burgdorferi from 183 lb cases across europe, with distinct clinical manifestations ... | 2016 | 27125686 |
| natural foci of borrelia lusitaniae in a mountain region of central europe. | lyme borreliosis is the most prevalent tick-borne disease in europe. it is caused by spirochaetes of the borrelia burgdorferi sensu lato (s.l.) complex and transmitted to humans by ticks of the genus ixodes. borrelia afzelii, borrelia garinii, and borrelia valaisiana are the most common genospecies in central europe. in contrast, borrelia lusitaniae predominates in mediterranean countries such as portugal, morocco, and tunisia. in slovakia, its prevalence is low and restricted to only a few site ... | 2016 | 26711673 |
| spatial stratification of various lyme disease spirochetes in a central european site. | to determine whether the genospecies composition of lyme disease spirochetes is spatially stratified, we collected questing ixodes ricinus ticks in neighboring plots where rodents, birds, and lizards were present as reservoir host and compared the prevalence of various genospecies. the overall prevalence of spirochetes in questing ticks varied across the study site. borrelia lusitaniae appeared to infect adult ticks in one plot at the same frequency as did borrelia afzelii in the other plots. th ... | 2013 | 23062207 |
| molecular identification of borrelia genus in questing hard ticks from portugal: phylogenetic characterization of two novel relapsing fever-like borrelia sp. | in the last decades, several studies have reported pathogenic species of borrelia related to those that cause tick-borne relapsing fever (rf), but unexpectedly suggesting their transmission by hard ticks, known vectors of borrelia burgdorferi sensu lato (b. burgdorferi s.l.) species, rather than by soft ticks. this study was designed to update the presence of b. burgdorferi s.l. species in ticks from several districts of mainland portugal, where ixodes ricinus had been previously described. tick ... | 2016 | 26976475 |
| occurrence of borrelia lusitaniae infection in horses. | the aim of the study was to investigate borrelia burgdorferi sensu lato (s.l.) infection in horses exposed to heavy tick infestations. blood samples of 98 healthy horses from 5 stud farms were examined by snap(®) 4d× and pcr to detect antibodies against b. burgdorferi s.l. and borrelia dna, respectively. ten samples (15.3%) were antibody positive and 5 samples (5.1%) were both antibody and pcr positive. sequence analysis showed the highest homology with the b. lusitaniae genospecies. no differen ... | 2012 | 22789679 |
| the importance of lizards and small mammals as reservoirs for borrelia lusitaniae in portugal. | borrelia lusitaniae is a pathogen frequent in the mediterranean area. apart from lizards, evidence for birds and small mammals as competent reservoirs for this genospecies has been occasional. we collected questing ticks, skin biopsies and ixodes sp. ticks feeding on lizards, birds and small mammals in a b. burgdorferi s.l. (sensu lato) enzootic area to assess their importance in the maintenance of b. lusitaniae. borrelia lusitaniae was the most prevalent genospecies in questing ticks and was co ... | 2015 | 25291988 |
| modeling the effects of variable feeding patterns of larval ticks on the transmission of borrelia lusitaniae and borrelia afzelii. | spirochetes belonging to the borrelia burgdoferi sensu lato (sl) group cause lyme borreliosis (lb), which is the most commonly reported vector-borne zoonosis in europe. b. burgdorferi sl is maintained in nature in a complex cycle involving ixodes ricinus ticks and several species of vertebrate hosts. the transmission dynamics of b. burgdorferi sl is complicated by the varying competence of animals for different genospecies of spirochetes that, in turn, vary in their capability of causing disease ... | 2017 | 28690096 |
| approaches for reverse line blot-based detection of microbial pathogens in ixodes ricinus ticks collected in austria and impact of the chosen method. | ticks transmit a large number of pathogens capable of causing human disease. in this study, the pcr-reverse line blot (rlb) method was used to screen for pathogens in a total of 554 ixodes ricinus ticks collected from all provinces of austria. these pathogens belong to the genera borrelia, rickettsiae, anaplasma/ehrlichia (including "candidatus neoehrlichia"), babesia, and coxiella the pathogens with the highest detected prevalence were spirochetes of the borrelia burgdorferisensu lato complex, ... | 2017 | 28455331 |