| epidemiology of human visceral leishmaniasis in taounate, a northern province of morocco. | we report the preliminary results of an epidemiological survey carried out in the locality of ouled hcein, province of taounate (north morocco) around one visceral leishmaniasis infant case. the strain isolated from the child was characterized by three methods as leishmania infantum and its isoenzyme pattern was indistinguishable from zymodeme mon-1. the human survey in the locality of origin of the index case did not show any additional case and the serology was negative for the whole people sa ... | 1997 | 9296060 |
| morphological and isoenzymatic differentiation of sympatric populations of phlebotomus perniciosus and phlebotomus longicuspis (diptera: psychodidae) in northern morocco. | the phlebotomine sandfly phlebotomus perniciosus newstead is a vector of leishmania infantum nicolle in the western mediterranean basin. in northern morocco it is often caught in sympatry with a closely related species, p. longicuspis nitzulescu. in the locality of chefchaouene where females of both species were morphologically distinguishable, none of the collected males exhibited forked copulatory valves, which is considered a specific character for p. perniciosus. they 1st were identified as ... | 1999 | 10071503 |
| genetic polymorphism in sympatric species of the genus phlebotomus, with special reference to phlebotomus perniciosus and phlebotomus longicuspis (diptera, phlebotomidae). | the random amplified polymorphic dna assay was used to study genetic variation within and between five phlebotomus species belonging to three subgenera: p. (larroussius) ariasi, p. (l.) longicuspis, p. (l.) perniciosus, p.(paraphlebotomus) sergenti and p. (phlebotomus) papatasi sympatric in southern spain and proven vector of leishmaniasis. two cluster analysis were proposed: one according to sandfly species and populations, the second according individual specimens of phlebotomus perniciosus, p ... | 2000 | 11147032 |
| distribution of phlebotomine sandflies, of the subgenus larroussius, in morocco. | adult sandflies (diptera: psychodidae) of the genus phlebotomus and of the subgenus larroussius were collected with sticky traps from thirty-four localities in central and south-western morocco. four larroussius species were present in this area, phlebotomus perniciosus newstead, being the most widespread and abundant species particularly in the mountainous area, was sympatric with the closely related species phlebotomus longicuspis nitzulescu. only one male of p. perniciosus displayed the bifid ... | 2005 | 15752186 |
| morphological and isoenzymatic differentiation of phlebotomus perniciosus and phlebotomus longicuspis (diptera: psychodidae) in southern morocco. | morphological and enzymatic characterization of phlebotomus perniciosus and phlebotomus longicuspis in morocco is reported. twenty-nine localities in central and southern of morocco were sampled and compared with three localities from the rif (northern morocco). for morphological study, sand flies were collected by sticky-paper traps. for males, the morphology of the copulatory valves (aedeagi) was examined and the number of coxite hairs was recorded. for isoenzyme analyses, specimens were colle ... | 2008 | 18456222 |
| the phlebotomine fauna (diptera: psychodidae) of the eastern coast of tunisia. | to identify the phlebotomine sand fly populations of the eastern coast of tunisia, an entomological survey was carried out between september and october 2005 at 71 sites located in three districts. cdc light traps and sticky papers were used to collect a total of 2,138 phlebotomine sand flies representing nine species. the predominant species occurring on the eastern coast of tunisia are, in order of abundance, phlebotomus longicuspis nitzulescu, 1930 (40%); phlebotomus papatasi scopoli, 1786 (2 ... | 2009 | 19198511 |
| punique virus, a novel phlebovirus, related to sandfly fever naples virus, isolated from sandflies collected in tunisia. | sandflies are widely distributed around the mediterranean basin. therefore, human populations in this area are potentially exposed to sandfly-transmitted diseases, including those caused by phleboviruses. whilst there are substantial data in countries located in the northern part of the mediterranean basin, few data are available for north africa. in this study, a total of 1489 sandflies were collected in 2008 in tunisia from two sites, bioclimatically distinct, located 235 km apart, and identif ... | 2010 | 20089800 |
| molecular and serological evidence for the presence of novel phleboviruses in sandflies from northern algeria. | during summer 2007, a total of 785 phlebotomine flies were trapped in northern algeria, identified morphologically, organised as monospecific pools and tested for the presence of phlebovirus rna using degenerate primers. three pools were positive, and the corresponding pcr products were cloned and sequenced. viral sequences corresponding to two phleboviruses distinct from each other were detected in sandflies circulating in two close locations (140 km apart) in northern algeria. the 3 sequences ... | 2010 | 20563287 |
| no recent adaptive selection on the apyrase of mediterranean phlebotomus: implications for using salivary peptides to vaccinate against canine leishmaniasis. | vaccine development is informed by a knowledge of genetic variation among antigen alleles, especially the distribution of positive and balancing selection in populations and species. a combined approach using population genetic and phylogenetic methods to detect selective signatures can therefore be informative for identifying vaccine candidates. parasitic leishmania species cause the disease leishmaniasis in humans and mammalian reservoir hosts after inoculation by female phlebotomine sandflies ... | 2011 | 25568049 |
| ecological status of phlebotomine sandflies (diptera: psychodidae) in rural communities of northeastern algeria. | algeria is among the most affected mediterranean countries by leishmaniasis due to its large geographic extent and climatic diversity. the current study aimed to determine the ecological status (composition and diversity) of phlebotomine sandfly populations in the region of oum el bouaghi (northeast algeria). | 2015 | 27047969 |
| limits of a rapid identification of common mediterranean sandflies using polymerase chain reaction-restriction fragment length polymorphism. | a total of 131 phlebotomine algerian sandflies have been processed in the present study. they belong to the species phlebotomus bergeroti, phlebotomus alexandri, phlebotomus sergenti, phlebotomus chabaudi, phlebotomus riouxi, phlebotomus perniciosus, phlebotomus longicuspis, phlebotomus perfiliewi, phlebotomus ariasi, phlebotomus chadlii, sergentomyia fallax, sergentomyia minuta, sergentomyia antennata, sergentomyia schwetzi, sergentomyia clydei, sergentomyia christophersi and grassomyia dreyfus ... | 2014 | 24936911 |
| spatial patterns and secular trends in human leishmaniasis incidence in morocco between 2003 and 2013. | few studies on spatial patterns or secular trends in human leishmanias have been conducted in morocco. this study aimed to examine spatial patterns and trends associated with the human leishmaniasis incidence rate (hlir) at the province/prefecture level between 2003 and 2013 in morocco. | 2016 | 27164836 |
| changes of sand fly populations and leishmania infantum infection rates in an irrigated village located in arid central tunisia. | the current spread of zoonotic visceral leishmaniasis (zvl) throughout arid areas of central tunisia is a major public health concern. the main objective of this study is to investigate whether the development of irrigation in arid bio-geographical areas in central tunisia have led to the establishment of a stable cycle involving sand flies of the subgenus larroussius and leishmania infantum, and subsequently to the emergence of zvl. sand flies were collected from the village of saddaguia, a hig ... | 2016 | 26999176 |
| species composition of sand flies and bionomics of phlebotomus papatasi and p. sergenti (diptera: psychodidae) in cutaneous leishmaniasis endemic foci, morocco. | cutaneous leishmaniasis (cl) is one of the most neglected tropical diseases in morocco. leishmania major and l. tropica are the main culprits identified in all endemic foci across the country. these two etiological agents are transmitted by phlebotomus papatasi and p. sergenti, the two most prevalent sand fly species in morocco. previous studies reflected gaps of knowledge regarding the environmental fingerprints that affect the distribution of these two potential vectors across morocco. | 2016 | 26830689 |
| toscana virus isolated from sandflies, morocco. | to investigate the transmission of phleboviruses, a total of 7,057 sandflies were collected in well-known foci of cutaneous leishmaniasis and were identified to species level according to morphological characters.collected sandflies were tested by nested pcr for the presence of phleboviruses and subsequently by viral isolation on vero cells. the corresponding products were sequenced. toscana virus was isolated, for the first time, from 5 pools of sandflies.hence, toscana virus should be consider ... | 2015 | 25886511 |
| leishmaniasis in central morocco: seasonal fluctuations of phlebotomine sand fly in aichoun locality, from sefrou province. | cutaneous leishmaniases (cl) are endemic in morocco. they are common in the human population in different localities such as aichoun in sefrou province, morocco. this study was carried out in aichoun locality from april to october 2012 in order to study the spatiotemporal trends of the main leishmania phlebotomine vectors in this focus. overall, 1171 sand flies, belonging to four species, were collected by sticky traps. phlebotomus sergenti was the predominant species (78.4%) followed by ph. per ... | 2015 | 25741448 |
| phlebovirus and leishmania detection in sandflies from eastern thrace and northern cyprus. | phlebotomine sandflies are vectors of several pathogens with significant impact for public health. this study was conducted to investigate and characterize phlebovirus and leishmania infections in vector sandflies collected in the eastern thrace region in turkey and northern cyprus, where previous data indicate activity of these agents. | 2014 | 25499083 |
| irrigation in the arid regions of tunisia impacts the abundance and apparent density of sand fly vectors of leishmania infantum. | the distribution expansion of important human visceral leishmaniasis (hvl) and sporadic cutaneous leishmaniasis (scl) vector species, phlebotomus perfiliewi and p. perniciosus, throughout central tunisia is a major public health concern. this study was designed to investigate if the expansion of irrigation influences the abundance of sand fly species potentially involved in the transmission of hvl and scl located in arid bioclimatic regions. geographic and remote sensing approaches were used to ... | 2014 | 25447265 |
| characterization of the sandfly fever naples species complex and description of a new karimabad species complex (genus phlebovirus, family bunyaviridae). | genomic and antigenic characterization of members of the sandfly fever naples virus (sfnv) complex reveals the presence of five clades that differ in their geographical distribution. saint floris and gordil viruses, both found in africa, form one clade; punique, granada and massilia viruses, all isolated in the western mediterranean, constitute a second; toscana virus, a third; sfnv isolates from italy, cyprus, egypt and india form a fourth; while tehran virus and a serbian isolate yu 8/76, repr ... | 2013 | 24096318 |
| detection of wolbachia pipientis, including a new strain containing the wsp gene, in two sister species of paraphlebotomus sandflies, potential vectors of zoonotic cutaneous leishmaniasis. | individual, naturally occurring phlebotomus mongolensis and phlebotomus caucasicus from iran were screened for infections with the maternally inherited intracellular rickettsia-like bacterium wolbachia pipientis via targeting a major surface protein gene (wsp). the main objective of this study was to determine if w. pipientis could be detected in these species. the sandflies were screened using polymerase chain reaction to amplify a fragment of the wolbachia surface protein gene. the obtained se ... | 0 | 23828002 |
| molecular detection and identification of leishmania infection in naturally infected sand flies in a focus of cutaneous leishmaniasis in northern morocco. | cutaneous leishmaniasis is an infectious disease caused by various species of the flagellate protozoan leishmania. during the past 20 years, cutaneous leishmaniasis has emerged as a major public health threat in morocco. the main objective of this study was to study the occurrence of leishmania infection in vectors and to identify sand fly blood meal sources in an endemic locality of cutaneous leishmaniasis within sefrou province, where the vectors of leishmaniasis were still unknown. | 2014 | 24990497 |
| new epidemiological aspects of visceral and cutaneous leishmaniasis in taza, morocco. | leishmaniasis is considered among the main endemic diseases in morocco. however, further knowledge about epidemiological aspects of this disease is needed in several provinces to plan control and preventive strategies to tackle the disease. the present study aims to determine the epidemiological aspect of cutaneous and visceral leishmaniasis in taza province from 2007-2014 and to identify the circulating species in this province. | 2016 | 27899126 |
| presence of sandfly-borne phleboviruses of two antigenic complexes (sandfly fever naples virus and sandfly fever sicilian virus) in two different bio-geographical regions of tunisia demonstrated by a microneutralisation-based seroprevalence study in dogs. | sandfly-borne phleboviruses are present in north africa where they can infect humans in regions where leishmania infantum, the causative agent of zoonotic visceral leishmaniasis in the western mediterranean basin is present affecting both humans and dogs. we investigated the capacity of dogs to be used as sentinels for sandfly-borne phleboviruses as previously shown for leishmaniasis. | 2014 | 25306250 |
| co-circulation of toscana virus and punique virus in northern tunisia: a microneutralisation-based seroprevalence study. | in northern tunisia, the co-circulation of two related sand fly-borne phleboviruses, toscana virus (tosv) and punique virus (punv) was previously demonstrated. in contrast to tosv, a prominent human pathogen, there is no data supporting that punv is capable to infect and cause disease to humans. we studied the respective involvement of tosv and punv in human infections in northern tunisia through a seroprevalence study. | 2013 | 24069484 |
| infection of sand flies collected from different bio-geographical areas of tunisia with phleboviruses. | an entomological investigation performed in 2013 covering different bio-geographical areas varying from humid in the north to the arid in the center showed that sand flies of the subgenus larroussius including phlebotomus perniciosus, phlebotomus perfiliewi, and phlebotomus longicuspis are abundant and widely distributed in tunisia. a total of 3992 collected and pooled with up to 30 specimens per pool based on sex, trapping location and collection data were tested for the presence of phlebovirus ... | 2015 | 25255967 |
| ecological and epidemiological status of species of the phlebotomus perniciosus complex (diptera: psychodidae, phlebotominae) in morocco. | leishmania infantum (kinetoplastida: trypanosomatidae) infection is transmitted by an infected female sand fly (diptera: psychodidae, phlebotominae) of the subgenus larroussius: phlebotomus ariasi, phlebotomus perniciosus, and phlebotomus longicuspis in the mediterranean basin. in morocco, the vectorial role of p. ariasi was demonstrated, while that of p. longicuspis and p. perniciosus is not elucidated. in addition, moroccan p. longicuspis and p. perniciosus populations present a higher morphol ... | 2016 | 26593735 |
| species composition of phlebotomine sand fly fauna in an area with sporadic cases of leishmania infantum human visceral leishmaniasis, morocco. | visceral and cutaneous leishmaniases are the main endemic vector-born diseases in morocco. human visceral leishmaniasis (hvl), by leishmania infantum, currently presents a significant health problem throughout the country and may constitute factor for death, especially among children with less than 15 years old. in the past, hvl has been basically absent or at least sporadic in marrakesh-tensift-al haouz region; however it became significant during the last decade. an entomological survey and a ... | 2015 | 25930188 |
| interaction between canine and human visceral leishmaniases in a holoendemic focus of central tunisia. | canine visceral leishmaniasis (cvl) is endemic in the mediterranean basin. in tunisia, cvl is spatially associated with human visceral leishmaniasis (hvl) affecting mostly children younger than 5 years old. in this study, seroprevalence of leishmania infantum infection in dogs was assessed in highly endemic districts of the governorate of kairouan where more than 50% of hvl cases in tunisia were reported. an entomological investigation was also carried out in two endemic districts (bouhajla and ... | 2014 | 25004438 |
| first detection of leishmania infantum dna in phlebotomus longicuspis nitzulescu, 1930 from visceral leishmaniasis endemic focus in algeria. | two clinico-epidemiological forms of leishmaniasis caused by leishmania infantum complex are endemic in algeria: human visceral leishmaniasis (hvl) and sporadic cutaneous leishmaniasis. in the northern part of the country, the kabylian region is the main endemic hvl focus with more than 200 cases recorded annually. during the summer of 2009, an entomological study was performed in larbaa nath irathen with the aim to identify the vectors of leishmania and of phleboviruses. in the present paper, w ... | 2012 | 22350715 |
| phleboviruses associated with sand flies in arid bio-geographical areas of central tunisia. | an entomological investigation was carried out in 2014 at two sites located in central tunisia, one irrigated and another non-irrigated situated in arid bio-geographical areas. sand flies of the subgenus larroussius namely phlebotomus perfiliewi, phlebotomus perniciosus, and phlebotomus longicuspis are the most abundant sand fly species in the irrigated site. however, in the non-irrigated site, phlebotomus papatasi of the phlebotomus genus is the most abundant species. a total of 3191 sand flies ... | 2016 | 26875761 |
| presence of phlebotomus perniciosus atypical form in algeria. | phlebotomus perniciosus and phlebotomus longicuspis are two phlebotomine sand fly species morphologically similar and differing in males only by the shape of the copulatory valves which are bifurcated in p. perniciosus, tip long and tapered in p. longicuspis. | 2017 | 29026861 |
| morphological and molecular investigations of population structure of phlebotomus perniciosus and phlebotomus longicuspis (diptera: psychodidae) in tunisia. | morphological and molecular characterization of phlebotomus (larroussius) perniciosus newstead and phlebotomus (l.) longicuspis nitzulescu in tunisia is reported. different localities in central and southern tunisia were sampled. sand flies were collected by sticky-paper traps and center for disease control traps. for morphological study of males, the copulatory valves (aedeagi) were examined and the number of coxite hairs was recorded. for molecular analysis, the mitochondrial cytochrome b gene ... | 2012 | 22897038 |