Publications

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[fine structure of cyst and cyst wall of sarcocystis tenella, besnoitia jellisoni, frenkelia sp. and toxoplasma gondii]. 19744209540
quinine inhibition of host cell penetration by toxoplasma gondii, besnoitia jellisoni, and sarcocystis sp. in vitro. 19724625324
electron microscope study of the proliferative form of besnoitia jellisoni. 19664957507
the function of the micropyle in the cyst organisms of besnoitia jellisoni. 19674962343
observations on the fine structure of the "cyst stage" of besnoitia jellisoni. 19684976001
cultivation of besnoitia jellisoni in bovine cell cultures. 19694978146
[on the ultrastructure of cysts of besnoitia jellisoni--frenkel 1953--(sporozoa, toxoplasmea) in mice (mus musculus)]. 19694980390
attachment of toxoplasma gondii to host cells involves major surface protein, sag-1 (p30).previous observations have demonstrated that monoclonal and polyclonal antibodies directed at sag-1, the major surface protein of toxoplasma gondii, decreased the number of t. gondii that infected fibroblast monolayers. direct evaluation of parasite-host cell attachment using glutaraldehyde-fixed human fibroblasts and live tachyzoites was performed to determine whether sag-1 was a ligand for the host cell receptor. the interaction between the fixed cells and t. gondii was specific and saturable ...19948050521
comparative antigenic study of besnoitia jellisoni, b. panamenis and five toxoplasma gondii isolates. 19684969527
a role for activated macrophages in resistance to infection with toxoplasma.activated macrophages from mice which were chronically infected with toxoplasma gondii or besnoitia jellisoni, or which had received freund complete adjuvant, had an enhanced capacity to to kill intracellular toxoplasma. enhanced killing by activated macrophages was demonstrated by decreased incorporation of isotopically labeled uridine by intracellular toxoplasma and by inhibition of plaque formation. the latter resulted from lack of proliferation of the intracellular toxoplasma which is normal ...19724637298
[cytochemical studies on besnoitia jellisoni cysts. distribution of acid phosphatase and polysaccharide at the ultrastructural level]. 19724344752
besnoitia jellisoni in macrophages and cysts from experimentally infected laboratory mice. 19744217372
specific and nonspecific mediation of protective immunity to toxoplasma gondii.we studied the specificity of protection conferred by toxoplasma gondii immune lymphocytes and their supernatants on infected hamster kidney cells, using besnoitia jellisoni immune lymphocytes as a nonspecific control. the intracellular growth of the organisms was measured by [3h]uracil incorporation, and inhibition of multiplication was used as a measurement of immunity. although the immune lymphocytes restricted principally the multiplication of homologous organisms, partial protection, expres ...19873557619
[influence of the number of inoculated parasites on the in vitro infestation of hela cells by the coccidia besnoitia jellisoni frenkel].the influence of the number of bradyzoites of the coccidia besnoitia inoculated in the culture medium of hela cells on their penetration rate was investigated. the intracellular penetration rate increased with the "size of inoculum", then seemed constant when a certain density is reached. important densities of bradyzoites in the inoculum led to an unequal distribution of intracellular parasites among the cellular sheet. multiple infective units in a same host cell did not occur randomly. it see ...19863101572
immunohistochemical diagnosis of neospora caninum in tissue sections.an avidin-biotin-peroxidase complex immunoperoxidase staining method was developed to detect neospora caninum in formalin-fixed, paraffin-embedded tissue sections. specific antiserum to n caninum was made in rabbits and used to probe tissues from dogs naturally and experimentally infected with n caninum. the test detected tachyzoites and bradyzoites of n caninum. a reaction was not observed to toxoplasma gondii, hammondia hammondi, sarcocystis cruzi, s capricanis, s tenella, besnoitia jellisoni, ...19892694869
specificity of the carbon immunoassay (cia) test for the diagnosis of toxoplasma infections.a rapid and low cost procedure, the carbon immunoassay (cia) test, was evaluated for the diagnosis of toxoplasma gondii infections. using a closely related parasite (besnoitia jellisoni) as antigen, and homologous or heterologous immune sera, it was demonstrated by light and electron microscopy that cia is a very reliable and specific test. as it is neither expensive nor time-consuming, it can be recommended for general and routine laboratory use.19921466141
superinfection in mice previously infected with mycobacterium leprae.previous studies of the protection of mice by prior infection with mycobacterium leprae in one hind footpad against challenge with m.leprae in the opposite hind footpad had produced conflicting results; therefore, the problem was restudied. in several experiments, balb/c mice were inoculated first in the right hind footpad with 5,000 m. leprae and then challenged in the left hind footpad with 5,000 m. leprae of the same strain at intervals after primary infection, at the same time that uninfecte ...19751091561
specific antibody-dependent killing of toxoplasma gondii by normal macrophages.the requirement for specificity of antibody-dependent inhibition or killing of intracellular toxoplasma gondii trophozoites by normal mouse peritoneal macrophages was evaluated in vitro using light microscopy and autoradiography. anti-toxoplasma antibody in the presence of 'accessory factor' rendered extracellular t. gondii trophozoites non-viable and non-infectious for cells, whereas exposure of extracellular trophozoites to heat-inactivated immune serum did not appear to damage the parasites. ...19761009680
role of activated macrophages in resistance of mice to infection with trypanosoma cruzi.the role of the activated macrophage in resistance of mice to infection with trypanosoma cruzi was investigated with use of mice that had not been infected with t. cruzi but whose macrophages were activated by toxoplasma gondii or besnoitia jellisoni. mice with activated macrophages were significantly more resistant to intra-peritoneal challenge with the tulahuen strain of t. cruzi than were controls. the role of serum factors in resistance to t. cruzi was excluded by the demonstration of no dif ...1976826595
loss of stages after continuous passage of toxoplasma gondii and besnoitia jellisoni.toxoplasma gondii, passed from mouse to mouse in the tachyzoite stage for 30-35 generations, developed cysts, which when fed to cats, failed to produce oocysts. besnoitia jellisoni, passed similarly for 20 generations, lost the capacity to form cysts. these phenomena are explained by a loss of genomes or gene products during the rapid passage selecting for tachyzoites.1976823327
role of the micropore in nutrition of the sporozoa. ultrastructural study of plasmodium cathemerium, eimeria ferrisi, e. stiedai, besnoitia jellisoni, and frenkelia sp. 1976821187
mediation of immunity to intracellular infection (toxoplasma and besnoitia) within somatic cells.antigen-treated lymphocytes from immune hamsters specifically protected not only macrophages, but also cultured fibroblasts and kidney cells infected with toxoplasma gondii or besnoitia jellisoni. macrophages were not necessary for the protection of fibroblasts and kidney cells. a mediator that inhibited the intracellular proliferation of these microbes was obtained from immune lymphocytes in contact with specific antigen. again, macrophages were not necessary for the elaboration of this mediato ...1978640741
cellular immunity to toxoplasma and besnoitia in hamsters: specificity and the effects of cortisol.the inhibitory effects of cortisol on cellular immunity were studied in vitro by using hamster peritoneal exudate cells. two obligate, intracellular protozoa--toxoplasma gondii and besnoitia jellisoni-- were used to control for specificity of effects. results indicate that immune lymphocytes specifically confer immunity to (or "arm") macrophages that specifically express immunity. this arming can be inhibited by 5 microng of cortisol per ml. macrophages that have been armed already will continue ...1977404250
specific immunity and nonspecific resistance to infection: listeria, protozoa, and viruses in mice and hamsters,.specific immunity developed by mice against protozoan (toxoplasma gondii and besnoitia jellisoni) and bacterial (listeria monocytogenes) infections was compared with nonspecific protection conferred by prior infections. the results indicated that homologous immunity protected mice from more than 10-5 ld50 of t. gondii or b. jellisoni, but from only 10-2 ld50 of l. monocytogenes. heterospecific protection among these organisms was for 10-0.4 minus 10-1.2 ld50. in studies in hamsters specific immu ...1975165241
molecular analysis and characterization of a protein involved in the replication of intracellular toxoplasma gondii.previous studies in our laboratory have identified a cytoplasmic protein (p97) of t. gondii that is involved in the process of intracellular parasite replication. monoclonal antibody inhibits parasite replication in vitro and recognizes a protein of approximate 97 kda by western blot analysis. using biotinylation, we demonstrate that p97 is not expressed on the surface of the tachyzoite. polyclonal sera raised against the purified native protein was used to isolate a cdna of 3.3 kb from a librar ...19979476788
toxoplasma gondii: a monoclonal antibody that inhibits intracellular replication.during its intracellular life cycle within the infected host cell, toxoplasma gondii is able to undergo rapid asexual replication. neither the mechanism by which the parasite initiates this process nor the requirements for maintaining it are understood. we produced a monoclonal antibody, 1b8, that identifies a parasite antigen of approximate m(r) 97 kda as determined by sds-page. the epitope recognized by mab 1b8 appears as a collection of vesicular structures scattered throughout the cell cytop ...19947525337
specific mediation of cellular immunity to toxoplasma gondii in somatic cells of mice.lymphocytes from mice immunized against toxoplasma gondii protected t. gondii-infected macrophage and kidney cell cultures. after contact with antigens, supernatants of such immune lymphocytes, also contained a factor protective for t. gondii-infected macrophages and kidney cells. supernatants were protective only when the lymphocytes and kidneys cells were isogeneic. protection was specific in that supernatants from only t. gondii-immune, but not besnoitia jellisoni-immune, lymphocytes provided ...19846500716
serodiagnosis of bovine besnoitiosis by elisa and immunofluorescence tests.sera from non-infected cattle and cattle infected with anaplasma, babesia, theileria and sarcocystis were tested for antibodies to besnoitia in elisa and immunofluorescence tests (ift) with besnoitia besnoiti of blue wildebeest origin as antigen. only 2 out of 86 sera gave false positive reactions in elisa and none in the ift, indicating a high specificity for the tests. three-hundred-and-three bovine sera from 3 farms in an area endemic for besnoitiosis were similarly tested and the results wer ...19846442765
resistance of akr mice to lymphoid leukemia associated with a chronic protozoan infection, besnoitia jellisoni. 19715559245
besnoitia jellisoni (sporozoa: toxoplasmea) in rodents from utah and california. 19684990449
molecular phylogeny of besnoitia and the genetic relationships among besnoitia of cattle, wildebeest and goats.knowledge on parasites of the genus besnoitia is sparse, which are classified in the subfamily toxoplasmatinae of the phylum apicomplexa. this arrangement hypotheses that besnoitia represents the sister group to species such as toxoplasma gondii and hammondia hammondi. in order to test this hypothesis, phylogenetic analyses of 18s ribosomal dna (rdna) from besnoitia, hammondia, isospora, frenkelia, eimeria, neospora, sarcocystis and toxoplasma were performed. the 18s rdna of besnoitia besnoiti, ...200011212893
effects of hormones on the adrenal necrosis produced by besnoitia jellisoni in golden hamsters.adrenal necrosis has been described in golden hamsters where it occurs during the course of infection with besnoilia jellisoni. this necrosis results directly from the active intracellular proliferation by this obligate intracellular protozoan organism. after infection, adrenal necrosis is rarely observed in hypophysectomized hamsters. in unoperated animals adrenal necrosis is suppressed to varying degrees by cortisone (e), hydrocortisone (f), corticosterone (b), 11-dehydrocorticosterone (a), an ...195613295493
similar internal morphology of toxoplasma gondii and besnoitia jellisoni stained with silver protein. 195713463701
antigenic relationship of toxoplasma gondii and besnoitia jellisoni. 196514275222
resistance to mycobacterium leprae in mice infected with toxoplasma gondii and besnoitia jellisoni.mice chronically infected with the intracellular protozoan toxoplasma gondii or besnoitia jellisoni were resistant to footpad challenge with mycobacterium leprae. resistance was manifested by lower numbers of recoverable m. leprae in the footpads of protozoal-infected mice and was enhanced in toxoplasma-infected mice by a booster injection of toxoplasma antigen in the infected footpad. the results suggest a major role for the activated macrophage in the control of m. leprae infection.197416558091
molecular biological comparison of different besnoitia species and stages from different countries.besnoitia besnoiti tissue cysts from a recent outbreak in cattle in germany were characterized with respect to their internal transcribed spacer regions 1, 2, and 18s rdna gene sequences. these results were compared with own sequences of an israelian isolate of b. besnoiti and of besnoitia jellisoni cystozoites stored for years in liquid nitrogen. furthermore, material was studied that was obtained from white mice (balb/c) that had been successfully infected by intraperitoneal infection of fresh ...201020135147
resistance to murine tumors conferred by chronic infection with intracellular protozoa, toxoplasma gondii and besnoitia jellisoni. 19715127071
a comparison of cross protection between bcg, hammondia hammondi, besnoitia jellisoni and toxoplasma gondii in hamsters.the effect of pretreatment with bcg strain of mycobacterium tuberculosis or hammondia hammondi 21 days before challenge with lethal doses of t oxoplasma gondii and besnoitia jellisoni was studied in hamsters. the results indicated that the intracardial administration of bcg provided no protection against either t. gondii or b. jellisoni. the hamsters immunized with h. hammondi survived challenge with 10(4) lethal doses of t. gondii but only 1 lethal dose with b. jellisoni, indicating strong cros ...1978364045
[developmental possibilities of besnoitia jellisoni (toxoplasmatea protozoan parasite) in tissue culture. some aspects of its behabior in chick embryo fibroblasts]. 19684236233
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