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phenotypic, serological and genetic characterization of pseudomonas anguilliseptica strains isolated from cod, gadus morhua l., in northern europe.the biochemical, serological and genetic characteristics of six strains of pseudomonas anguilliseptica isolated from cod, gadus morhua, in scotland were compared to well characterized isolates of this same bacterial species but of different origin. biochemical and physiological analyses showed that this group of isolates was highly homogeneous, their characteristics matching previous descriptions of the pathogen. similar results were obtained for the six cod isolates in the serological assays, a ...200717958609
Characterisation of histamine-producing bacteria from farmed blackspot seabream (Pagellus bogaraveo) and turbot (Psetta maxima).Turbot (Psetta maxima) and blackspot seabream (Pagellus bogaraveo) represent two of the most important emerging farmed fish species in European countries. However, no information of the presence and development of histamine-producing bacteria on them has been reported so far. Accordingly, the aim of this study was to isolate and identify the main histamine-producing bacteria in farmed turbot and blackspot seabream. For this study, 24 isolates (12 from turbot and 12 from blackspot seabream) were ...201121925757
discord in the family sparidae (teleostei): divergent phylogeographical patterns across the atlantic-mediterranean divide.the strait of gibraltar has been proposed to be the divide between two marine biogeographical regions, the mediterranean sea and the northeast atlantic. intraspecific studies have shown, for several of the examined species, a reduction of gene flow between the two basins. the present study examines genetic variation at nuclear and mitochondrial loci in five marine teleost species belonging to the family sparidae. four samples for each species were analysed spanning the northeast atlantic and the ...200314640406
olfactory sensitivity to amino acids in the blackspot sea bream (pagellus bogaraveo): a comparison between olfactory receptor recording techniques in seawater.the current study investigated the olfactory sensitivity of the blackspot sea bream to amino acids, odorants associated with food detection in fish, and compared the efficacy of two different experimental methods: multi-unit recording from the olfactory nerve and the electro-olfactogram (eog). twenty essential amino acids plus l-dopa evoked clear, concentration-dependent olfactory responses using both methods, with estimated thresholds of 10(-8.5)-10(-6.2) m (nerve recording) and 10(-7.5)-10(-4. ...201121544618
response to short term starvation of growth, haematological, biochemical and non-specific immune parameters in european sea bass (dicentrarchus labrax) and blackspot sea bream (pagellus bogaraveo).growth, haematological (haematocrit), biochemical (serum cortisol and glucose), and non-specific immune (lysozyme, serum haemolytic and haemagglutinating activities, extracellular respiratory burst activity) parameters, were monitored in european sea bass dicentrarchus labrax and blackspot sea bream pagellus bogaraveo subjected to a 31 days starvation compared to fed fish, to assess the responses to feed deprivation of these health status indicators. while haematocrit, serum cortisol, glucose an ...201121664688
the effect of elevated hydrostatic pressure upon selected biomarkers in juvenile blackspot seabream pagellus bogaraveo in a 14 day-long experiment.hydrostatic pressure elevated to 500 kpa for 14 days was found to affect hepatic 7-ethoxyresorufin-o-deethylase (erod), oxidized protein (pox), protein yield and branchial na(+)-k(+)-atpase. no effect on glutathione-s-transferase (gst), superoxidase dismutase (sod), catalase (cat), lipid peroxidation (lp), acetylcholinesterase (ache), butyrylcholinesterase (bche), condition factor (k) and hepato-somatic index (i(h)) was encountered.201020646152
polymerase chain reaction amplification of repetitive intergenic consensus and repetitive extragenic palindromic sequences for molecular typing of pseudomonas anguilliseptica and aeromonas salmonicida.the aim of this study was to evaluate the use of two molecular techniques, repetitive extragenic palindromic polymerase chain reaction (rep-pcr) and repetitive intergenic consensus pcr (eric-pcr), as epidemiological tools with which to discriminate among genetically distinct strains within two bacterial fish pathogens, pseudomonas anguilliseptica and aeromonas salmonicida. a total of 30 a. salmonicida and 52 p. anguilliseptica were analyzed. for p. anguilliseptica, three different major fingerpr ...200818783128
fine structure of spermatozoa in the blackspot sea bream pagellus bogaraveo (brünnich, 1768) with some considerations about the centriolar complex.scanning and transmission electron microscopy were used to investigate the fine structure of the sperm of the sparid fish pagellus bogaraveo. the spermatozoon of p. bogaraveo belongs, like that of the other sparid fish, to the teleostean "type i" spermatozoon with the flagellar axis insert perpendicular to the nuclear fossa. it has an ovoidal head, a short, cylindrically shaped midpiece and a long tail region. the nucleus reveals a deep invagination (nuclear fossa), in which the centriolar compl ...201020060993
comparing deep-sea fish fauna between coral and non-coral "megahabitats" in the santa maria di leuca cold-water coral province (mediterranean sea).two experimental longline surveys were carried out in the santa maria di leuca (sml) cold-water coral province (mediterranean sea) during may-june and september-october 2010 to investigate the effect of corals on fish assemblages. two types of "megahabitat" characterized by the virtual absence of fishing were explored. one was characterized by complex topography including mesohabitats with carbonate mounds and corals. the other type of megahabitat, although characterized by complex topographic f ...201223028551
Infection levels and diversity of anisakid nematodes in blackspot seabream, Pagellus bogaraveo, from Portuguese waters.The blackspot seabream, Pagellus bogaraveo, is a sparid fish of great economic importance in the northeast Atlantic. The main aim of this work was to assess the infection levels and diversity of anisakid nematodes parasitizing P. bogaraveo from Portuguese waters. The anisakid larvae were identified by polymerase chain reaction-restriction fragment length polymorphism analysis and ten different patterns were observed, four of which were not previously reported in the literature. Moreover, several ...201122139402
isolation of lymphocystis disease virus from sole, solea senegalensis kaup, and blackspot sea bream, pagellus bogaraveo (brunnich).two viruses were isolated from cultured sole, solea senegalensis, and wild blackspot sea bream, pagellus bogaraveo, and preliminarily characterized as lymphocystis disease viruses (lcdvs). viral isolates were characterized by morphological, biochemical and biophysical properties. in addition, the susceptibility of four fish cell lines was also tested. lcdv isolates developed cytopathic effects on the saf-1 cell line at 5 and 6 days post-infection and reached titres of 10(6) tcid50 ml(-1). the an ...200515813864
protein and glycoprotein content of lymphocystis disease virus (lcdv).the polypeptide and glycoprotein composition of eight strains of the fish-pathogenic lymphocystis disease virus (lcdv) isolated from gilt-head seabream (sparus aurata), blackspot seabream (pagellus bogaraveo), and sole (solea senegalensis) were determined. the protein electrophoretic patterns of all lcdv isolates were quite similar regardless of the host fish, showing two major proteins (79.9 and 55.6 kda) and a variable number of minor proteins. three groups of lcdv isolates were distinguished ...200415248160
parasite communities of adriatic cage-reared fish.from june 2001 to march 2002, 7 semi-offshore facilities in the adriatic sea rearing sea bass dicentrarchus labrax, sea bream sparus aurata, sharpsnout bream diplodus puntazzo and red sea bream pagellus bogaraveo aged 1+, were monitored for the presence of protozoan and metazoan parasites. obtained data sets of abundance and prevalence were used to evaluate the structure and dynamic of infra- and component parasite communities. in all hosts, except the red sea bream, dominant parasites were mono ...200515900691
gastrointestinal helminth communities of the blackspot seabream pagellus bogaraveo (teleostei: sparidae) from portuguese north-east atlantic waters.a study was carried out on the gastrointestinal helminth communities of blackspot seabream, pagellus bogaraveo, from the portuguese mainland and island waters. fifteen helminth parasite species were detected, including the following new records in this host: the digeneans accacladocoelium petasiporum, brachyenteron helicoleni, glomericirrus macrouri, lecithocladium excisum, lepocreadium album and pachycreadium carnosum; the nematode camallanus sp. and the acanthocephalan rhadinorhynchus pristis. ...201423182041
differentiation of sparidae species by dna sequence analysis, pcr-sscp and ief of sarcoplasmic proteins.the increasing global trade of fishery and aquaculture products makes it necessary to develop methods for species identification in case of fish fillets or other highly processed seafood with external morphological characteristics (e.g. gills, fins) of the original fish being removed. species identification methods based on dna- or protein-analysis, like dna sequencing of pcr products, pcr-sscp (polymerase chain reaction - single strand conformation polymorphism), and isoelectric focusing (ief) ...201323265470
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