| the mating system of the true fruit fly bactrocera tryoni and its sister-species, bactrocera neohumeralis. | the frugivorous 'true' fruit fly, bactrocera tryoni (queensland fruit fly), is presumed to have a non-resourced-based lek mating system. this is largely untested, and contrary data exists to suggest bactrocera tryoni may have a resource-based mating system focused on fruiting host plants. we tested the mating system of bactrocera tryoni, and its close sibling bactrocera neohumeralis, in large field cages using laboratory reared flies. we used observational experiments that allowed us to determin ... | 2016 | 27006172 |
| wolbachia pseudogenes and low prevalence infections in tropical but not temperate australian tephritid fruit flies: manifestations of lateral gene transfer and endosymbiont spillover? | maternally inherited wolbachia bacteria infect many insect species. they can also be transferred horizontally into uninfected host lineages. a wolbachia spillover from an infected source population must occur prior to the establishment of heritable infections, but this spillover may be transient. in a previous study of tephritid fruit fly species of tropical australia we detected a high incidence of identical wolbachia strains in several species as well as wolbachia pseudogenes. here, we have in ... | 2015 | 26385192 |
| the microbiome of field-caught and laboratory-adapted australian tephritid fruit fly species with different host plant use and specialisation. | tephritid fruit fly species display a diversity of host plant specialisation on a scale from monophagy to polyphagy. furthermore, while some species prefer ripening fruit, a few are restricted to damaged or rotting fruit. such a diversity of host plant use may be reflected in the microbial symbiont diversity of tephritids and their grade of dependency on their microbiomes. here, we investigated the microbiome of six tephritid species from three genera, including species that are polyphagous pest ... | 2015 | 25666536 |
| tropical tephritid fruit fly community with high incidence of shared wolbachia strains as platform for horizontal transmission of endosymbionts. | wolbachia are endosymbiotic bacteria that infect 40-65% of arthropod species. they are primarily maternally inherited with occasional horizontal transmission for which limited direct ecological evidence exists. we detected wolbachia in 8 out of 24 australian tephritid species. here, we have used multilocus sequence typing (mlst) to further characterize these wolbachia strains, plus a novel quantitative polymerase chain reaction method for allele assignment in multiple infections. based on five m ... | 2014 | 24428638 |
| the cryptochrome (cry) gene and a mating isolation mechanism in tephritid fruit flies. | two sibling species of tephritid fruit fly, bactrocera tryoni and bactrocera neohumeralis, are differentiated by their time of mating, which is genetically determined and requires interactions between the endogenous circadian clock and light intensity. the cryptochrome (cry) gene, a light-sensitive component of the circadian clock, was isolated in the two bactrocera species. the putative amino acid sequence is identical in the two species. in the brain, in situ hybridization showed that cry is e ... | 2004 | 15611172 |
| the effects of selection for early (day) and late (dusk) mating lines of hybrids of bactrocera tryoni and bactrocera neohumeralis. | bactrocera neohumeralis and bactrocera tryoni are closely related tephritid fruit fly species. b. neohumeralis mates throughout the day (in bright light) and b. tryoni mates at dusk. the two species can also be distinguished by the colour of their calli (prothoracic sclerites) which are brown and yellow, respectively. the f1 hybrids can mate both in bright light just before dusk and during dusk and have calli that are partly brown and partly yellow. the f2 hybrids have a wider range of callus pa ... | 2003 | 14686607 |
| the period gene in two species of tephritid fruit fly differentiated by mating behaviour. | the period gene is important for the generation and maintenance of biological rhythms. it served as an ideal candidate for the investigation of the mating time isolation between two sibling queensland fruit fly species, bactrocera tryoni and bactrocera neohumeralis. we have isolated the homologues of the period gene in the two species, and show that their putative amino acid sequences are identical. no length polymorphism was detected in the thr-gly repeat region. per mrna expression, assayed in ... | 2002 | 12230541 |
| the genome of the queensland fruit fly bactrocera tryoni contains multiple representatives of the mariner family of transposable elements. | representatives of five distinct types of transposable elements of the mariner family were detected in the genomes of the queensland fruit fly bactrocera tryoni and its sibling species bactrocera neohumeralis by phylogenetic analysis of transposase gene fragments. three mariner types were also found in an additional tephritid, bactrocera jarvisi. using genomic library screening and inverse pcr, full-length elements representing the mellifera subfamily (b. tryoni.mar1) and the irritans subfamily ... | 2001 | 11520360 |
| australian endemic pest tephritids: genetic, molecular and microbial tools for improved sterile insect technique. | among australian endemic tephritid fruit flies, the sibling species bactrocera tryoni and bactrocera neohumeralis have been serious horticultural pests since the introduction of horticulture in the nineteenth century. more recently, bactrocera jarvisi has also been declared a pest in northern australia. after several decades of genetic research there is now a range of classical and molecular genetic tools that can be used to develop improved sterile insect technique (sit) strains for control of ... | 2014 | 25470996 |
| multiple subfamilies of mariner transposable elements are present in stalk-eyed flies (diptera: diopsidae). | the diopsid stalk-eyed flies are an increasingly well-studied group. presented here is evidence of the first known transposable elements discovered in these flies. the vertumnana mariner subfamily was identified in the diopsini tribe, but could not be amplified in species of the sphyracephalini tribe. pcr screening with degenerate primers revealed that multiple mariner subfamilies are present within the diopsidae. most of the sequenced elements appear to be pseudogenes; however two subfamilies a ... | 2008 | 17562187 |
| the likely fate of hybrids of bactrocera tryoni and bactrocera neohumeralis. | bactrocera tryoni (froggatt) and b. neohumeralis (hardy) (diptera: tephritidae) are sympatric species which hybridise readily in the laboratory yet remain distinct in the field. b. tryoni mates only at dusk and b. neohumeralis mates only during the day, but hybrids can mate at both times. we investigated the inheritance of mating time in successively backcrossed hybrid stocks to establish whether mating with either species is more likely. the progeny of all backcrosses to b. tryoni mated only at ... | 2003 | 12714981 |