| survival of salmonellae during pepperoni manufacture. | survival of salmonellae in artificially contaminated beef-pork mixtures (approximately 10(4) salmonellae/g) was studied in pepperoni prepared by either a natural flora or lactic starter culture fermentation or in nonfermented sausages. the pepperoni did not become salmonellae free during the usual commercial 15 to 30-day drying period. salmonella dublin was present in all products, fermented or unfermented, after 42 to 43 days of drying. at a lower level of contamination, 10(3)/g, s. dublin coul ... | 1975 | 951 |
| effects of intravenous salmonella dublin on cattle at different stages of fasciola hepatica infection. | | 1978 | 27541 |
| immunological responses of fluke-infected and fluke-free cattle to salmonella dublin and other antigens. | immune responses to heat-killed brucella abortus strain 19 and to ovalbumin were compared in 15 fluke-infected and 15 fluke-free friesian heifers. b abortus was injected 16 weeks and ovalbumin 19 weeks after the oral administration of 1000 metacercariae of fasciola hepatica. agglutinating antibody responses to b abortus were similar in both groups. immediate type hypersensitivity to ovalbumin was apparently suppressed in fluke-infected animals when assessed by active and passive cutaneous anaphy ... | 1979 | 120572 |
| the in vitro drug resistance of salmonella dublin isolated from cattle. | the resistance of salmonella dublin to eight antibacterial drugs was assessed under diagnostic laboratory conditions. two techniques were used consecutively and the differences in the results are described. strains of s. dublin isolated from either calves, adult cattle or abortion material showed no differences in resistance for either of the techniques, though differences between the two techniques were found. | 1977 | 143494 |
| [stress and immunity in cattle]. | stress of variable length may be caused in calf by parenteral administration of supercortisol and application of acute thermal load. hormonal or thermal stress may have negative impact on postvaccinal immunity to paratyphoid (salmonella dublin 81), as may be interpreted with reference to the o-agglutination titre of blood serum. the extent to which immunity is suppressed by stress was found to depend on the moment of stress application, duration of stress, and age of the calf. | 1979 | 222226 |
| [salmonella osteomyelitis (authors transl)]. | a case of osteomyelitis due to salmonella dublin is reported by the authors who underline the features which distinguish this affection from typhous osteomyelitis, which is completely different. | 1979 | 228429 |
| salmonella dublin infection in adult cattle: a slaughter house and knackery survey in south west wales. | a survey was carried out between june 1970 and december 1971 in which gall bladders from cattle either slaughtered for food consumption or disposed of through knackeries were cultured for salmonellas. salmonella dublin was isolated from 28 of 1917 gall-bladders of adult cattle killed in the slaughterhouse at carmarthen, and from 23 of 197 gall-bladders obtained from adult cattle sent to a knackery at abergwili, near carmarthen. the results are compared with a similar survey carried out in 1947 a ... | 1977 | 264496 |
| choice of selective media for the isolation of salmonella dublin from experimentally contaminated bovine faeces. | | 1978 | 288115 |
| the diagnosis of salmonella abortion in cattle with particular reference to salmonella dublin. a review. | the diagnosis of abortion in cattle caused by salmonella dublin depends upon the isolation of the organism from either the products of conception, uterine discharges, vaginal mucus or milk together with serological evidence of active infection. s. dublin may be isolated when an active or a latent carrier cow abourts but in these cases an active infection will not be demonstrable. the retrospective identification of a case of s. dublin abortion may prove difficult as excretion of the organism is ... | 1977 | 328768 |
| acquisition of multiple antibiotic resistance by salmonella dublin from the gramnegative hospital flora, in a kidney allograft recipient. | the case of a kidney allograft recipient, who suffered from several episodes of salmonella dublin sepsis following massive immunosuppressive therapy to overcome a transplant rejection crisis, is presented. the focus of sepsis was the chronic inflamed gallbladder. the salmonella dublin strain isolated from the blood during the last episode was found to exhibit multiple resistance to antimicrobiol drugs. because the resistance phenotype was characteristic for the gramnegative flora of the universi ... | 1978 | 364885 |
| studies of the virulence of salmonella dublin in experimental infections of cattle and rats. | | 1979 | 435961 |
| association between raw milk and human salmonella dublin infection. | between 1971 and 1975 the mean annual incidence of human salmonella dublin infection in california increased more than five-fold. investigation of the increase showed an association with exposure to raw milk in 44 out of 113 cases. of these 44 patients, 35 had used certified raw milk from a single dairy. faecal swabs confirmed s dublin infection in the dairy herd and the milk, and so a pasteurisation order was issued. s dublin appears to be an unusually invasive and life-threatening salmonella s ... | 1979 | 476402 |
| immunoglobulins detected by the serum agglutination test for salmonella dublin infection of cattle. | | 1979 | 484132 |
| association between raw milk and human salmonella dublin infection. | | 1979 | 497741 |
| plasmid-mediated antimicrobial drug resistance in salmonella dublin in food animals. | | 1979 | 552708 |
| a study of the pathogenesis of experimental salmonella dublin abortion in cattle. | | 1977 | 557060 |
| epidemiology of salmonella infection in calves: the source of calfhood infection by salmonella dublin. | investigation of the source of neonatal salmonella dublin infection of calves was undertaken by carrying out caesarean section of cows with a history of excretion of s dublin following either s dublin enteritis or s dublin abortion. no evidence of transplacental infection was detected but six of 10 animals showed evidence of excretion of the organism in the faeces, vaginal discharge or milk in the period immediately following parturition. the strong probability of early infection as a result of ... | 1977 | 564570 |
| a milk-borne outbreak due to salmonella dublin. | salmonella dublin is primarily adapted to bovines and is a relatively rare cause of human illness. an outbreak is described in which it was estimated that at least 700 persons were infected from milk which had not been subjected to heat treatment. although the organism was isolated from retail samples of milk, investigations at the dairy farm were inconclusive and a number of questions are posed. attention is drawn to the value of inter-disciplinary cooperation in the investigation of the outbre ... | 1979 | 570201 |
| salmonella dublin in dairy cattle. | | 1977 | 588184 |
| experimental salmonella dublin infection in calves. | | 1977 | 608079 |
| diagnosis of bovine salmonellosis ii. application of the indirect haemagglutination test to the diagnosis of salmonella dublin infection. | | 1977 | 608097 |
| effects of experimental salmonella dublin infection in cattle given fasciola hepatica thirteen weeks previously. | | 1978 | 621306 |
| chronic salmonella dublin infection in calves. | | 1978 | 630373 |
| intrauterine infection of calves with salmonella dublin. | | 1978 | 644818 |
| the pathogenesis of experimental intra-ruminal infections of cows with salmonella dublin. | | 1978 | 670441 |
| investigation of a cutaneous delayed hypersensitivity response as a means of detecting salmonella dublin infection in cattle. | delayed hypersensitivity reactions developed 48 to 96 h after intradermal injection of killed salmonella dublin in 25 of 28 cattle which had been inoculated intravenously, and in five of 10 cattle which had been inoculated orally with s dublin 24 to 493 days previously. control animals showed no delayed hypersensitivity reactions. persistence of infection in five of the intravenously inoculated and in four of the orally inoculated animals was confirmed by isolation of s dublin from the carcases ... | 1978 | 674850 |
| the serology of experimental salmonella dublin infections of cattle. | agglutinating antibodies to somatic "o" and flagellar "h" antigens of s. dublin were measured in the serum of 43 pregnant heifers before intravenous or oral infection with s. dublin and in the serum of 21 uninfected control animals. the data from these animals were analysed statistically and a titre of 1/80, to both antigens, has been interpreted as of doubtful significance and a titre of 1/160 to both antigens, has been interpreted as significantly raised. animals in which fetal infection occur ... | 1978 | 690423 |
| experimental intraperitoneal salmonella dublin infection in rats: effects of concurrent infections with fasciola hepatica and nippostrongylus brasiliensis. | | 1978 | 711936 |
| treatment of experimental calf salmonellosis with amoxycillin. | treatment of experimental salmonella dublin infection in the calf with amoxycillin is described. in most animals a rapid response occurred when the drug was administered by the parenteral route. results were moderately good when the oral route was used in calves fed solely on a milk replacer diet. in calves consuming hay and concentrates oral administration was much less efficient. | 1978 | 716167 |
| experimental oral infections of pregnant heifers with salmonella dublin. | | 1979 | 761062 |
| [biochemical identification of salmonella and coli bacteria and their sensitivity to chemotherapeutic agents]. | strains of enterobacteria, isolated from pathologic material (dead pigs, birds, lambs, sheep fetuses, swab fecal samples from calves) were biochemically identified by the methods of costin, le minor, and eming. one-hundred forty of them proved to belong to escherichia coli and 236 -- to salmonella. the sensitivity of the isolated bacteria to antibiotics (ampicillin, streptomycin, tetracyclin, kanamycin, neomycin, chloramphenicol, erythromycin, oleandomycin polymyscin), sulfonamides (norsulfasol, ... | 1976 | 785798 |
| the effect of the microflora in cattle slurry on the survival of salmonella dublin. | | 1977 | 837200 |
| [problems in the use of radioactively marked bacteria in animal experiments. 1. labeling of pasteurella multocida, pasteurella haemolytica and salmonella dublin with eh, 14c, 32p, 59fe, 99mtc, 125j1]. | several methods are suggested by which to use the radionuclides 3h, 14c, 32p, 59fe, 99mtc, and 125j for labelling or doublelabelling of pasteurella multocida, pasteurella haemolytica, and salmonella dublin, with particular reference being made to labelling ofr animal experiments. suitable radioactive substrates for internal labelling in chemically defined or partially defined nutritive media include 3h-thymin, 3h-thymidine, 14c-glucose, 14c-mannose, 14c-aspartic acid, as well as 3h-uracil, 3h-ur ... | 1977 | 849104 |
| [studies on calf salmonellosis. 3. preclinical evaluation of a smd. salmonella dublin live vaccine]. | results of preclinical assay, preparation and conservation of a s.-dublin live vaccine based on streptomycin dependent mutants and for oral application are demonstrated and discussed in detail. the used oral vaccine is very good tolerated by calves and results in stable immunity after administration of a daily dose of 5 x 10(10) bis 1 x 10(11) living smd.-mutants for 10 consecutive days. complete immunity developes in calves within a period of two weeks after the last antigen administration and ... | 1977 | 849107 |
| [studies on salmonellosis in the calf. 5. clinical testing of smd-salmonella-dublin vaccine for oral administration]. | the oral vaccine, prepared on the basis of streptomycindependent mutants for administration to control s.-dublin infection, was clinically tested on 2,210 calves of 22 herds. the experimentally obtained findings regarding good tolerance and sufficient effectiveness of oral live vaccines were thus confirmed under field conditions. good effectiveness of the oral vaccine was reflected in the recorded decline of clinical salmonellosis cases and of calf losses due to salmonellosis, and it was establi ... | 1977 | 901130 |
| experimental salmonella dublin infection in calves. | an experimental model of salmonella dublin infection in calves for the purpose of evaluating antibacterial compounds was developed. oral administration of the bacterium produced variable results and subsequently the organism was injected intravenously into one-week-old calves. symptoms observed were consistent with those described in naturally occurring cases. an analysis of the mortality and clinical signs in both control and treated calves showed that the severity of the disease was exacerbate ... | 1977 | 919266 |
| salmonella dublin meningitis. report of a case. | | 1977 | 924608 |
| an experimental study of salmonella dublin abortion in cattle. | | 1976 | 944070 |
| [mass food poisoning caused by salmonella dublin]. | | 1976 | 945593 |
| a study of the complement fixation test in salmonella dublin infection. | the complement fixation test was evaluated in a study of salmonella dublin infection in cattle. in calves vaccinated with a live s dublin vaccine at three days of age, complement fixing (cf) antibodies were not detected in colostrum-fed calves although cf antibodies were detected at low titre in colostrum-deprived calves. in calves vaccinated at three months of age and in experimentally infected cows, cf antibodies persisted for three to six months but serum agglutinin (sa) antibodies persisted ... | 1976 | 968186 |
| [culture media for salmonella dublin on a chemically defined basis]. | the nutrient demand of s-dublin strains was investigated. media on chemically defined basis for culturing of s. dublin are recommended with reference to the above studies. the definition includes na2hpo4, kh2po4, nacl, glucose, the amino acids l-asparaginic acid, l-cystine, l-glutamic acid, serine, and l-threonine, and nicotinamide as vitamin component. such media will be quite favourable for certain culturing problems, but complex substrate (yeast extract, peptone, tryptone) should be added for ... | 1976 | 985017 |
| immunization of mice and calves against salmonella dublin with attenuated live and inactivated vaccines. | previous findings, viz. that mice can be successfully immunized against infection with salmonella dublin with either live or inactivated vaccine, were confirmed. immunity lasted for at least 12 weeks in mice which had been immunized with inactivated alum-precipitated vaccine. the immunogenicity of inactivated vaccine gradually decreased on storage at 4 degrees c, but this was only detectable if a single injection was used for immunization: 2 injections virtually eliminated this phenomenon. the i ... | 1976 | 1018887 |
| the effect of storage in slurry on the virulence of salmonella dublin. | the mouse was used as a model to determine whether storage of salmonella dublin in slurry and in broth reduces the virulence of the organism. no reduction in virulence of s. dublin stored in slurry for 36 days or in maintenance broth for 70 days was observed. the disease hazard involved in pasture-spreading of slurry contaminated with salmonellas is related to factors other than virulence. | 1975 | 1054060 |
| studies on the isolation of salmonella dublin. | abattoir drain swabs, bovine faeces and a few other veterinary samples were examined for the presence of salmonella dublin. three selective agar media and four enrichment broths were investigated. the two most efficient plating media were deoxycholate citrate agar and brilliant green macconkey agar. wilson and blair's bismuth sulphite agar (de loureiro's modifications) was least successful. selenite f broth, whether incubated at 37 or 43 degrees c., was better than the other enrichment broths us ... | 1975 | 1056962 |
| a comparison of salmonella dublin infection in normal and endotoxin-tolerant mice. | | 1975 | 1095617 |
| immunization of mice and guinea-pigs against salmonella dublin infection with live and inactivated vaccine. | the immunogenicity of a number of avirulent rough salmonella dublin mutants was compared in mice and guinea-pigs. live vaccine prepared from strain hb 1/17 at doses of 5 x 10(7) per mouse usually gave an immunity of between 70 and 80% but in certain experiments the results were more variable and always poorer. this strain gave a cross protection of 28,5% to s. typhimurium in mice. in guinea-pigs it evoked an average protection of approximately 46% to homologous challenge and approximately 26% to ... | 1975 | 1107926 |
| salmonella dublin abortion in cattle: incidence and epidemiology. | | 1975 | 1125763 |
| differentiation of rough field strains of salmonella dublin from the part-rough vaccine strain hws51. | | 1975 | 1127149 |
| salmonella dublin septicemia in a scottish terrier recently imported from england. | | 1975 | 1131796 |
| a comparative study of liver changes produced by inoculating pregnant rats with salmonella dublin or with its endotoxin. | rats were inoculated with viable salmonella cublin organisms or a crude salm. dublin endotoxin at the 14th day of pregnancy. they were killed at intervals up to 96 h after inoculation and the pathogenesis of the liver lesions compared. the immediate effects on the liver of both inoculations were the same but there were differences in the later stages. in the endotoxin inoculated rats the initial liver lesions regressed but in the bacteria inoculated rats fat accumulated in the periportal and mid ... | 1975 | 1191517 |
| a comparison of indirect hemagglutination tests and serum agglutination tests for the serological diagnosis of salmonella dublin infection in cattle. | | 1975 | 1212611 |
| the effect of fascioliasis on susceptibility of cattle to salmonella dublin. | | 1976 | 1260411 |
| [salmonella dublin on dairy farms in the north of the netherlands]. | during the last century many calves and cows with salmonella dublin infections are diagnosed in the north of the netherlands, especially in the province of friesland. in this paper are retrospectively traced the problems by paratyphoid infections in cattle, from 1919 till now, the diagnosis of which was made at the animal health service in friesland and north-netherlands. over the decennia a decrease is noticed for the percentage of necropsies of calves with s. dublin infections, although some i ... | 1992 | 1287898 |
| cloning and nucleotide sequence of the gene (citc) encoding a citrate carrier from several salmonella serovars. | the nucleotide sequence of the citc coding for the citrate carrier in several salmonella serovars has been determined, and the amino acid sequence of the carrier protein was deduced. the predicted citrate carrier from salmonella pullorum and salmonella enteritidis consists of 446 amino acids with a molecular weight of 47,621, whereas that from salmonella dublin is the same 446 amino acids with the slightly different molecular weight of 47,591, because 1 amino acid residue was substituted. the pr ... | 1992 | 1374406 |
| synthetic recombinant vaccine expressing influenza haemagglutinin epitope in salmonella flagellin leads to partial protection in mice. | the influenza virus haemagglutinin epitope 91-108, which is a conserved amino acid sequence in all type a h3 strains, was expressed in salmonella flagellin, to evaluate its potential as a vaccine. for that purpose, a synthetic oligonucleotide comprising 54 bases coding for the corresponding sequence was inserted into the plasmid pls408 and transformed into escherichia coli jm101. colonies containing the recombinant plasmid were used to transform salmonella typhimurium lb5000 and were then transd ... | 1992 | 1376012 |
| regulation of plasmid virulence gene expression in salmonella dublin involves an unusual operon structure. | the 80-kb plasmid psdl2 of salmonella dublin lane is essential for lethal systemic infection in experimental mice. a cluster of five plasmid genes, designated spvr, spva, spvb, spvc, and spvd, is sufficient to express the plasmid-related virulent phenotype. the spvr gene product has recently been identified as a positive regulator of spvb expression in the stationary phase of bacterial growth (f. c. fang, m. krause, c. roudier, j. fierer, and d. g. guiney, j. bacteriol. 173:6783-6789, 1991). in ... | 1992 | 1378053 |
| characterization of translation termination mutations in the spv operon of the salmonella virulence plasmid psdl2. | the spv region of the salmonella virulence plasmids consists of five genes located on an 8-kb fragment previously shown to be essential for virulence in mice. four structural genes, spvabcd, form an operon that is transcriptionally activated by the spvr gene product in the stationary phase of growth. the role of the individual spv genes in the virulence phenotype was tested by isolating translation termination linker insertions in each gene. analysis of proteins synthesized in minicells identifi ... | 1992 | 1400193 |
| production of an electrolyte beverage from milk permeate. | the objective of this study was to prepare shelf-stable electrolyte beverages from milk permeate. the average composition of permeate was 4.59% total solids and .40% ash. lactose was hydrolyzed (approximately 80%) with a commercial fungal lactase enzyme. additional sweetness was provided by sucrose. the ph was reduced to 3.5 to 3.8 by the addition of citric acid. shelf-stable products were made using four processes: 1) uht followed by aseptic filling, 2) heating of filled bottles to 85 degrees c ... | 1992 | 1452842 |
| effect of calf age and salmonella bacterin type on ability to produce immunoglobulins directed against salmonella whole cells or lipopolysaccharide. | a commercially available salmonella bacterin was administered to holstein calves starting at 1 to 19 weeks of age. serum samples were obtained before administering bacterin and at 2-week intervals thereafter. an elisa with salmonella dublin lipopolysaccharide (lps) or s dublin whole cells as antigen, was used to measure specific igg and igm responses. antibody responses to lps were not detected from calves < 12 weeks old inoculated with killed bacterin. immunoglobulin responses to whole-cell ant ... | 1992 | 1456538 |
| an outbreak of salmonella dublin infection in england and wales associated with a soft unpasteurized cows' milk cheese. | an outbreak of salmonella dublin infection occurred in england and wales in october to december 1989. forty-two people were affected, mainly adults, and most lived in south-east england. microbiological and epidemiological investigations implicated an imported irish soft unpasteurized cows' milk cheese as the vehicle of infection. a case-control study showed a statistically significant association between infection and consumption of the suspect cheese (p = 0.001). salmonella dublin was subseque ... | 1992 | 1468523 |
| some aspects of the epidemiology and control of salmonella typhimurium infection in outwintered suckler cows. | two outbreaks of salmonella typhimurium infections affected outwintered, spring-calving suckler cows in late pregnancy. the infections spread rapidly both within and between groups of stock on the affected farms, with morbidity in the infected groups varying from 14.5 per cent to over 60 per cent, and mortality in adult cattle varying from 0 to 14.3 per cent. prophylactic measures included the use of antibiotics and killed vaccines against escherichia coli, salmonella dublin, s typhimurium, and ... | 1992 | 1475896 |
| [salmonella dublin infections in cattle, a review]. | in this review salmonella dublin infections in cattle are discussed. pathogenic pathways and clinical symptoms in calves and cows are mentioned in addition to diagnostic methods like post mortem examination, bacteriological methods and serology. epidemiological aspects like carrier state in cows and the survival of the bacteria in the environment are discussed. without reliable tests to detect carriers, we suggest that control of this infection on farms must be based on prevention, improved hygi ... | 1992 | 1481196 |
| [protection from virulent salmonella groups b and d after the peroral immunization of chicks with a hybrid of salmonella typhimurium and salmonella dublin]. | chickens over 10 days old, infected orally with virulent salmonellae, were found to remain alive. histologic investigation showed the development of mild enteritis and more pronounced, lasting for more than two weeks, inflammation of the cecum, dissemination and focal lesions in the liver (granulomas, necrosis). in experiments on the oral immunization of 3-day old chickens the bivalent hybrid of s. typhimurium vaccine strain 274 and s. dublin induced only pronounced blast transformation in lymph ... | 1992 | 1481606 |
| a salmonella dublin virulence plasmid locus that affects bacterial growth under nutrient-limited conditions. | this paper reports the characterization of a new locus, vagc/vagd, on the virulence plasmid of salmonella dublin. strain g19, harbouring a tna insertion in vagc, exhibited reduced virulence although vagc was outside the 8 kb essential virulence region. g19 was also unable to grow on minimal-medium containing various sole carbon/energy sources, unlike the wild-type and plasmid-cured strains. sequencing of the locus revealed the presence of two orfs (vagc and vagd) which overlapped by one nucleoti ... | 1992 | 1495391 |
| salmonella typhimurium bacteremia: association with the virulence plasmid. | virulence plasmids, which are found in a small number of salmonella serotypes, greatly enhance the extraintestinal growth of salmonellae and lower the ld50 by 2-5 logs in experimental murine infections. to determine if virulence plasmids are important in the pathogenesis of salmonella bacteremia in humans, blood and fecal isolates of salmonella typhimurium from california were examined for the presence of a virulence plasmid. colony blots were done using a labeled probe made from the highly cons ... | 1992 | 1500749 |
| plasmid profile analysis, phage typing, and antibiotic sensitivity of salmonella dublin from clinical isolates in the united states. | one hundred clinical isolates of salmonella choleraesuis subsp. choleraesuis serovar dublin (salmonella dublin) were examined for phage sensitivity, antibiotic resistance patterns, and plasmid content. computer analysis of the lysis patterns observed by using 27 typing phages divided the s. dublin isolates into 26 groups. one lytic pattern (designated pattern 16) contained 52% of the isolates examined whereas 16 isolates had unique patterns, and nine patterns had fewer than ten members. although ... | 1992 | 1514237 |
| molecular evolutionary genetics of the cattle-adapted serovar salmonella dublin. | an electrophoretic analysis of allelic variation at 24 enzyme loci among 170 isolates of the serovar salmonella dublin (serotype 1,9,12[vi]:g,p:-) identified three electrophoretic types (du 1, du 3, and du 4), marking three closely related clones, one of which (du 1) is globally distributed and was represented by 95% of the randomly selected isolates. all but 1 of 114 nonmotile isolates of serotype 1,9,12:-:- recovered from cattle and swine in the united states were genotypically du 1. the virul ... | 1992 | 1592813 |
| identification of a multimer resolution system involved in stabilization of the salmonella dublin virulence plasmid psdl2. | the salmonella dublin virulence plasmid psdl2 is a low-copy-number plasmid that is highly conserved in its host. deletion of the 8-kb ecori c fragment downstream of the virulence region leads to plasmid instability and formation of multimers. we identified a multimer resolution system in the ecori c fragment composed of a trans-acting resolvase gene and a cis-acting resolution site. the resolvase gene, rsd, maps within a 2-kb ecorv fragment and appears to be part of a multicistronic unit togethe ... | 1991 | 1653217 |
| characterization of three proteins expressed from the virulence region of plasmid psdl2 in salmonella dublin. | infection of both cattle and humans with salmonella dublin can result in septicemia and death. like many nontyphoid salmonella species that cause disease, s. dublin contains a cryptic plasmid (psdl2) that is required for the full expression of virulence. transposon mutagenesis of psdl2 defined a 4.1-kb ecori region that is necessary for the development of a systemic infection in balb/c mice. this ecori fragment was cloned into an expression vector (pel11), and three proteins produced from this r ... | 1991 | 1654301 |
| tn1725 transposon mutagenesis of 9-18 delta 7, an ecori deletion derivative of salmonella dublin lane plasmid psdl2. | a 37.5-kb derivative of the salmonella dublin virulence plasmid psdl2 was subjected to mutagenesis with the transposon tn1725. fifty-two insertions were mapped, and the mutants were tested for their ability to restore virulence to a plasmid-free strain of s. dublin. twenty-nine of these inserts could not restore full virulence and thus define nine regions of the plasmid essential for virulence. deletion of a 4.5-kb region by bal31 nuclease resulted in a 33-kb derivative that maintained full viru ... | 1991 | 1657789 |
| peyer's patches in experimental salmonella dublin infection in calves. microvascular and epithelial changes contributing to atrophy of lymphoid follicles. | six calves were infected per os with salmonella dublin and killed nine hours to seven days later. early changes included occlusion of capillaries with a hyaline material, particularly in the ileal peyer's patch (pp). central areas of the follicles contained hemorrhages and edema. in later stages the follicle-associated epithelium (fae) of both the jejunal and ileal pp was fused with the adjacent epithelium and the follicles were collapsed. as judged from 5'nucleotidase histochemistry, follicles ... | 1990 | 1690558 |
| recognition of a hepatitis b virus nucleocapsid t-cell epitope expressed as a fusion protein with the subunit b of escherichia coli heat labile enterotoxin in attenuated salmonellae. | two overlapping t-cell sites of the nucleocapsid antigen (hbc) of hepatitis b virus (hbv) (amino acids (aa) 120-140) and a b-cell epitope of the pre-s(2) region of the hbv surface antigen (aa 133-140) were expressed as a fusion protein with the subunit b of escherichia coli heat labile enterotoxin (lt-b) in attenuated salmonellae (aroa salmonella dublin sl1438). when balb/c (haplotype h-2d) mice were fed salmonellae expressing lt-b or the lt-b/hbv fusion protein they developed serum igg anti-lt- ... | 1990 | 1708183 |
| identification of salmonellae with the 4-methylumbelliferyl caprilate fluorescence test. | we have tested 750 salmonella strains and 130 strains of other species of the family enterbacteriaceae with the 4-methylumbelliferyl caprilate reagent (mucap) test. the mucap test is a fluorescence test for rapid identification of salmonella strains. the non-salmonella strains were strains sent for identification as suspected salmonella strains and thus have phenotypes similar to those of salmonella strains. all 748 tested salmonella strains of subgroups i, ii, iii, and iv were positive in the m ... | 1991 | 1774276 |
| persistent experimental salmonella dublin intramammary infection in dairy cows. | experimental intramammary infections were induced in five post-parturient holstein cows by inoculation of low numbers (5000 colony forming units) of virulent salmonella dublin via the teat canal of mammary gland quarters. rectal temperature, pulse and respiratory rates, milk yield, and milk quality as assessed by the california mastitis test (cmt) and somatic cell counts (scc) were recorded every 12 hours at milking. bacteriologic cultures of foremilk quarter samples and feces were obtained dail ... | 1991 | 1779428 |
| fate of unirradiated salmonella in irradiated mechanically deboned chicken meat. | mechanically deboned chicken meat was irradiated at 0, 1.25 and 2.50 kgy (cesium 137) and inoculated with salmonella dublin atcc 15480, salmonella enteritidis atcc 9186 or salmonella typhimurium atcc 14028. samples were then stored at 5 degrees c and 10 degrees c and were subjected to microbiological analysis directly after irradiation and inoculation (time 0), and after 24, 72, 120, 168 and 216 h of storage. samples stored at 20 degrees c were examined at time 0 and after 6, 12 and 24 h of stor ... | 1991 | 1790108 |
| genetic analysis of homology between the virulence plasmids of salmonella dublin and yersinia pseudotuberculosis. | two segments within the virulence region of salmonella dublin plasmid psdl2 that were homologous to regions on yersinia pseudotuberculosis plasmid pib1 were located with regard to the four known genes (vsda, vsdb, vsdc, and vsdd) of psdl2. one segment mapped upstream of vsda within an insertion element related to is630 of shigella sonnei; the second was confined to a 45-bp sequence containing an inverted repeat between vsdc and vsdd. on pib1, both areas were located in an intergenic region upstr ... | 1991 | 1840573 |
| salmonella dublin experimental infection in calves: protection after oral immunization with an auxotrophic aroa live vaccine. | salmonella dublin strain sl5631, which is auxotrophic for p-amino-benzoic acid and 2,3-dihydroxybenzoate because of a deleted aroa gene, was given orally in a dose of 10(10) live bacteria to 6 calves 5-7 weeks old. the calves tolerated the strain well, had a transient mucoid diarrhea and sacrificed animals showed a moderate acute inflammation in the ileum on day 2. the salmonella strain was seen lining the mucosal epithelium using immunohistopathology. already in calves sacrificed on day 6 the d ... | 1991 | 1853671 |
| experimental oral salmonella dublin infection in calves. a bacteriological and pathological study. | groups of calves (6-7, 12-14 and 24-28 weeks old) were orally infected with different numbers of the virulent salmonella dublin strain sva47. for the 6-7 weeks old calves the ld50-dose was estimated to be 1 x 10(7) bacteria. a dose of 10(9) bacteria was lethal within 24 hrs with the calves dying from septicemia and an acute necrotizing panenteritis. calves 12-14 weeks old given 2 x 10(10) sva47 bacteria succumbed to a progressive enteritis within one week. the 24-28 weeks old calves were resista ... | 1991 | 1858456 |
| antigenic homology among gram-negative organisms isolated from cattle with clinical mastitis. | this study examined the degree of serologic homology among mastitis pathogens. antibodies were raised against the rc mutant, escherichia coli o111:b4 (strain j5) and affinity purified against lipopolysaccharide derived from the ra mutant, salmonella typhimurium tv119. these antibodies reacted with a battery of unrelated gram-negative bacteria in whole cell elisa. bacteria with strong cross-reactions included a heterologous, smooth e. coli, salmonella dublin, s. typhimurium, salmonella newport, a ... | 1991 | 1907303 |
| a survey of antimicrobial resistance in salmonellae isolated from animals in england and wales during 1984-1987. | resistance to 14 antimicrobial substances was tested in 18,647 salmonella strains isolated from animals, their environment and from animal feeds during the period 1984-1987. of the 2284 salmonella dublin strains the percentage sensitive to all the antimicrobial substances ranged from 18.1 to 26.8. resistance to the higher concentration of streptomycin (s25) ranged from 1.9 to 6.4%, whereas the corresponding figures when the lower concentration (s10) was used were 32.3 and 63.8%. resistance to th ... | 1991 | 1913133 |
| non-typhoid salmonella bacteraemia in greater copenhagen 1984 to 1988. | a retrospective survey of non-typhoid salmonella bacteraemia in the period 1984 to 1988 was carried out by the five departments of clinical microbiology in greater copenhagen. a total of 168 patients were identified. a gradual increase was observed from 11 cases in 1984 to 58 cases in 1988. the corresponding incidence per 100,000 inhabitants in copenhagen rose from 0.9 in 1984 to 5.0 in 1988. during the same period the total registered incidence of human salmonella infections in denmark increase ... | 1991 | 1915383 |
| growth regulation of a salmonella plasmid gene essential for virulence. | the salmonella dublin plasmid gene vsdc is essential for virulence. we have constructed a vsdc-lacz translational fusion to demonstrate that vsdc is selectively expressed during the stationary phase of bacterial cell growth. this pattern of expression has been confirmed by mrna hybridization studies. carbon starvation is able to induce vsdc expression by limiting bacterial growth. the expression of vsdc is dependent upon an upstream gene, vsda, whose gene product possesses significant amino-term ... | 1991 | 1938884 |
| immunizing efficacy of aromatic-dependent salmonella dublin in mice and calves. | mice immunized with an aromatic-dependent (aro-) s. dublin strain cs101 by either the intraperitoneal (i.p.) or oral route, were protected against oral challenge with a virulent s. dublin strain cs90, the degree of protection being the greatest when mice had received 3 immunizing doses at weekly intervals. mice immunized with an aromatic-dependent (aro-) s. typhimurium strain cs332 by the i.p. or oral routes were protected against challenge with virulent s. dublin strain cs90 at 1 or 2 weeks but ... | 1991 | 1959320 |
| salmonella dublin infection of a prosthetic vascular graft successfully treated with ciprofloxacin. | the treatment of an infected vascular graft invariably requires an extra-anatomic bypass through a non-infected tissue plane, as simple drainage with antibiotics is rarely successful. we report a case in which an established infection of a prosthetic vascular graft with salmonella dublin was successfully treated with simple drainage and a prolonged course of oral ciprofloxacin. | 1991 | 2026892 |
| human infection with salmonella dublin. | twenty-seven cases of human infection with salmonella dublin were identified over a 12-year period at the university of california at san diego-affiliated hospitals. important epidemiologic risk factors were the ingestion of unpasteurized dairy products or treatment with nutritional therapy that included raw calf-liver extracts. nearly all patients had underlying chronic diseases. like salmonella choleraesuis, s. dublin infections were associated with a high incidence of bacteremia (91%), metast ... | 1991 | 2030643 |
| expression and immunogenicity of a streptococcal m protein epitope inserted in salmonella flagellin. | a synthetic 48-bp oligonucleotide specifying the n-terminal 15 amino acids of m protein of streptococcus pyogenes type 5 (plus a cta codon, to terminate translation of genes with the insert in reverse orientation) was inserted by blunt-end ligation at the site of the 48-bp ecorv deletion in the salmonella flagellin gene in plasmid pls408 (s. m. c. newton, c. o. jacob, and b. a. d. stocker, science 244: 70-72, 1989). the resulting plasmid was transferred from escherichia coli via a restriction-ne ... | 1991 | 2037377 |
| molecular analysis of the virulence locus of the salmonella dublin plasmid psdl2. | the virulence properties of various non-typhoid salmonella serotypes depend on the presence of large plasmids 60-100 kb in size. we have shown previously that the virulence region on the 80 kb plasmid psdl2 of salmonella dublin lane maps within a 14kb sali fragment. in this report we show that an 8.2 kb region within this fragment is sufficient to express lethal disease in balb/c mice. sequence analysis of this segment revealed six sequential open reading frames designated vsda-f, which encode p ... | 1991 | 2041471 |
| identification of proteins expressed by the essential virulence region of the salmonella dublin plasmid. | an 8 kilobase pair (kb) fragment from the salmonella dublin 2229 plasmid is sufficient to restore virulence for mice to a cured strain of s. dublin. deletion analysis of this virulence fragment identified at least one specific region required for virulence expression. plasmid-directed protein synthesis in minicells has indicated the presence of at least four genes within the essential virulence region of the s. dublin plasmid, encoding proteins of 70, 33, 30 and 26 kda. analysis of the proteins ... | 1990 | 2077345 |
| use of elisa for detection of immunoglobulins g and m that recognize salmonella dublin lipopolysaccharide for prediction of carrier status in cattle. | immunoglobulin reactions to salmonella dublin in serum and milk from 4 groups of lactating cows were measured by an indirect elisa. the groups consisted of (1) cows that were natural carriers of s dublin in the mammary gland, (2) experimentally infected cows that did not become carriers, (3) cows inoculated with a commercial s dublin bacterin, and (4) cows used as s dublin-negative controls. milk and serum samples were obtained at monthly intervals. models for predicting carrier status were deve ... | 1990 | 2085214 |
| immunity induced by live attenuated salmonella vaccines. | studies on the degree and specificity of protection conferred by immunization with aroa salmonella live vaccines in balb/c mice are described. animals were immunized i.v. and challenged orally 3 months later to ensure that the vaccine had been cleared from the tissues. vaccination with salmonella typhimurium aroa sl3261 conferred very good protection against virulent s. typhimurium c5 (over 10,000 x ld50). the specificity of cross protection was studied using s. typhimurium, salmonella enteritid ... | 1990 | 2101466 |
| the virulence plasmid of salmonella dublin: detailed restriction map and analysis by transposon mutagenesis. | a detailed restriction map of the virulence plasmid of salmonella dublin has been determined and used for comparison with the virulence plasmid from s. typhimurium. two regions were identified which appeared to be similar based on blotting and restriction data. one, of about 22 kb, encompassed the virulence region; the other, of about 8 kb, was outside it. the locations of 259 transposon insertions on the s. dublin plasmid were determined and related to their effect on virulence. one gene involv ... | 1990 | 2166770 |
| transposon insertion mutagenesis of a genetic region encoding serum resistance in an 80 kb plasmid of salmonella dublin. | using transposon insertion mutagenesis with tn1 or tn5, we obtained salmonella dublin mutant strains that showed either diminished serum resistance (five mutants) or diminished mouse lethality (two mutants). detailed restriction cleavage analysis to determine the single sites of transposon insertion in an 80 kb plasmid (pte800) indicated that a region for serum resistance was located within a 3.0 kb region of the sali cleavage fragment 5 and the hindiii fragment 2, while the region for mouse let ... | 1990 | 2178177 |
| the prevalence of plasmids in danish bovine and human isolates of salmonella dublin. | nine different plasmid profiles were demonstrated among 135 danish isolates of salmonella dublin, with 58% of the strains enclosed in the major profile group. two strains did not carry any plasmids. the plasmid profiles of 129 strains (96%) were made up of one or more of only four plasmids of approximate sizes 98 kb, 80 kb, 4.0 kb, and 3.8 kb. there was no significant difference in the prevalence of different plasmid profile groups between isolates from cattle and man. the serovar-specific and v ... | 1990 | 2206517 |
| homologous dna sequences on the virulence plasmids of pathogenic yersinia and salmonella dublin lane. | yersinia and salmonella harbour plasmids that encode traits important for virulence, enabling both pathogenic genera to survive and grow in cells of the reticulo-endothelial organs during systemic infections. we have detected dna homology between the salmonella dublin virulence plasmid psdl2 and the plasmids of the pathogenic yersinia species pestis, pseudotuberculosis, and enterocolitica. three regions of psdl2 were found to share homology with the virulence plasmid pib1 of yersinia pseudotuber ... | 1990 | 2215215 |
| hepatitis b virus nucleocapsid/pre-s2 fusion proteins expressed in attenuated salmonella for oral vaccination. | hybrid hbv nucleocapsid-pre-s(2) fusion proteins were stably expressed in several aromatic-dependent attenuated salmonella typhimurium and salmonella dublin strains. when these live recombinant bacteria were administered i.p. to balb/c mice they induced high titer anti-hepatitis b virus core ag (hbc) and detectable anti-pre-s2 serum antibodies. upon oral feeding of the recombinant salmonellae to mice, the rate of seroconversion to anti-hbc was dependent on the salmonella strain used. with the be ... | 1990 | 2258619 |
| salmonella infection in total hip replacement: tests to predict the outcome of antimicrobial therapy. | we report a hematogenous implant infection with salmonella dublin in a renal transplant patient with total hip replacement. a 16-month treatment with cotrimoxazole failed, as evidenced by culture and electron microscopy, despite persisting low mic after therapy. data from a foreign body animal model and in vitro tests, which take into account the properties of adhering and stationary-phase bacteria, explain the failure of a long-term treatment with cotrimoxazole. the patient was subsequently cur ... | 1990 | 2259871 |
| [bacteremia caused by zoonotic salmonella types in greater copenhagen in 1984-1988]. | the five departments of clinical microbiology in greater copenhagen have together carried out a retrospective review of bacteraemia caused by the zoonotic salmonella serotypes in the period 1984-1988 in the municipalities of copenhagen and frederiksberg and in the county of copenhagen. a gradual increase in frequency was observed from 11 cases in 1984 to 58 cases in 1988. the serotype most commonly isolated was salmonella dublin followed by salmonella enteritidis and salmonella typhimurium. s. d ... | 1990 | 2309361 |
| [total hip prosthesis infected with salmonella dublin]. | in 1983, the bovine-specific salmonella dublin was demonstrated in man in denmark. the organism is frequently isolated from blood but rarely causes gastroenteritis. the frequency of infection following hip replacement is less than 2% with staphylococcus aureus as the commonest agent while gram-negative rods are rarer but are considered to be more serious. infection around a hip replacement frequently implies that the prosthesis must be removed. a case in which a hip replacement was infected with ... | 1990 | 2309373 |
| correlation between the presence of sequences homologous to the vir region of salmonella dublin plasmid psdl2 and the virulence of twenty-two salmonella serotypes in mice. | large plasmids encoding important virulence properties have been found in several salmonella serotypes. we have studied the relationship between the presence of a highly conserved 4-kilobase (kb) ecori fragment from the plasmid virulence region and pathogenicity for mice of 53 isolates representing 22 serotypes of salmonella. only strains possessing the homologous 4-kb region were virulent for mice. in addition, we transferred the virulence plasmid from s. dublin into nine different serotypes, i ... | 1990 | 2323813 |
| successful treatment using gentamicin liposomes of salmonella dublin infections in mice. | gentamicin entrapped within stable multilamellar liposomes was used to treat mice after they were infected per os with salmonella dublin. of 10 mice, 8 survived after a single intravenous (i.v.) injection of 2 mg of gentamicin liposomes per kg compared with 0 of 10 treated with the same amount of free gentamicin. all mice survived after treatment with a single i.v. or intraperitoneal injection of 20 mg of gentamicin liposomes per kg, whereas that dose of free drug was completely ineffective and ... | 1990 | 2327780 |