| trace elements in major marketed marine bivalves from six northern coastal cities of china: concentrations and risk assessment for human health. | one hundred and fifty nine samples of nine edible bivalve species (argopecten irradians, chlamys farreri, crassostrea virginica, lasaea nipponica, meretrix meretrix, mytilus edulis, ruditapes philippinarum, scapharca subcrenata and sinonovacula constricta) were randomly collected from eight local seafood markets in six big cities (dalian, qingdao, rizhao, weifang, weihai and yantai) in the northern coastal areas of china for the investigation of trace element contamination. as, cd, cr, cu, hg, p ... | 2014 | 25128644 |
| identification, expression, and innate immune responses of two insulin-like peptide genes in the razor clam sinonovacula constricta. | insulin-like peptide (ilp) has emerged as a cell regulatory factor with multiple functions in vertebrates and invertebrates. in the present study, we identified and characterized two ilp genes, ilp1 and ilp2, in the razor clam sinonovacula constricta. both ilps have a signal peptide and a mature domain consisting of six strictly conserved cysteines. the tertiary structure is divided into three main α-helices with a c-domain loop that separates helix 1 from helix 2. both of ilps were found to be ... | 2016 | 26980611 |
| complement c3 gene: expression characterization and innate immune response in razor clam sinonovacula constricta. | complement component 3 (c3) is central to the complement system, playing an important role in immune defense, immune regulation and immune pathology. several c3 genes have been characterized in invertebrates but very few in shellfish. the c3 gene was identified from the razor clam sinonovacula constricta, referred to here as sc-c3. it was found to be highly homologous with the c3 gene of ruditapes decussatus. all eight model motifs of the c3 gene were found to be included in the thiolester bond ... | 2016 | 27231190 |
| expression of a novel complement c3 gene in the razor clam sinonovacula constricta and its role in innate immune response and hemolysis. | complement component 3 (c3) is a core component of the complement system, and directly participates in immune regulation and immune defense. isoforms of c3 have been reported in several species of vertebrate, but invertebrates, and more specifically clams, have been less well studied. an isoform of c3, named scc3-2, was identified in sinonovacula constricta (chinese razor clam). scc3-2 included eight conserved regions, a thioester bond and two predicted junction sites (α-β and α-γ). the gene was ... | 2017 | 28377201 |
| investigation on the differences of accumulating escherichia coli in three types of shellfish species, involving in the environmental factors. | this study investigated accumulation of escherichia coli and aerobic colony count in three types of shellfish species. the results indicated that the capability of accumulating e. coli and aerobic colony count for sinonovacula constricta was stronger than that for meretrix meretrix and tegillarca granosa, and capability of accumulating e. coli for m. meretrix was slightly stronger than that for t. granosa. however, no significant difference was observed in the capability of accumulating aerobic ... | 2016 | 27283878 |
| parabolic stationing method application to intertidal shellfish (sinonovacula constricta) and growing waters microbiological monitoring in yueqing bay, china. | this study reported microbiological monitoring by parabolic stationing method for s. constricta and their growing waters, and application for the first time in yueqing bay, china. the results indicated that numbers of escherichia coli and aerobic colony count in s. constricta flesh showed an approximate 'parabola' distribution across the whole shellfish bed. numbers of faecal coliform in seawater samples were significantly higher in the top of the bay, and lower in the lower half of the bay, whi ... | 2017 | 28040249 |
| identification of angiotensin i-converting enzyme inhibitory peptides derived from enzymatic hydrolysates of razor clam sinonovacula constricta. | angiotensin i-converting enzyme (ace) inhibitory activity of razor clam hydrolysates produced using five proteases, namely, pepsin, trypsin, alcalase, flavourzyme and proteases from actinomucor elegans t3 was investigated. flavourzyme hydrolysate showed the highest level of degree of hydrolysis (dh) (45.87%) followed by a. elegans t3 proteases hydrolysate (37.84%) and alcalase (30.55%). the a. elegans t3 proteases was observed to be more effective in generating small peptides with ace-inhibitory ... | 2016 | 27271639 |
| occurrence of polybrominated diphenyl ethers in fish and shellfish downstream from electronic-waste recycling plants. | we measured 39 polybrominated diphenyl ethers (pbdes) in the muscle tissue of three species of fish (sciaenops ocellatus, sparus macrocephalus, and lateolabraxjaponicus) and four species of shellfish (tegillarca granosa, cyclina sinensis, sinonovacula constricta, and ostrea cucullata) that were collected downstream of electronic-waste recycling plants in taizhou, china. a total of 24 pbde congeners (pbde24) in the samples were detected. the sigma pbde24 (total pbde) ranged from 545.4 to 1688.7 n ... | 2010 | 20608509 |
| sequence analysis and expression of a cdna clone encoding tropomysin in sinonovacula constricta. | shellfish can cause severe anaphylactic reactions. tropomyosin has been assumed partly responsible for the cross-reactivity among shellfish and other invertebrates. in this study, cdna of sinonovacula constricta was amplified by rt-pcr and 3'-race from total rna. the obtained tropomyosin cdna included an open reading frame coding for 284 amino acids. the deduced amino acid sequence of the corresponding protein shared high identity with other allergenic tropomyosins. expression of the recombinant ... | 2009 | 18046627 |
| a new gymnophallid trematode from the intestine of mice infected with metacercariae from the razor clam sinonovacula constricta. | meiogymnophallus sinonovaculae n. sp. (digenea: gymnophallidae) is described from metacercariae found in the razor clam, sinonovacula constricta, in the republic of korea, and adults recovered from the small intestine of experimentally infected mice. the worms are characterized by paired clusters of vitelline follicles, a well-developed pars prostatica with abundant prostate cells, a voluminous undivided seminal vesicle (in adults and metacercariae), and large, grouped, domelike sensory papillae ... | 2007 | 17436952 |
| investigation of organochlorine pesticides (ocps) in mollusks collected from coastal sites along the chinese bohai sea from 2002 to 2004. | mollusks living in seas can accumulate organochlorine pesticides (ocps). the residue levels of selected ocps: dichlorodiphenyltrichloroethane (p,p'-ddt, o,p'-ddt, p,p'-dde, p,p'-ddd), hexachlorocyclohexanes (alpha-, beta-, gamma- and delta-hch) in gastropod and bivalve species collected from ten coastal cites along the chinese bohai sea were investigated from 2002 to 2004. the species included nine kinds of mollusks: rapana venosa, neverita didyma, scapharca subcrenata, mytilus edulis, amusium, ... | 2007 | 16978749 |
| [bacterial flora composition and its dynamics in tidal-flat sinonovacula constricta aquaculture area]. | in this study, the bacteria from the mud in tidal-flat sinonovacula constricta aquaculture area were isolated each month from march to december, 2002, and the temporal and spatial distribution of heterotrophic bacteria, ammonifying bacteria, denitrifying bacteria, and sulphate reducing bacteria were analyzed. the results showed that all the 515 isolated bacteria mainly belonged to 1 family and 13 genera. the bacterial flora in different layers of the mud was almost consistent, while the composit ... | 2005 | 16252888 |
| [carrying capacity of shellfish culture in dadeng island sea area of xiamen]. | to fully and rationally exploit local living marine resources while have a sustainable, efficient and healthy development of shellfish culture in the dadeng island sea area of xiamen, this paper determined and analyzed the related model parameters of this area, including chlorophyll a, primary productivity, phytoplankton organic carbon tent, wild filter feeder yields in subtidal and intertidal zones and suspension culture area, cultured shellfish filtration rate and organic carbon content, shell ... | 2005 | 16110680 |
| immune bioactivity in shellfish toward serum-free cultured human cell lines. | the biologically functional effect of eight kinds of hot-water extracts of shellfish on cultured human cell lines was examined in a serum-free medium model. meretrix lusoria and sinonovacula constricta extracts enhanced igm secretion of both hybridoma hb4c5 and si102 cells when cultured with the respective extracts. the purified principle exhibited remarked activity in the adsorbed fraction in hydroxyapatite and concanavalin a columns. the extracts of corbicula fluminea, crassostreas gigas, mere ... | 1997 | 9028032 |
| the presence of genogroup ii norovirus in retail shellfish from seven coastal cities in china. | noroviruses (novs) are commonly occurring pathogens that cause gastroenteritis. outbreaks of viral diseases have often been ascribed to the consumption of contaminated shellfish. our objective was to evaluate the presence and contamination levels of nov in shellfish sold at seafood markets in china. we tested 840 shellfish samples (crassostrea gigas, mytilus edulis, azumapecten farreri, sinonovacula constricta, scapharca subcrenata, ruditapes philippinarum) that were collected from seven cities ... | 2013 | 23412724 |
| genetic considerations for mollusk production in aquaculture: current state of knowledge. | in 2012, world mollusk production in aquaculture reached a volume of 15,171,000 tons, representing 23% of total aquaculture production and positioning mollusks as the second most important category of aquaculture products (fishes are the first). clams and oysters are the mollusk species with the highest production levels, followed in descending order by mussels, scallops, and abalones. in view of the increasing importance attached to genetic information on aquaculture, which can help with good m ... | 2014 | 25540651 |
| simultaneous multiplexed stripping voltammetric monitoring of marine toxins in seafood based on distinguishable metal nanocluster-labeled molecular tags. | marine toxins from microscopic algae can accumulate through the food chain and cause various neurological and gastrointestinal illnesses for human health. herein, we designed a new ultrasensitive multiplexed immunoassay protocol for simultaneous electrochemical determination of brevetoxin b (btx-2) and dinophysistoxin-1 (dtx-1) in seafood using distinguishable metal nanocluster-labeled molecular tags as traces on bifunctionalized magnetic capture probes. to construct such a bifunctionalized prob ... | 2012 | 22906150 |
| identification of cathepsin b in the razor clam sinonovacula constricta and its role in innate immune responses. | cathepsin b, a lysosomal cysteine protease, has drawn much attention in vertebrates. however, very little is known about the functions of cathepsin b in bivalves. in this study, we identified the cathepsin b gene in the razor clam sinonovacula constricta. the protein has a typical cysteine protease structure, comprising a 15-residue putative signal peptide, a 75-residue propeptide and a 249-residue mature domain. in the mature domain, there is an occluding loop, an oxyanion hole (gln) and a cata ... | 2013 | 23632254 |
| molecular characterization and expression analysis of four cathepsin l genes in the razor clam, sinonovacula constricta. | cathepsin l (ctsl) is a lysosomal cysteine protease involved in immune responses in vertebrates. however, few studies exist regarding the role of cathepsin l in bivalves. in this study, we isolated and characterized four cathepsin l genes from the razor clam sinonovacula constricta, referred to as ctsl1, ctsl2, ctsl3 and ctsl4. these four genes contained typical papain-like cysteine protease structure and enzyme activity sites with erwnin-like and gnfd-like motifs in the proregion domain and an ... | 2013 | 23765116 |
| identification, expression, and responses to bacterial challenge of the cathepsin c gene from the razor clam sinonovacula constricta. | cathepsin c (dipeptidyl-peptidasei, dppi) is a lysosomal cysteine proteinase that belongs to the papain superfamily, and it is involved in protein degradation and proenzyme activation. however, very little is known about the function of cathepsin c in bivalves. in the present study, we identified the cathepsin c gene in the razor clam sinonovacula constricta (sc-ctsc). the full-length sc-ctsc cdna contained a complete open reading frame (orf) of 1371 nt encoding 456 amino acids, a 98 bp 5' utr, ... | 2014 | 24792213 |
| development of molecular resources for an intertidal clam, sinonovacula constricta, using 454 transcriptome sequencing. | the razor clam sinonovacula constricta is a benthic intertidal bivalve species with important commercial value. despite its economic importance, knowledge of its transcriptome is scarce. next generation sequencing technologies offer rapid and efficient tools for generating large numbers of sequences, which can be used to characterize the transcriptome, to develop effective molecular markers and to identify genes associated with growth, a key breeding trait. | 2013 | 23935831 |
| trichodinid ectoparasites (ciliophora: peritrichida) from the gills of mariculture molluscs in china, with the descriptions of four news species of trichodina ehrenberg, 1838. | four new species of the genus trichodina, found on gills of marine molluscs from aquaculture beds off the coast of qingdao (tsingao), china, are described: t. chlamydis n. sp. from azumapecten (chlamys)farreri (pectinidae), t. sinonovaculae n. sp. from sinonovacula constricta (solenidae), t. liana n. sp. from solen (plectosolen) gracilis (solenidae) and t. meretricis n. sp. from meretrix meretrix (veneridae). the descriptions presented here include morphometric data obtained from examinations of ... | 1999 | 10613541 |