| quantification of pyrenophora teres in infected barley leaves using real-time pcr. | net blotch is a barley foliar disease caused by two forms of pyrenophora teres: pyrenophora teres f. teres (ptt) and pyrenophora teres f. maculata (ptm). to monitor and quantify their occurrence during the growing season, diagnostic system based on real-time pcr was developed. taqman mgb (minor groove binder) primers and probes were designed that showed high specificity for each of the two forms of p. teres. as a host plant internal standard, taqman mgb primers and probe based on racb gene seque ... | 2006 | 16806544 |