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a region of barley chromosome 6h harbors multiple major genes associated with net type net blotch resistance.net type net blotch (ntnb), caused by pyrenophora teres f. teres drechs., is prevalent in barley growing regions worldwide. a population of 118 doubled haploid (dh) lines developed from a cross between barley cultivars 'rika' and 'kombar' were used to evaluate resistance to ntnb due to their differential reaction to various isolates of p. teres f. teres. rika was resistant to p. teres f. teres isolate 15a and susceptible to isolate 6a. conversely, kombar was resistant to 6a, but susceptible to 1 ...200818712341
mapping net form net blotch and septoria speckled leaf blotch resistance loci in barley.septoria speckled leaf blotch (sslb), caused by septoria passerinii sacc., and net form net blotch (nb), caused by pyrenophora teres f. teres drechsler, are fungal diseases that decrease the yields of barley in the upper midwest. an effective way to manage these diseases is to plant resistant cultivars. to characterize the genetics of resistance to both pathogens, two advanced barley breeding lines, one resistant to nb (m120) and another resistant to sslb (sep2-72), were crossed, creating a popu ...201019968552
a first genome assembly of the barley fungal pathogen pyrenophora teres f. teres.pyrenophora teres f. teres is a necrotrophic fungal pathogen and the cause of one of barley's most important diseases, net form of net blotch. here we report the first genome assembly for this species based solely on short solexa sequencing reads of isolate 0-1. the assembly was validated by comparison to bac sequences, ests, orthologous genes and by pcr, and complemented by cytogenetic karyotyping and the first genome-wide genetic map for p. teres f. teres.201021067574
permanent genetic resources added to molecular ecology resources database 1 february 2011-31 march 2011.this article documents the addition of 111 microsatellite marker loci to the molecular ecology resources database. loci were developed for the following species: acipenser oxyrinchus desotoi, anopheles nuneztovari sensu lato, asellus aquaticus, calopteryx splendens, calopteryx virgo, centaurea aspera, centaurea seridis, chilina dombeyana, proctoeces cf. lintoni and pyrenophora teres f. teres.201121627775
comparative virulence of pyrenophora teres f. teres from syria and tunisia and screening for resistance sources in barley: implications for breeding.aims: to investigate the pathogenic diversity and virulence groups among pyrenophora teres f. teres isolates, sampled from syria and tunisia, and to identify the most effective source of resistance in barley that could be used in breeding programs to control net blotch in both countries. methods and results: 104 isolates pyrenophora teres f. teres were collected from barley in different agro-ecological zones of tunisia and syria. their virulence was evaluated using 14 barley genotypes as diffe ...201121781138
pyrenophora teres: profile of an increasingly damaging barley pathogen.pyrenophora teres, causal agent of net blotch of barley, exists in two forms, designated p. teres f. teres and p. teres f. maculata, which induce net form net blotch (nfnb) and spot form net blotch (sfnb), respectively. significantly more work has been performed on the net form than on the spot form although recent activity in spot form research has increased because of epidemics of sfnb in barley-producing regions. genetic studies have demonstrated that nfnb resistance in barley is present in b ...201121118345
mating type locus-specific polymerase chain reaction markers for differentiation of pyrenophora teres f. teres and p. teres f. maculata, the causal agents of barley net blotch.fourteen single nucleotide polymorphisms (snps) were identified at the mating type (mat) loci of pyrenophora teres f. teres (ptt), which causes net form (nf) net blotch, and p. teres f. maculata (ptm), which causes spot form (sf) net blotch of barley. mat-specific snp primers were developed for polymerase chain reaction (pcr) and the two forms were differentiated by distinct pcr products: pttmat1-1 (1,143 bp) and pttmat1-2 (1,421 bp) for nf mat1-1 and mat1-2 isolates; ptmmat1-1 (194 bp) and ptmm ...201020731534
identification of molecular genetic markers in pyrenophora teres f. teres associated with low virulence on 'harbin' barley.abstract two isolates of the barley net blotch pathogen (pyrenophora teres f. teres), one possessing high virulence (0-1) and the other possessing low virulence (15a) on the barley cultivar harbin, were crossed and the progeny of the mating were isolated. conidia from cultures of the parent and progeny isolates were used as inoculum to determine the inheritance of virulence in the pathogen. of the 82 progeny tested, 42 exhibited high virulence and 40 exhibited low virulence on 'harbin' barley. t ...199918944793
genetic mapping of pyrenophora teres f. teres genes conferring avirulence on barley.a pyrenophora teres f. teres cross between isolates 0-1 and 15a was used to evaluate the genetics of avirulence associated with barley lines canadian lake shore (cls), tifang, and prato. 15a is avirulent on tifang and cls, but virulent on prato. conversely, 0-1 is avirulent on prato, but virulent on tifang and cls. avirulence:virulence on tifang and cls segregated 1:1, whereas avirulence:virulence on prato segregated 3:1. an aflp-based linkage map was constructed and used to identify a single lo ...200717224286
quantification of pyrenophora teres in infected barley leaves using real-time pcr.net blotch is a barley foliar disease caused by two forms of pyrenophora teres: pyrenophora teres f. teres (ptt) and pyrenophora teres f. maculata (ptm). to monitor and quantify their occurrence during the growing season, diagnostic system based on real-time pcr was developed. taqman mgb (minor groove binder) primers and probes were designed that showed high specificity for each of the two forms of p. teres. as a host plant internal standard, taqman mgb primers and probe based on racb gene seque ...200616806544
[genetic control of virulence of pyrenophora teres drechs, the causative agent of net blotch in barley].the genetic control of virulence was studied in four isolates of the fungus pyrenophora teres f. teres, originating from various geographic regions in experiments with nine barley accessions, possessing known resistance genes. experiments were performed with the ascospore progeny of two crosses. the results of segregation for virulence in the progeny of direct crosses were confirmed by analysis of backcrosses and sib crosses. one to four genes for avirulence toward various barley genotypes were ...200516396454
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