| extracellular homopolysaccharides and oligosaccharides from intestinal lactobacilli. | to characterize lactobacilli isolated from the intestines of ducks or pigs with respect to the production of extracellular homopolysaccharides (hops) and oligosaccharides. | 2005 | 16108811 |
| identification and antimicrobial susceptibility of porcine bacteria that inhibit the growth of brachyspira hyodysenteriae in vitro. | to identify bacilli, lactic acid bacteria and bifidobacteria that inhibit the growth of brachyspira hyodysenteriae. | 2010 | 19778354 |
| identification of a new adhesin-like protein from lactobacillus mucosae me-340 with specific affinity to the human blood group a and b antigens. | to identify and characterize a new adhesin-like protein of probiotics that show specific adhesion to human blood group a and b antigens. | 2010 | 20408914 |
| lactic acid bacteria and yeasts associated with gowé production from sorghum in bénin. | to identify the dominant micro-organisms involved in the production of gowé, a fermented beverage, and to select the most appropriate species for starter culture development. | 2007 | 17650194 |
| isolation of surface (s) layer protein carrying lactobacillus species from porcine intestine and faeces and characterization of their adhesion properties to different host tissues. | surface-layer proteins (slps) of lactobacilli have been shown to confer tissue adherence. this study aimed to isolate and identify slps carrying lactobacillus species from the porcine intestine and faeces and to characterize these s-layer-expressing strains for their ability to adhere to the pig and human intestinal cells and to extracellular matrix (ecm) proteins. in total 99 strains, putatively belonging to the genus lactobacillus, were isolated as pure cultures. sds-page and a gene probe spec ... | 2007 | 17544232 |
| enterococcus and lactobacillus contamination of raw milk in a farm dairy environment. | enterococci and lactobacilli are ubiquitously found in the intestinal microflora of humans and animals. the aim of the present study was to determine the importance of bovine faeces as a source of these organisms in raw milk. one hundred and fifty six putative enterococci and 362 lactobacilli were isolated from bovine faeces (n=26), cows' teats, raw milk, the milking machine and the milking environment on one farm. the clonal relationships of each group were investigated using pulsed-field gel e ... | 2007 | 17189657 |
| quantitative evaluation of adhesion of lactobacilli isolated from human intestinal tissues to human colonic mucin using surface plasmon resonance (biacore assay). | to isolate lactobacilli from the mucus layer of the human intestine and evaluate their adhesion abilities using a biacore assay. | 2007 | 17184326 |
| alteration of the canine small-intestinal lactic acid bacterium microbiota by feeding of potential probiotics. | five potentially probiotic canine fecal lactic acid bacterium (lab) strains, lactobacillus fermentum lab8, lactobacillus salivarius lab9, weissella confusa lab10, lactobacillus rhamnosus lab11, and lactobacillus mucosae lab12, were fed to five permanently fistulated beagles for 7 days. the survival of the strains and their potential effects on the indigenous intestinal lab microbiota were monitored for 17 days. denaturing gradient gel electrophoresis (dgge) demonstrated that the five fed lab str ... | 2006 | 17021203 |
| distinctive intestinal lactobacillus communities in 6-month-old infants from rural malawi and southwestern finland. | our aim was to compare the composition and diversity of lactobacillus microbiota in infants living in malawi and southwestern finland. | 2015 | 26049782 |
| selection of potential probiotic lactobacilli from pig feces to be used as additives in pelleted feeding. | thirty-five isolates from pig feces were identified as lactobacillus reuteri (12 strains), lactobacillus mucosae (7), lactobacillus plantarum (6), lactobacillus kitasatonis (3), lactobacillus rossiae (2), lactobacillus ultunensis (2), lactobacillus crispatus (2), and lactobacillus intestinalis (1) by partial sequence analysis of the 16s rrna. all isolates were detected at 8-9 log cfu g(-1). preliminarily, strains were selected based on resistance to heat treatments (ca. 70 degrees c for 10 s). t ... | 2006 | 16844349 |
| development and assessment of a real-time pcr assay for rapid and sensitive detection of a novel thermotolerant bacterium, lactobacillus thermotolerans, in chicken feces. | a new real-time pcr assay was successfully developed using a taqman fluorescence probe for specific detection and enumeration of a novel bacterium, lactobacillus thermotolerans, in chicken feces. the specific primers and probe were designed based on the l. thermotolerans 16s rrna gene sequences, and these sequences were compared to those of all available 16s rrna genes in the genbank database. the assay, targeting 16s rrna gene, was evaluated using dna from a pure culture of l. thermotolerans, d ... | 2005 | 16085805 |
| the genetic diversity of lactic acid producing bacteria in the equine gastrointestinal tract. | seventy-two lactic acid producing bacterial isolates (excluding streptococci) were cultured from the gastrointestinal tract of six horses. two of the horses were orally dosed with raftilose to induce lactic acidosis and laminitis while the remaining four were maintained on a roughage diet. near complete 16s rdna was amplified by pcr from the genomic dna of each isolate. following rflp analysis with the restriction enzymes mboi, hhai and hinfi, the pcr products from the 18 isolates that produced ... | 2005 | 15953698 |
| isolation and characterisation of an equol-producing mixed microbial culture from a human faecal sample and its activity under gastrointestinal conditions. | only about one third of humans possess a microbiota capable of transforming the dietary isoflavone daidzein into equol. little is known about the dietary and physiological factors determining this ecological feature. in this study, the in vitro metabolism of daidzein by faecal samples from four human individuals was investigated. one culture produced the metabolites dihydrodaidzein and o-desmethylangolensin, another produced dihydrodaidzein and equol. from the latter, a stable and transferable m ... | 2004 | 15578160 |
| lactobacillus ingluviei sp. nov., isolated from the intestinal tract of pigeons. | lactic acid bacteria were isolated from the crop and intestines of pigeons. one group of strains, showing similar genomic patterns after screening with trna intergenic spacer pcr, could not be identified to the species level. sequencing of the 16s rrna gene of one representative strain revealed about 96% similarity to sequences from lactobacillus fermentum and lactobacillus mucosae. determination of the dna base composition, dna-dna hybridization experiments, sds-page of whole-cell proteins and ... | 2003 | 12656164 |
| lactobacillus mucosae sp. nov., a new species with in vitro mucus-binding activity isolated from pig intestine. | a new lactobacillus species from pig small intestine has been identified. in an attempt to isolate lactobacillus reuteri strains carrying the putative colonization-factor gene (mub, for mucus binding) a mub-derived gene probe was used to screen pig intestinal material. a number of isolates were obtained and primary characterization showed that they were gram-positive, catalase-negative, non-spore-forming, non-motile rods. growth occurred at 45 degrees c but not at 15 degrees c and the dna g+c co ... | 2000 | 10826811 |
| adherence of bacteria to mucus collected from different parts of the reproductive tract of heifers and cows. | in the present study, we examined the adherence of indigenous vaginal bacteria, probiotic strains, and metritis pathogens to mucus collected from different parts of the reproductive tracts of heifers and cows and compared their adherence with the bacterial adherence to mucus collected from the stomach and large intestine of pigs. most of the vaginal strains adhered to mucus collected from different parts of the reproductive tract and strongly adhered to gastric mucus, with the exception of lacto ... | 2013 | 24206354 |
| genome sequence of the heteropolysaccharide-producing strain lactobacillus mucosae dpc 6426. | exopolysaccharide-synthesizing lactobacillus mucosae dpc 6426 is a heterofermentative strain, which has demonstrated cholesterol-lowering properties in an animal model of lipid-driven atherosclerosis. the genome revealed a plethora of homologues linked to carbohydrate metabolism and mucin binding. | 2015 | 25593248 |
| draft genome sequence of the mannitol-producing strain lactobacillus mucosae crl573. | lactobacillus mucosae crl573, isolated from child fecal samples, efficiently converts fructose and/or sucrose into the low-calorie sugar mannitol when cultured in modified mrs medium at ph 5.0. also, the strain is capable of producing bacteriocin. the draft genome sequence of this strain with potential industrial applications is presented here. | 2014 | 25502678 |
| effects of the probiotic enterococcus faecium ncimb 10415 on selected lactic acid bacteria and enterobacteria in co-culture. | enterococcus faecium ncimb 10415 is used as a probiotic for piglets and has been shown to modify the porcine intestinal microbiota. however, the mode of action of this probiotic modification is still unclear. one possible explanation is the direct growth inhibiting or stimulating effect of the probiotic on other indigenous bacteria. therefore, the aim of the present study was to examine the growth interactions of the probiotic with different indigenous porcine bacteria in vitro. reference strain ... | 2015 | 25519527 |
| exopolysaccharide-producing probiotic lactobacilli reduce serum cholesterol and modify enteric microbiota in apoe-deficient mice. | probiotic bacteria have been associated with a reduction in cardiovascular disease risk, a leading cause of death and disability. | 2014 | 25320181 |
| characterization of a bovine isolate lactobacillus mucosae dpc 6426 which produces an exopolysaccharide composed predominantly of mannose residues. | to characterize lactobacillus strains with eps-producing ability compared with non-eps-producing lactobacilli of the same species for technological performance including simulated gastrointestinal tract (git) conditions. | 2014 | 24833280 |
| fructose 6-phosphate phosphoketolase activity in wild-type strains of lactobacillus, isolated from the intestinal tract of pigs. | phosphoketolases are key enzymes of the phosphoketolase pathway of heterofermentative lactic acid bacteria, which include lactobacilli. in heterofermentative lactobacilli xylulose 5-phosphate phosphoketolase (x5ppk) is the main enzyme of the phosphoketolase pathway. however, activity of fructose 6-phosphate phosphoketolase (f6ppk) has always been considered absent in lactic acid bacteria. in this study, the f6ppk activity was detected in 24 porcine wild-type strains of lactobacillus reuteri and ... | 2012 | 23101386 |
| longitudinal analysis of the intestinal microbiota in persistently stunted young children in south india. | stunting or reduced linear growth is very prevalent in low-income countries. recent studies have demonstrated a causal relationship between alterations in the gut microbiome and moderate or severe acute malnutrition in children in these countries. however, there have been no primary longitudinal studies comparing the intestinal microbiota of persistently stunted children to that of non-stunted children in the same community. in this pilot study, we characterized gut microbial community compositi ... | 2016 | 27228122 |
| genome sequence of lactobacillus mucosae lm1, isolated from piglet feces. | lactobacillus mucosae lm1, isolated from stool samples of a healthy piglet, displays good in vitro mucin adhesion and antimicrobial activity against pathogenic bacteria. to elucidate its antimicrobial effects and to find its epithelial cell and mucin adhesion genes, the genomic sequence of l. mucosae lm1 was investigated. | 2012 | 22887668 |
| antigenotoxic activity of lactic acid bacteria, prebiotics, and products of their fermentation against selected mutagens. | dietary components such as lactic acid bacteria (lab) and prebiotics can modulate the intestinal microbiota and are thought to be involved in the reduction of colorectal cancer risk. the presented study measured, using the comet assay, the antigenotoxic activity of both probiotic and non-probiotic lab, as well as some prebiotics and the end-products of their fermentation, against fecal water (fw). the production of short chain fatty acids by the bacteria was quantified using hplc. seven out of t ... | 2015 | 26404012 |
| effect of lactobacillus mucosae on in vitro rumen fermentation characteristics of dried brewers grain, methane production and bacterial diversity. | the effects of lactobacillus mucosae (l. mucosae), a potential direct fed microbial previously isolated from the rumen of korean native goat, on the rumen fermentation profile of brewers grain were evaluated. fermentation was conducted in serum bottles each containing 1% dry matter (dm) of the test substrate and either no l. mucosae (control), 1% 24 h broth culture of l. mucosae (t1), or 1% inoculation with the cell-free culture supernatant (t2). each serum bottle was filled anaerobically with 1 ... | 0 | 25358315 |
| acid production and growth by oral lactobacillus species in vitro. | to analyze the acid-producing and growth abilities of different oral lactobacillus species. | 2011 | 22298522 |
| Incorporating a mucosal environment in a dynamic gut model results in a more representative colonization by lactobacilli. | To avoid detrimental interactions with intestinal microbes, the human epithelium is covered with a protective mucus layer that traps host defence molecules. Microbial properties such as adhesion to mucus further result in a unique mucosal microbiota with a great potential to interact with the host. As mucosal microbes are difficult to study in vivo, we incorporated mucin-covered microcosms in a dynamic in vitro gut model, the simulator of the human intestinal microbial ecosystem (SHIME). We asse ... | 2012 | 21989255 |
| inhibitory effect of oral lactobacillus against oral pathogens. | aim: to determine the inhibitory effect of oral lactobacillus against putative oral pathogens. methods and results: total 357 strains comprising 10 species of oral lactobacillus, lactobacillus fermentum (195), lactobacillus salivarius (53), lactobacillus casei (20), lactobacillus gasseri (18), lactobacillus rhamnosus (14), lactobacillus paracasei (12), lactobacillus mucosae (12), lactobacillus oris (12), lactobacillus plantarum (11) and lactobacillus vaginalis (10) were used as producer strain ... | 2011 | 21801186 |
| (gtg)(5)-pcr fingerprinting of lactobacilli isolated from cervix of healthy women. | a group of lactobacilli isolated from the cervix of 31 healthy women was characterized by (gtg)(5)-polymerase chain reaction (pcr) fingerprinting in order to evaluate this method for identification of vaginal lactobacilli. obtained fingerprints were compared with profiles available in an in-house database of the ccm bacteria collection covering type and reference strains of multiple lactic acid bacteria including lactobacilli. selected strains representing individual clusters were further identi ... | 2011 | 21390569 |
| in vitro antagonistic activities of animal intestinal strains against swine-associated pathogens. | a wide range of enteropathogens cause costly diarrhoeal diseases in fattening piglets and account for food-related infections in humans. the objective of this study was to screen beneficial bacterial strains from the gastrointestinal tract of various animal sources for antagonistic activity against diverse pathogens associated with hazardous pig production times. using agar spot assays, 15 well-characterized strains belonging to lactobacillus, enterococcus, bifidobacterium and bacillus were stud ... | 2010 | 20226602 |
| drastic changes in fecal and mucosa-associated microbiota in adult patients with short bowel syndrome. | short bowel syndrome (sbs) is observed in humans after a large resection of gut. since the remnant colon and its associated microbiota play a major role in the outcome of patients with sbs, we studied the overall qualitative and quantitative microbiota composition of sbs adult patients compared to controls. the population was composed of 11 sbs type ii patients (with a jejuno-colonic anastomosis) and 8 controls without intestinal pathology. sbs patients had 38 +/- 30 cm remnant small bowel lengt ... | 2010 | 20172013 |
| ileal microbiota of growing pigs fed different dietary calcium phosphate levels and phytase content and subjected to ileal pectin infusion. | two experiments with growing pigs were conducted to determine the effects of dietary p and ca levels, phytase supplementation, and ileal pectin infusion on changes in bacterial populations in the ileum and on ileal and fecal fermentation patterns. growing pigs (bw 30.1 +/- 1.3 kg) were fitted with simple t-cannulas at the distal ileum and were fed a low-p corn-soybean meal control diet (3 g of p/kg), or the control diet supplemented with either 15 g of monocalcium phosphate (mcp)/kg (exp. 1) or ... | 2010 | 19820063 |
| characterization of intestinal bacteria tightly bound to the human ileal epithelium. | in order to perform selective isolation of bacteria tightly bound to the human gut, ileal biopsies of healthy volunteers were treated to wash out the mucus layer and loosely bound bacterial cells. rod-shaped anaerobic bacteria that had remained attached to the epithelial cells were isolated and identified at the species level. one isolate was identified as belonging to the bifidobacterium breve species, while all the others were lactobacilli of only two species, lactobacillus mucosae and lactoba ... | 2009 | 19782749 |
| beta-galactosidase, phospho-beta-galactosidase and phospho-beta-glucosidase activities in lactobacilli strains isolated from human faeces. | lactose intolerance, a serious health problem for asians, can be solved using probiotic bacteria having high lactose hydrolysis activities. we determined the distribution of beta-galactosidase (beta-gal), phospho-beta-galactosidase (p-betagal) and phospho-beta-glucosidase (p-beta-glc) activities in species of lactic acid bacteria (lab) isolated from human faeces to select strains for potential use in fermented dairy products, e.g. yogurt. | 2007 | 17944861 |
| carbohydrate-binding specificities of potential probiotic lactobacillus strains in porcine jejunal (ipec-j2) cells and porcine mucin. | bacterial lectins are carbohydrate-binding adhesins that recognize glycoreceptors in the gut mucus and epithelium of hosts. in this study, the contribution of lectin-like activities to adhesion of lactobacillus mucosae lm1 and lactobacillus johnsonii pf01, which were isolated from swine intestine, were compared to those of the commercial probiotic lactobacillus rhamnosus gg. both lm1 and pf01 strains have been reported to have good adhesion ability to crude intestinal mucus of pigs. to confirm t ... | 2016 | 27350617 |
| expression and characterisation of neopullulanase from lactobacillus mucosae. | to clone and express a neopullulanase gene from lactobacillus mucosae lm1 in escherichia coli and characterise the resulting recombinant neopullulanase. | 2016 | 27314479 |
| optimizing production of two potential probiotic lactobacilli strains isolated from piglet feces as feed additives for weaned piglets. | two probiotic strains, lactobacillus johnsonii x-1d-2 and lactobacillus mucosae x-4w-1, originally isolated from piglet feces, have been demonstrated to possess antimicrobial activities, antibiotic resistances and interleukin-6 induction ability in raw 267.4 macrophages in our previous study. these characteristics make l. johnsonii x-1d-2 and l. mucosae x-4w-1 good candidates for application in feed probiotics. in this study, soybeal meal, molasses and sodium acetate were selected to optimize th ... | 2015 | 26104525 |
| in vitro evaluation of the mucin-adhesion ability and probiotic potential of lactobacillus mucosae lm1. | in this report, we characterized the probiotic potential of lactobacillus mucosae lm1, focusing on its in vitro mucin-adhesion abilities. | 2014 | 24807045 |
| biofilm formation and ethanol inhibition by bacterial contaminants of biofuel fermentation. | bacterial contaminants can inhibit ethanol production in biofuel fermentations, and even result in stuck fermentations. contaminants may persist in production facilities by forming recalcitrant biofilms. a two-year longitudinal study was conducted of bacterial contaminants from a midwestern dry grind corn fuel ethanol facility. among eight sites sampled in the facility, the combined liquefaction stream and yeast propagation tank were consistently contaminated, leading to contamination of early f ... | 2015 | 26255598 |
| reduced-fat cheddar and swiss-type cheeses harboring exopolysaccharide-producing probiotic lactobacillus mucosae dpc 6426. | exopolysaccharide-producing lactobacillus mucosae dpc 6426 was previously shown to have promising hypocholesterolemic activity in the atherosclerosis-prone apolipoprotein-e-deficient (apoe(-/-)) murine model. the aim of this study was to investigate the suitability of reduced-fat cheddar and swiss-type cheeses as functional (carrier) foods for delivery of this probiotic strain. all cheeses were manufactured at pilot-scale (500-l vats) in triplicate, with standard commercially available starters: ... | 2015 | 26409971 |
| functional properties of lactobacillus mucosae strains isolated from brazilian goat milk. | the search for probiotic candidates among lactic acid bacteria (lab) isolated from food may uncover new strains with promising health and technological properties. lactobacillus mucosae strains attracted recent research attention due to their ability to adhere to intestinal mucus and to inhibit pathogens in the gastrointestinal tract, both related to a probiotic potential. properties of interest and safety aspects of three lb. mucosae strains (cnpc006, cnpc007, and cnpc009) isolated from goat mi ... | 2016 | 27943049 |
| enhanced butanol production from cassava with clostridium acetobutylicum by genome shuffling. | to obtain strains exhibiting high levels of solvent tolerance and butanol production, wild type strains of clostridium acetobutylicum butanol-producing strain gx01 and lactobacillus mucosae butanol-tolerant strain m26 were subjected to mutagenesis combining n-methyl-n-nitro-n-nitrosoguanidine induction with genome shuffling. after four successive rounds of genome shuffling, the c. acetobutylicum shuffled strain gs4-3 showing greater levels of fermentation performances (such as secreting a higher ... | 2016 | 26925615 |
| quantitative proteogenomics and the reconstruction of the metabolic pathway in lactobacillus mucosae lm1. | lactobacillus mucosae is a natural resident of the gastrointestinal tract of humans and animals and a potential probiotic bacterium. to understand the global protein expression profile and metabolic features of l. mucosae lm1 in the early stationary phase, the qexactive(tm) hybrid quadrupole-orbitrap mass spectrometer was used. characterization of the intracellular proteome identified 842 proteins, accounting for approximately 35% of the 2,404 protein-coding sequences in the complete genome of l ... | 2015 | 26761899 |
| bile tolerance and its effect on antibiotic susceptibility of probiotic lactobacillus candidates. | before use in practice, it is necessary to precisely identify and characterize a new probiotic candidate. eight animal lactobacilli and collection strain lactobacillus reuteri ccm 3625 were studied from the point of saccharide fermentation profiles, bile salt resistance, antibiogram profiles, and influence of bile on sensitivity to antibiotics. studied lactobacilli differed in their sugar fermentation ability determined by api 50chl and their identification based on these profiles did not corres ... | 2015 | 25413644 |
| an adhesin-like protein, lam29, from lactobacillus mucosae me-340 binds to histone h3 and blood group antigens in human colonic mucus. | a cell-surface 29-kda protein (lam29, cysteine-binding protein of the abc transporter) from lactobacillus mucosae me-340 showed an adhesin-like property for human abo blood group antigens expressed on the gastrointestinal mucosa. in addition, here we report that lam29 also bound to an 18-kda protein on human colonic mucus. by ligand blot assay and n-terminal amino acid sequence of the protein, it was identified as human histone h3. by ligand blot and microplate binding assays with recombinant hi ... | 2012 | 22972326 |
| analysis of gut microbiota in patients with parkinson's disease. | gut microbiota of patients with parkinson's disease and healthy volunteers was analyzed by the method of high throughput 16s rrna sequencing of bacterial genomes. in patients with parkinson's diseases, changes in the content of 9 genera and 15 species of microorganisms were revealed: reduced content of dorea, bacteroides, prevotella, faecalibacterium, bacteroides massiliensis, stoquefichus massiliensis, bacteroides coprocola, blautia glucerasea, dorea longicatena, bacteroides dorei, bacteroides ... | 2017 | 28429209 |
| a novel strain of lactobacillus mucosae isolated from a gaotian villager improves in vitro and in vivo antioxidant as well as biological properties in d-galactose-induced aging mice. | twelve isolates isolated from the gastrointestinal tracts of gaotian villagers in china, who had a lifespan of 92 yr, were examined for their antioxidants using free radical scavenging activity and 2,2-diphenyl-1-picrylhydrazyl. three strains (i.e., lactobacillus mucosae lmu1001, and lactobacillus plantarum lpl0902 and lpl0302) were selected as candidates to prepare yogurt for testing their antioxidants in a model of d-galactose-induced aging mice, with vitamin c as a positive control. the resul ... | 2016 | 26709186 |
| draft genome sequence of the lactobacillus mucosae strain marseille. | lactobacillus mucosae strain marseille, isolated from stool samples of a child suffering from a malnutrition disorder called kwashiorkor, produces bacteriocin and seems to have specific carbohydrate and lipid metabolisms different from those of other lactobacillus organisms. the draft genome sequence of this strain is presented here. | 2015 | 26227603 |
| selection of potential probiotic lactobacillus with inhibitory activity against salmonella and fecal coliform bacteria. | three hundred and sixty presumptive lactic acid bacteria (lab) isolated from pregnant sows, newborn, suckling, and weaned piglets were preliminarily screened for anti-salmonella activity. fifty-eight isolates consisting of lactobacillus reuteri (n = 32), lactobacillus salivarius (n = 10), lactobacillus mucosae (n = 8), lactobacillus johnsonii (n = 5), and lactobacillus crispatus (n = 3) were selected and further characterized for probiotic properties including production of antimicrobial substan ... | 2017 | 28712023 |
| increased propionate concentration in lactobacillus mucosae fermented wet brewers grains and during in vitro rumen fermentation. | this study was conducted to isolate and identify propionate-producing bacteria that can be used as an inoculum in improving wet brewers grains and rumen fermentation via increasing propionate concentration. | 2017 | 28425572 |