| suppression of phytohemagglutinin response by fungi from a "leukemia" house. | fungal isolates from the house of a husband and wife who both developed acute myelomonocytic leukemia were assayed for effects on the in vivo response to phytohemagglutinin in guinea pigs. skin responses to intradermal phytohemagglutinin were measured following injections of sterile fungal extracts. isolates of penicillium canescens, curvularia, fusarium sambucinum, fusarium equiseti and trichoderma koningii from the leukemia-associated house depressed the responses to phytohemagglutinin, but no ... | 1979 | 291385 |
| affinity chromatography of the cellulase system of trichoderma koningii. | a procedure involving affinity chromatography on cellulose was developed for separating enzymic components of the cellulase complex. cellobiase, carboxymethylcellulase, component c2 and cellobiohydrolase are adsorbed with increasing tenacity, and released, as highly purified components, as the ionic strength of the eluent is decreased. | 1978 | 417722 |
| the cellulase of trichoderma koningii. purification and properties of some endoglucanase components with special reference to their action on cellulose when acting alone and in synergism with the cellobiohydrolase. | 1. four principal endoglucanase components of trichoderma koningii cellulase were separated and purified by gel filtration on sephadex g-75, ion-exchange chromatography on deae- and sulphoethyl-sephadex and isoelectric focusing. 2. all four endoglucanases hydrolysed cm-cellulose, h3po4-swollen cellulose, cellotetraose and cellopentaose, but differed in the rate and mode of attack. 3. attack on cotton fibre by the endoglucanases was minimal, but resulted in changes that were manifested by an incr ... | 1978 | 417727 |
| characterization of a cellulase producing mold, trichoderma sp. w-10 isolated from infected mushroom spawn. | a potent cellulase producing mold, tichoderma sp. w-10 suitable for enzyme production with solid state culture was isolated from infected mushroom spawn, its characteristics and taxonomic studies were carried out. as a result, this mold was identified as trichoderma koningii (oudemans) according to the keys of gilman and rifai's classification system and the morphological features described by komatsu. meanwhile, a detail survey of physiological, cultural and biochemical characteristics were als ... | 1979 | 583274 |
| [chemical study of "penicillium" and "trichoderma" pigments (author's transl)]. | the green pigments from the fungi of the genera trichoderma and penicillium were partially extracted with formic acid. after two acid and one alkaline hydrolysis, the pigments of trichoderma viride and trichoderma koningii were sufficiently purified to undertake the chemical studies. the elemental and functional analyses, the u. v. and visible spectra and the chromatographic observations showed that these pigments are polyphenolic in nature. gel filtration on sephadex g50 suggested, on the one h ... | 1975 | 1171649 |
| [effect of various xylans on the biosynthesis of xylanases of fusarium moniliforme and trichoderma koningii]. | | 1975 | 1240581 |
| purification and characterization of an endoxylanase from trichoderma koningii g-39. | trichoderma koningii g-39 produced xylanases in submerged culture using oat spelt xylan or crystalline cellulose, avicel, as the sole carbon source. a low-mr xylanase was purified from the culture filtrate by ion-exchange chromatography on sp-trisacryl-m and gel filtration on fractogel tsk hw-50f. it was homogeneous on sds/page and isoelectric focusing. a typical procedure provided about 11-fold purification with 4.5% protein yield and 50% activity recovery. the purified enzyme has an mr value o ... | 1991 | 1910330 |
| two glyceraldehyde-3-phosphate dehydrogenase isozymes from the koningic acid (heptelidic acid) producer trichoderma koningii. | the sesquiterpene lactone koningic acid (heptelidic acid) irreversibly inactivated glyceraldehyde-3-phosphate dehydrogenase [d-glyceraldehyde 3-phosphate: nad+ oxidoreductase (phosphorylating)] (ec 1.2.1.12) (gapdh) and thus inhibits glycolysis. the koningic-acid-producing strain of trichoderma koningii m3947 was shown to contain the koningic-acid-resistant gapdh isozyme (gapdh i) under conditions of koningic acid production. in peptone-rich medium, however, no koningic acid production was obser ... | 1990 | 2226438 |
| the cellulase of penicillium pinophilum. synergism between enzyme components in solubilizing cellulose with special reference to the involvement of two immunologically distinct cellobiohydrolases. | two immunologically unrelated cellobiohydrolases (i and ii), isolated from the extracellular cellulase system elaborated by the fungus penicillum pinophilum, acted in synergism to solubilize the microcrystalline cellulose avicel; the ratio of the two enzymes for maximum rate of attack was approx. 1:1. a hypothesis to explain the phenomenon of synergism between two endwise-acting cellobiohydrolases is presented. it is suggested that the cellobiohydrolases may be two stereospecific enzymes concern ... | 1986 | 3707549 |
| specific inhibition of glyceraldehyde-3-phosphate dehydrogenase by koningic acid (heptelidic acid). | a sesquiterpene named koningic acid has been isolated from a strain of trichoderma koningii as a potent inhibitor of atp generation in the glycolytic pathway. from experiments with both cultured mouse carcinoma fm3a cells and isolated enzymes, it was shown that koningic acid is a specific inhibitor of glyceraldehyde 3-phosphate dehydrogenase that catalyzes the conversion of glyceraldehyde 3-phosphate to 3-phosphoglycerate. | 1985 | 4030504 |
| partial purification of ribonuclease from trichoderma koningii. | | 1974 | 4430034 |
| the purification and properties of the c 1 component of trichoderma koningii cellulase. | 1. the c(1) component that was isolated from a trichoderma koningii cellulase preparation (wood, 1968) by chromatography on deae-sephadex with a salt gradient was still associated with a trace of cm-cellulase activity (determined by reducing-sugar and viscometric methods). 2. further chromatography on deae-sephadex, with a ph gradient instead of a salt gradient, provided a c(1) component that could still produce reducing sugars from a solution of cm-cellulose (to a very limited extent), but whic ... | 1972 | 4674624 |
| the nature and mode of action of the cellulolytic component c1 of trichoderma koningii on native cellulose. | 1. a purified cellulolytic component c(1) was isolated free from associated activities of the cellulase complex and shown to act as a beta-1,4-glucan cellobiohydrolase on both simple and complex forms of native cellulose. 2. the enzyme releases terminal cellobiose units from cellulose, its extent of action being determined principally by the product and by the nature of the substrate. 3. component c(x) of the cellulase system is not required for the action of component c(1) (cellobiohydrolase). ... | 1973 | 4798312 |
| interactions between components of the cellulase complex of trichoderma koningii on native substrates. | | 1971 | 5107227 |
| the cellulase of fusarium solani. resolution of the enzyme complex. | 1. culture filtrates from fusarium solani were fractionated by ion-exchange chromatography on deae-sephadex, followed by gel chromatography on sephadex g-100, into a c(1) component, a c(x) component (cm-cellulase) and a beta-glucosidase (cellobiase) component. 2. the individual components showed little capacity for the solubilization of cotton fibre (cellulase activity), but when recombined in their original proportions 81% of the original cellulase activity was recovered. 3. the c(1) components ... | 1969 | 5389535 |
| the formation of short fibres from native cellulose by components of trichoderma koningii cellulase. | cellulolytic enzyme components of culture filtrates of trichoderma koningii were fractionated on ionic and non-ionic forms of sephadex and on cellulose powder (whatman) and examined for their ability to hydrolyse soluble carboxymethyl-cellulose, and to saccharify, solubilize and form short fibres from native undegraded cellulose of the type found in cotton. deae-sephadex provided two cm-cellulase components and a c(1) component; the c(1) component acted weakly and solely on cotton, forming solub ... | 1970 | 5460790 |
| cellulolytic enzyme system of trichoderma koningii. separation of components attacking native cotton. | 1. cell-free culture filtrates from trichoderma koningii were concentrated by precipitation with ammonium sulphate between the limits of 20% and 80% saturation. 2. removal of a low-molecular-weight carboxymethylcellulase (cm-cellulase) component by chromatography on sephadex g-75 had no effect on the ability of the enzyme complex to solubilize cotton. 3. further chromatography on deae-sephadex separated a component (c(1)) from the c(x) (cm-cellulase) and beta-glucosidase activities. separately t ... | 1968 | 5692804 |
| solubilization of native and derived forms of cellulose by cell-free microbial enzymes. | 1. cell-free enzymes from myrothecium verrucaria and trichoderma koningii hydrolyse native undegraded cellulose, as found in cotton fibres, in a random manner to short insoluble fibres and to minor amounts of soluble products. 2. enzyme preparations from m. verrucaria fail to attack the short fibres whereas preparations from t. koningii solubilize them completely to sugars at an optimum ph4.2-4.6. 3. the mode of hydrolysis of cotton cellulose by preparations from t. koningii involves from the ea ... | 1966 | 5968532 |
| affinity chromatography of proteolytic enzymes on silica-based biospecific sorbents. | new biospecific sorbents for affinity chromatography of proteolytic enzymes were prepared by the attachment of the cyclopeptide antibiotics bacitracin, bacilliquin or gramicidin s to aminosilochrom via a reaction with p-benzoquinone. the content of the cyclopeptide ligands within the sorbents varied from 2 to 46 mumol/g. the sorbents prepared by this reaction were successfully applied in the purification of the carboxylic proteinases produced by fungi, russula decolorans (a basidiomycete) and tr ... | 1981 | 6271864 |
| cellulolytic enzymes of trichoderma koningii. | | 1980 | 6776043 |
| [isolation and comparative properties of serine proteinases of the microscopic fungi trichoderma lignorum and trichoderma koningii]. | using affinity chromatography on bacitracin-sepharose combined with ion-exchange chromatography on aminosilochrome and isoelectrofocusing, individual serine proteases have been isolated for the first time from surface cultures of t. lignorum and t. koningii. the proteinases have pi values at 6.8 and 6.7 and ph optima of 10.5. both proteinases are stable within the ph range of 4-11 and have molecular weight of 21 000. the amino acid composition of t. lignorum enzyme is lys3his4arg9asx23thr17ser23 ... | 1981 | 7032610 |
| [purification and properties of low molecular weight endoglucanase of the cellulase complex from trichoderma koningii]. | a homogenous low molecular weight 1,4-beta-glucan glucanohydrolase (endoglucanase) has been isolated from a crude commercial preparation of cellokoningine p10x of t. koningii origin. the molecular weight of the enzyme as determined by polyacrylamide gel electrophoresis is 13 000. the endoglucanase was obtained as a lyophylized preparation free of the cellobiase activity. it was shown that cellobiose or methylcellobioside activate the effect of the low molecular weight endoglucanase (measured by ... | 1980 | 7189672 |
| [detection of transglycosylation reactions under the influence of trichoderma koningii and trichoderma viride cellulase]. | the transglycosylation reactions catalyzed by the low molecular weight endo-beta-1,4-glucanase from the cellulase complex of trichoderma koningii as well as unseparated complexes from tr. koningii and tr. viride were investigated. the products obtained from the mixture of cellodextrine (donor, 0.5%) and p-nitrophenyl-beta-d-glucopyranoside (acceptor, 0.4%) with the aid of the above enzymes were analyzed. transglycosylation products were separated by sephadex g-15 gel-filtration. in all the three ... | 1981 | 7197022 |
| the action on cellulose and its derivatives of a purified 1,4-beta-glucanase from trichoderma koningii. | the specific properties have been examined of the 1,4-beta-glucanase component of trichoderma koningii that participates in an early and effective stage of random breakdown of native cellulose to short fibres. the enzyme was purified and freed from associated components of the cellulase complex (particularly beta-glucosidase) that interfere with, and complicate interpretation of, the action of such enzymes. purification increased the specific activity 25-fold over culture filtrates; the enzyme h ... | 1981 | 7200359 |
| koninginin c: a biologically active natural product from trichoderma koningii. | koninginin c, a congener of koninginins a and b, was isolated from trichoderma koningii fermented on a shredded wheat medium. the compound inhibited the growth of etiolated wheat coleoptiles by 100% at 10(-3) m. it was a fine, white crystalline substance with a molecular formula of c16h28o4 and a melting point of 70-72 degrees c. | 1995 | 7612999 |
| trichokonin vi, a new ca2+ channel agonist in bullfrog cardiac myocytes. | the effects of trichokonin vi (= gliodeliquescin a), a peptaibol isolated from the culture broth of trichoderma koningii oudemans, on l-type ca2+ channel currents in single bullfrog atrial cells were investigated. our results showed that trichokonin vi is a new potent agonist of l-type ca2+ channels in cardiac membranes. | 1994 | 7698190 |
| studies on metabolites of mycoparasitic fungi. ii. metabolites of trichoderma koningii. | four peptaibols, named trichokonins (tks) v, vi, vii, and viii, were isolated from the culture broth of trichoderma koningii oudemans. primary structures of these peptaibols were elucidated by electrospray ionization mass spectrometry (esi-ms), fab-ms, and collision-induced dissociation (cid) techniques along with nuclear overhauser enhancement spectroscopy (noesy). | 1995 | 7728928 |
| molecular cloning and sequence analysis of the cellobiohydrolase i gene from trichoderma koningii g-39. | the cellobiohydrolase i gene, cbh1, has been cloned from an enhanced cellulase-producing strain, trichoderma koningii g-39. sequence comparisons show that t. koningii cbh1 is identical to that of t. reesei with the exception of 6 bp--two causing silent substitutions in the coding region, three differing in one of the introns, and one in 5'-noncoding region. thus, it should encode an identical cbhi to that of t. reesei despite the differences in morphological characters of the two species. analys ... | 1994 | 7764306 |
| extrinsic allergic alveolitis and asthma in a sawmill worker: case report and review of the literature. | a 34 year old sawmill maintenance engineer developed a dry cough that was associated with widespread wheezes and crackles in his lungs. his symptoms worsened, with work related lethargy, fever, and breathlessness, and the loss of a stone in weight. at that time, while still at work, he had a neutrophil leucocytosis and increased concentration of gamma globulins. when seen subsequently some two months after stopping work, his chest radiograph and lung function tests were normal, but the cells rec ... | 1994 | 8130843 |
| respiratory symptoms, immunological responses, and aeroallergen concentrations at a sawmill. | after identification of a case of extrinsic allergic alveolitis due to exposure to wood dust at a sawmill, all employees at the sawmill where he worked were studied with an occupational, environmental, and symptom questionnaire, spirometry, skin prick tests, and serum specific igg measurements. ninety five of current and 14 of 17 ex-sawmill workers were studied. as a basis for comparison, a group of 58 workers from a nearby light engineering factory were also studied. few women (6) were employed ... | 1994 | 8130844 |
| antifungal metabolites from trichoderma harzianum. | a detailed examination of the metabolites produced in liquid cultures by a strain of trichoderma harzianum, isolated from wheat roots, has resulted in the identification of a further five metabolites. two of these, cyclonerodiol [5] and the octaketide keto diol 6, have previously been isolated from a strain of trichoderma koningii. the structures of the three new octaketide-derived compounds 7, 8, and 10 have been deduced from spectroscopic and chemical studies. all newly isolated compounds show ... | 1993 | 8277317 |
| cloning of two isozymes of trichoderma koningii glyceraldehyde-3-phosphate dehydrogenase with different sensitivity to koningic acid. | koningic acid inhibits glyceraldehyde-3-phosphate dehydrogenase (gapdh) by binding to the sh group in the active center. the fungus trichoderma koningii, the producer of koningic acid, contains two gapdh isozymes (gapdhs i and ii). gapdh i is inhibited 50% by 1.1.10(-3) m koningic acid, while gapdh ii is inhibited 50% at 6.8 x 10(-6) m. cdnas of the two isozymes were cloned from t. koningii and their nucleotide sequences were determined. the sequence of coding region and codon usage in both clon ... | 1993 | 8439569 |
| studies on metabolites of mycoparasitic fungi. iv. minor peptaibols of trichoderma koningii. | three minor peptaibols, trichokonins (tks)-ia, ib, and ix, were obtained from the culture broth of trichoderma koningii oudemans. primary structures of these peptaibols were elucidated by ion-spray ionization mass spectrometry (isi-ms) including the collision-induced dissociation (cid) technique together with two-dimensional nuclear overhauser enhancement spectroscopy (noesy). | 1995 | 8536339 |
| on an unstable antifungal metabolite from trichoderma koningii. isolation and structure elucidation of a new cyclopentenone derivative (3-dimethylamino-5-hydroxy-5-vinyl-2-cyclopenten-1-one). | | 1996 | 8621363 |
| sensitivity of fungi to nikkomycin z. | nikkomycin z inhibited colony radial extension (kr) and hyphal density in geotrichum candidum, trichoderma koningii, mucor plumbeus, and fusarium oxysporum, although the level of inhibition varied with the fungal species and was influenced by the concentration of nutrients in the culture medium. while g. candidum and m. plumbeus were sensitive to the inhibitory effects of nikkomycin z, t. koningii and f. oxysporum were relatively tolerant of concentrations of the antibiotic up to 100 mumol. nikk ... | 1996 | 8634944 |
| studies on metabolites of mycoparasitic fungi. v. ion-spray ionization mass spectrometric analysis of trichokonin-ii, a peptaibol mixture obtained from the culture broth of trichoderma koningii. | the sequence of a peptide, trichokonin-ii (tk-ii), obtained from the culture broth of trichoderma koningii oudemans, was examined by ion-spray ionization mass spectrometry (isi-ms), including the collision-induced dissociation (cid) technique. tk-ii was concluded to be a mixture of three peptaibols, tk-iia, tk-iib, and tk-iic. | 1996 | 8882456 |
| fungal microflora biodiversity as a function of pollution in oued sebou (morocco). | an inventory of the fungal microflora present in sediments collected at 11 sites along oued sebou has been established. the influence of some climatic parameters (temperature, rainfall) and of pollution on the composition of the fungal communities has been questioned. emericella nidulans, eurotium amstelodami, neosartotya fisheri var. glabrum (ascomycetes), dreschslera biseptata (dematiaceae), penicillium citrinum (mucedinaceae) and phoma sp. (sphaeropsidale) can be considered as strains sensiti ... | 1997 | 9253167 |
| bioaccumulation of mycotoxins by shellfish: contamination of mussels by metabolites of a trichoderma koningii strain isolated in the marine environment. | to determine whether toxic metabolites produced by fungi could cause shellfish toxicities, mussels were contaminated in laboratory conditions by sterile filtrates of a liquid culture of a strain of the fungus trichoderma koningii previously isolated from a shellfish, the cockle (cerastoderma edule). mussels were kept in aerated natural seawater and fed with a culture of the microalga isochrysis galbana, to which a filtrate of liquid fungal culture was added. mussels were exposed to contamination ... | 1999 | 9920482 |
| total synthesis and membrane modifying properties of the lipopeptaibol trikoningin kb ii and its analogues with acyl chains of different length at the n- and c-termini. | trikoningin kb ii, a ten-amino acid residue lipopeptaibol blocked at the n-terminus by the n-octanoyl group and at the c-terminus by the 1,2-amino alcohol l-leucinol, and extracted from the fungus trichoderma koningii, exhibits membrane-modifying properties. we have synthesized by solution-phase methods trikoningin kb ii and several analogues with acyl chains of different length at the n- and c-termini. permeability measurements showed that an appropriate length of the linear acyl chain is a mor ... | 1999 | 10100125 |
| the abfb gene encoding the major alpha-l-arabinofuranosidase of aspergillus nidulans: nucleotide sequence, regulation and construction of a disrupted strain. | using a dna fragment containing the aspergillus niger abfb gene as a probe, the homologous aspergillus nidulans gene, designated abfb, has been cloned from a genomic library containing size-selected hindiii fragments. the nucleotide sequence of the a. nidulans abfb gene shows strong homology with the a. niger abfb, trichoderma reesei abf-1 and trichoderma koningii alpha-l-arabinofuranosidase/beta-xylosidase genes. regulation of abfb expression has been investigated in cultures induced with l-ara ... | 1999 | 10217508 |
| biocontrol of rhizoctonia solani and pythium ultimum on capsicum by trichoderma koningii in potting medium. | two isolates of trichoderma koningii were evaluated for efficacy in control of damping-off diseases in seedlings of capsicum annuum grown in pasteurized potting medium in a glasshouse. a selected isolate of binucleate rhizoctonia and two fungicides were also included as standards for control of rhizoctonia solani and pythium ultimum var. sporangiiferum. both isolates of t. koningii reduced seedling death caused by r. solani in one of two experiments, and by p. u. sporangii-ferum in two of three ... | 1999 | 10522381 |
| mechanistic study of beta-xylosidase from trichoderma koningii g-39. | the catalytic mechanism of the beta-xylosidase purified from the culture filtrate of trichoderma koningii g-39 was investigated. by nmr spectroscopy, the stereochemistry of the enzyme catalyzing the hydrolysis of 2,4-dinitrophenyl and p-nitrophenyl-beta-d-xylosides was found unequivocally to involve retention of the anomeric configuration. based on the k(cat) values of a series of arylxylosides with leaving group pk(a)s in the range of 4-10, an extended bronsted plot was constructed with a slope ... | 2000 | 10731699 |
| effective induction, purification and characterization of trichoderma koningii g-39 beta-xylosidase with high transferase activity. | a beta-xylosidase was induced and purified from the culture filtrate of trichoderma koningii g-39, grown in a medium containing 1% oat spelts xylan and 0.1% xylose. the presence of xylose unequivocally enhanced the induction of beta-xylosidase. the purified enzyme, which exhibited a significant alpha-arabinosidase activity, was obtained with high yield simply via ethanol precipitation and a single anion-exchange chromatography and was characterized as a monomeric glycoprotein with an estimated m ... | 2000 | 10744957 |
| physiological aspects of fungi isolated from root nodules of faba bean (vicia faba l.). | the present study was made to isolate and assess some physiological characteristics of root nodule-colonizing fungi. during this study, 17 fungal species were isolated from root nodule samples taken from faba bean plants (vicia faba l.) collected from different sites at assiut area (egypt). the growth of faba bean plants in pots was significantly promoted by soil inoculation with most fungi. growth was checked in pots with inocula of cladosporium cladosporioides, fusarium moniliforme, f: oxyspor ... | 2000 | 10772156 |
| effective extraction and purification of beta-xylosidase from trichoderma koningii fermentation culture by aqueous two-phase partitioning. | effective extraction of protein from bulk medium is an important technique in bioresearch. in the present study, we describe an extracellular beta-xylosidase from the fermentation supernatant of trichoderma koningii g-39 that was successfully extracted and purified simultaneously in a single step by using an aqueous two-phase partitioning method. this two-phase system was prepared by dissolving suitable amount of poly(ethylene glycol) (peg) and sodium dihydrogenphosphate (nah(2)po(4)) in aqueous ... | 2001 | 11166812 |
| trichoderma koningii as a biomineralizing fungous agent of calcium oxalate crystals in typical argiudolls of the los padres lake natural reserve (buenos aires, argentina). | the aim of the present study, performed on typical argiudolls in a natural reserve with little or no anthropic impact, was to characterize the fungous biomineralizing process of calcium oxalate crystals in organic horizons of the soil. the chosen sites possessed different plant cover, identified as acacia woods and grassy meadows with particular micro environmental conditions that have differing effects in the process of biomineralization. the contribution of the plant material in the soil is a ... | 2001 | 11572450 |
| [effect of enhanced uv-b radiation on chemical composition, fungal colonization and decomposition of spring wheat plant]. | under uv-b radiation, the cho (soluble carbohydrate) concentration in leaves decreased significantly, while the concentrations of hcel (holocellulose) and protein in leaves and that of hcel in stems increased significantly. hcel concentration in roots did not change significantly. under uv-b radiation, the colonization rates of penicillium ochro-chloron and aspergillus niger on leaves and stems increased significantly during growing phase, those of trichoderma koningii and aureobasidium pullulan ... | 2001 | 11757366 |
| combined use of lc/ms and a biological test for rapid identification of marine mycotoxins produced by trichoderma koningii. | trichoderma koningii oudemans, a strain isolated from a shellfish farming area, was selected for its high frequency in samples and its ability to produce metabolites when cultured in natural seawater. combined use of lc/ms and a biological test on blowfly larvae allowed the characterization of four compounds after purification in only two steps (vlc and hplc). esi/ms, a powerful tool for rapid identification and sequence determination of peptides, confirmed that these compounds were peptide, alp ... | 2002 | 11777568 |
| soil saprotrophic microfungi associated with roots of calamagrostis epigeios on an abandoned deposit of toxic waste from smelter factory processing pyrite raw materials. | saprotrophic microfungi associated with roots of calamagrostis epigeios growing in industrial waste contaminated with high concentrations of heavy metals (manganese, iron, zinc) were isolated on three different media. the isolates were obtained from three plots colonized by plant cenoses, representing three different succession series of plant cover: initial, intermediate, and advanced. a total of 60 species of saprotrophic microfungi were found in the roots during three samplings. in addition, ... | 2001 | 12032621 |
| determination of the stereochemistry of (-)-koninginin a by an x-ray analysis of its synthetic sample. | the absolute configuration of (-)-koninginin a [10-hexyl-11, 12-dioxatricyclo[7.2. 1.0<1 ,6>]dodecane-2,5-diol (1)], the antibiotic metabolite of trichoderma koningii and t. harzianum, was determined as 1s, 2r, 5s, 6s, 9s, 10s by an x-ray crystallographic analysis of its synthetic sample coupled with the established stereochemical outcome of sharpless asymmetric dihydroxylation used as the key reaction to prepare intermediate 4. | 2002 | 12353648 |
| [selection of beta-glucanase-producing trichoderma köningii t199 and its fermentation conditions]. | a mutant strain t199 producing about 8 times as much beta-glucanase as its parent strain trichoderma köningii t3 was obtained by treatment with ultraviolet light and n-methyl-n-nitro-n-nitrosoguanidine. fermentation was conducted in 250 ml flask, each containing 30 ml of medium consisted of 5% corn cob powder, 3% wheat bran and 1.4% nitrogen source no. 10 ((nh4)2so4 10%, peptone 20%, yeast extract 15%). the optima culture conditions were as below: initial ph 5.0, 30 degrees c, shaking speed 280 ... | 2001 | 12552835 |
| genetic and metabolic diversity of trichoderma: a case study on south-east asian isolates. | we have used isolates of trichoderma spp. collected in south-east asia, including taiwan and western indonesia, to assess the genetic and metabolic diversity of endemic species of trichoderma. ninety-six strains were isolated in total, and identified at the species level by analysis of morphological and biochemical characters (biolog system), and by sequence analysis of their internal transcribed spacer regions 1 and 2 (its1 and 2) of the rdna cluster, using ex-type strains and taxonomically est ... | 2003 | 12684020 |
| indications for the tracking of elevated nitrogen levels through the fungal route in a soil food web. | the objective of the present study was to determine the effects of elevated n in dead organic matter on the growth of fungi and to establish the consequences for the development of microbivores. therefore, three fungal species were cultured on scots pine litter differing in n content. the growth of the soil fungal species trichoderma koningii, penicillium glabrum and cladosporium cladosporioides was directly influenced by the n content (ranging from 1.25 to 2.19% n) of the substrate. for all thr ... | 2003 | 12927496 |
| hydrolysis of fibrous cotton and reprecipitated cellulose by cellulolytic enzymes from soil micro-organisms. | 1. the action of cell-free filtrates from trichoderma koningii was examined on undegraded cellulose in the form of cotton fibres, on degraded cellulose in the form of cellulose powder reprecipitated from phosphoric acid and on the soluble cellulose derivative cm-cellulose. 2. the cell-free filtrates compare favourably with intact cellulolytic micro-organisms in producing complete solubilization of undegraded as well as of degraded types of cellulose. enzymic solubilization of cotton fibres gives ... | 1965 | 14335814 |
| optimization of solid fermentation of cellulase from trichoderma koningii. | to exploit peashrub resources in ordos as fodders, it is very crucial to realize industrial production of cheap cellulase of high activity by optimizing culture technology, especially culture substrate. in this study, a new prescription experiment based on uniform design ideal was invented and successfully applied in the solid fermentation of trichoderma koningii f244, which was performed with two different temperature degrees. the activities of fpa, cotton lyase, cmcase and beta-glucosidase wer ... | 2004 | 15559819 |
| laboratory study of inocula production for composting processes. | six ligno-cellulolytic fungi were tested regarding to examine their capability to grow on agricultural wastes and produce inocula for composting. two residues were used: pepper plant wastes and almond shell residues. results showed the latter as the most adequate substrate for growth of fungi tested. on the contrary, trichoderma koningii, as well as hlc1 and hlc3, both fungi isolated from almond shell wastes, were able to persist in pepper plant wastes. modifications of aeration and ph significa ... | 2005 | 15607193 |
| mycodiversity on a dead stem of the giant hogweed--heracleum mantegazzianum sommer et levier. | during our investigations on microfungi in the ruhr area we were able to find the remarkable mycodiversity of different genera and species on dead stems of heracleum mantegazzianum, a neophyt for the european flora. altemaria alternata (fr.fr.) keissl., camarosporium spec., cladosporium herbarum (pers.) link, colletotrichum dematium (pers. ex fr.) gove, dactylella armandii yadv., dasyscyphus mollissimus (lasch.) dennis, dendryphion comosum wallr., endophragmia hyalosperma (corda) morgan-jones & ... | 2004 | 15756828 |
| process kinetics of inoculation composting of municipal solid waste. | a method was used to improve the composting efficiency by seeding with inoculum a (a blend of bacillus azotofixams, bacillus megaterium and bacillus mucilaginosus), inoculum b (a blend of effective cellulolytic strains, i.e. trichoderma koningii, streptomyces cellulosae, and white-rot fungi), and inoculum c (a mixture of inoculum a and inoculum b). there were four runs: the control run (not inoculated), run a, run b and run c. during the runs, parameters such as temperature, o2, co2 and h2s emis ... | 2005 | 15961224 |
| microbial transformation of silybin by trichoderma koningii. | microbial transformation of silybin a (1) and silybin b (2), the major hepatoprotective flavonolignan diastereomers from the fruits of silybum marianum, with the culture broth of trichoderma koningii gave two pairs of glucosylated derivatives. their structures were identified as silybin a 3-o-beta-d-glucopyranoside (3), silybin a 7-o-beta-d-glucopyranoside (4), silybin b 3-o-beta-d-glucopyranoside (5) and silybin b 7-o-beta-d-glucopyranoside (6) by spectroscopic methods. | 2006 | 16309908 |
| biochemical characterization of a beta-1,3-glucanase from trichoderma koningii induced by cell wall of rhizoctonia solani. | trichoderma species are readily isolated from brazilian cerrado soil by conventional methods and some of them were characterized as trichoderma koningii. the effect of carbon source on the production of beta-1,3-glucanases in the culture filtrates of a specific trichoderma koningii strain (all 13) was investigated. enzyme activity was detected in all carbon sources tested and only one band of beta-1,3-glucanase was detected in non-denaturing page. this enzyme was purified by sephacryl s-200 gel ... | 2006 | 16450064 |
| the effect of self-designed bifunctional rgd-containing fusion protein on the behavior of human keratinocytes and dermal fibroblasts. | in this study, self-designed bifunctional rgd-containing fusion protein (bfp) was grafted on the petri dish to evaluate its cytotoxicity and attachment efficiency on primary cultured keratinocytes and dermal fibroblasts. two lengths of the grgds sequences were separately fused to the n-terminus and c-terminus of the trichoderma koningii cellobiohydrolase i gene cellulose-binding domain, to serve as linking molecule between the cell and the substrate. the grafting procedure was no more labor-inte ... | 2006 | 16649172 |
| reidentification of cellulolytic enzyme-producing trichoderma strains w-10 and g-39. | strain w-10, originally identified as trichoderma koningii, and its supposed mutant g-39, published for production and gene expression of cellulase and xylanase, demonstrated morphological characteristics distinct from those of t. koningii, respectively. to clarify the identification derived from morphological characteristics, several methods were used, including electrophoretic karyotyping, internal transcribed spacer (its) analysis of rdna, and polymerase chain reaction (pcr) fingerprinting us ... | 2006 | 16788725 |
| broad-spectrum antimicrobial activity and high stability of trichokonins from trichoderma koningii smf2 against plant pathogens. | antimicrobial metabolites produced by trichoderma koningii smf2 exhibited antimicrobial activity against a range of gram-positive bacterial and fungal phytopathogens. purification of these metabolites was achieved using combinations of gel filtration and high-performance liquid chromatography. identified by liquid chromatography electrospray ionization tandem mass spectrometry, the active metabolites proved to be three known peptaibols: trichokonin vi, vii and viii. the trichokonins were stable ... | 2006 | 16790027 |
| beneficial effect of saprobe and arbuscular mycorrhizal fungi on growth of eucalyptus globulus co-cultured with glycine max in soil contaminated with heavy metals. | the effects of saprobe and arbuscular mycorrhizal (am) fungi on growth, chlorophyll and n, p and k content of eucalyptus globulus labill. growing in soil contaminated by heavy metals in the presence or absence of glycine max were investigated. glomus mosseae and glomus deserticola increased dry weight, shoot length, total n, p and k concentration and the quantity of chlorophyll in e. globulus shoots. the protection of eucalyptus by am fungi against the action of the heavy metals was more evident ... | 2007 | 16837125 |
| rapid and selective isolation of beta-xylosidase through an activity-based chemical approach. | beta-xylosidase is a key enzyme in the xylanolytic system with a great potential in many biotechnological applications, especially in the food as well as the pulp and paper industries. we have developed a chemical approach for the rapid screening and isolation of beta-xylosidase. activity probe lcl-6x targeting beta-xylosidase was utilized in this study. it carries a beta-xylopyranosyl recognition head, a latent trapping device consisting of a 2-fluoromethylphenoxyl group, and a biotin reporter ... | 2006 | 16892248 |
| mutagenesis and mechanistic study of a glycoside hydrolase family 54 alpha-l-arabinofuranosidase from trichoderma koningii. | a gh (glycoside hydrolase) family 54 alpha-l-arabinofuranosidase from trichoderma koningii g-39 (termed abf) was successfully expressed in pichia pastoris and purified to near homogeneity by cation-exchange chromatography. to determine the amino acid residues essential for the catalytic activity of abf, extensive mutagenesis of 24 conserved glutamate and aspartate residues was performed. among the mutants, d221n, e223q and d299n were found to decrease catalytic activity significantly. the kcat v ... | 2007 | 17002602 |
| degradation of cyanide by trichoderma mutants constructed by restriction enzyme mediated integration (remi). | remi technique was used to construct mutants with improved cyanide-degradation ability from biocontrol fungus trichoderma koningii strain t30. the plasmid pv2 transformation was confirmed by pcr and southern blot analysis. out of 21 transformants, 15 single-copied transformants (71.4%) were found. to compare enzyme activities of rhodanese and cyanide hydratase, t. atroviride t23, t. harzianum t21 and their transformants constructed by remi previously were also included. transformants tkb6 (0.173 ... | 2007 | 17112721 |
| tolerance of antarctic soil fungi to hydrocarbons. | little is known about the effects of hydrocarbons and fuel oil on antarctic filamentous fungi in the terrestrial antarctic environment. growth of fungi and bacteria from soils around rothera research station (adelaide island, antarctic peninsula) was assessed in the presence of ten separate aromatic and aliphatic hydrocarbons [marine gas oil (mgo), dodecane, hexadecane, benzoic acid, p-hydroxybenzoic acid, toluene, phenol, biphenyl, naphthalene and m- and p-xylenes with ethylbenzene]. aromatic h ... | 2007 | 17157897 |
| [fungus microbiota in air conditioners in intensive care units in teresina, piauí]. | with the aim of identifying the fungus microbiota in air conditioners in intensive care units (icus) within public and private hospitals in teresina, piauí, solid material was collected from ten different icus. thirty-three species of moniliaceae and dematiaceae were isolated, which was the first report of these in piauí. high frequencies of aspergillus niger van tieghem (60%), aspergillus fumigatus fres (50%), trichoderma koningii oudem (50%) and aspergillus flavus link: fr. (40%) were recorded ... | 2006 | 17308702 |
| optimization of cellulase complex formulation for peashrub biomass hydrolysis. | to improve efficiency and reduce cost, solid state simultaneous saccharification and fermentation of peashrub woody biomass was investigated under anaerobic conditions at 50 degrees c, with a cellulase-inoculant mixture consisting of trichoderma koningii cellulase, aspergillus niger cellulase, and lactobacillus. experimental formulations were prepared according to uniform prescription design principles. by crude protein, crude fiber models constructed using multivariate regression in spss and so ... | 2007 | 17347818 |
| operating characteristics of solid-state fermentation bioreactor with air pressure pulsation. | the development of solid state fermentation (ssf) technology is very important to the production of cellulase and ultimately to the utilization of natural cellulose. however, inadequate dissipation of heat generated by biological activities has prevented solid state fermentation from large-scale applications. the paper deals with the development of a novel ssf bioreactor with air pressure pulsation. by developing a measurement and control system under virtual instrument (vi) concept, performance ... | 2007 | 17476813 |
| [mycoparasites effect on reproductive ability of sclerotinia sclerotiorum sclerotia.]. | the ability to parasitise sclerotinia sclerotiorum and the effect on apothecia production was evaluated for the following antagonists: trichoderma harzianum; trichoderma koningii; gliocladium roseum and chaetomium globosum. plastic trays were filled with of steam-sterilized soil. each one of them was infested with sclerotia of s. sclerotiorum and the culture of the antagonists. the trays were kept in a greenhouse and after 30, 60 and 90 days, evaluations were made. the rates of carpogenic germin ... | 1998 | 17655415 |
| solid-state fermentation for trichokonins production from trichoderma koningii smf2 and preparative purification of trichokonin vi by a simple protocol. | trichokonins are peptaibols produced by trichoderma koningii smf2. the main isoforms are trichokonin vi, trichokonin vii and trichokonin viii. the solid-state fermentation (ssf) was applied for the production of trichokonin vi. the fermentation factors, which included inoculum size, incubation temperature, initial moisture content and initial ph, were investigated and optimized by response surface methodology. the maximum trichokonin vi production (4.07mg/g dry substrate) was achieved by employi ... | 2007 | 17669535 |
| diversity of microflora in the gut and casts of tropical composting earthworms reared on different substrates. | the diversity of fungi, bacteria, yeast, actinomycetes and protozoa were analysed in the gut and casts of eudrilus eugeniae, lampito mauritii, eisenia fetida and perionyx excavatus, both qualitatively and quantitatively as influenced by different feed substrates like clay loam soil, cowdung and pressmud. while actinomycetes (streptomyces albus, s. somaliensis, nocardia asteroides, n. caviae and saccharomonosporia) were not digested by any of these species of worms, protozoa (amoeba proteus, a. t ... | 2007 | 17717992 |
| koninginins, phospholipase a2 inhibitors from endophytic fungus trichoderma koningii. | many isolated compounds from endophytic fungus have been useful to human beings, mainly those with medicinal applications and particularly those that can be used in inflammatory processes. trichoderma fungi produce substances known as koninginins that have great structural similarity to compounds like flavonoids and vitamin e, which are able to inhibit the phospholipase a(2) (pla(2)). in this work, koninginins a, e and f (kona, kone and konf, respectivamente) isolated from trichoderma koningii h ... | 2008 | 17983638 |
| efficient biodegradation of cyanide and ferrocyanide by na-alginate beads immobilized with fungal cells of trichoderma koningii. | cyanide or metal cyanide contaminations have become serious environmental and food-health problems. a fungal mutant of trichoderma koningii, tka8, constructed by restriction enzyme-mediated integration, has been verified to have a high cyanide degradation ability in our previous study. in this study, the mutant cells were entrapped in sodium-alginate (na-alginate) immobilization beads to degrade cyanide and ferrocyanide in a liquid mineral medium. the results showed that the fungus in immobiliza ... | 2007 | 18026223 |
| characterization of a thermostable and acidic-tolerable beta-glucanase from aerobic fungi trichoderma koningii zju-t. | an extreme thermostable and acidic tolerable beta-glucanase was isolated and characterized from aerobic fungi trichoderma koningii zju-t. the optimal reaction temperature and ph for the beta-glucanase were 100 degrees c and ph 2.0, respectively. the beta-glucanase showed increased stability at higher temperatures and lower ph values when compared to other beta-glucanases. the optimum conditions for the beta-glucanase stability were found to be ph 4.0 and 80 degrees c. even subjected to 100 degre ... | 2007 | 18034703 |
| evaluation of the effects of chemical versus biological control on botrytis cinerea agent of gray mould disease of strawberry. | this study investigates on effects of four fungicide and six isolate from trichoderma and gliocladium on botrytis cinerea agent grey mold of strawberry under library and greenhouse condition. the effect of four fungicides i.e. benomyl, dichlofluanid, captan and triadimenol on b. cinerea was studied in the laboratory condition by method mixed poison to culture medium. it was shown that the fungicide including benomyl, triadimenol, dichlofluanid and captan were able to inhibit mycelial growth of b ... | 2007 | 18396812 |
| [filamentous fungi preservation in distilled water.]. | some methods for keeping the fungal culture collection have been used. however, the choice of either one on the basis that must ensure the cultural genetic stability and its phenotipic characteristics. in this work the preservation method in distilled water recognized in the literature as a single, economic and certain method that guarantie the survival of fungus cultures for long periods was used. 26 strains of generus and species: aspergillus niger, aspergillus candidus, fusarium sp., fusarium ... | 1998 | 18473541 |
| the trichoderma koningii aggregate species. | the morphological concept of trichoderma koningii is found to include several species that differ from each other in details of phenotype (including conidium morphology, growth rate) and biogeography. phylogenetic analysis utilizing partial sequences of the translation-elongation factor 1 alpha (tef1), as well as fragments of actin and calmodulin genes, indicate that phenotypic characters typical of t. koningii evolved independently in three well-separated main lineages. combined molecular and p ... | 2006 | 18490990 |
| synergism of endoenzyme and exoenzyme on hydrolysis of soluble cellulose derivatives. | a kinetic model that represents the reaction of hydrolysis of water-soluble cellulose derivatives by a mixed endo- and exoenzyme system is proposed with the following assumptions: at an early stage of the reaction, endoenzymes split the substrate molecule in order to supply the newly formed nonreducing ends to exoenzymes until the molecular weight of the substrates reaches a low value; after that point, the reaction kinetics obeys only the rate equation of the reaction of the exoenzymes in which ... | 1986 | 18555406 |
| soil chemical and physical properties associated with suppression of take-all of wheat by trichoderma koningii. | abstract trichoderma koningii, originally isolated from a take-all-suppressive soil in western australia, has been shown to protect wheat against take-all disease and increase grain yield in field trials in australia, china, and the united states. however, within a region, the level of protection provided by t. koningii can dramatically vary between field sites. we evaluated suppression of take-all by this fungus in eight silt loams from the pacific northwest of the united states and the influen ... | 1997 | 18945008 |
| synthesis and in vitro evaluation of the antifungal activities of dihydropyrimidinones. | copper (ii) chloride in the absence of any solvent, efficiently catalyses the synthesis of dihydropyrimidinones (80-96% yields) by the biginelli reaction. six compounds were selected and examined their antifungal activities against the radial growth of three fungal species viz., trichoderma hammatum, trichoderma koningii and aspergillus niger. | 2008 | 18977139 |
| [cloning and functional analysis of two regulators involved in regulation of cellulase formation in trichoderma koningii]. | acei and xyr1 are two regulators that potentially involve in regulation of cellulases and xylanases formation in trichoderma reesei, they compete for a binding site in the xyn1 (xylanase1-encoding) gene promoter. to further investigate the mechanism for the transcriptional regulation of cellulases, dna-binding domains of both acei and xyr1 in t. koningii were expressed from e. coli. they both showed bindings to the cbh1 promoter fragment (-304 bp to -18 bp) by electrophoresis mobility shift assa ... | 2008 | 19149196 |
| [analysis of differential protein expression of remi mutant of trichoderma koningii]. | on purpose of expounding the phenotype change of trichoderma mutant which produces cellulase, protein profiles of t. koningii cicc and its mutant tk-2r-1 were detected by 2-de. eight unique spots were found and four of them were identified and their functions by ms-tof-tof were connected with growth and metabolization of trichoderma spp. the research provides a new theoretical basis and methods for the construction of trichoderma mutant which is used to produce efficient cellulase. | 2009 | 19306692 |
| [study on conformation transitions of trichokonin vi, a peptaibol-like antimicrobial peptide, in different solvents by circular dichroism spectroscopy]. | trichokonin vi, a peptaibol-like antimicrobial peptides isolated from the cultured substrates of trichoderma koningii smf2, has 20 amino acid residues. the conformational flexibility of trichokonin vi in organic solvents with different polarities, aqueous solvents and membrane mimic solvents was studied by circular dichroism spectroscopy. trichokonin vi takes on a typical alpha-helical structure in different organic solvents, but helicity decreases in aqueous solvent. the helical content increas ... | 2010 | 20384145 |
| trichodermaketones a-d and 7-o-methylkoninginin d from the marine fungus trichoderma koningii. | five new polyketide derivatives, 7-o-methylkoninginin d (1) and trichodermaketones a-d (2-5), together with four known compounds, koninginins a, d, e, and f, were isolated from the marine-derived fungus trichoderma koningii. trichodermaketones a (2) and b (3) are unprecedented polyketides with a bistetrafuran-containing tricyclic skeleton. the chemical structures and absolute configurations of compounds 1-5 were elucidated by comparing with literature data and extensive spectroscopic methods, in ... | 2010 | 20384316 |
| a method of purification, identification and characterization of β-glucosidase from trichoderma koningii as3.2774. | in this study, we used native gradient-polyacrylamide gel electrophoresis and electroelution (nggee) to purify enzymatic proteins from trichoderma koningii as3.2774. with this method, we purified eight enzymatic proteins and classified them to the cellulase system by comparing secretions of t. koningii in inductive medium and in repressive medium. it resulted in 24-fold β-glucosidase (bg) purification with a recovery rate of 5.5%, and a specific activity of 994.6iumg(-1) protein. the final yield ... | 2010 | 20691221 |
| further evidence from the effect of fungi on breaking opuntia seed dormancy. | recently, we found that fungi are involved in breaking seed dormancy of opuntia streptacantha, and that the effect of fungi on seeds is species-specific. however, the effect of fungi on seed germination from other opuntia spp has not been evaluated. thus, we evaluated the effect of four fungal species (penicillium chrysogenum, phoma sp., trichoderma harzianum, trichoderma koningii) on the germination of opuntia leucotricha, an abundant species in the chihuahuan desert, mexico. we found that seed ... | 2010 | 20861689 |
| are fungi important for breaking seed dormancy in desert species? experimental evidence in opuntia streptacantha (cactaceae). | seeds of opuntia spp. have physiological dormancy; they need a period of after-ripening to break dormancy, and the embryos have low growth potential. we evaluated the combined effects of seed age and presence of fungi on the testa on germination of opuntia streptacantha, an abundant species in the chihuahuan desert (mexico), assuming that older seeds have broken seed dormancy and fungi can reduce mechanical resistance to germination. in a preliminary experiment, we found no germination of 9-year ... | 2011 | 21143736 |
| cunninghamella echinulata a new biosorbent of metal ions from polluted water in egypt. | thirty-two fungal species were isolated from a polluted watercourse near the talkha fertilizer plant, mansoura province, egypt. aspergillus niger, a. flavus, cunninghamella echinulata and trichoderma koningii were isolated frequently. on the basis of its frequency, cunninghamella echinulata was chosen for biosorption studies. free and immobilized biomass of c. echinulata sequestered ions in this decreasing sequence is: pb >cu >zn. the effects of biomass concentration, ph and time of contact were ... | 2004 | 21148940 |
| [antagonism of trichoderma spp. to fungi caused root rot of sophora tonkinensis]. | to study the antagonism of trichoderma spp. to fungi s9(fusarium solani)which caused root rot of sophora tonkinensis and discuss the further develop prospects of microbial biological control in soil-borne diseases on chinese herbal medicines. | 2011 | 21809533 |
| Evaluation of cellulases produced from four fungi cultured on furfural residues and microcrystalline cellulose. | Four fungal strains-Trichoderma viride, Aspergillus niger, Trichoderma koningii, and Trichoderma reesei-were selected for cellulase production using furfural residues and microcrystalline cellulose (MCC) as the substrates. The filter paper activity (FPA) of the supernatant from each fungus was measured, and the performance of the enzymes from different fungal strains was compared. Moreover, the individual activities of the three components of the cellulase system, i.e., ß-glucosidase, endoglucan ... | 2011 | 22116409 |
| isolation, identification and antimicrobial activity of propolis-associated fungi. | propolis is a natural product widely known for its medicinal properties. in this work, fungi present on propolis samples were isolated, identified and tested for the production of antimicrobial metabolites. twenty-two fungal isolates were obtained, some of which were identified as alternaria alternata, aspergillus flavus, bipolaris hawaiiensis, fusarium merismoides, lasiodiplodia theobromae, penicillium citrinum, penicillium crustosum, penicillium janthinellum, penicillium purpurogenum, pestalot ... | 2013 | 23439233 |
| fungal biodeterioration, aflatoxin contamination, and nutrient value of "suya spices". | this work aimed to analyze the nutrient values, examine the biodeteriorating fungi biota, and analyze the mycotoxin contents of "suya spices." fungi with highest percentage occurrence on all the samples are aspergillus niger, aspergillus flavus, aspergillus parasiticus, aspergillus ochraceus, fusarium sp., rhizopus stolonifer, yeast, and trichoderma koningii. nutrient composition of the samples is significantly different statistically (p < 0.05) with high protein (9.53% to 13.17%), fiber (9.27 t ... | 2016 | 27092289 |
| cellulose degradation and cellulase activity of five cellulolytic fungi. | biodegradation of pure cellulose powder, bagasse and wheatstraw by five cellulolytic fungi,aspergillus niger, chaetomium globosum, scopulariopsis brevicaulis, trichoderma koningii andtrichothecium roseum, was studied in solid culture conditions. minimum degradation was with pure cellulose. bagasse and wheatstraw were the most suitable for growth and activity of cellulolytic fungi. all fungi contained cellulase activity. | 1990 | 24429892 |
| bioconversion of straw into improved fodder: preliminary treatment of rice straw using mechanical, chemical and/or gamma irradiation. | crude protein (cp) content of mechanically ground rice straw into small particles by an electric grinder and reducing value (rv) and soluble protein (sp) in the culture filtrate were lower than that of the chopped straw into 5~6 cm lengths when both ground and chopped straws were fermented with aspergillus ochraceus, a. terreus or trichoderma koningii, at steady conditions. the reduction rate of rv, sp and cp was 22.2, 2.4, 7.3%; 9.1, 4.9, 8.5% or 0.0, 0.0, 3.6% for the three fungi, respectively ... | 2006 | 24039464 |
| bioconversion of straw into improved fodder: mycoprotein production and cellulolytic acivity of rice straw decomposing fungi. | sixty two out of the sixty four species of fungal isolates tested could produce both exo-β1,4-gluconase (c1) and endo-β1,4-gluconase (cx) on pure cellulose and rice straw as carbon source in czapek's medium. fifty-eight and fifteen species were able to grow at 25℃ and at 45℃, respectively. eleven species could grow at both 25℃ and 45℃ while, four species appeared only at 45℃. the most cellulolytic species at 25℃ was trichoderma koningii producing 1.164 c1 (mg glucose/1 ml culture filtrate/1 hr) ... | 2005 | 24049480 |
| hypocrol a, a new tyrosol derivative from a sponge-derived strain of the fungus hypocrea koningii. | in continuation of our search for new antibacterial and antioxidant metabolites from sponge-derived fungi, one new tyrosol derivative, hypocrol a (1), together with four known congeners, trichodenol b (2), 4-hydroxyphenethyl acetate (3), 4-hydroxyphenethyl tetradecanoate (4) and 1-oleyltyrosol (5), was isolated from the strain hypocrea koningii pf04. their planar structures were unequivocally elucidated by spectroscopic methods and comparison with the literature data. all the compounds displayed ... | 2016 | 26828743 |
| construction of transgenic trichoderma koningi with chit42 of metarhizium anisopliae and analysis of its activity against the asian corn borer. | the chit42 gene was cloned from metarhizium anisopliae cy1 and was inserted into trichoderma koningii t30 genome by protoplast transformation. sixteen transgenic isolates were identified by polymerase chain reaction analysis. the chit42 gene was 1275 bp in length and its coded protein was approximately 42 kda in size. semi-quantitative rt-pcr analysis and the measurement of the chitinase activity under induced conditions were conducted. mortality of the asian corn borer (ostrinia furnacalis) was ... | 2012 | 22560024 |