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influence of light on pectic enzymes in root exudates of trifolium alexandrinum inoculated with rhizobium trifolii.an in vitro experiment was conduced under bacteriologically controlled conditions to examine the effect of light on the production of pectin methyl esterase (pme) and pectin polygalacturonase (pg) in the root exudates of trifolium alexandrinum inoculated with an efficient strain of rhizobium trifolii. the results revealed that pme and pg increased with an increase in the duration of light to which plants were exposed. however, both the enzymes were detected in the root exudates of nonphotosynthe ...1978664934
isolation of streptomyces finlayi from the rhizoplane of egyptian clover (trifolium alexandrinum).a representative strain (531) of green streptomycetes occurring in the root surface region of egyptian clover (trifolium alexandrinum) was identified as a typical strain of streptomyces finlayi, a species which seems capable of living, in loose protocooperative or commensalistic association, also with soil invertebrates (szabó 1974). in the aerial mycelium of strain 531, spore-chains both with a typical hairy sheath and without a sheath (smooth spores) were observed. according to our present and ...1978735503
swelling forms on roots of berseem-clover (trifolium alexandrinum), caused by a strain of rhizobium trifolii. 19734801559
preliminary report on the hypoglycaemic effect of trifolium alexandrinum and lupinus termis in animal and man. 19695365916
[trifolium alexandrinum and persian clover, 2 "new" fodder plants]. 19666013190
reproductive performance and milk production of damascus goats fed acacia shrubs or berseem clover hay in north sinai, egypt.thirty-three adult damascus does (29 +/- 1.1 kg bw) were fed 0.6 kg per head per day of a concentrate diet plus ad libitum rice straw (oryza sativa, control, r), or green acacia (acacia saligna, a) or berseem clover hay (trifolium alexandrinum, b). all treatment groups had free access to underground saline water containing 3600 ppm tds. the dm intake was lower (p < 0.05) for the r group but did not differ between the other two groups. the dm intake of acacia was 0.64 kg per head per day (1.98% b ...200111234194
egyptian plant species as new ozone indicators.the aim of this study was to test and select one or more highly sensitive, specific and environmentally successful egyptian bioindicator plants for ozone (o3). for that purpose more than 30 egyptian species and cultivars were subjected to extensive screening studies under controlled environmental and pollutant exposure conditions to mimic the egyptian environmental conditions and o3 levels in urban and rural sites. four plant species were found to be more sensitive to o3 than the universally use ...200212395848
effect of sulfadiazine-contaminated pig manure on the abundances of genes and transcripts involved in nitrogen transformation in the root-rhizosphere complexes of maize and clover.the antibiotic sulfadiazine (sdz) can enter the environment by application of manure from antibiotic-treated animals to arable soil. because antibiotics are explicitly designed to target microorganisms, they likely affect microbes in the soil ecosystem, compromising important soil functions and disturbing processes in nutrient cycles. in a greenhouse experiment, we investigated the impact of sulfadiazine-contaminated pig manure on functional microbial communities involved in key processes of the ...201020971872
no-choice preference of cerotoma trifurcata (coleoptera: chrysomelidae) to potential host plants of bean pod mottle virus (comoviridae) in iowa.to better understand the naturally occurring host range of bean pod mottle virus (family comoviridae, genus comovirus, bpmv) and its principal vector cerotoma trifurcata (förster) (coleoptera: chrysomelidae), 18 field-collected perennial plant species were tested for the presence of bpmv. by using no-choice assays, we determined the preference of these plants by bean leaf beetle, by measuring their level of herbivory relative to soybean, glycine max (l.). new food hosts for adult bean leaf beetl ...200717598542
assessment of losses in honey yield due to the chalkbrood disease, with reference to the determination of its economic injury levels in egypt.in egypt, the chalk brood (chb) disease caused by the fungus, ascosphaera apis maassen started again infesting the honeybee, apis melifera l (hymenoptera: apidae) colonies after a cessation pause of seven to nine years. for the first time, an attempt has been made to look into the problem of the chb disease from the view point of assessing losses in both clover (trifolium alexandrinum) and chinus (chinus molus) honey yields. in this regard, two techniques were adopted. under the natural conditio ...200516628907
foliar chlorosis in symbiotic host and nonhost plants induced by rhizobium tropici type b strains.rhizobium tropici ciat899 induced chlorosis in the leaves of its symbiotic hosts, common bean (phaseolus vulgaris l.), siratro (macroptilium atropurpureum urb.), and leucaena leucocephala (lam.) de wit. chlorosis induction by strains ciat899 and ct9005, an exopolysaccharide-deficient mutant of ciat899, required carbon substrate. when the bacteria were added at planting in a solution of mannitol (50 g/liter), as few as 10 cells of ciat899 were sufficient to induce chlorosis in bean plants. all ca ...199316348994
isolation of pythium oligandrum from egyptian soil and its mycoparasitic effect on pythium ultimum var. ultimum the damping-off organism of wheat.pythium oligandrum drechsler bearing spherical sporangia with complex subglobose elements was isolated for the first time in egypt from agricultural field soil cultivated with alfalfa (trifolium alexandrinum) in el-minia, egypt. this fungus was found to be an active bio-control agent against p. ultimum var. ultimum, the damping-off organism of wheat. in agar plates, p. oligandrum parasitized p. ultimum var. ultimum hyphae with the aid of thin haustorial branches or infection pegs, eventually lea ...199716284719
effects of trifolium alexandrinum extracts on streptozotocin-induced diabetes in male rats.the effects of (water, hexane and ethanolic) extracts prepared from the flower head of trifolium alexandrinum (leguminosae) were examined in the treatment of diabetes induced experimentally by streptozotocin (stz) in rats. a single dose of stz (50 mg/kg) produced a hyperglycemia, decrease in insulin level and elevation in glycosylated hemoglobin of rbcs as well as elevated serum total lipids, triglycerides and cholesterol levels. also, a decreased serum high-density lipoprotein and in hepatic gl ...200415499220
the biflavonoid, amentoflavone degrades dna in the presence of copper ions.previous reports from this laboratory have shown that flavonoids including apigenin are capable of inducing oxidative dna cleavage in the presence of copper ions. in the present report, we have examined the ability of amentoflavone, a biflavonoid which is a dimer of apigenin, to catalyze the degradation of dna. amentoflavone was found to degrade calf thymus dna in the presence of cu(ii) at a rate almost twice that of apigenin. amentoflavone was also shown to reduce cu(ii) to cu(i) and to generat ...200415130600
interspecific hybridization of trifolium alexandrinum with t. constantinopolitanum using embryo rescue.the embryo rescue technique was successfully used to raise hybrids between trifolium alexandrinum and t. constantinopolitanum. as a result of its narrow genetic base, genetic improvement in egyptian clover (syn. berseem; t. alexandrinum), an important fodder crop in tropical and subtropical countries, is hampered, thereby making it imperative to introduce alien genes from related species. in a conventional interspecific hybridization program, hybrids could not be raised due to post-fertilization ...200414745504
influence of diet on growth yields of rumen micro-organisms in vitro and in vivo: influence on growth yield of variable carbon fluxes to fermentation products.the efficiency of rumen microbial production (emp) in vitro and in vivo was examined for three roughages (lucerne (medicago sativa l.) hay, oat (avenia sativa l.)-berseem clover (trifolium alexandrinum cultivar bigbee) hay and maize (zea mays l.) crop residue (mcr)) and for five isonitrogenous (106 g crude protein (nx6.25)/kg) diets formulated from lucerne hay, oat-berseem clover hay, mcr, soybean meal and maize grain to provide degradable intake protein for the production of 130 g microbial pro ...200313129469
improving the reproductive performance of egyptian buffalo cows by changing the management system.the objective of the present study was to determine the possibility to improve the reproductive performance of buffalo cows through the continuous exposure to bull with grazing and free-stall housing management. sixty-four egyptian multiparous buffalo cows raised under two different management systems in two farms were used in this study. the cows in the first farm (management system 1, ms1) were loose--housed in a free-stall yard, grazed for 4 h per day, suckled their calves for 2-3 months and ...200312535580
degradation pattern and risk assessment of chlorantraniliprole on berseem (trifolium alexandrinum l.) using high performance liquid chromatography.the persistence pattern of chlorantraniliprole on berseem leaves and its risk assessment for the safety of cattle were studied. quechers method was used for the extraction and cleanup of samples and the residues of chlorantraniliprole were estimated using high performance liquid chromatography (hplc) and confirmed by liquid chromatograph-mass spectrometer (lcms-ms). the dissipation studies on berseem were carried out by application of chlorantraniliprole at five different dosages i.e. 11.6, 17.1 ...201425048894
hormonal concentrations and reproductive performance of holstein heifers fed trifolium alexandrinum as a phytoestrogenic roughage.effects of phytoestrogen isoflavones in berseem clover on hormonal balance during early pregnancy and fertility of heifers were studied. holstein heifers (n=26) were divided into two equal homogenous groups. heifers in the first group (clover-fed group) were fed egyptian clover (trifolium alexandrinum) as oestrogenic roughage; whereas the heifers in the second group (silage-fed group) were fed maize silage. concentrations of four isoflavone aglycones (genistein. daidzein, biochanin a and formono ...201627137835
the symbiovar trifolii of rhizobium bangladeshense and rhizobium aegyptiacum sp. nov. nodulate trifolium alexandrinum in egypt.in the present work we analyzed the taxonomic status of several rhizobium strains isolated from trifolium alexandrinum l. nodules in egypt. the 16s rrna genes of these strains were identical to those of rhizobium bangladeshense blr175(t) and rhizobium binae blr195(t). however, the analyses of reca and atpd genes split the strains into two clusters. cluster ii strains are identified as r. bangladeshense with >98% similarity values in both genes. the cluster i strains are phylogenetically related ...201627236564
reproduction of meloidogyne incognita on winter cover crops used in cotton production.substantial reproduction of meloidogyne incognita on winter cover crops may lead to damaging populations in a subsequent cotton (gossypium hirsutum) crop. the amount of population increase during the winter depends on soil temperature and the host status of the cover crop. our objectives were to quantify m. incognita race 3 reproduction on rye (secale cereale) and several leguminous cover crops and to determine if these cover crops increase population densities of m. incognita and subsequent dam ...200619259434
lucerne transient streak virus; a recently detected virus infecting alfafa (medicago sativa) in central saudi arabia.a survey was conducted to determine the status of lucerne transient streak virus (ltsv) in three high-yielding alfalfa regions in central saudi arabia (riyadh, qassim, and hail) during 2014. three hundred and eight symptomatic alfalfa, and seven sonchus oleraceus samples were collected. das-elisa indicated that 59 of these samples were positive to ltsv. two isolates of ltsv from each region were selected for molecular studies. rt-pcr confirmed the presence of ltsv in the selected samples using a ...201728167887
saponins of trifolium spp. aerial parts as modulators of candida albicans virulence attributes.the aim was to provide the insight into the biology of c. albicans influenced by undescribed yet properties of saponin-rich (80%-98%) fractions (sapfs), isolated from extracts of trifolium alexandrinum, t. incarnatum, t. resupinatum var. resupinatum aerial parts. their concentrations below 0.5 mg/ml were arbitrarily considered as submics for c. albicans atcc 10231 and were further used. sapfs affected yeast enzymatic activity, lowered tolerance to the oxidative stress, to the osmotic stress and ...201425050858
morning versus afternoon cutting time of berseem clover (trifolium alexandrinum l.) affects feed intake, milk yield and composition in girgentana goats.twenty lactating girgentana goats were used to evaluate the effect of morning v. afternoon cutting time of berseem clover (trifolium alexandrinum l.) on feed intake, milk yield and milk composition. goats were randomly divided into two groups of ten animals, receiving 10 kg of fresh berseem clover cut at 9.00 (am group) or 16.00 (pm group), respectively; 500 g of concentrate was given individually to goats before offering forage. feed intake increased (p<0·01) in the pm group (30·5 v. 25·3 g dry ...201121939575
flowering catch crops used as forage plants for dairy cows: influence on fatty acids and tocopherols in milk.the effect of several flowering dicotyledonous catch crop plants (dicots) on milk fat quality in cows was investigated to test the hypothesis that their phenolic compounds may inhibit ruminal biohydrogenation and thus enhance the transfer to milk of intact, plant-derived polyunsaturated fatty acids. berseem clover (trifolium alexandrinum), buckwheat (fagopyrum esculentum), and phacelia (phacelia tanacetifolia) were sown in mixture with ryegrass (lolium multiflorum; intended biomass proportion of ...201121338812
near-full length sequencing of 16s rdna and rflp indicates that rhizobium etli is the dominant species nodulating egyptian winter berseem clover (trifolium alexandrinum l.).egyptian winter berseem clover (ewbc) is one of the main important forage legume crops in egypt that is used for animal feeding in winter and it occupies about 2.5 million feddans (feddan=4200m(2)) in winter agricultural rotation systems. forty-eight rhizobial isolates that nodulated this legume host from different geographical regions within egypt were isolated. rflp analyses of 16s rdna (1.5kb) and whole ribosomal dna (5kb), the sequencing of 16s rdna, and the sequencing of nodc, nifh and hous ...201424054695
bioavailability of selenium to forage crops in a sandy loam soil amended with se-rich plant materials.greenhouse experiments were conducted to study the bioavailability of selenium (se) to sorghum (sorghum bicolor l.), maize (zea mays l.) and berseem (trifolium alexandrinum l.) fodders in a sandy loam soil amended with different levels of se-rich wheat (triticum aestivum l.) and raya (brassica juncea l. czern) straw containing 53.3 and 136.7microg seg(-1), respectively. each of the fodder crops was grown after incorporation of se-rich materials either individually or in a sequence - sorghum-maiz ...200716919705
phytogrowth-inhibitory lactones derivatives of glaucolide b.the sesquiterpene lactone glaucolide b (1), isolated from vernonia fruticulosa (asteraceae), was transformed into six lactones (2-7). the structures of the products were elucidated by spectroscopic analysis. a series of solutions of compounds 1-7, at 200 microm, were tested on the germination and on the root and shoot growth of the dicotyledons physalis ixocarpa and trifolium alexandrinum and of the monocotyledons lolium multiflorum and amaranthus hypochondriacus. lactone 5 exhibited clear selec ...200915666538
evaluation of factors contributing to excessive nitrate accumulation in fodder crops leading to ill-health in dairy animals.a study was conducted to estimate nitrate content in commonly used fodder crops, viz., berseem (trifolium alexandrinum), bajra (pennisetum glaucum), maize (zea mays), oats (avena sativa), sorghum (sorghum vulgare) and toriya (brassica napus), collected from the fields of different villages of punjab and farms of the university, and to evaluate the factors associated with nitrate accumulation in these crops. the nitrate level was highest in sorghum on dry matter basis, followed by oats and toriya ...201121430916
effect of sewage water on mineral nutritive potential of six fodder species grown under semiarid conditions.investigation was performed to assess the effect of different sewage water treatments on the metal status of different fodder species consumed by ruminants under semi-arid conditions. five samples each of six fodder species viz., trifolium alexandrinum, cichorium intybus, avena sativa, medicago polymorpha, brassica campestris and medicago sativa were collected from three fields irrigated with canal water, mix water (canal water and sewage water) and sewage water, respectively. fodder samples wer ...201123961142
feed consumption, diet digestibility and mineral utilization in captive blackbuck (antelope cervicapra) fed different levels of concentrates.a feeding trial was conducted to know the level of concentrates in the diet of antelope cervicapra at which diet digestibility and mineral utilization were optimum. fifteen blackbucks (25-33 kg bw) were distributed into three groups of five each. fresh oat (avena sativa) and berseem (trifolium alexandrinum) fodders were offered ad libitum to all the animals. in addition, animals in groups ii and iii received concentrate mixture at the rate of 0.5 and 1% of bw, whereas animals in group i received ...201322093070
effect of concentrate supplementation on feed consumption, nutrient utilization and blood metabolite profile in captive spotted deer (axis axis) fed oat (avena sativa) and berseem (trifolium alexandrinum) fodders based diet.this experiment was conducted to determine the optimum level of a maize-soybean meal-wheat bran concentrate supplement fed to captive spotted deer fed an oat and berseem fodder-based diet. twelve adult spotted deer [64-76 kg body weight (bw)] were distributed into three groups of four each and were housed individually. a diet consisting of 5 kg of oat fodder and 5.5 kg of berseem fodder was offered to each one of the experimental animals. the animal in group i received no supplementary concentra ...201323349033
nutrient digestibility, ruminal fermentation activities, serum parameters and milk production and composition of lactating goats fed diets containing rice straw treated with pleurotus ostreatus.the study evaluated replacement of egyptian berseem clover (bc, trifolium alexandrinum) with spent rice straw (srs) of pleurotus ostreatus basidiomycete in diets of lactating baladi goats. nine lactating homo-parity baladi goats (average bw 23.8±0.4 kg) at 7 d postpartum were used in a triplicate 3×3 latin square design with 30 d experimental periods. goats were fed a basal diet containing 0 (control), 0.25 (srs25) and 0.45 (srs45) (w/w, dm basis) of srs. the control diet was berseem clover and ...201425049962
volatile seed germination inhibitors from plant residues.volatile emissions from residues of the winter cover legumes, berseem clover (trifolium alexandrinum l.), hairy vetch [vicia hirsuta (l.) s.f. gray], and crimson clover (trifolium incarnatum l.), inhibited germination and seedling development of onion, carrot, and tomato. using gc-ms, 31 c2-c10 hydrocarbons, alcohols, aldehydes, ketones, esters, furans, and monoterpenes were identified in these residue emission mixtures. mixtures of similar compounds were found in the volatiles released by herbi ...199024263582
germination and root elongation bioassays in six different plant species for testing ni contamination in soil.in vitro short-term chronic phytotoxicity germination and root elongation test were applied to test the effects of nickel (ni) in seed germination and root elongation in six plants species: cucumis sativus (cucurbitaceae), lepidium sativum and brassica nigra (brassicaceae), trifolium alexandrinum and medicago sativa (fabaceae), phacelia tanacetifolia (boraginaceae). a naturally ni rich soil was used to compare the results obtained. unlike root elongation, germination was not affected by ni in an ...201424288040
determination of nutrient contents and in vitro gas production values of some legume forages grown in the harran plain saline soils.the aim of this study was to determine the nutritive value of some legume species in salt-affected soils of south-east anatolian region using chemical composition and in vitro gas production kinetics. in this study, lotus corniculatus, trifolium alexandrinum, medicago sativa were sown and tested in four different locations. a 3 by 4 factorial design with 3 legume species and 4 salt levels (non salty electrical conductivity (ec)<4 ds/m; low salt: 4 ds/m>ec<8 ds/m, medium saline: 8 ds/m>ec<16 ds/m ...201425050020
antibacterial activity of leaves extracts of trifolium alexandrinum linn. against pathogenic bacteria causing tropical diseases.to investigate antibacterial potential of trifolium alexandrinum (t. alexandrinum) linn. against seven gram positive and eleven gram negative hospital isolated human pathogenic bacterial strains responsible for many tropical diseases.201223569896
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