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permanent genetic resources added to molecular ecology resources database 1 june 2010 - 31 july 2010.this article documents the addition of 205 microsatellite marker loci to the molecular ecology resources database. loci were developed for the following species: bagassa guianensis, bulweria bulwerii, camelus bactrianus, chaenogobius annularis, creontiades dilutus, diachasmimorpha tryoni, dioscorea alata, euhrychiopsis lecontei, gmelina arborea, haliotis discus hannai, hirtella physophora, melanaphis sacchari, munida isos, thaumastocoris peregrinus and tuberolachnus salignus. these loci were cro ...201021565125
reinventing the wheel and making it round again: evolutionary convergence in buchnera-serratia symbiotic consortia between the distantly related lachninae aphids tuberolachnus salignus and cinara cedri.virtually all aphids (aphididae) harbor buchnera aphidicola as an obligate endosymbiont to compensate nutritional deficiencies arising from their phloem diet. many species within the lachninae subfamily seem to be consistently associated also with serratia symbiotica we have previously shown that both cinara (cinara) cedri and cinara (cupressobium) tujafilina (lachninae: eulachnini tribe) have indeed established co-obligate associations with both buchnera and s. symbiotica however, while buchner ...201627190007
spontaneous electrical activity recorded from the aphid central nervous system.whilst many classes of insecticides target the insect central nervous system (cns), their effects in the cns of pest aphids have not been demonstrated. in this report, we describe an electrophysiological method for recording spontaneous neuronal activity from the giant willow aphid (tuberolachnus salignus). using extracellular recording electrodes and two analysis methods (threshold and template search), spontaneous spike activity was shown to exhibit sensitivity to the neuroexcitatory insectici ...201222996178
energy relations of solute loading in sieve elements of willow.experiments are described which attempt to clarify the quantitative relationship between sieve-tube loading of sucrose, and atp-turnover rates in the phloem of willow (salix viminalis l.). two experimental approaches have been made towards the solution of this problem. in the first of these the respiratory breakdown of (14)c-sugars was measured in segments of willow stem when no sieve-tube transport was taking place, and also under conditions where transport was occurring in response to the feed ...198724225872
measurement of the sieve tube membrane potential.a procedure is described for the measurement of the sieve tube membrane potential in the phloem of bark strips from salix exigua nutt. measurements were made by inserting a measuring microelectrode into sap exuding from severed stylets of the willow aphid, tuberolachnus salignus. data taken from 20 bark strips gave an average potential of -155 +/- 9 millivolts. evidence is presented for an electrogenic component of the sieve tube membrane potential. the occurrence of a saturable sucrose-induced ...198116661766
evidence for solution flow in the phloem of willow.sucrose specific mass transfer measurements were made in a translocating willow shoot (salix viminalis l.) by a steady state labelling technique and the translocate sucrose specific activity, concentration and velocity monitored by analysis of the honeydew from two colonies of the willow aphid tuberolachnus salignus gmelin. the values of sucrose smt obtained were related to the simultaneous measurements of translocate concentration and velocity and to the gradients of sucrose concentration withi ...197824413935
some evidence for the existence of turgor pressure gradients in the sieve tubes of willow.sieve tube sap was collected either from the severed stylets of tuberolachnus salignus (gmelin) or via incisions made into the phloem of small willow trees or potted cuttings. measurements of the osmotic potential (o.p.) of sap samples showed a gradient to exist in the presumed direction of assimilate transport, ie from apex to base of the stem.in most experiments samples of phloem tissue were taken after the collection of sieve tube sap, the water potential of these pieces of tissue being measu ...197524430219
the effect of low temperature on sucrose, atp and potassium concentrations and fluxes in the sieve tubes of willow.experiments have been performed on the effect of localised low (0°c) temperature application on solute concentration and fluxes in the sieve elements of willow. sieve tube exudate was obtained via the severed stylets of the aphid tuberolachnus salignus (gmelin). in stem segments, low temperature caused a fall in both the concentration and flux of sucrose. no recovery was observed during a 24 h cold application period. the concentrations of atp and potassium were generally also reduced, though th ...197224482275
patterns of translocation and metabolism of (14)c-labelled iaa in the phloem of willow.when 2-(14)-c-labelled iaa was applied to an isolated segment of willow via a bark abrasion the pathway of transport of this compound was found to be located in the sieve elements as evidenced by the pattern of activity found in honeydew excreted by individuals of tuberolachnus salignus (gmelin) feeding on the segment.further experiments have established that polarity of transport of (14)c-iaa occurs in a basipetal direction when isolated segments of willow are orientated in a vertical position, ...197124493043
studies on the movement of indole auxins in willow (salix viminalis l.).auxin activity was detected in honeydew obtained from the aphid tuberolachnus salignus (gmelin) feeding on willow (salix viminalis). active uptake of (14)c-indolyl-3-acetic acid (iaa) into the sieve tubes was demonstrated by irrigating the cambial surface of willow bark with (14)c-iaa solution and assaying aphid stylet exudate. when, however, (14)c-iaa was applied to the peridermal tissues of the bark or to a mature leaf most of the radioactivity (collected in honeydew or stylet exudate) co-chro ...197124487450
some effects of iaa and kinetin upon the movement of sugars in the phloem of willow.isolated bark strips of willow were sealed on to polythene tubes having three compartments. colonies of the aphid tuberolachnus salignus gmelin were established on the bark at each end of the strip. iaa or kinetin at a concentration of 10(-5)m was applied to the cambial surface of the strip in one of the end compartments, whilst either (14)c-labeled sucrose or (86)rbcl was applied in the centre compartment.both iaa and kinetin caused the activity from the (14)c-sucrose to move away from the area ...197024499835
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