| bacterial diversity in a nonsaline alkaline environment: heterotrophic aerobic populations. | heterotrophic populations were isolated and characterized from an alkaline groundwater environment generated by active serpentinization, which results in a ca(oh)2-enriched, extremely diluted groundwater with ph 11.4. one hundred eighty-five strains were isolated in different media at different ph values during two sampling periods. to assess the degree of diversity present in the environment and to select representative strains for further characterization of the populations, we screened the is ... | 2004 | 15574939 |
| microcella putealis gen. nov., sp. nov., a gram-positive alkaliphilic bacterium isolated from a nonsaline alkaline groundwater. | three gram-positive bacteria designated cv-2t, cv-40 and ac-30 were isolated from a highly alkaline groundwater environment (ph 11.4). these organisms formed very small rod-shaped cells, are aerobic, non-spore-forming, catalase-positive, oxidase-negative, with an optimum growth temperature of 35 degrees c and optimum ph value of growth between 8.5 and 9.0. the strains possessed a novel b-type cell-wall peptidoglycan structure with lysine as the diamino acid; the major respiratory quinones were m ... | 2005 | 16104348 |
| frigoribacterium faeni gen. nov., sp. nov., a novel psychrophilic genus of the family microbacteriaceae. | the taxonomic position of five actinobacterial strains isolated from dust, an animal shed, the air inside a museum and soil was investigated using a polyphasic approach. the growth characteristics were unusual for actinomycetes. optimal growth was at temperatures ranging from 2 to 10 degrees c. after small-step adaptation (5 degrees c steps) to higher temperatures, the strains were also able to grow at 20 degrees c. cell wall analyses revealed that the organisms showed a hitherto undescribed, ne ... | 2000 | 10826823 |
| 16s rrna gene-based identification of cultured bacterial flora from host-seeking ixodes ricinus, dermacentor reticulatus and haemaphysalis concinna ticks, vectors of vertebrate pathogens. | a total of 151 bacterial isolates were recovered from different developmental stages (larvae, nymphs and adults) of field-collected ticks (67 strains from ixodes ricinus, 38 from dermacentor reticulatus, 46 from haemaphysalis concinna). microorganisms were identified by means of 16s rrna gene sequencing. almost 87 % of the strains belonged to g(+) bacteria with predominantly occurring genera bacillus and paenibacillus. other g(+) strains included arthrobacter, corynebacterium, frigoribacterium, ... | 2009 | 19937215 |
| molecular identification of airborne bacteria associated with aerial spraying of bovine slurry waste employing 16s rrna gene pcr and gene sequencing techniques. | polymerase chain reaction amplification of the universal 16s ribosomal rna (rrna) gene was performed on a collection of 38 bacterial isolates, originating from air sampled immediately adjacent to the agricultural spreading of bovine slurry. a total of 16 bacterial genera were identified including both gram-positive and gram-negative genera. gram-positive organisms accounted for 34/38 (89.5%) of total bacterial numbers consisting of 12 genera and included staphylococcus (most common genus isolate ... | 2010 | 20018377 |
| frigoribacterium mesophilum sp. nov., a mesophilic actinobacterium isolated from bigeum island, korea. | a taxonomic study was performed on strain msl-08t, which was isolated from a soil sample collected from bigeum island. the novel isolate was aerobic and gram-positive. cells were short and motile rods. growth temperature ranged from 20 to 28 degrees c and the ph for growth ranged from 6.5 to 12.0. the optimum growth temperature and ph were 28 degrees c and 7.3, respectively. the predominant menaquinone was mk-9. cell wall analysis showed b-type peptidoglycan containing 2,4-diaminobutyric acid, a ... | 2008 | 18676471 |
| compostimonas suwonensis gen. nov., sp.nov., isolated from spent mushroom compost. | strain smc46t, a gram-positive, aerobic, non-motile, short rod-shaped bacterium, was isolated from spent mushroom compost sample collected in the suwon region, south korea. 16s rrna gene sequence analysis revealed that strain smc46t was a member of the family microbacteriaceae, however, formed an independent branch separate from other genera within the family microbacteriaceae. sequence similarity levels between strain smc46t and other members of the family microbacteriaceae were below 97%, show ... | 2011 | 22140170 |
| isolation of bacterial endophytes from germinated maize kernels. | the germination of surface-sterilized maize kernels under aseptic conditions proved to be a suitable method for isolation of kernel-associated bacterial endophytes. bacterial strains identified by partial 16s rrna gene sequencing as pantoea sp., microbacterium sp., frigoribacterium sp., bacillus sp., paenibacillus sp., and sphingomonas sp. were isolated from kernels of 4 different maize cultivars. genus pantoea was associated with a specific maize cultivar. the kernels of this cultivar were ofte ... | 2007 | 17668041 |
| subtercola boreus gen. nov., sp. nov. and subtercola frigoramans sp. nov., two new psychrophilic actinobacteria isolated from boreal groundwater. | psychrophilic actinobacterial isolates from permanently cold groundwater in finland were characterized using a polyphasic approach. growth on agar plates was observed at temperatures down to -2 degrees c, with an optimum at 15-17 degrees c, but no growth was observed at 30 degrees c. the peptidoglycan type was b2y and the characteristic diamino acid was diaminobutyric acid. the cell wall sugars of strain k265t were rhamnose, ribose, xylose and mannose and those of strain k300t were glucose, rham ... | 2000 | 11034481 |
| labedella gwakjiensis gen. nov., sp. nov., a novel actinomycete of the family microbacteriaceae. | a gram-positive, non-motile, non-mycelium-forming, rod-shaped actinomycete, designated ksw2-17t, was isolated from dried seaweed collected from beach sand along the coast of jeju, republic of korea. the organism had ornithine as the diagnostic cell-wall diamino acid, mk-10 and mk-11 as the major menaquinones, and phosphatidylglycerol and diphosphatidylglycerol as polar lipids. the fatty acid profile included predominantly iso- and anteiso-branched acids and a minor amount of tuberculostearic aci ... | 2007 | 17978208 |