| isolation and disruption of the melanin pathway polyketide synthase gene of the softwood deep stain fungus ceratocystis resinifera. | ceratocystis resinifera hyphae produce a black melanin pigment causing a deep stain in softwood logs. we exploited the homology of polyketide synthases to clone pks1, a gene responsible for dihydroxynaphthalene-melanin biosynthesis in c. resinifera. sequence analysis indicated that pks1 has two introns near its 5(') end and encodes a 2188-amino acid polypeptide with five functional domains: beta-ketoacyl synthase, acyl transferase, two acyl carrier proteins and a thioesterase/claisen cyclase. a ... | 2004 | 14643257 |
| bioprotection of spruce logs against sapstain using an albino strain of ceratocystis resinifera. | abstract we recovered a spontaneous albino strain from ascospores of ceratocystis resinifera, a sapstain fungus that grows deeply and rapidly in freshly felled conifer trees. this albino strain, named kasper, was tested for its ability to prevent discoloration of spruce sapwood caused by wild-type sapstain fungi and compared with cartapip 97, a commercially available biological control agent of sapstain in lodgepole pine and red pine logs. in a laboratory trial, kasper reduced sapstain of white ... | 2006 | 18944313 |
| genetic variability and structure of canadian populations of the sapstain fungus ceratocystis resinifera. | abstract genomic dna was extracted from 129 isolates of ceratocystis resinifera, a species belonging to the c. coerulescens complex, and 19 polymorphic random amplified polymorphic dna markers were used to study the population genetic structure of this fungus. the analysis suggested a moderate value for genetic diversity (h(s) = 0.209). however, when monomorphic markers and rare alleles, representing 89 markers, also were included in the calculation, the genetic diversity of canadian populations ... | 2004 | 18943702 |
| a spontaneous albino mutant of ceratocystis resinifera results from a point mutation in the polyketide synthase gene, pks1. | we characterized a spontaneous albino mutant of ceratocystis resinifera. compared with the wild-type progenitor strain, the albino mutant had a reduced linear growth on culture medium, but its growth on lodgepole pine sapwood was unaffected. the albino mutant did not produce any coloured pigment on agar media or wood. however, upon exposure to exogenous scytalone, an intermediate metabolite of the melanin pathway, the production of a brownish melanin was restored. this suggests that the albino p ... | 2006 | 16788717 |