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[two cases of paragonimiasis westermani in a chinese family diagnosed with the ouchterlony double diffusion method].we report two cases of paragonimus westermani infection in a chinese family in japan. a 41-year-old husband and his 40-year-old wife were infected with p. westermani after consuming a homemade chinese traditional "drunken crab." they were a family with two children who had lived in japan for 19 years. the crabs were eriocheir japonica sent from the kyusyu area that they had pickled at home with soy sauce and chinese liquor for 5 days. their children did not eat any of the crabs. one month after ...201425764810
infection status of freshwater crabs and crayfish with metacercariae of paragonimus westermani in korea.the present study investigated the infection status of paragonimus westermani metacercariae in freshwater crabs (n = 363) and crayfish (n = 31) from october 2007 to october 2008 using the crush method. all of the freshwater crabs, eriocheir japonicus, were negative for p. westermani metacercariae while 10 (32.3%) of the 31 examined crayfish were positive. the 10 positive crayfish were caught in haenam, jeollanam-do, and there were 8-59 (mean 28.4) metacercariae per infected crayfish. these resul ...200919967096
human pulmonary distomiasis caused by paragonimus westermanni.1. the morbidity of pulmonary distomiasis among the school children in the plains of the prefecture of shinchiku is 4.3 per cent, while in the mountainous regions among the savages it reaches in some districts 50 per cent. 2. seventeen species of cercariae were discovered in fresh water mollusks in the prefecture of shinchiku, formosa. but it was impossible to ascertain from morphological characteristics alone which of them developed into the pulmonary fluke. consequently, the eggs of the pulmon ...191719868151
mitochondrial dna-inferred population structure and demographic history of the mitten crab (eriocheir sensu stricto) found along the coast of mainland china.the mitten crab, eriocheir sensu stricto, is economically important in east asia, although it is an invasive species in europe and north america. little is known about its population structure and historical demography in its native range, especially along the pacific coast of china. we collected mitochondrial cytochrome oxidase subunit ii and cytochrome b sequences from 154 individuals distributed in the rivers along the chinese coast and 15 individuals from japan. phylogenetic analysis resulte ...200819160479
studies on the metabolism of the hosts of paragonimus westermani (kerbert, 1878). ii. paper chromatography of some metabolites in the blood of eriocheir japonicus (de haan). 196414290452
the effects of tetrodotoxin on the slow potential and spikes in the cardiac ganglion of a crab, eriocheir japonicus. 19715317261
[two cases of paragonimus westermani in one family].a 68-year-old woman and her 28-year-old son ate some rare fresh-water crabs (eriocheir japonicus). the son became ill one month later with pleural effusion. after 10 months the mother's symptoms included cough, hemosputum and a nodular shadow on her chest x-ray. eosinophilia was present and the ouchterlony test showed strong bands toward paragonimus westermani antigen in both patients. they were diagnosed as having paragonimiasis westermani. the symptoms stopped after bithionol administration an ...19948084104
epidemiologic studies of paragonimus westermani (kerbert, 1878) and related species in taiwan (formosa). ii. seasonal variations in the incidence of paragonimus westermani infection in eriocheir japonicus. 19665231982
metacercarial infections of paragonimus westermani in freshwater crabs sold in markets in seoul.status of metacercarial infections of paragonimus westermani was observed in freshwater crabs, which were purchased at 3 markets in its peak season of 1990. all of 85 crabs were eriocheir japonicus. no other species of eriocheir were found. when crushed muscle and viscera was examined individually, the infection rate was 11.8%; and mean number of metacercariae was 2.1 per infected crab. unless adequately cooked, freshwater crabs are still potential sources of human paragonimiasis.19911954202
[gonadogenesis and color characteristics of ovaries in japanese mitten crab eriocheir japonicus].the gonadogenesis was studied in adult and juvenile females of japanese mitten crab eriocheir japonicus (crustacea: decapoda, grapsida) inhabiting the rivers of the maritime territory. the morphometric parameters of oocytes at different stages of maturity were determined using the methods of computer morphometry and color characteristics were evaluated using the munsell book of color. as a result, a color table was compiled for the ovaries from the beginning of development to gonad maturity, whi ...200818409381
molecular cloning, mrna expression, and characterization of hsp90 gene from chinese mitten crab eriocheir japonica sinensis.hsp90 is a highly conserved molecular chaperone important in the maturation of a broad spectrum of proteins. using expressed sequence tag (est) and rapid amplification of cdna ends (race) techniques, an hsp90 gene designated as ejshsp90 was cloned and characterized from the chinese mitten crab eriocheir japonica sinensis. the full-length cdna of ejshsp90 is 2,517 bp and contains an open reading frame of 2157 bp which encodes a 718 amino acid polypeptide (82.8 kda) bearing characteristics of the ...200919166961
identification, mrna expression and characterization of a novel ank-like gene from chinese mitten crab eriocheir japonica sinensis.ankyrins are a family of adapter molecules mediating linkages between integral membrane and cytoskeletal proteins. ankyrin repeat is one of the most frequently observed amino acid motifs in protein databases. a novel ank-like gene of chinese mitten crab eriocheir japonica sinensis (denoted as ejsank) was identified and cloned by expressed sequence tag and rapid amplification of cdna end approaches. the full-length cdna of ejsank is 4375 bp and contains an open reading frame of 1095 bp which enco ...200919389480
studies on the interaction between benzidine and hemocyanin from chinese mitten crab eriocheir japonica sinensis (decapoda, grapsidae).the interaction of benzidine (bz) with hemocyanin (hc) from chinese mitten crab (eriocheir japonica sinensis) was studied by fluorescence spectrum. bz can quench the fluorescence of hc by forming a new complex. the process of binding bz on hc was a spontaneous molecular interaction procedure. the thermodynamic parameters, the enthalpy change and entropy change were estimated to be 47.36 kj mol(-1), 248.51 j mol(-1) k(-1) according to the van' hoff equation. this indicates that hydrophobic intera ...200919419903
identification and characteristics of a novel gene, ejo1, in the chinese mitten crab (eriocheir japonica sinensis) ovary.ejo1, a novel gene presumably involved in the ovary development of the chinese mitten crab (eriocheir japonica sinensis), was identified and characterized by suppression subtractive hybridization and cdna macroarray analysis. ejo1 expression was 2.6-fold higher at stage iii than at stage ii during the ovary development of the mitten crab. ejo1 is 876 bp in length containing a 759 bp open reading frame which encodes a 252-amino-acid protein. homology analysis showed that no sequence significantly ...201019728148
identification, mrna expression and characterization of proliferating cell nuclear antigen gene from chinese mitten crab eriocheir japonica sinensis.the sliding clamp proliferating cell nuclear antigen (pcna) plays important roles in nucleic acid metabolism. in this work, we isolated a pcna gene (designated as ejspcna, accession: fj483830) by rapid amplification of cdna ends approach from the chinese mitten crab eriocheir japonica sinensis. the full-length cdna of ejspcna consists of 1123 nucleotides with an open-reading frame of 780bp encoding 259 amino acids (28.62kda) and containing an interdomain connecting loop, c-terminal tail, and cen ...201020542131
identification of differentially expressed genes during the brachyurization of the chinese mitten crab eriocheir japonica sinensis.expressed sequence tags (ests) obtained from two-directional suppression subtractive hybridization (ssh) of cdna libraries derived from the pleon of the chinese mitten crab were analyzed using mrna subtractive hybridization. after cdna cloning and sequencing, gene expression profiles were examined, focusing on brachyurization. we assembled 211 non-redundant ests from ssh library 1 (yielding 130 unique transcripts: 25 consensi, 105 singletons) and 669 from ssh library 2 (195: 51 consensi, 144 sin ...201121607665
[no- and h2s-synthesizing neurons in thoracic ganglion of crabs eriocheir japonicus and pugettia quadreidens (decapoda)].by methods of histochemical marking of nadph-diaphorase and immunohistochemical marking of cystathionine-beta-synthase (cbs), the presence and distribution of no and h2s-synthesizing elements in thoracic ganglion are shown in two crab species eriocheir japonicus and pugettia quadreidens. in the thoracic ganglion, no and h2s have been established to be synthesized by different neurons. the high cbs activity in efferent elements--segmentary motoneurons, interneurons, and nerve fibers in the extrem ...201322645979
comparative phylogeography of two marine species of crustacean: recent divergence and expansion due to environmental changes.environmental changes, such as changes in the coastal topography due to eurasian plate movements, climate oscillation during the pleistocene, and alteration of ocean currents, have complicated the geographical structure of marine species and deepened their divergence between populations. as two widely distributed species of crustacean (oratosquilla oratoria and eriocheir japonica), weak differences were expected due to their high dispersal potential of planktonic larvae with ocean currents. howe ...201425106858
[fertility and embryonic mortality level of the japanese mitten crab eriocheir japonica (crustacea: decapoda: varunidae) in primorye].reproductive biology of the japanese mitten crab eriocheir japonica (de haan, 1835) was studied in the northern part of its habitat. it was revealed that e. japonica from the water bodies of primorye, in contrast to crabs in the central part of the habitat on the japanese islands, lays fewer eggs in the first clutch, does not have direct dependence between the size-weight characteristics of females and the number of eggs in the third clutch, and shows higher level of embryonic mortality. the los ...201626859967
[localization and quantitative assessment of oxygen-dependent hypoxia-inducible factor 1α in the brain of mitten crab eriocheir japonica in norm and in acute anoxia (an immunohistochemical study)].using immunoblotting and immunocytochemistry, the expression of hypoxia-inducible factor 1α (hif-1α) was studied in the brain of the mitten crab eriocheir japonica in norm and at 2, 4, 6 and 12 hours of anoxia on the model of water deprivation. in intact crabs, the number of immunopositive neurons was small, but it increased with anoxia duration. particularly pronounced increase in the proportion of neurons with the hif-1α expression was found in cell group 6. in group 9/11. the highest expressi ...201627487659
mitochondrial genome of the chinese mitten crab eriocheir japonica sinenesis (brachyura: thoracotremata: grapsoidea) reveals a novel gene order and two target regions of gene rearrangements.we determined the complete 16,354 bp mitochondrial dna sequence of the chinese mitten crab eriocheir japonica sinesnesis. it consists of 13 protein coding genes (pcgs), 2 rrnas, and 22 trnas, typical of metazoan mitochondrial genomes. with respect to the ancestral crustacean mt gene order, a pcg, the rrnas, and 12 of the trnas appear to be rearranged. this rearrangement is novel in the arthropods and suggests an accelerated rate of mt genome rearrangement in this brachyuran lineage based on the ...200515780981
inactivation of paragonimus westermani metacercariae in soy sauce-marinated and frozen freshwater crabs.soy sauce-marinated freshwater crabs (eriocheir japonicus) are a source of human paragonimiasis. the viability of paragonimus westermani metacercariae (pwmc) in marinated crabs was investigated in an experimental setting. the pwmc collected from freshwater crayfish were inoculated into freshwater crabs, which were then frozen or marinated in soy sauce. all pwmc in the freshwater crabs were inactivated after freezing for 48 h at -20 °c and after freezing for 12 h at -40 °c. after marinating for 3 ...201728127717
p450 in wild animals as a biomarker of environmental impact.the impact of environmental pollution on selected animals was tested by monitoring the hepatic content of cytochromes p450 and their enzyme activities or by calculating teq values from the concentration of pollutants in the body. fish-eating stellars sea eagles, haliaeetus pelagicus, found dead in the northern part of hokkaido island accumulated high levels of pcbs and ddt and metabolites. the teq values calculated from the pcb concentration in the eagles were high enough to cause a significant ...200123886053
[paragonimiasis westermani associated with pseudochylothorax].a 67-year-old woman was referred to our hospital because of right pleural effusion. she had eaten boiled crab, eriocheir japonicus, raw crab of an unknown species, and broiled wild boar several years ago. the punctured pleural fluid was characterized by an exudative nature, milky-white color, and high cholesterol concentration, indicating pseudochylothorax. high titers of anti-paragonimus westermani antibody in the pleural fluid and serum, assessed by microplate elisa, established the diagnosis ...200919882911
alterations of cytochrome p450-dependent monooxygenase activities in eriocheir japonicus in response to water pollution.eriocheir japonicus, fresh-water crabs inhabiting rivers and estuaries in japan, were investigated for cytochrome p450 (cyp)-dependent drug-metabolizing enzyme activities to see if these activities reflect the river pollution gradient. from the laboratory dose-response experiments, we found that the polycyclic aromatic hydrocarbon (pah) 3-methylcholanthrene induced total cyp contents, ethoxycoumarin o-deethylase activity, and bunitrolol 4-hydroxylase activity in crab hepatopancreas. in the field ...19968841764
the burst activity of different cell regions and intercellular co-ordination in the cardiac ganglion of the crab, eriocheir japonicus. 19724651665
spermatogenesis in animals as revealed by electron microscopy. vii. spermatid differentiation in the crab, eriocheir japonicus.testes of eriocheir japonicus were fixed in buffered (ph 7.2) 1 per cent osmium tetroxide and thin sections of the methacrylate-embedded tissue were studied with the electron microscope. spermatozoa from the vasa deferentia and spermatids from the testes were examined in smear preparations and sections. the most useful stainings proved to be the feulgen, unna-pappenheim, and pas reactions. the present paper covers one of the late stages of spermatogenesis. at the late stage of differentiation of ...196013846483
biology of paragonimus westermani (kerbert, 1878) braun, 1899: infection in the crab host (eriocheir japonicus de haan) on taiwan. 19695764078
paragonimus and paragonimiasis in korea.the lung fluke, paragonimus, and lung fluke disease, were reviewed, especially on the works performed in korea. among 43 species of paragonimus recorded in the world literature, p. westermani, p. pulmonalis, and p. iloktsuenensis are known to distribute in korea. biological studies on p. westermani have revealed that its snail intermediate host is semisulcospira spp. and major second intermediate hosts are crabs such as eriocheir japonicus and e. sinensis or crayfish such as cambaroides similis ...19902133425
paragonimus westermani: life cycle, intermediate hosts, transmission to man and geographical distribution in korea.since discovery of this lung fluke in 1878 by kerbert, paragonimus westermani has been found to occur in many parts of asia. recently, however, it was detected that there exists a bisexual type and also a parthenogenetic type of the lung fluke which has been called p. westermani for a century. the natural definitive hosts other than man include a wide range of mammals. adult worms are localized in the lungs. the eggs excreted with sputum and reaching water develop into miracidia in water. miraci ...19846542389
molecular systematics of the asian mitten crabs, genus eriocheir (crustacea: brachyura).to help resolve phylogenetic relationships among the mitten crabs, complete sequences of the nuclear dna internal transcribed spacer (its) and portions of the mitochondrial genome corresponding to the cytochrome oxidase i (coi), were sequenced for all asian mitten crabs of the genus eriocheir and seven species of the grapsoidea. the resulting phylogeny supports the establishment of a separate genus neoeriocheir, but does not provide justification for the recognition of platyeriocheir. a female m ...200313678686
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