| [effect of different salts on beta-hydroxyacyl-coa dehydrogenase and beta-acetoacetyl-coa thiolase activities in muscles of eriocheir sinensis and homarus vulgaris]. | | 1972 | 4114869 |
| variation in lipid composition of chinese mitten-handed crab, eriocheir sinensis during ovarian maturation. | this experiment was conducted to investigate the variation in lipid composition during the ovarian maturation of the crab eriocheir sinensis. the chinese mitten-handed crab broodstock was divided into six different maturation periods according to the size and color of ovary. ovary, hepatopancreas, muscle, and hemolymph of broodstock in different maturation periods were analyzed for total lipid and fatty acids using gas chromatography, and lipid classes by thin-layer chromatography. the ovarian l ... | 2001 | 11470448 |
| the environmental consequences of alien species in the swedish lakes mälaren, hjälmaren, vänern and vättern. | twenty alien species have become established in the lakes mälaren, vänern, vättern and hjälmaren. intentional introductions include fish and the signal crayfish from north america, ornamental plants, and the canada goose. unintentional introductions include the crayfish plague introduced with infected crayfish, the zebra mussel, and chinese mitten crab introduced with ballast water. the introduction of pathogens and parasites, in particular the crayfish plague, to the lakes has had the greatest ... | 2001 | 11878025 |
| rickettsia-like organism associated with tremor disease and mortality of the chinese mitten crab eriocheir sinensis. | we report the discovery of a rickettsia-like organism (rlo) in cultured freshwater chinese mitten crab eriocheir sinensis. the rlo caused tremor disease and was apparently responsible for a mass mortality (30 to 90%) in 2 provinces in southeast china. moribund crabs were investigated from different districts during outbreaks in 1999 and 2000. with electron microscopy, 3 different pathogens were detected in moribund crabs: a rickettsia-like organism (rlo), virus-like particles (vlp) and a microsp ... | 2002 | 12005237 |
| molecular systematics of the asian mitten crabs, genus eriocheir (crustacea: brachyura). | to help resolve phylogenetic relationships among the mitten crabs, complete sequences of the nuclear dna internal transcribed spacer (its) and portions of the mitochondrial genome corresponding to the cytochrome oxidase i (coi), were sequenced for all asian mitten crabs of the genus eriocheir and seven species of the grapsoidea. the resulting phylogeny supports the establishment of a separate genus neoeriocheir, but does not provide justification for the recognition of platyeriocheir. a female m ... | 2003 | 13678686 |
| study on experimental infections of spiroplasma from the chinese mitten crab in crayfish, mice and embryonated chickens. | tremor disease (td) of the chinese mitten crab eriocheir sinensi has become a serious disease in the chinese freshwater culture industry in recent years. the agent, belonging to spiroplasma, was purified from yolk and allantoic fluids of chicken embryos and was then inoculated into the body of crayfish, the abdominal cavity of icr mice and the allantoid of chicken embryos, respectively. thirty-eight days after the inoculation, the mice and crayfish were dissected and their tissues sampled and ob ... | 2003 | 14643405 |
| the first internal transcribed spacer (its-1) of ribosomal dna as a molecular marker for phylogenetic and population analyses in crustacea. | the objective of the present study is to explore the feasibility of using the first internal transcribed spacer (its-1) of ribosomal dna as a molecular marker for studying the interspecific and intraspecific genetic variations among crustaceans. we designed primers that could amplify its-1 from a majority of taxonomic groups of crustaceans. the gene was found to exhibit a high degree of length polymorphism among different groups, ranging from 182 bp in the barnacle balanus amphitrite to approxim ... | 2001 | 14961351 |
| purification of vitellin from the ovary of chinese mitten-handed crab (eriocheir sinensis) and development of an antivitellin elisa. | vitellin was purified from ovaries of mature female chinese mitten-handed crab (eriocheir sinensis) using gel filtration chromatography. analysis by native page showed the vitellin had a native molecular mass of 520 kda, while denaturing sds-page revealed two subunits of 97 and 74 kda. purified vitellin was used to raise polyclonal antisera, with which an enzyme-linked immunosorbent assay (elisa) was developed. the elisa was sensitive and could effectively detect vitellin in the range of 7.8-500 ... | 2004 | 15253879 |
| a spiroplasma associated with tremor disease in the chinese mitten crab (eriocheir sinensis). | an epidemic of tremor disease has been a serious problem in chinese mitten crabs, eriocheir sinensis, in china in recent years. the disease-causing agent was previously considered to be a rickettsia-like organism. here, analysis of the 16s rrna gene sequence, light and electron microscopy and cultivation in vitro were used to identify the agent. sequence analysis of the 16s rrna gene found it to have 98 % identity with that of spiroplasma mirum. the agent was able to be passed through membrane f ... | 2004 | 15347761 |
| morphology of spiroplasmas in the chinese mitten crab eriocheir sinensis associated with tremor disease. | the structure of spiroplasmas in the chinese mitten crab associated with tremor disease was studied by transmission electron microscopy techniques, including both negative staining and ultrasectioning. a spiral structure, which is a typical form of spiroplasmas, could be detected and the observations showed for the first time the presence of spiroplasmas in the cells of the crab. propagation of spiroplasmas within the cells presented various forms that could be sorted into three morphological ty ... | 2004 | 15380550 |
| purification and characterization of a new reovirus from the chinese mitten crab, eriocheir sinensis. | a new reovirus was recently isolated from a freshwater crab, the chinese mitten crab, eriocheir sinensis, in china. the complete viral particles are 55 nm in diameter, icosahedral, non-enveloped and have a mean buoyant density of 1.39 g cm(-3) in cscl gradient. the viral genome is composed of 12 pieces of dsrna with an electrophoretic pattern of 3/4/2/3. this virus infects connective tissue of the gills, gut and hepatopancreas. partial cdna cloning and sequence analysis showed that the rna-depen ... | 2004 | 15575876 |
| a novel spiroplasma pathogen causing systemic infection in the crayfish procambarus clarkii (crustacea: decapod), in china. | a novel disease of crayfish procambarus clarkii appeared in the summer of 2004 in freshwater aquaculture in jiangsu province of china. light and transmission electron microscopy (tem), molecular biological methods and in vitro culture were used to identify the pathogen. the agent was unique in having a helical morphology and rotary motility as observed by phase-contrast light microscopy and was found in haemolymph, muscles, nerves and connective tissues by smear method and tem. ultra-thin sectio ... | 2005 | 16000238 |
| ultrastructural study on a novel microsporidian, endoreticulatus eriocheir sp. nov. (microsporidia, encephalitozoonidae), parasite of chinese mitten crab, eriocheir sinensis (crustacea, decapoda). | a microsporidian pathogen, infecting the epithelial cells of the hepatopancreas of chinese mitten crab, eriocheir sinensis, was studied by electron microscopy. the detailed ultrastructure of life cycle of the pathogen including proliferative and sporogonic developmental stages are provided. all stages of the parasite are haplokaryotic and develop in a vacuole bounded by a single membrane in contact with host cell cytoplasm. sporogenesis is synchronous with the same developmental stage in one vac ... | 2007 | 17097104 |
| occurrence and density of vibrio parahaemolyticus in live edible crustaceans from markets in china. | vibrio parahaemolyticus is a marine bacterium causing foodborne disease. occurrence of the bacterium was investigated in six species of edible crustaceans available from markets in mainland china. the bacterium was detected in 22 of 45 whole-body, shell, and feces samples, including mitten crabs, which are supposed to be produced in freshwater ponds. the mean densities ranged from 2.8 log cfu/g in mitten crabs (eriocheir sinensis) to 5.1 cfu/g in giant tiger prawns (penaeus monodon). in hemolymp ... | 2006 | 17133820 |
| effect of waterborne copper on the microstructures of gill and hepatopancreas in eriocheir sinensis and its induction of metallothionein synthesis. | effects of different waterborne copper concentrations (0.00, 0.01, 0.10, 1.00, and 5.00 mg/l) on microstructures of gill and hepatopancreas, and in inducing metallothionein (mt) synthesis were studied in chinese mitten crab eriocheir sinennsis. the results indicated that copper accumulation caused histologic damage to gill and hepatopancreas and induced mt synthesis in hepatopancreas. no significant differences were found in microstructures between 0.01 mg/l copper treatment and the control grou ... | 2007 | 17165106 |
| genetic population structure and contemporary dispersal patterns of a recent european invader, the chinese mitten crab, eriocheir sinensis. | genetic studies of recently established populations are challenging because the assumption of equilibrium underlying many analyses is likely to be violated. using microsatellites, we investigated determinants of genetic structure and migration among invasive european-chinese mitten crab populations, applying a combination of traditional population genetic analyses and nonequilibrium bayesian methods. consistent with their recent history, invasive populations showed much lower levels of genetic d ... | 2007 | 17217341 |
| acute toxicity of the pesticides chlorpyrifos and atrazine to the chinese mitten-handed crab, eriocheir sinensis. | | 2006 | 17219314 |
| [expression and purification of recombinant metallothionein of eriocheir sinensis in e. coli]. | metallothioneins (mt) are potential candidates for medicine development and application. for the purpose of expressing recombinant mt in e. coli, a crab mt cdna cloned into pgem-t was subcloned into pet-gst and then transformed into escherichia coli bl21. the fusion protein was proved to be expressed in both soluble and insoluble form by sds-page and western blot. since metallothionein chelate metal ions, which may effects the physiological process of e. coli, caused the production of recombinan ... | 2007 | 17591271 |
| phylogenetic analysis of intestinal bacteria in the chinese mitten crab (eriocheir sinensis). | to identify the dominant intestinal bacteria in the chinese mitten crab, and to investigate the differences in the intestinal bacteria between pond-raised and wild crabs. | 2007 | 17714401 |
| a prophenoloxidase from the chinese mitten crab eriocheir sinensis: gene cloning, expression and activity analysis. | prophenoloxidase (propo) is a conserved copper-containing enzyme that plays important roles in immune response of crustaceans and insects. in the present study, the full-length cdna of a prophenoloxidase (designated espropo) was cloned from haemocytes of chinese mitten crab eriocheir sinensis by expressed sequence tag (est) and pcr techniques. the isolated 3549bp full-length cdna of espropo contained a 2040bp open reading frame (orf) encoding a putative propo protein of 679 amino acids, a 5'-unt ... | 2008 | 18160310 |
| molecular cloning, genomic organization and functional analysis of an anti-lipopolysaccharide factor from chinese mitten crab eriocheir sinensis. | anti-lipopolysaccharide factor (alf) represents one kind of basic proteins, which binds and neutralizes lps and exhibits strong antibacterial activity against gram-negative r-type bacteria. the alf gene of chinese mitten crab eriocheir sinensis (milne edwards, 1853) (denoted as esalf) was identified from haemocytes by expressed sequence tag (est) and pcr approaches. the full-length cdna of esalf consisted of 700 nucleotides with a canonical polyadenylation signal-sequence aataaa, a polya tail, a ... | 2008 | 18206230 |
| improvement of innate immune responses and defense activity in mitten crab (eriocheir sinensis) by oral administration of beta-glucan. | the beta-glucan (bg), extracted from saccharomyces cerevisiae cell wall, was orally administrated to the mitten crab (eriocheir sinensis) at 0, 1, 5 and 10 g bg/kg diet for 28 days, followed by a challenge with vibrio mimicus by intramuscular injection. growth, phenoloxidase, superoxide dismutase, acid phosphatase and alkaline phosphatase activity were monitored after 14 and 28 days. the results showed an immunomodulatory effect and protection against v. mimicus by dietary supplementation of bg. ... | 2008 | 18568402 |
| phylogenetic analysis of spiroplasmas from three freshwater crustaceans (eriocheir sinensis, procambarus clarkia and penaeus vannamei) in china. | disease epizootics in freshwater culture crustaceans (crab, crayfish and shrimp) gained high attention recently in china, due to intensive developments of freshwater aquacultures. spiroplasma was identified as a lethal pathogen of the above three freshwater crustaceans in previous studies. further characterization of these freshwater crustacean spiroplasma strains were analyzed in the current study. phylogenetic position was investigated by analysis of partial nucleotide sequences of 16s ribosom ... | 2008 | 18621053 |
| cdna cloning, characterization and mrna expression of a pacifastin light chain gene from the chinese mitten crab eriocheir sinensis. | the pacifastin family, characterized by several conserved arrays of six cysteine residues, is a newly identified serine protease inhibitor (spi) family discovered uniquely in arthropods and plays important roles in multiple biological processes. in the present study, the full-length cdna of a pacifastin light chain (designated esplc) was cloned from the chinese mitten crab eriocheir sinensis by expressed sequence tags (ests) and pcr techniques. the 1036bp esplc cdna contained an 831bp open readi ... | 2008 | 18817876 |
| molecular cloning and characterization of peroxiredoxin 6 from chinese mitten crab eriocheir sinensis. | peroxiredoxin is a superfamily of antioxidative proteins that play important roles in protecting organisms against the toxicity of reactive oxygen species (ros). in this study, the full-length cdna encoding peroxiredoxin 6 (designated esprx6) was cloned from chinese mitten crab eriocheir sinensis by using rapid amplification of cdna ends (race) approaches. the full-length cdna of esprx6 was of 1076 bp, containing a 5' untranslated region (utr) of 69 bp, a 3' utr of 347 bp with a poly (a) tail, a ... | 2009 | 18992822 |
| mitochondrial dna-inferred population structure and demographic history of the mitten crab (eriocheir sensu stricto) found along the coast of mainland china. | the mitten crab, eriocheir sensu stricto, is economically important in east asia, although it is an invasive species in europe and north america. little is known about its population structure and historical demography in its native range, especially along the pacific coast of china. we collected mitochondrial cytochrome oxidase subunit ii and cytochrome b sequences from 154 individuals distributed in the rivers along the chinese coast and 15 individuals from japan. phylogenetic analysis resulte ... | 2008 | 19160479 |
| prevalence and potential pathogenicity of vibrio parahaemolyticus in chinese mitten crabs (eriocheir sinensis) harvested from the river thames estuary, england. | chinese mitten crabs (eriocheir sinensis) have been described as an alien invasive species in the river thames, united kingdom, and elsewhere in europe. the crabs can cause considerable physical damage to the riverbeds and threaten native ecosystems. trapping has been considered an option, but such attempts to control mitten crab populations in germany in the 1930s failed. in the united kingdom, it has been suggested that commercial exploitation of the species could be employed as a control opti ... | 2009 | 19205465 |
| a thioredoxin with antioxidant activity identified from eriocheir sinensis. | thioredoxin, with a redox-active disulfide/dithiol in the active site, is the major ubiquitous disulfide reductase responsible for maintaining proteins in their reduced state. in the present study, the cdna encoding thioredoxin-1 (designated estrx1) was cloned from chinese mitten crab eriocheir sinensis by using rapid amplification of cdna ends (race) approaches. the full-length cdna of estrx1 was of 641 bp, containing a 5' untranslated region (utr) of 17 bp, a 3' utr of 306 bp with a poly (a) t ... | 2009 | 19269333 |
| dioxin and pcb contamination in chinese mitten crabs: human consumption as a control mechanism for an invasive species. | the chinese mitten crab eriocheir sinensis is an invasive species in north american and northeastern european rivers and estuaries, especially the thames, england, with the potential to cause considerable ecological and structural environmental damage. the brown meat of sexually ripe mitten crabs is highly prized in far eastern restaurants, suggesting that harvesting for culinary purposes offers a potential population control mechanism. we have analyzed tissues of thames and dutch mitten crabs f ... | 2009 | 19350945 |
| chance establishment for sexual, semelparous species: overcoming the allee effect. | we formalize the establishment process for a sexual, semelparous organism through the use of hierarchical probability modeling from parameters of survival, probability of being female, probability of being fertilized, and expected fecundity. we show how to calculate the expected per capita growth rate and probability of extinction. an allee effect is observed if the expected population growth rate decreases as the initial population size decreases. the model can be further extended as a stochast ... | 2009 | 19368522 |
| molecular cloning and characterization of the lipopolysaccharide and beta-1, 3-glucan binding protein in chinese mitten crab (eriocheir sinensis). | the lipopolysaccharide and beta-1, 3-glucan binding protein (lgbp) is a pattern recognition protein which is fundamental for the innate immune response of crustaceans. a partial cdna produced by the random sequencing of cdna clones from a haemocyte cdna library of eriocheir sinensis showed similarity to the lgbp gene of the chinese shrimp (fenneropenaeus chinensis). the full-length cdna was subsequently cloned and sequenced by the technique of rapid amplification of cdna ends (race). the e. sine ... | 2009 | 19426826 |
| two thymosin-repeated molecules with structural and functional diversity coexist in chinese mitten crab eriocheir sinensis. | recently, beta-thymosin-like proteins with multiple thymosin domains (defined as thymosin-repeated proteins) have been identified from invertebrate. in the present study, the cdnas of two thymosin-repeated proteins (designated estrp1 and estrp2) were cloned from chinese mitten crab by expressed sequence tags (est) techniques. blast analysis presented three and two thymosin domains in estrp1 and estrp2, respectively, with the identities amongst the five domains varying from 47% to 100%. both estr ... | 2009 | 19428488 |
| use of oxytetracycline for the treatment of tremor disease in the chinese mitten crab eriocheir sinensis. | the causative agent of tremor disease (td) in the chinese mitten crab eriocheir sinensis has been shown to be a member of the genus spiroplasma. in the present study, a susceptibility test indicated that oxytetracycline (otc) has both a high degree of efficacy in the inhibition of spiroplasma and a broad range of safe concentrations. treatment experiments showed that the best concentration of otc for use against td was 40 mg otc kg(-1) crab weight. acute toxicity experiments demonstrated that th ... | 2009 | 19565702 |
| the involvement of suppressors of cytokine signaling 2 (socs2) in immune defense responses of chinese mitten crab eriocheir sinensis. | the suppressors of cytokine signaling 2 (socs2) has been identified as negative feedback inhibitors for various cytokines signaling via the jak/stat pathway. in the present studies, the cdna of eriocheir sinensis socs2 (designated as essocs2) was cloned by expressed sequence tag (est) and rapid amplification of cdna ends (race) approaches. the full-length cdna of essocs2 was of 2535bp, consisting of an open reading frame (a orf) of 1071bp encoding a polypeptide of 357 amino acids. the deduced am ... | 2010 | 19686773 |
| a clip domain serine protease (csp) from the chinese mitten crab eriocheir sinensis: cdna characterization and mrna expression. | clip domain serine protease (csp), characterized by conserved clip domains, is a new serine protease family identified mainly in arthropod, and plays important roles in development and immunity. in the present study, the full-length cdna of a csp (designated escsp) was cloned from chinese mitten crab eriocheir sinensis by expressed sequence tags (ests) and pcr techniques. the 1380 bp escsp cdna contained a 1152 bp open reading frame (orf) encoding a putative csp of 383 amino acids, a 5'-untransl ... | 2009 | 19699801 |
| characterization of a serine proteinase homologous (sph) in chinese mitten crab eriocheir sinensis. | the serine protease homologous (sph) is an important cofactor of prophenoloxidase-activating enzyme (ppae). the gene of sph of chinese mitten crab eriocheir sinensis (essph) in hemocytes was cloned and characterized using reverse transcript polymerase chain reaction (rt-pcr) and rapid amplification of cdna ends (race). the sph cdna consisted of 1386 bp with an open reading frame (orf) encoded a protein of 378 amino acids, 154 bp 5'-untranslated region, and 95 bp 3'-untranslated region. sequence ... | 2010 | 19720078 |
| the construction of a cdna library enriched for immune genes and the analysis of 7535 ests from chinese mitten crab eriocheir sinensis. | chinese mitten crab eriocheir sinensis is one of the most important aquaculture crustacean species in china. a cdna library was constructed from hemocytes of e. sinensis challenged with the mixture of listonella anguillarum and staphylococcus aureus, and randomly sequenced to collect genomic information and identify genes involved in immune defense response. single-pass 5' sequencing of 10368 clones yielded 7535 high quality ests (expressed sequence tags) and these ests were assembled into 2943 ... | 2009 | 19748587 |
| a putative endocrine factor sibd (single insulin binding domain protein) involved in immune response of chinese mitten crab eriocheir sinensis. | insulin growth factor binding proteins (igfbps), characterized by the conserved insulin binding (ib) domains, are important components of endocrine system and play key roles in metabolism and growth. in the present study, the full-length cdna of a single ib domain protein (designated essibd) was identified from chinese mitten crab eriocheir sinensis based on expressed sequence tag (est) sequence. the 1187 bp essibd cdna contained a 321 bp open reading frame (orf) encoding a putative protein of 1 ... | 2010 | 19772924 |
| two novel secreted ferritins involved in immune defense of chinese mitten crab eriocheir sinensis. | as a principal extracellular iron storage molecule, secreted ferritin plays an important role in the iron-withholding strategy of innate immunity. in this study, two novel secreted ferritins were identified from chinese mitten crab eriocheir sinensis (designated as esfer-1 and esfer-2) by rapid amplification of cdna ends (race) approaches and expressed sequence tag (est) analysis. the full-length cdnas of esfer-1 and esfer-2 were of 1278 and 1595 bp, respectively, both containing a putative iron ... | 2010 | 20045469 |
| molecular characterization and expression of a crustin-like gene from chinese mitten crab, eriocheir sinensis. | crustin is a cysteine-rich antibacterial peptide which widely distributes within decapod crustaceans. in the present study, the cdna encoding crustin-like peptide (designated cruses) was cloned from chinese mitten crab eriocheir sinensis by using rapid amplification of cdna ends (race) approaches and expressed sequence tag analysis. the full-length cdna of cruses was of 796bp, containing a 5' untranslated region (utr) of 214bp, a 3' utr of 267bp with a poly(a) tail, and an open reading frame (or ... | 2010 | 20144896 |
| identification and characterization of spiralin-like protein slp25 from spiroplasma eriocheiris. | spiroplasma eriocheiris causes tremor disease (td) of chinese mitten crab eriocheir sinensis, but little is known about the molecular characterization of this pathogen. the present study was undertaken to identify a unique gene of spiroplasma, spiralin-like protein (slp25) and analyze its character. the full-length dna of slp25 is 699 bp encodes a protein of 232 amino acid residues. the theoretical isoelectric point and molecular weight for the slp25 are 4.50 and 24.67 kda, respectively. the sim ... | 2010 | 20153936 |
| molecular cloning and characterization of alpha 2-macroglobulin (alpha2-m) from the haemocytes of chinese mitten crab eriocheir sinensis. | alpha 2-macroglobulin (alpha2-m) is a large protein in blood and is able to inactivate a variety of proteinases in invertebrates and vertebrates. the alpha2-m gene was obtained from the chinese mitten crab, eriocheir sinensis, by the reverse transcript-polymerase chain reaction (rt-pcr) and the rapid amplification of cdna ends (race). the alpha2-m cdna of e. sinensis contained 4851 bp with an open reading frame of 4371 bp, a 70 bp 5'-untranslated region, a 410 bp 3'-untranslated region, and thre ... | 2010 | 20394825 |
| the second anti-lipopolysaccharide factor (esalf-2) with antimicrobial activity from eriocheir sinensis. | the anti-lipopolysaccharide factor (alf) is a small basic protein that can bind and neutralize lipopolysaccharide (lps), mediating degranulation and activation of an intracellular coagulation cascade. in the present study, cdna of the second eriocheir sinensis alf (designated as esalf-2) was cloned and the full-length cdna of esalf-2 was of 724bp, consisting of an open reading frame (orf) of 363bp encoding a polypeptide of 120 amino acids. the deduced amino acid of esalf-2 shared 82% similarity ... | 2010 | 20416335 |
| spiroplasma eriocheiris sp. nov., associated with mortality in the chinese mitten crab, eriocheir sinensis. | a motile bacterium, designated strain tda-040725-5(t), was isolated from the haemolymph of a chinese mitten crab, eriocheir sinensis, with tremor disease. based on 16s rrna gene sequence analysis, the strain was phylogenetically distinct from other spiroplasmas but was closely related to spiroplasma mirum atcc 29335(t). cells of strain tda-040725-5(t) were variable in length and shape, helical and motile, as determined by phase-contrast light microscopy. examination by electron microscopy reveal ... | 2011 | 20418415 |
| spiralin-like protein slp31 from spiroplasma eriocheiris as a potential antigen for immunodiagnostics of tremor disease in chinese mitten crab eriocheir sinensis. | spiroplasma eriocheiris caused massive mortality of chinese mitten crab eriocheir sinensis but little is known about the molecular characteristics of this microorganism. we described here the identification of a spiralin-like protein (slp31) from s. eriocheiris and expression in escherichia coli. analysis of the nucleotide sequence revealed that the clone has an open reading frame of 837 bp encoding a protein of 279 amino acids. theoretical isoelectric point and molar mass for slp31 are 7.72 and ... | 2010 | 20526837 |
| a delta-class glutathione transferase from the chinese mitten crab eriocheir sinensis: cdna cloning, characterization and mrna expression. | glutathione-s-transferases (gsts), a major superfamily of antioxidative enzymes, play an important role in protecting organisms against the toxicity of reactive oxygen species (ros). the delta-class gst cdna was cloned and sequenced by rapid amplification of cdna ends (race) from the chinese mitten crab, eriocheir sinensis. the 938 bp e. sinensis gst gene, encoding a polypeptide of 216 amino acids, showed significant similarity to homologous genes in insects. the deduced amino acid sequence of t ... | 2010 | 20538061 |
| identification of adhesin-like protein alp41 from spiroplasma eriocheiris and induction immune response of eriocheir sinensis. | spiroplasma eriocheiris is a causative agent of the tremor disease (td) of chinese mitten crab eriocheir sinensis which is a novel pathogen of aquatic animals found in recent years. a gene, adhesin-like protein (alp41), of s. eriocheiris from e. sinensis was identified and its characteristics were analyzed in present paper. the role of this pathogen's host-binding protein in promoting immune responses was characterized through analyzing the interaction between s. eriocheiris and e. sinensis. the ... | 2010 | 20538062 |
| molecular cloning and characterization of kifc1-like kinesin gene (es-kifc1) in the testis of the chinese mitten crab eriocheir sinensis. | kifc1 is essential for acrosome biogenesis and nuclear reshaping during the spermiogenesis of mammals. to explore its functions during the same process in the chinese mitten crab eriocheir sinensis, we have cloned and sequenced the cdna of a mammalian kifc1 homologue (termed es-kifc1) from the total rna of the testis. the 2340bp es-kifc1 cdna contained a 102bp 5' untranslated region, a 117bp 3' untranslated region and a 2121bp open reading frame. the putative es-kifc1 protein owns the divergent ... | 2010 | 20554056 |
| identification and characterization of a cystatin gene from chinese mitten crab eriocheir sinensis. | cystatins are a superfamily of proteins as reversible inhibitor of cysteine proteinases which play essential roles in a spectrum of physiological and immunological processes. in this study, a novel member of cystatin superfamily was identified from chinese mitten crab eriocheir sinensis (designated escystatin) by expressed sequence tag (est) analysis and rapid amplification of cdna ends (race) approaches. the full-length cdna of escystatin was of 1486 bp, consisting of a 5'-terminal untranslated ... | 2010 | 20570735 |
| molecular cloning, characterization and expression analysis of cathepsin c gene involved in the antibacterial response in chinese mitten crab, eriocheir sinensis. | cathepsins, a superfamily of hydrolytic enzymes produced and enclosed within lysosomes, function in immune response in vertebrates; however, their function within the innate immune system of invertebrates remains largely unknown. therefore, we investigated the immune functionality of cathepsin c (catc) in chinese mitten crab (eriocheir sinensis), a commercially important and disease vulnerable aquaculture species. the full-length catc cdna (1481 bp) was cloned via pcr based upon an initial expre ... | 2010 | 20600276 |
| molecular cloning and characterization of a serine proteinase homolog prophenoloxidase-activating factor in the swimming crab portunus trituberculatus. | serine proteinase homologues (sphs), as one of prophenoloxiase-activating factors (ppafs), play critical roles in innate immunity of crabs. based on an est from the eyestalk full length cdna library, the complete cdna (designated as ptsph) and genomic dna of sph from the swimming crab portunus trituberculatus were cloned in this study. the estimated molecular weight of mature ptsph (354 amino acids) was 38.7 kda and its isoelectric point was 5.08. multiple sequence alignment revealed that ptsph ... | 2010 | 20633654 |
| molecular cloning and expression of a relish gene in chinese mitten crab eriocheir sinensis. | nf-κb is a b-cell specific transcription factor that plays crucial roles in inflammation, immunity, apoptosis, development and differentiation. in the present study, a novel nf-κb-like transcription factor relish was cloned from chinese mitten crab eriocheir sinensis (designated as esrelish) by rapid amplification of cdna ends (race) technique based on expressed sequence tag (est). the full-length cdna of esrelish was of 5034 bp, consisting of a 5' untranslated region (utr) of 57 bp, a 3' utr of ... | 2010 | 20670337 |
| molecular cloning, characterization, expression and activity analysis of cathepsin l in chinese mitten crab, eriocheir sinensis. | cathepsins, a superfamily of hydrolytic enzymes produced and enclosed within lysosomes, function in immune response in vertebrates; however, their function within the innate immune system of invertebrates remains largely unknown. therefore, we investigated the immune functionality of cathepsin l (catl) in chinese mitten crab (eriocheir sinensis), a commercially important and disease vulnerable aquaculture species. the full-length catl cdna (1274 bp) was cloned via pcr based upon an initial expre ... | 2010 | 20732429 |
| molecular cloning, characterization and expression analysis of cathepsin a gene in chinese mitten crab, eriocheir sinensis. | cathepsins, a superfamily of hydrolytic enzymes produced and enclosed within lysosomes, function in immune response in vertebrates; however, their function within the innate immune system of invertebrates remains largely unknown. therefore, we investigated the immune functionality of cathepsin a (cata) in chinese mitten crab (eriocheir sinensis), a commercially important and disease vulnerable aquaculture species. the full length cata cdna (2200 bp) was cloned via pcr based upon an initial expre ... | 2011 | 20817057 |
| molecular cloning, genomic organization and antibacterial activity of a second isoform of antilipopolysaccharide factor (alf) from the mud crab, scylla paramamosain. | antimicrobial peptides (amps) serve a major role in host defense systems against microbial invasion. in this study, a novel isoform (alfsp2) of antilipopolysaccharide factors (alfs) was cloned from the mud crab, scylla paramamosain. the open reading frame of the alfsp2 cdna is 348 bp and encodes for a predicted 115 amino acid residues (12.92 kda), and a mature protein of 94 amino acids and a molecular mass of 10.79 kda. the amino acid sequence of alfsp2 has an overall similarity of 74%, 66% and ... | 2010 | 20883796 |
| molecular cloning, characterization and expression analysis of two apoptosis genes, caspase and nm23, involved in the antibacterial response in chinese mitten crab, eriocheir sinensis. | apoptosis is a central regulatory feature of the immune system, and the most common form of death among immunological cells. however, the function of apoptosis, within the innate immune system of invertebrates, remains largely unknown. for this reason, we investigated the immune functionality of two apoptosis genes, caspase and nm23, in the chinese mitten crab (eriocheir sinensis), which is a commercially important and disease vulnerable aquaculture species. the entire length caspase and nm23 cd ... | 2010 | 21050890 |
| molecular cloning, characterization and expression analysis of macrophage migration inhibitory protein (mif) in chinese mitten crab, eriocheir sinensis. | macrophage migration inhibitory factor (mif) as a multi-functional cytokine mediating both innate and adaptive immune responses, however, their function within the innate immune system of invertebrates remains largely unknown. therefore, we investigated the immune functionality of mif in chinese mitten crab (eriocheir sinensis), a commercially important and disease vulnerable aquaculture species. the full-length mif cdna (704 bp) was cloned via pcr based upon an initial expressed sequence tag (e ... | 2010 | 21087669 |
| characterization of two c-type lectin-like domain (ctld)-containing proteins from the cdna library of chinese mitten crab eriocheir sinensis. | chinese mitten crab eriocheir sinensis is one of the most important aquaculture crustacean species in china. a cdna library was constructed from mixed tissues of e. sinensis challenged with lps. eight genes involved in immune response were identified from 319 single colonies. among them, two different c-type lectin-like domain (ctld)-containing proteins were firstly identified in chinese mitten crab. the full-length cdna sequences of two c-type lectin-like domain (ctld)-containing proteins named ... | 2010 | 21134464 |
| an interleukin-2 enhancer binding factor 2 homolog involved in immune response from chinese mitten crab eriocheir sinensis. | as a transcription factor, interleukin-2 enhancer binding factor 2 (ilf2) regulates il-2 gene at level of transcription, splicing and translation in vertebrates and plays significant roles in immune system. in this study, an ilf2 homolog was identified from chinese mitten crab eriocheir sinensis (designated as esilf) by expressed sequence tag (est) analysis. the full-length cdna of esilf was of 2159bp, containing a 5' untranslated region (utr) of 90bp, a 3' utr of 866bp with a poly (a) tail, and ... | 2011 | 21439385 |
| a novel type iii crustin (cruses2) identified from chinese mitten crab eriocheir sinensis. | antimicrobial peptides are important effectors in the host innate immune response against microbial invasion. in the present study, the cdna encoding a crustin (designated cruses2) was cloned from chinese mitten crab eriocheir sinensis by using est analysis and rapid amplification of cdna ends (race) approach. the full-length cdna of cruses2 was of 1237 bp, containing a 5' untranslated region (utr) of 12 bp, a 3' utr of 886 bp with a poly (a) tail, and an open reading frame (orf) of 339 bp encod ... | 2011 | 21549196 |
| molecular cloning, characterization and expression of a c-type lectin cdna in chinese mitten crab, eriocheir sinensis. | c-type lectins are pattern-recognition proteins which are functionally important for pathogen recognition and immune regulation in vertebrates and invertebrates. in this study, a lectin cdna named as es-lectin was cloned and characterized from the chinese mitten crab, eriocheir sinensis. the full-length sequence of this es-lectin cdna was 651 bp, including an open reading frame of 483 bp encoding 160 amino acids. the predicted molecular weight of the es-lectin was 11.8 kda. a typical signal pept ... | 2011 | 21669290 |
| a double wap domain-containing protein from chinese mitten crab eriocheir sinensis with antimicrobial activities against gram-negative bacteria and yeast. | the whey acidic protein (wap) domain is characterized by a 'four-disulfide-core' (4-dsc) motif comprising of approximately 50 amino acids with eight highly conserved cysteine residues. previous research indicated that wap domain-containing proteins played an important role in the innate immunity of crustaceans. in the present study, a novel double wap domain (dwd)-containing protein gene was identified from chinese mitten crab eriocheir sinensis (designated esdwd) by expressed sequence tag (est) ... | 2011 | 21798281 |
| molt-inhibiting hormone from chinese mitten crab (eriocheir sinensis): cloning, tissue expression and effects of recombinant peptide on ecdysteroid secretion of yos. | molt-inhibiting hormone (mih), a member of the crustacean hyperglycemic hormone (chh) family, inhibits the synthesis of ecdysteroid in y-organ (yo) and plays a significant role in the regulation of molting and growth of crustaceans. a complete cdna sequence encoding mih (ers-mih, genbank accession no.: dq341280) was cloned from eyestalk of chinese mitten crab (eriocheir sinensis) by 5' and 3' races and pcr cloning. the full-length cdna consists of 1457bp with a 330bp open reading frame, encoding ... | 2011 | 21827759 |
| Identification and comparative analysis of the Eriocheir sinensis microRNA transcriptome response to Spiroplasma eriocheiris infection using a deep sequencing approach. | The Chinese mitten crab Eriocheir sinensis is one of the most important freshwater aquaculture crustacean species in China. MicroRNAs (miRNAs) are small non-coding RNAs that are important effectors in the intricate host-pathogen interaction network. To increase the repertoire of miRNAs characterized in crustaceans and to examine the relationship between host miRNA expression and pathogen infection, we used the Illumina/Solexa deep sequencing technology to sequence two small RNA libraries prepare ... | 2012 | 22166732 |
| two kazal-type protease inhibitors from macrobrachium nipponense and eriocheir sinensis: comparative analysis of structure and activities. | kazal-type inhibitors (kpis) play important roles in many biological and physiological processes, such as blood clotting, the immune response and reproduction. in the present study, two male reproductive tract kpis, termed man-kpi and ers-kpi, were identified in macrobrachium nipponense and eriocheir sinensis, respectively. the inhibitory activities of recombinant man-kpi and ers-kpi against chymotrypsin, elastase, trypsin and thrombin were determined. the results showed that both of them strong ... | 2011 | 22200638 |
| Hepatospora eriocheir (Wang and Chen, 2007) gen. et comb. nov. infecting invasive Chinese mitten crabs (Eriocheir sinensis) in Europe. | We describe a microsporidian parasite infecting non-native Chinese mitten crabs (Eriochier sinensis) from Europe. Electron microscopy revealed merogonic and sporogonic life stages bound within a plasmalemma. The crab parasite develops polar tube precursors at the sporont stage but does not complete formation of the intact spore extrusion apparatus at the stage of the sporogonial plasmodium like Enterocytozoon bienuesi and other representatives of the Enterocytozoonidae. Its presence within an aq ... | 2011 | 21854783 |
| Luna stain, an improved selective stain for detection of microsporidian spores in histologic sections. | Microsporidia in histologic sections are most often diagnosed by observing spores in host tissues. Spores are easy to identify if they occur in large aggregates or xenomas when sections are stained with hematoxylin and eosin (H&E). However, individual spores are not frequently detected in host tissues with conventional H&E staining, particularly if spores are scattered within the tissues, areas of inflammation, or small spores in nuclei (i.e. Nucleospora salmonis). Hence, a variety of selective ... | 2011 | 21848126 |
| Permanent Genetic Resources added to Molecular Ecology Resources Database 1 August 2011-30 September 2011. | This article documents the addition of 299 microsatellite marker loci and nine pairs of single-nucleotide polymorphism (SNP) EPIC primers to the Molecular Ecology Resources (MER) Database. Loci were developed for the following species: Alosa pseudoharengus, Alosa aestivalis, Aphis spiraecola, Argopecten purpuratus, Coreoleuciscus splendidus, Garra gotyla, Hippodamia convergens, Linnaea borealis, Menippe mercenaria, Menippe adina, Parus major, Pinus densiflora, Portunus trituberculatus, Procontar ... | 2012 | 22136175 |
| A ?-aminobutyrate type A receptor-associated protein involved in the immune response of Eriocheir sinensis. | The ?-aminobutyrate type A receptor-associated protein (GABARAP) is a ubiquitin-like modifier, which is implicated in membrane trafficking and fusion events of ?-aminobutyrate type A receptor, autophagy and apoptosis. In the present study, the gene encoding GABARAP (designated EsGABARAP) was cloned from Chinese mitten crab Eriocheir sinensis by using rapid amplification of cDNA ends approach and expression sequence tag (EST) analysis. The full-length cDNA of EsGABARAP was of 457 bp, containing a ... | 2011 | 22008098 |
| [cytochemical observations of hemocytes in decapod brachyuran crustacea (author's transl)]. | hemocytes from two species of crabs, eriocheir sinensis and carcinus maenas, have been submitted to different cytochemical reactions in order to bring out the nature of granules' content, either by specific coloration or be enzymatic digestion. light as well as electron microscopy has been used. the granules appeared to be made essentially of basic proteins, rich in arginin and disulfide bridges but poor in tyrosin. these proteins are linked with nonglycogenic carbohydrates. glycogen deposits of ... | 2002 | 127769 |
| gill-tissue respiration in the crab eriocheir sinensis. | | 2015 | 4451906 |
| similarities between decapod and insect neuropeptidomes. | background. neuropeptides are important regulators of physiological processes and behavior. although they tend to be generally well conserved, recent results using trancriptome sequencing on decapod crustaceans give the impression of significant differences between species, raising the question whether such differences are real or artefacts. methods. the blast+ program was used to find short reads coding neuropeptides and neurohormons in publicly available short read archives. such reads were th ... | 2016 | 27257538 |
| identification, distribution and molecular evolution of the pacifastin gene family in metazoa. | members of the pacifastin family are serine peptidase inhibitors, most of which are produced as multi domain precursor proteins. structural and biochemical characteristics of insect pacifastin-like peptides have been studied intensively, but only one inhibitor has been functionally characterised. recent sequencing projects of metazoan genomes have created an unprecedented opportunity to explore the distribution, evolution and functional diversification of pacifastin genes in the animal kingdom. | 2009 | 19435517 |
| transcriptome changes in eriocheir sinensis megalopae after desalination provide insights into osmoregulation and stress adaption in larvae. | eriocheir sinensis, an extremely invasive alien crab species, has important economic value in china. it encounters different salinities during its life cycle, and at the megalopal stage it faces a turning point regarding the salinity in its environment. we applied rna sequencing to e. sinensis megalopae before (mb) and after (ma) desalination, resulting in the discovery of 21,042 unigenes and 908 differentially expressed genes (degs, 4.32% of the unigenes). the degs primarily belonged to the gen ... | 2014 | 25470496 |
| transcriptome analysis and discovery of genes involved in immune pathways from hepatopancreas of microbial challenged mitten crab eriocheir sinensis. | the chinese mitten crab eriocheir sinensis is an important economic crustacean and has been seriously attacked by various diseases, which requires more and more information for immune relevant genes on genome background. recently, high-throughput rna sequencing (rna-seq) technology provides a powerful and efficient method for transcript analysis and immune gene discovery. | 2013 | 23874555 |
| global analysis of the ovarian microrna transcriptome: implication for mir-2 and mir-133 regulation of oocyte meiosis in the chinese mitten crab, eriocheir sinensis (crustacea:decapoda). | micrornas (mirnas) are small non-coding rna molecules that downregulate gene expression by base pairing to the 3'-untranslated region (utr) of target messenger rnas (mrnas). up to now, rare information for the mirnas is available in decapod crustaceans. our previous studies showed that many mirna-binding sites are present in the 3'-utr of the cyclin b in the chinese mitten crab eriocheir sinensis, suggesting that the translation or post-transcription of the crab cyclin b might be regulated by mi ... | 2014 | 24984770 |
| comparative transcriptome analysis of the accessory sex gland and testis from the chinese mitten crab (eriocheir sinensis). | the accessory sex gland (asg) is an important component of the male reproductive system, which functions to enhance the fertility of spermatozoa during male reproduction. certain proteins secreted by the asg are known to bind to the spermatozoa membrane and affect its function. the asg gene expression profile in chinese mitten crab (eriocheir sinensis) has not been extensively studied, and limited genetic research has been conducted on this species. the advent of high-throughput sequencing techn ... | 2013 | 23342039 |
| a catalog of proteins expressed in the ag secreted fluid during the mature phase of the chinese mitten crabs (eriocheir sinensis). | the accessory gland (ag) is an important component of the male reproductive system of arthropods, its secretions enhance fertility, some ag proteins bind to the spermatozoa and affect its function and properties. here we report the first comprehensive catalog of the ag secreted fluid during the mature phase of the chinese mitten crab (eriocheir sinensis). ag proteins were separated by one-dimensional gel electrophoresis and analyzed by reverse phase high-performance liquid chromatography coupled ... | 2015 | 26305468 |
| two endoplasmic reticulum proteins (calnexin and calreticulin) are involved in innate immunity in chinese mitten crab (eriocheir sinensis). | calnexin (cnx) and calreticulin (crt), which are important chaperones in the endoplasmic reticulum (er), participate in the folding and quality control of client proteins. cnx and crt identified from chinese mitten crab (eriocheir sinensis) are designated as escnx and escrt, respectively. escnx and escrt are expressed in the hemocyte, hepatopancrea, gill, and intestine at the mrna and protein level. immunofluorescence analysis indicated that escnx and escrt are located in the er. moreover, the m ... | 2016 | 27279413 |
| characterization and expression of glutamate dehydrogenase in response to acute salinity stress in the chinese mitten crab, eriocheir sinensis. | glutamate dehydrogenase (gdh) is a key enzyme for the synthesis and catabolism of glutamic acid, proline and alanine, which are important osmolytes in aquatic animals. however, the response of gdh gene expression to salinity alterations has not yet been determined in macro-crustacean species. | 2012 | 22615974 |
| functional analysis of kif3a and kif3b during spermiogenesis of chinese mitten crab eriocheir sinensis. | spermatogenesis represents the transformation process at the level of cellular development. kif3a and kif3b are believed to play some roles in the assembly and maintenance of flagella, intracellular transport of materials including organelles and proteins, and other unknown functions during this process. during spermatogenesis in eriocheir sinensis, if the sperm shaping machinery is dependent on kif3a and kif3b remains unknown. | 2014 | 24870586 |
| molecular cloning and gene expression analysis of the leptin receptor in the chinese mitten crab eriocheir sinensis. | leptin is an adipocyte-derived hormone with multiple functions that regulates energy homeostasis and reproductive functions. increased knowledge of leptin receptor function will enhance our understanding of the physiological roles of leptin in animals. | 2010 | 20567508 |
| fatty acid binding proteins fabp9 and fabp10 participate in antibacterial responses in chinese mitten crab, eriocheir sinensis. | invertebrates rely solely on the innate immune system for defense against pathogens and other stimuli. fatty acid binding proteins (fabp), members of the lipid binding proteins superfamily, play a crucial role in fatty acid transport and lipid metabolism and are also involved in gene expression induced by fatty acids. in the vertebrate immune system, fabp is involved in inflammation regulated by fatty acids through its interaction with peroxidase proliferator activate receptors (ppars). however, ... | 2013 | 23365646 |
| comparative transcriptome analysis reveals sex-biased gene expression in juvenile chinese mitten crab eriocheir sinensis. | sex-biased genes are considered to account for most of phenotypic differences between males and females. in order to explore the sex-biased gene expression in crab, we performed the whole-body transcriptome analysis in male and female juveniles of the chinese mitten crab eriocheir sinensis using next-generation sequencing technology. of the 23,349 annotated unigenes, 148 were identified as sex-related genes. a total of 29 candidate genes involved in primary sex determination pathways were detect ... | 2015 | 26193085 |
| glycogen synthase kinase-3 (gsk3) regulates tnf production and haemocyte phagocytosis in the immune response of chinese mitten crab eriocheir sinensis. | glycogen synthase kinase-3 (gsk3) is a serine/threonine protein kinase firstly identified as a regulator of glycogen synthesis. recently, it has been proved to be a key regulator of the immune reaction. in the present study, a gsk3 homolog gene (designated as esgsk3) was cloned from chinese mitten crab, eriocheir sinensis. the open reading frame (orf) was 1824 bp, which encoded a predicted polypeptide of 607 amino acids. there was a conserved serine/threonine kinase domain and a dna binding doma ... | 2017 | 28363635 |
| the receptor for activated c kinase 1 (rack1) functions in hematopoiesis through jnk activation in chinese mitten crab eriocheir sinensis. | receptor for activated c kinase 1 (rack1) is a wd-domain repeating protein which involves in the mediation of various biological processes, including innate immune response. in the present study, a rack1 (designed as esrack1) gene from chinese mitten crab e. sinensis was cloned by rapid amplification of cdna ends (race) technique. the full-length cdna sequence of esrack1 was of 1117 bp with an open reading frame (orf) of 957 bp encoding a polypeptide of 318 amino acids containing seven wd repeat ... | 2016 | 27542616 |
| dietary nano-selenium relieves hypoxia stress and, improves immunity and disease resistance in the chinese mitten crab (eriocheir sinensis). | hypoxia is a relevant physiological challenge for crab culture, and the hemolymph plays a crucial role in response to the hypoxia. in a 60 d feeding trial, chinese mitten crabs (eriocheir sinensis) fed a diet containing 0.2 mg/kg nano-selenium (nanose) showed a significantly increased weight gain rate (wgr) and a reduced feed coefficient (fc) compared to those fed diets with 0, 0.1, 0.4, 0.8, and 1.6 mg/kg nanose. another 90 d feeding trial was conducted to determine the influence of dietary nan ... | 2016 | 27153751 |
| identification, characterization and expression analysis of erk2 in chinese mitten crab eriocheir sinensis after challenge with lps and aeromonas hydrophila. | farming of eriocheir sinensis was seriously threaten by the infection of opportunistic pathogens, especially the gram-negative bacterium. in this paper, we analyzed the sequence of extracellular signal-regulated kinases 2 (erk2) of e. sinensis (eserk2) and its expression levels after challenge with lps and aeromonas hydrophila in both in vivo and in vitro examination. the full-length cdna sequence of eserk2 was 2455 bp in size with an open reading frame (orf) of 1095 bp, encoding the protein of ... | 2016 | 27018024 |
| the characterization of hematopoietic tissue in adult chinese mitten crab eriocheir sinensis. | invertebrates rely on the efficient innate immune mechanisms against invaders, in which the continuous production of hemocytes (hematopoiesis) is indispensable. in the present study, the hematopoietic tissue (hpt) from chinese mitten crab eriocheir sinensis was identified and characterized. it was a thin and non-transparent sheet located at the dorsolateral side of the stomach, which was composed of a series of ovoid lobules. each lobule was surrounded by connective tissue containing a large amo ... | 2016 | 26868307 |
| identification, characterization, and functional studies of a pelle gene in the chinese mitten crab, eriocheir sinensis. | the toll-like receptor/nf-κb signaling pathways play an important role in the innate immune system. in the present study, one pelle gene (named espelle) was identified for the first time from the chinese mitten crab eriocheir sinensis. the full-length cdna of espelle is 3797 bp with a 3156 bp-long open reading frame that encodes a 1051 amino acid protein. espelle protein contains a death domain at the n-terminal and a serine/threonine kinase domain at the c-terminal. a neighbor joining phylogene ... | 2015 | 26026692 |
| a novel integrin function in innate immunity from chinese mitten crab (eriocheir sinensis). | integrins belong to a superfamily of conserved α β heterodimeric cell surface receptors that have critical function in cell migration, differentiation, and survival. in this study, an integrin called esintegrin was identified from chinese mitten crab eriocheir sinensis. esintegrin cdna is 4415 bp long with a 2457 bp open reading frame that encodes an 818 amino acid protein. esintegrin contains a signal peptide, an integrin beta subunit (n-terminal portion of extracellular region) inb domain, an ... | 2015 | 26004499 |
| immune response of peroxinectin of chinese mitten crab eriocheir sinensis to exterior stimulation. | peroxinectin possesses the features of both peroxidase activity and adhesive property and plays important roles in innate immune system of crustaceans. in this study, the sequence of peroxinectin of eriocheir sinensis (espx) was analyzed and its expression in response to exterior stimulation was detected in both in vivo and in vitro examination. we showed that the full-length cdna sequence was composed of 2701 bp and owned a molecular mass of 85.2 kda and a theoretical pi (isoelectric point) of ... | 2015 | 25743380 |
| identification and molecular characterization of a peritrophin-like gene, involved in the antibacterial response in chinese mitten crab, eriocheir sinensis. | peritrophin was first isolated from insect peritrophic membrane (pm) and was thought to protect insects from invasion of microorganisms and to stimulate digestion of food. in this study, a peritrophin-like gene (espt) was obtained from eriocheir sinensis. the full length cdna of espt was 1232 bp, which contained 1005 bp orf encoding a protein of 334 amino acids, including a 22 amino acid signal peptide, and 3 conserved chitin binding type 2 domains (chtbd2) characterized by having a 6-cysteine m ... | 2015 | 25596425 |
| molecular characterization of a cytosolic manganese superoxide dismutase from the chinese mitten crab, eriocheir sinensis. | a cytosolic manganese superoxide dismutase gene (es-cmnsod) was cloned from the chinese mitten crab eriocheir sinensis, using reverse transcription-polymerase chain reaction and the rapid amplification of cdna ends. the open reading frame of es-cmnsod is 867 bp in length and encodes a 288-amino acid protein without a signal peptide. the calculated molecular mass of the translated protein of es-cmnsod is 31.43 kda, with an estimated isoelectric point of 6.30. the deduced amino acid sequence of es ... | 2014 | 25501153 |
| novel myeloid differentiation factor 88, esmyd88, exhibits estube-binding activity in chinese mitten crab eriocheir sinensis. | myeloid differentiation factor 88 (myd88) is a universal and essential adapter protein that participates in the activation of the toll-like receptor/interleukin-1 receptor-mediated signaling pathway. in the present study, a new myd88 gene (named esmyd88) was identified in the chinese mitten crab eriocheir sinensis. the cdna of esmyd88 was 2210 bp long with a 1416 bp open reading frame that encoded a protein with 472 amino acids. predicted esmyd88 protein had a death domain at the n-terminal and ... | 2014 | 25150191 |
| classification and phagocytosis of circulating haemocytes in chinese mitten crab (eriocheir sinensis) and the effect of extrinsic stimulation on circulating haemocytes in vivo. | eriocheir sinensis (henri milne edwards 1854) is one of the most important aquaculture species in china. in this investigation, we characterised the different types of haemocytes of e. sinensis using light and electron microscopy combined with cytochemical analysis and determined the in vivo phagocytic ability of different haemocyte types by injecting polystyrene beads. the haemocytes of e. sinensis were divided into three types: hyalinocytes, semigranulocytes and granulocytes. the hyalinocytes ... | 2014 | 24929244 |
| cloning and characterization of two different l-type lectin genes from the chinese mitten crab eriocheir sinensis. | l-type lectins contain a leguminous lectin domain and bind to high-mannose type oligosaccharides. in the secretory pathway, l-type lectins play crucial functions in the trafficking, sorting, and targeting of maturing glycoproteins. this study identified two novel l-type lectins, designated as esergic-53 and esvip36, from the chinese mitten crab eriocheir sinensis. the complete nucleotide sequence of ergic-53 cdna was 1955 bp, containing a 1506 bp open reading frame (orf) encoding a putative prot ... | 2014 | 24796868 |
| function of two novel single-crd containing c-type lectins in innate immunity from eriocheir sinensis. | c-type lectin is one of the pattern-recognition proteins of the non-self-innate immune system in invertebrates. in this study, two novel c-type lectin cdnas (esctl1 and esctl2) of eriocheir sinensis were cloned and characterized. esctl1 has 169 amino acids, whereas esctl2 has 164 amino acids. these two lectins contain one carbohydrate-recognition domain. phylogenetic analysis showed that esctl1 and esctl2 were not clustered with other reported lectins from crabs. esctl1 and esctl2 were expressed ... | 2014 | 24561128 |
| an ldla domain-containing c-type lectin is involved in the innate immunity of eriocheir sinensis. | c-type lectins (ctls) have crucial functions in recognizing and eliminating pathogens in innate immunity. this study identified a novel low-density lipoprotein receptor class a (ldla) domain-containing ctl, designated as esctldcp, from the chinese mitten crab eriocheir sinensis. the esctldcp cdna is 1258 bp long, with a 975 bp open reading frame that encodes a 324-amino acid protein. esctldcp contains a signal peptide, an ldla, and a single c-type lectin-like domain. esctldcp was only expressed ... | 2014 | 24140432 |
| a double wap domain-containing protein es-dwd1 from eriocheir sinensis exhibits antimicrobial and proteinase inhibitory activities. | whey acidic proteins (wap) belong to a large gene family of antibacterial peptides, which are critical in the host immune response against microbial invasion. the common feature of these proteins is a single wap domain maintained by at least one four-disulfide core (4-dsc) structure rich in cysteine residues. in this study, a double wap domain (dwd)-containing protein, es-dwd1, was first cloned from the chinese mitten crab (eriocheirsinensis). the full-length es-dwd1cdna was 1193 bp, including a ... | 2013 | 23967346 |