| identification and population structure of burkholderia stabilis sp. nov. (formerly burkholderia cepacia genomovar iv). | the burkholderia cepacia complex currently comprises five genomic species, i.e., b. cepacia genomovar i, b. multivorans (formerly known as b. cepacia genomovar ii), b. cepacia genomovar iii, b. cepacia genomovar iv, and b. vietnamiensis (also known as b. cepacia genomovar v). in the absence of straightforward diagnostic tests for the identification of b. cepacia genomovars i, iii, and iv, the last two genomic species were not formally classified as novel burkholderia species (genomovar i contain ... | 2000 | 10698993 |
| dna-based diagnostic approaches for identification of burkholderia cepacia complex, burkholderia vietnamiensis, burkholderia multivorans, burkholderia stabilis, and burkholderia cepacia genomovars i and iii. | bacteria of the burkholderia cepacia complex consist of five discrete genomic species, including genomovars i and iii and three new species: burkholderia multivorans (formerly genomovar ii), burkholderia stabilis (formerly genomovar iv), and burkholderia vietnamiensis (formerly genomovar v). strains of all five genomovars are capable of causing opportunistic human infection, and microbiological identification of these closely related species is difficult. the 16s rrna gene (16s rdna) and reca ge ... | 2000 | 10970351 |
| detection of bacterial virulence genes by subtractive hybridization: identification of capsular polysaccharide of burkholderia pseudomallei as a major virulence determinant. | burkholderia pseudomallei, the etiologic agent of melioidosis, is responsible for a broad spectrum of illnesses in humans and animals particularly in southeast asia and northern australia, where it is endemic. burkholderia thailandensis is a nonpathogenic environmental organism closely related to b. pseudomallei. subtractive hybridization was carried out between these two species to identify genes encoding virulence determinants in b. pseudomallei. screening of the subtraction library revealed a ... | 2001 | 11119486 |
| phenotypic methods for determining genomovar status of the burkholderia cepacia complex. | recent taxonomic advances have demonstrated that burkholderia cepacia is a cluster of at least seven closely related genomic species (or genomovars) collectively referred to as the b. cepacia complex, all of which may cause infections among cystic fibrosis patients and other vulnerable individuals. thus, it is important for clinical microbiologists to be able to differentiate genomovars. prior to this study, 361 b. cepacia complex isolates and 51 isolates easily confused with b. cepacia complex ... | 2001 | 11230429 |
| burkholderia cepacia genomovar vi, a new member of the burkholderia cepacia complex isolated from cystic fibrosis patients. | a polyphasic taxonomic study was performed on 23 strains isolated from cystic fibrosis (cf) patients in the usa. these strains were tentatively identified as burkholderia cepacia, burkholderia vietnamiensis and burkholderia or ralstonia sp. using biochemical tests and 16s rdna-based pcr assays. visual comparison of protein profiles indicated that they belonged to a single new group ('group 13'). the polyphasic taxonomic data showed that 18 of these strains represent a new member of the b. cepaci ... | 2001 | 11321071 |
| [burkholderia cepacia: dangers of a phytopathogen organism for patients with cystic fibrosis]. | burkholderia cepacia is an environmental bacterium, capable of colonising vegetal and animal tissues, involved in human opportunist nosocomial infections, and above all, in pulmonary colonisations in patients with cystic fibrosis. in these patients, infection may be followed by a severe deterioration with bacteraemia, leading to death. moreover, owing to the epidemic spread of some clones within cystic fibrosis communities, strict preventive guidelines have to be instituted. early detection of b ... | 2001 | 11397673 |
| burkholderia ambifaria sp. nov., a novel member of the burkholderia cepacia complex including biocontrol and cystic fibrosis-related isolates. | a polyphasic taxonomic study, including amplified fragment length polymorphism (aflp) fingerprinting, dna-dna hybridizations, dna base-ratio determinations, phylogenetic analysis, whole-cell fatty acid analyses and an extensive biochemical characterization, was performed on 19 burkholderia cepacia-like isolates from the environment and cystic fibrosis (cf) patients. several of the environmental isolates have attracted considerable interest due to their biocontrol properties. the polyphasic taxon ... | 2001 | 11491349 |
| pcr-based detection and identification of burkholderia cepacia complex pathogens in sputum from cystic fibrosis patients. | pcr amplification of the reca gene followed by restriction fragment length polymorphism (rflp) analysis was investigated for the rapid detection and identification of burkholderia cepacia complex genomovars directly from sputum. successful amplification of the b. cepacia complex reca gene from cystic fibrosis (cf) patient sputum samples containing b. cepacia genomovar i, burkholderia multivorans, b. cepacia genomovar iii, burkholderia stabilis, and burkholderia vietnamiensis was demonstrated. in ... | 2001 | 11724828 |
| suppression-subtractive hybridisation reveals variations in gene distribution amongst the burkholderia cepacia complex, including the presence in some strains of a genomic island containing putative polysaccharide production genes. | some strains of the burkholderia cepacia complex, including the et12 lineage, have been implicated in epidemic spread amongst cystic fibrosis (cf) patients. suppression-subtractive hybridisation was used to identify genomic regions within strain j2315 (et12 lineage; genomovar iiia) that were absent from a non-transmissible genomovar iiib strain. sequence data from 15 subtracted clones were used to interrogate the genome sequence of strain j2315 and identify genomic regions incorporating the subt ... | 2003 | 12610727 |
| dna-dna hybridization study of burkholderia species using genomic dna macro-array analysis coupled to reverse genome probing. | the present study was aimed at simplifying procedures to delineate species and identify isolates based on dna-dna reassociation. dna macro-arrays harbouring genomic dna of reference strains of several burkholderia species were produced. labelled genomic dna, hybridized to such an array, allowed multiple relative pairwise comparisons. based on the relative dna-dna relatedness values, a complete data matrix was constructed and the ability of the method to discriminate strains belonging to differen ... | 2003 | 12807195 |
| comparative analysis of plant and animal models for characterization of burkholderia cepacia virulence. | a simple alfalfa model was developed as an alternative infection model for virulence studies of the burkholderia cepacia complex. symptoms of disease were observed in wounded alfalfa seedlings within 7 days following inoculation of 10(1) to 10(5) cfu of most strains of the b. cepacia complex. strains from seven genomovars of the b. cepacia complex were tested for virulence in the alfalfa model, and the degree of virulence was generally similar in strains belonging to the same genomovar. strains ... | 2003 | 12933878 |
| epidemiology and clinical course of burkholderia cepacia complex infections, particularly those caused by different burkholderia cenocepacia strains, among patients attending an italian cystic fibrosis center. | in this study, the epidemiology of burkholderia cepacia complex (bcc) recovered from the sputum of 75 patients attending the genoa cystic fibrosis (cf) center at the gaslini children's hospital (genoa, italy) was investigated, and the clinical course of the cf patients infected with the different species and genomovars of bcc was evaluated. all isolates were analyzed for genomovar status by reca gene polymorphism and subsequently random amplified polymorphic dna fingerprinting. burkholderia ceno ... | 2004 | 15070994 |
| epidemiology of burkholderia cepacia complex species recovered from cystic fibrosis patients: issues related to patient segregation. | studies of the prevalence of burkholderia cepacia complex species amongst cystic fibrosis (cf) patients in different geographical regions, and the association between cross-infection and putative transmissibility markers, will further our understanding of these organisms and help to address infection-control issues. in this study, b. cepacia complex isolates from cf patients in different regions of europe were analysed. isolates were examined for b. cepacia complex species and putative transmiss ... | 2004 | 15184539 |
| pcr-based identification and characterization of burkholderia cepacia complex bacteria from clinical and environmental sources. | to study the genotypic identification and characterization of the 119 burkholderia cepacia complex (bcc) strains recovered from clinical and environmental sources in japan and thailand. | 2004 | 15482431 |
| nosocomial bloodstream infections with burkholderia stabilis. | burkholderia stabilis was grown from blood cultures of seven patients presenting with signs and symptoms of septicaemia in the intensive care unit at mersin university hospital, mersin, turkey between july and october 2002. four patients had one b. stabilis-positive blood culture, two patients had two, and one patient had four. isolates from six of seven patients had the same resistotype and random amplified polymorphic dna analysis type. despite treatment with ciprofloxacin and imipenem, to whi ... | 2005 | 15571853 |
| use of suppression-subtractive hybridization to identify genes in the burkholderia cepacia complex that are unique to burkholderia cenocepacia. | we have previously shown differences in virulence between species of the burkholderia cepacia complex using the alfalfa infection model and the rat agar bead chronic infection model. burkholderia cenocepacia strains were more virulent in these two infection models than burkholderia multivorans and burkholderia stabilis strains. in order to identify genes that may account for the increased virulence of b. cenocepacia, suppression-subtractive hybridization was performed between b. cenocepacia k56- ... | 2005 | 16030222 |
| distribution and genomic location of active insertion sequences in the burkholderia cepacia complex. | this study aimed firstly to establish the distribution and copy number within the burkholderia cepacia complex of three insertion sequences (is402, is407 and is1416) that possess the ability to activate transcription and hence influence gene expression. a second aim was to map the genomic insertion sites of one of the active insertion sequences (is407) to establish putative links between insertion site and downstream gene activation. the resulting data revealed that all three insertion sequences ... | 2006 | 16388024 |
| sustained endemicity of burkholderia cepacia complex in a pediatric institution, associated with contaminated ultrasound gel. | to determine whether contaminated ultrasound gel is the source of intermittent outbreaks of nosocomial infection due to burkholderia cepacia complex in patients without cystic fibrosis since 1992. | 2006 | 16622813 |
| identification of burkholderia cepacia complex pathogens by rapid-cycle pcr with fluorescent hybridization probes. | members of the burkholderia cepacia complex are important bacterial pathogens in cystic fibrosis (cf) patients. the b. cepacia complex currently consists of nine genetic subgroups (genomovars) of different epidemiological relevance and possibly of different pathogenic potential in humans. in this study, a new approach was developed for the rapid identification of b. cepacia genomovar i, burkholderia multivorans (genomovar ii), burkholderia cenocepacia (lineage iii-a and iii-b), burkholderia stab ... | 2006 | 16687590 |
| identification of bacteria isolated from diseased neungee mushroom, sarcodon aspratus. | as the first step in an investigation of the problem with quality deterioration seen in the neungee mushroom (sarcodon aspratus) due to bacterial overgrowth during its storage, an attempt to isolate bacterial strains was made using infected gills of sarcodon aspratus. five bacterial strains were isolated; one phototrophic cyanobacterial species and four heterotrophic gram negative rods. the four heterotrophic bacterial isolates (strains p, s, r, and mk1) were subjected to identification based on ... | 2007 | 17304616 |
| hospital outbreak of burkholderia stabilis bacteraemia related to contaminated chlorhexidine in haematological malignancy patients with indwelling catheters. | burkholderia cepacia complex (bcc) is an opportunistic pathogen that occasionally causes hospital outbreaks. this paper describes an outbreak of bcc bacteraemia in haematological malignancy patients related to a contaminated chlorhexidine gluconate solution. eight bcc isolates were obtained from patients hospitalised in the same ward of a cancer centre in a korean hospital. a further three bcc isolates were obtained from 0.5% chlorhexidine gluconate used in the same ward. the isolates were ident ... | 2008 | 18799235 |
| use of fourier transform infrared spectroscopy and chemometrics to discriminate clinical isolates of bacteria of the burkholderia cepacia complex from different species and ribopatterns. | a methodology for the discrimination of burkholderia cepacia complex (bcc) clinical isolates at the species level and at the ribopattern level using fourier transform infrared (ftir) spectroscopy and chemometrics analysis was assessed in this study. different bcc sequential isolates collected at the santa maria hospital (hsm), in portugal, from clinically infected cystic fibrosis (cf) patients were previously classified by established molecular methods at the species level and differentiated at ... | 2009 | 19579020 |
| burkholderia cenocepacia creates an intramacrophage replication niche in zebrafish embryos, followed by bacterial dissemination and establishment of systemic infection. | bacteria belonging to the "burkholderia cepacia complex" (bcc) often cause fatal pulmonary infections in cystic fibrosis patients, yet little is know about the underlying molecular mechanisms. these gram-negative bacteria can adopt an intracellular lifestyle, although their ability to replicate intracellularly has been difficult to demonstrate. here we show that bcc bacteria survive and multiply in macrophages of zebrafish embryos. local dissemination by nonlytic release from infected cells was ... | 2010 | 20086083 |
| diversity analysis of burkholderia cepacia complex in the water bodies of west lake, hangzhou, china. | a survey of burkholderia cepacia complex (bcc) species was conducted in water bodies of west lake in china. a total of 670 bacterial isolates were recovered on selective media. out of them, 39.6% (265 isolates) were assigned to the following species: burkholderia multivorans, burkholderia cenocepacia reca lineage iiia, iiib, burkholderia stabilis, burkholderia vietnamiensis, and burkholderia seminalis while b. cenocepacia is documented as a dominant bcc species in water of west lake. in addition ... | 2011 | 21538256 |
| genomic characterization of jg068, a novel virulent podovirus active against burkholderia cenocepacia. | as is true for many other antibiotic-resistant gram-negative pathogens, members of the burkholderia cepacia complex (bcc) are currently being assessed for their susceptibility to phage therapy as an antimicrobial treatment. the objective of this study was to perform genomic and limited functional characterization of the novel bcc phage jg068 (vb_bcep_jg068). | 2013 | 23978260 |
| complete genome sequences for three chromosomes of the burkholderia stabilis type strain (atcc baa-67). | we report here the complete annotated genome sequence of the burkholderia stabilis type strain atcc baa-67. there were three circular chromosomes with a combined size of 8,527,947 bp and g+c composition of 66.4%. these characteristics closely resemble the genomes of other sequenced members of the burkholderia cepacia complex. | 2016 | 27856590 |
| polyclonal outbreak of bacteremia caused by burkholderia cepacia complex and the presumptive role of ultrasound gel. | a nosocomial polyclonal outbreak associated to bacteremia caused by different burkholderia cepacia complex (bcc) species and clones is reported. molecular characterization identified burkholderia stabilis, burkholderia contaminans, and burkholderia ambifaria among bcc isolates obtained from patients in neonatal and adult intensive care units. bcc was also isolated from an intrinsically contaminated ultrasound gel, which constituted the presumptive bcc source. prior bcc outbreak related to contam ... | 2015 | 26322722 |
| bacterial production of short-chain organic acids and trehalose from levulinic acid: a potential cellulose-derived building block as a feedstock for microbial production. | levulinic acid (la) is a platform chemical derived from cellulosic biomass, and the expansion of la utilization as a feedstock is important for production of a wide variety of chemicals. to investigate the potential of la as a substrate for microbial conversion to chemicals, we isolated and identified la-utilizing bacteria. among the six isolated strains, pseudomonas sp. la18t and rhodococcus hoagie la6w degraded up to 70 g/l la in a high-cell-density system. the maximal accumulation of acetic a ... | 2015 | 25479689 |
| draft genome sequence of burkholderia stabilis la20w, a trehalose producer that uses levulinic acid as a substrate. | burkholderia stabilis la20w produces trehalose using levulinic acid (la) as a substrate. here, we report the 7.97-mb draft genome sequence of b. stabilis la20w, which will be useful in investigations of the enzymes involved in la metabolism and the mechanism of la-induced trehalose production. | 2016 | 27491978 |
| an outbreak of burkholderia stabilis colonization in a nasal ward. | the aim of this study was to describe an outbreak of burkholderia stabilis colonization among patients in a nasal ward. | 2015 | 25578264 |
| modelling and parameter inference of predator-prey dynamics in heterogeneous environments using the direct integral approach. | most bacterial habitats are topographically complex in the micro scale. important examples include the gastrointestinal and tracheal tracts, and the soil. although there are myriad theoretical studies that explore the role of spatial structures on antagonistic interactions (predation, competition) among animals, there are many fewer experimental studies that have explored, validated and quantified their predictions. in this study, we experimentally monitored the temporal dynamic of the predatory ... | 2017 | 28053112 |