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molecular cloning and characterization of a type 3 iodothyronine deiodinase in the pine snake pituophis deppei.the three distinct but related isotypes of the iodothyronine deiodinase family: d1, d2, and d3, have been amply studied in vertebrate homeotherms and to a lesser extent in ectotherms, particularly in reptiles. here, we report the molecular and kinetic characteristics of both the native and the recombinant hepatic d3 from the pine snake pituophis deppei (pdd3). the complete pdd3 cdna (1680 bp) encodes a protein of 287 amino acids (aa), which is the longest type 3 deiodinase so far cloned. pdd3 sh ...201020709065
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