| sarcocystinae: nomina dubia and available names. | examination of the original descriptions of the species of sarcocystis in cattle, sheep, and swine, and of isosporid oocysts shed sporulated by dogs, cats, man, and other carnivores, has shown that it is not possible in most instances to identify unambiguously recently recognized taxa. the original descriptions are insufficient, and because no type specimens exist, could apply to two or more of the presently recognized taxa. we consider the following nomina dubia: sarcocystis hirsuta s. miescher ... | 1979 | 107666 |
| alterations of serum insulin-like growth factor-i (igf-i) and igf-binding proteins (igfbps) in swine infected with the protozoan parasite sarcocystis miescheriana. | the effects of a sarcocystis miescheriana infection on insulin-like growth factor-i (igf-i) and insulin-like growth factor binding proteins (igfbps) were investigated to determine possible mechanisms of growth retardation in growing pigs. sixteen pigs averaging 14 kg body weight were divided into 4 groups of 4 pigs each and infected either with 0.5, 1.0, or 3.0 x 10(6) sporocysts of s. miescheriana. four pigs were retained as non-infected controls; however, they became serologically positive dur ... | 1992 | 1282099 |
| sarcosporidian infection in pigs in uruguay. | examination of enzymatic digests of samples of the crux diaphragm obtained at abbatoirs in montevideo, uruguay, indicated that 57.2% of 269 pigs weighing 90-140 kg were infected with sarcocystis sp. the morphology of sarcocystis in h & e stained sections indicated that they were sarcocystis miescheriana. | 1992 | 1561758 |
| lipid metabolism and sarcocystis miescheriana infection in growing swine. | sixteen 2-month-old pigs were divided into four equal groups and infected with either 500,000, 1,000,000 or 3,000,000 sporocysts of sarcocystis miescheriana. four pigs served as uninfected controls. pigs were bled weekly and serum was collected beginning 14 days prior to infection and continuing until 63 days after infection. body fat composition, as measured by the specific gravity of the carcass, was not affected by infection. there were no significant effects of infection on serum concentrati ... | 1992 | 1615630 |
| prevalence of sarcocystis in domestic pigs in india. | muscle samples from 890 slaughtered pigs were examined for the presence of sarcocysts. a high prevalence rate of 67.98% was observed. two types of microsarcocysts were recorded. the sarcocyst wall of one type had redial striations and the other possessed hair-like villar protrusions. the species were identified as sarcocystis miescheriana and sarcocystis suihominis; there was a slightly higher incidence of the latter species (47.11%) than of the former (43.14%). s. suihominis has been identified ... | 1991 | 1763484 |
| effect of temperature on the infectivity of sarcocystis miescheriana cysts in pork. | sarcocysts of sarcocystis miescheriana in the thigh muscles of pigs became non-infective to pups after heating infected pork in minute pieces at 60 degrees c for 20 min, 70 degrees c for 15 min and 100 degrees c for 5 min. similar pieces of infected muscle tissues, when exposed to -4 degrees c for 2 days or -20 degrees c for 1 day, became non-infective to pups. the experiment suggests that pork containing sarcocysts of s. miescheriana, and possibly of s. suihominis, requires cooking at a minimum ... | 1990 | 2119084 |
| [heart failure in a pig chronically infected with sarcocystis miescheriana--a case report]. | a pig infected with 2 x 10(5) sporocysts of sarcocystis miescheriana which had survived the acute phase of the disease from 12 dpi until 17 dpi retarded in growth and finally died at 60 dpi. from gross pathological examination heart failure was assumed as the cause of death. histopathologically severe myocarditis eosinophilica fibrosa was diagnosed. the sections through the heart muscle contained numerous degenerating and some intact sarcocysts. | 1989 | 2504142 |
| hemostatic alterations in pigs fed sublethal doses of sarcocystis miescheriana. | effects of non-lethal sarcocystis miescheriana infections on the blood coagulation system were investigated. nine pigs were inoculated orally with 2 x 10(5) sporocysts (group a) and nine pigs (group b) served as non-infected controls. blood samples were taken from the vena jugularis externa every 2 or 3 days until 19 days post-infection (dpi). the following parameters were investigated: partial thromboplastin time (ptt), prothrombin time (pt), thrombin time (tt), thrombin coagulase time (tct), f ... | 1989 | 2511658 |
| prostanoids during acute sarcocystiosis in growing pigs. | the stable metabolites of thromboxane a2, prostaglandin e2, and prostaglandin f2 alpha (txb2, pgem, and pgfm, respectively) were measured in the blood plasma of nine castrated male pigs, each inoculated with 2 x 10(5) sporocysts of sarcocystis miescheriana (group a), and in that of nine non-infected controls (group b). all infected pigs developed mild disease, the clinical signs being most severe between days 14 and 17 post infection (p.i.). in the infected pigs of group a, the txb2 plasma level ... | 1989 | 2515535 |
| sarcocystis miescheriana infection and its frequency in pigs in japan. | | 1986 | 3102825 |
| effects of sarcocystis miescheriana infection on carcass weight and meat quality of halothane-tested fattening pigs. | ten halothane-positive pigs (group a) and ten halothane-negative pigs (group c) were each infected per os by 50,000 sporocysts of sarcocystis miescheriana when they had reached a mean body weight of 36 kg. twelve halothane-positive pigs (group b) and ten halothane-negative pigs (group d) served as non-infected controls. thirteen weeks p.i. (post infection), the pigs were slaughtered and the carcass weights and the number of cystozoites in several muscles were determined. in addition, 11 paramete ... | 1987 | 3114937 |
| the effects of sarcocystis miescheriana infections on blood enzymes and weight gain of stress-sensitive and stress-insensitive pigs. | the effects of mild sarcocystis miescheriana infection on blood enzymes and body weight were compared in stress-sensitive (halothane positive) and in stress-insensitive (halothane negative) pigs. ten halothane-positive pigs (group a) and ten halothane-negative pigs (group c) with a mean body weight of 36 kg were each inoculated orally with 50,000 sporocysts of s. miescheriana. twelve halothane-positive pigs (group b) and ten halothane-negative pigs (group d) served as non-infected controls. five ... | 1988 | 3130714 |
| effects of sarcocystis miescheriana infection on carcass quality and on the water-binding capacity of the meat of halothane-tested fattening pigs. | ten halothane-positive pigs (stress sensitive, group a) and ten halothane-negative pigs (stress insensitive, group c) with a mean body weight of 36 kg were each inoculated orally with 50,000 sporocysts of sarcocystis miescheriana. twelve halthane-positive pigs (group b) and ten halothane-negative pigs (group d) served as non-infected controls. thirteen weeks post infection (p.i.) the lean: fat ratios of the pigs of the infected groups a and c were lower (a, 1:0.41 +/- 0.09; c, 1:0.50 +/- 0.10) t ... | 1988 | 3130715 |
| attempted immunization of swine against acute sarcocystosis using cystozoite-derived vaccines. | low-dose sarcocystis miescheriana infections have recently been shown to protect pigs against acute sarcocystosis. because this protective immunity was short-lasting, an alternative immunization strategy was examined. four experimental vaccines were prepared from s. miescheriana cystozoites and tested in 13 pigs. two vaccines were prepared from intact organisms (live and formalin-fixed cystozoites) and 2 from subcellular cystozoite fractions (pellicle and protoplasm extracts). the live vaccine w ... | 1985 | 3919497 |
| [the occurrence of sarcocystis miescheriana in a stock of bacon pigs. an investigation of the frequency of infection during a year (author's transl)]. | | 1974 | 4217444 |
| detection of sarcocystis antigens in the sera of experimentally-infected pigs and mice by an immunoenzymatic assay. | immunoglobulins raised against sarcocystis miescheriana and sarcocystis muris cystozoite antigens were isolated from rabbit immune sera by affinity chromatography (using cnbr-activated sepharose 4b for antigen immobilization). the specific immunoglobulins were incorporated into double-antibody sandwich immuno-enzymatic assays which were firstly quantitated and then used to detect soluble sarcocystis antigens in the sera of experimentally-infected pigs and mice. assays employing immunoglobulins a ... | 1983 | 6407180 |
| [development and duration of immunity against sarcocystis miescheriana in swine under continuous administration of small doses of sporocysts]. | | 1983 | 6409071 |
| persistence of acquired immunity to sarcocystis miescheriana infection in growing pigs. | sixteen 8-week-old pigs were each experimentally immunized by subclinical infections with 10(3) sarcocystis miescheriana (syn. s. suicanis) sporocysts and 8 other pigs served as non-immunized controls. four groups of pigs (each consisting of 4 immunized plus 2 control pigs) were then challenged by infection with 3 x 10(6) sporocysts at either 40, 80, 120 or 160 days post-immunization (dpi) to determine the persistence of the protective immunity against acute sarcocystosis. pigs challenged 40 dpi ... | 1983 | 6419444 |
| class-specific antibody responses in pigs following immunization and challenge with sporocysts of sarcocystis miescheriana. | sixteen pigs were each immunized by oral inoculation with 1000 sporocysts of sarcocystis miescheriana at 8 weeks of age. four equal groups were then challenged with 3 million sporocysts per animal at 40, 80, 120 or 160 days post-immunization (dpi). host antibody responses were monitored using the enzyme-linked immunosorbent assay (elisa) and the indirect fluorescent-antibody test (ifat). when using class-specific elisas, dramatic increases in specific igm-antibodies were observed at 21 dpi and i ... | 1984 | 6440348 |
| [persistence of sarcocystis miescheriana cysts in the muscles of swine following a single or repeated infection]. | | 1984 | 6440529 |
| [dynamics of antibody formation in swine following repeated immunization with sporocysts of sarcocystis miescheriana and a single challenge infection]. | | 1984 | 6441565 |
| prevalence of sarcocystis in sows from iowa. | the hearts of 893 sows from iowa killed for food between january 1990 and february 1992 were examined for sarcocystis bradyzoites by the digestion technique. bradyzoites were found in 163 (18.2%) hearts. sections of formalin-fixed tissues from 28 hearts with demonstrable bradyzoites were examined for sarcocysts. sarcocystis miescheriana sarcocysts were found in six pigs. the prevalence of sarcocystis was apparently not affected by season. | 1994 | 8030181 |
| the time course of the specific antibody response by various elisas in pigs experimentally infected with toxoplasma gondii. | with the aim of developing routine serological tests for monitoring the toxoplasma infection status of danish swine herds, four elisas based on tachyzoite antigen were set up: (1) an indirect elisa for igg-antibody; (2) a blocking elisa for antibody to the membrane antigen, p30; (3) an indirect elisa for igm; (4) a reverse, antibody-catching igm-elisa. groups of pigs (number between 6 and 10) were inoculated with tachyzoites of the rh-strain, tissue cysts of two complete strains, or oocysts in t ... | 1997 | 9231984 |
| validation of the specificity of the modified agglutination test for toxoplasmosis in pigs. | serum samples from toxoplasma gondii-negative pigs infected with protozoa, helminths and viruses, and from fetal pigs were examined for cross reacting antibodies to t. gondii by the use of the modified agglutination test (mat). nonspecific reactions with t. gondii antigen were not found in 1:10 dilutions of sera from eight pigs experimentally infected with sarcocystis miescheriana, 11 pigs infected with ascaris suum, three pigs infected with trichuris suis, ten pigs infected with trichinella spi ... | 1997 | 9299699 |
| pig, donkey and buffalo meat as a source of some coccidian parasites infecting dogs. | experimental infection of dogs with meat samples (oesophagus, heart and diaphragm) from each of 105 pigs, 11 donkeys and 17 egyptian water buffaloes indicated that they contained the infective stages of some coccidian parasites of dogs. the dogs which were fed pig meat shed in their faeces isospora ohioensis, i. canis oocysts and sarcocystis miescheriana sporocysts after prepatent periods of 3-5, 4-7 and 9-10 days, respectively. the dogs which were fed donkey meat excreted only i. ohioensis oocy ... | 1998 | 9760058 |
| sarcocystis miescheriana infection in domestic pigs (sus scrofa) in the philippines. | sarcocystis miescheriana sarcocysts were identified in skeletal muscles of 9 (27%) of 33 swine slaughtered for human consumption. sarcocysts were 144-180 microm x 20-38 microm in size. ultrastructurally, the cyst wall resembled the type 10 sarcocyst wall. the villar protrusions (vp) were 3-4.5 microm long and 0.6-1.2 microm wide and had prominent longitudinally arranged microtubules extending from the vp tips to the granular layer (=ground substance). the parasitophorous vacuolar membrane with i ... | 2001 | 11534668 |
| variation in clinical and parasitological traits in pietrain and meishan pigs infected with sarcocystis miescheriana. | future prophylaxis needs new concepts, including natural disease resistance of hosts against infectious agents. genomic approaches to detect and improve disease resistance in farm animals and the molecular mechanisms involved in host-parasite interactions depend to a high degree on the trait differences between founder breeds, i.e. on the animal model. the present study evaluates differences in susceptibility/resistance against sarcocystis miescheriana in the european pietrain (pi) and the chine ... | 2002 | 12031814 |
| toxoplasma gondii: comparison of a rhoptry-elisa with ifat and mat for antibody detection in sera of experimentally infected pigs. | indirect elisa and ifat have been reported to be more sensitive and specific than agglutination tests. however, mat is cheaper, easier than the others and does not need special equipment. the purpose of this study was to compare an enzyme linked immunosorbent assay using crude rhoptries of toxoplasma gondii as coating wells (r-elisa) with indirect fluorescence antibody test (ifat) and modified agglutination test (mat) to detect anti-t. gondii antibodies in sera of experimentally infected pigs. t ... | 2006 | 16458299 |
| effects of frozen storage on the structure of sarcocysts in pig muscle and implications in taxonomic studies. | the morphology of the cyst wall of sarcocystis has unique characteristics that can be used in species identification. to find a suitable way to preserve sarcocystis cyst samples for species identification, by light microscopy and electron microscopy, we recorded the morphological changes in the cysts of sarcocystis suihominis and sarcocystis miescheriana from pig muscle, induced by storage at -20 degrees c. comparisons were made between fresh cysts and those subjected to frozen storage for perio ... | 2007 | 17126836 |
| genetic resistance to sarcocystis miescheriana in pigs following experimental infection. | clinical and parasitological traits of sarcocystis miescheriana differ in pietrain and meishan pigs. for further description and characterization of the genetic basis of this variation a f(2) family based on pietrain boars and meishan sows as founders was generated. one hundred and thirty-nine f(2) pigs were challenged orally at an age of 100 days with 50,000 sporozysts to produce the typical clinical picture of a moderate dose sarcocystis infection. heritabilities were estimated for clinical an ... | 2007 | 17129674 |
| mapping of quantitative trait loci affecting resistance/susceptibility to sarcocystis miescheriana in swine. | the outcome of infectious diseases in vertebrates is under genetic control at least to some extent. in swine, e.g., marked differences in resistance/susceptibility to sarcocystis miescheriana have been shown between chinese meishan and european pietrain pigs, and these differences are associated with high heritabilities. a first step toward the identification of genes and polymorphisms causal for these differences may be the mapping of quantitative trait loci (qtls). considering clinical, immuno ... | 2007 | 17336038 |
| quantitative trait loci for red blood cell traits in swine. | haematological traits are essential diagnostic parameters in veterinary practice but knowledge on the genetic architecture controlling variability of erythroid traits is sparse, especially in swine. to identify qtl for erythroid traits in the pig, haematocrit (hct), haemoglobin (hb), erythrocyte counts (rbc) and mean corpuscular haemoglobin content (mchc) were measured in 139 f(2) pigs from a meishan/pietrain family, before and after challenge with the protozoan pathogen sarcocystis miescheriana ... | 2007 | 17627803 |
| quantitative trait loci for white blood cell numbers in swine. | differential white blood cell counts are essential diagnostic parameters in veterinary practice but knowledge on the genetic architecture controlling variability of leucocyte numbers and relationships is sparse, especially in swine. total leucocyte numbers (leu) and the differential leucocyte counts, i.e. the fractions of lymphocytes (lym), polymorphonuclear leucocytes [neutrophils (neu), eosinophils (eos) and basophils (bas)] and monocytes (mon) were measured in 139 f(2) pigs from a meishan/pie ... | 2008 | 18307579 |
| mapping of quantitative trait loci for clinical-chemical traits in swine. | clinical-chemical traits are diagnostic parameters essential for characterization of health and disease in veterinary practice. the traits show significant variability and are under genetic control, but little is known about the fundamental genetic architecture of this variability, especially in swine. we have identified qtl for alkaline phosphatase (alp), lactate (lac), bilirubin (bil), creatinine (cre) and ionized sodium (na(+)), potassium (k(+)) and calcium (ca(++)) from the serum of 139 f(2) ... | 2009 | 19016680 |
| heritabilities and quantitative trait loci for blood gases and blood ph in swine. | maintaining ph and blood gases in a narrow range is essential to sustain normal biochemical reactions. decreased oxygenation, poor tissue perfusion, disturbance to co(2) expiration, and shortage of hco(3)(-) can lead to metabolic acidosis. this is a common situation in swine, and originates from a broad range of medical conditions. ph and blood gases appear to be under genetic control, and populations with physiological traits closer to the pathological thresholds may be more susceptible to deve ... | 2009 | 19076735 |
| mapping of quantitative trait loci affecting behaviour in swine. | behavioural indices in vertebrates are under genetic control at least to some extent. in spite of significant behavioural problems in farm animals, information on the genetic background of behaviour is sparse. the aim of this study was to map qtl for behavioural indices in swine under healthy conditions and after infection with sarcocystis miescheriana, as behaviour can be significantly influenced by disease. this well-described parasite model subsequently leads to acute (day 14 p.i.), subclinic ... | 2009 | 19291137 |
| first molecular identification of sarcocystis miescheriana (protozoa, apicomplexa) from wild boar (sus scrofa) in iran. | sarcocystis isolate obtained from the thigh muscle of a wild boar (sus scrofa), captured from gilan province, northern iran, was subjected to molecular analysis. genomic dna was obtained using a dna extraction tissue kit and polymerase chain reaction (pcr) for amplification of the 18s ribosomal dna region yielded an 842 bp dna band on agarose gel. analysis of dna sequencing by blast confirmed the isolate as sarcocystis miescheriana and the sequence was deposited in genbank by accession no. gu395 ... | 2010 | 21095184 |
| first report of naturally acquired clinical sarcocystosis in a pig breeding stock. | a two-year-old large white boar from a pig breeding stock in an indoor farm in switzerland presented anorexia, reduced general condition and fever. despite antibiotic and anti-inflammatory treatment, the boar developed severe dyspnoea and cyanosis, and died after 4 days. at necropsy, no gross lesions were observed. histopathologically, multifocal degeneration and necrosis of myocardial fibers with interstitial edema, severe multifocal non-suppurative myocarditis and hepatitis, and non-suppurativ ... | 2010 | 21145659 |
| an intronic polymorphism in the porcine irf7 gene is associated with better health and immunity of the host during sarcocystis infection, and affects interferon signalling. | interferon regulatory factor 7 (irf7), as a key regulator of type i interferon response, plays an important role during innate response against viral infection. although well conserved across species, the structure of irf7 and its function during parasite infection are not well documented in farm animals, such as the pig. to bridge this gap, we have determined the porcine irf7 gene structure and identified two intronic single nucleotide polymorphisms (snps), snp g.748g>c and snp g.761a>g, in com ... | 2011 | 21749421 |
| detection of zoonotic protozoa toxoplasma gondii and sarcocystis suihominis in wild boars from spain. | food safety regulations require the control of the presence of protozoa in meats destined for human consumption. wild boar (sus scrofa) meat may constitute a source of zoonoses. a 23.8% (688/2881) seroprevalence of anti-toxoplasma gondii antibodies and 72.2% (662/910) sarcocystis sarcocysts prevalence were detected among wild boars hunted in southwestern areas of spain. identity of sarcocystis spp. was performed by rflp-pcr and sequencing, detecting s. miescheriana (7/8) and the zoonotic s. suih ... | 2016 | 26604045 |
| unraveling sarcocystis miescheriana and sarcocystis suihominis infections in wild boar. | sarcocystis species are worldwide spread cyst-forming protozoa that can infect wild boar but little is known about the prevalence of these parasites. in this study we assessed the prevalence of sarcocystis spp. infections in wild boars from northeastern portugal, for which novel pcr testing assays targeting sarcocystis genus, s. miescheriana and s. suihominis were implemented, and risk factors potentially associated with these infections were evaluated. samples from muscle tissue, namely diaphra ... | 2015 | 26319199 |
| in the united states, negligible rates of zoonotic sarcocystosis occur in feral swine that, by contrast, frequently harbour infections with sarcocystis miescheriana, a related parasite contracted from canids. | transmission of pathogens between domestic and wild life animals plays an important role in epidemiology. feral pig populations are increasing and expanding in the usa, and may constitute a risk to non-biosecure domestic pig facilities by serving as reservoirs for pathogens. we surveyed, for sarcocystis infection, the myocardium of 1006 feral pigs (sus scrofa) trapped or hunted in 29 states during the comprehensive feral swine disease surveillance program of the usda's animal and plant health in ... | 2015 | 25363485 |
| morphological and molecular characterization of sarcocystis miescheriana from pigs in the central region of china. | sarcocystosis is an important food-borne parasitosis in humans and various animals. sarcocystis miescheriana and sarcocystis suihominis are pathogenic to pigs; s. suihominis is also distinctly pathogenic to humans. intermediate and final hosts can harbor more than one sarcocystis species, so the exact identification for sarcocystis infection in various hosts is essential to control sarcocystosis in humans and important economic animals including pigs. in this study, four isolates of sarcocysts f ... | 2013 | 23224613 |