| taxonomic characterization of ochrobactrum sp. isolates from soil samples and wheat roots, and description of ochrobactrum tritici sp. nov. and ochrobactrum grignonense sp. nov. | a large collection of bacterial strains, immunotrapped from soil and from the wheat rhizoplane, was subjected to polyphasic taxonomy by examining various pheno- and genotypic parameters. strains were grouped on (inter) repetitive extragenic palindromic dna (rep) pcr profiles at the intraspecies level. pheno- and genotypic characters were assessed for representatives from 13 different rep groups. strains of nine rep groups constituting two physiological biolog clusters fell in the coherent dna-dn ... | 2000 | 11155998 |
| towards a standardized format for the description of a novel species (of an established genus): ochrobactrum gallinifaecis sp. nov. | a format for the description of single novel species is proposed, which should facilitate the reviewing process by assisting the provision of data in a standardized form. the abstract must be short and concise, highlighting phylogenetic position, morphology and chemotaxonomy for genus affiliation, the genotypic and phenotypic basis for species differentiation, and the name and deposition numbers from two public culture collections in different countries for the type strain: a gram-negative, rod- ... | 2003 | 12807218 |
| ochrobactrum tritici strain 5bvl1 - characterization of a cr(vi)-resistant and cr(vi)-reducing strain. | bacterial strain 5bvl1, isolated from a chromium-contaminated wastewater treatment plant and identified as ochrobactrum tritici, was resistant to a broad range of antibiotics, to cr(vi), ni(ii), co(ii), cd(ii), and zn(ii), and was able to grow in the presence of 5% nacl and within the ph range 4-10. characterization showed that strain 5bvl1 could be considered a halotolerant and alkalitolerant microorganism resistant to high concentrations of cr(vi). this strain was able to grow aerobically in u ... | 2004 | 15644923 |
| pulsed-field gel electrophoresis to study the diversity of whole-genome organization in the genus ochrobactrum. | the alpha-proteobacterial genus ochrobactrum groups together organisms that display varied life-styles, such as free-living bacteria, members of rhizosphere and soil, nitrogen-fixing bacteria in plant nodules, xenobiotic-degrading bacteria, colonizers of nematodes and insects, and opportunistic human pathogens. the genomes of nine strains of ochrobactrum anthropi and eight strains of ochrobactrum intermedium were analyzed by pulsed-field gel electrophoresis of the whole genome and of i-ceui dige ... | 2005 | 15995979 |
| genetic and phenotypic microdiversity of ochrobactrum spp. | the diversity of ochrobactrum anthropi, ochrobactrum intermedium, ochrobactrum tritici and ochrobactrum grignonense in agricultural soil and on the wheat rhizoplane was investigated. o. anthropi was isolated both from soil and from the rhizoplane, o. intermedium and grignonense only from bulk soil, and o. tritici only from the wheat rhizoplane. on the genetic level, the immunotrapped isolates and a number of strains from culture collection mainly of clinical origin were compared with rep-pcr pro ... | 2006 | 16629756 |
| ochrobactrum cytisi sp. nov., isolated from nodules of cytisus scoparius in spain. | two strains named esc1(t) and esc5 were isolated from nodules of cytisus scoparius growing in a spanish soil. phylogenetic analysis of the 16s rrna gene showed that these strains belong to the genus ochrobactrum, their closest relatives being ochrobactrum anthropi and ochrobactrum lupini, with 100 and 99.9 % similarity to the respective type strains. despite this high similarity, the results of dna-dna hybridization, phenotypic tests and fatty acid analyses showed that these strains represent a ... | 2007 | 17392207 |
| sequencing and expression of two arsenic resistance operons with different functions in the highly arsenic-resistant strain ochrobactrum tritici scii24t. | arsenic (as) is a natural metalloid, widely used in anthropogenic activities, that can exist in different oxidation states. throughout the world, there are several environments contaminated with high amounts of arsenic where many organisms can survive. the most stable arsenical species are arsenate and arsenite that can be subject to chemically and microbiologically oxidation, reduction and methylation reactions. organisms surviving in arsenic contaminated environments can have a diversity of me ... | 2008 | 18554386 |
| the chromate-inducible chrbacf operon from the transposable element tnotchr confers resistance to chromium(vi) and superoxide. | large-scale industrial use of chromium(vi) has resulted in widespread contamination with carcinogenic chromium(vi). the abilities of microorganisms to survive in these environments and to detoxify chromate require the presence of specific resistance systems. here we report identification of the transposon-located (tnotchr) chromate resistance genes from the highly tolerant strain ochrobactrum tritici 5bvl1 surviving chromate concentrations of >50 mm. the 7,189-bp-long tnotchr of the mixed tn21/t ... | 2008 | 18776016 |
| identification of an aox system that requires cytochrome c in the highly arsenic-resistant bacterium ochrobactrum tritici scii24. | microbial biotransformations have a major impact on environments contaminated with toxic elements, including arsenic, resulting in an increasing interest in strategies responsible for how bacteria cope with arsenic. in the present work, we investigated the metabolism of this metalloid in the bacterium ochrobactrum tritici scii24. this heterotrophic organism contains two different ars operons and is able to oxidize arsenite to arsenate. the presence of arsenite oxidase genes in this organism was ... | 2009 | 19525272 |
| different physiological responses to chromate and dichromate in the chromium resistant and reducing strain ochrobactrum tritici 5bvl1. | studies of cr(vi) toxicity are generally performed using chromate salts in solution, both when studying the effects on prokaryotes and eukaryotes. some studies on human carcinogenesis and toxicology on bacteria were done using dichromate, but comparison with chromate was never reported before, and dichromate existence was never taken into consideration and usually overlooked. this paper studied comparatively the effect of dichromate and chromate on the physiology of ochrobactrum tritici strain 5 ... | 2010 | 20390438 |
| chromium resistance strategies and toxicity: what makes ochrobactrum tritici 5bvl1 a strain highly resistant. | large-scale industrial use of chromium (cr) resulted in widespread environmental contamination with hexavalent chromium (cr(vi)). the ability of microorganisms to survive in these environments and detoxify chromate requires the presence of specific resistance systems. several cr(vi) resistant species, belonging to a variety of genera, have been isolated in recent years. ochrobactrum tritici strain 5bvl1 is a model for a highly cr(vi)-resistant and reducing microorganism, with different strategie ... | 2011 | 21472416 |
| metal-induced phosphate extracellular nanoparticulate formation in ochrobactrum tritici 5bvl1. | hexavalent chromium (cr(vi)) is a toxic environmental contaminant which detoxification consists in reduction to cr(iii). in this work, the cr(vi)-resistant and reducing ochrobactrum tritici 5bvl1 produced phosphate nanoparticles upon exposure to cr(vi) and fe(iii), effectively removing chromium from solution. under cr(vi) stress, higher siderophore production by strain 5bvl1 was observed. cr(vi) toxicity was decreased in presence of fe(iii), increasing the growth and cr(vi)-reduction rates in ce ... | 2011 | 22018866 |
| two superoxide dismutases from tnotchr are involved in detoxification of reactive oxygen species induced by chromate. | superoxide dismutases (sod) have been reported as the most relevant bacterial enzymes involved in cells protection from reactive oxygen species (ros). these toxic species are often the product of heavy metal stress. | 2016 | 26944876 |
| first case report of human infection with ochrobactrum tritici causing bacteremia and cholecystitis. | | 2016 | 26915622 |
| identification and characterization of the transcriptional regulator chrb in the chromate resistance determinant of ochrobactrum tritici 5bvl1. | ochrobactrum tritici 5bvl1 is able to resist to high concentrations of chromate through the expression of an inducible chromate-resistant determinant, found in a mobile element (tnotchr), which carries the genes, chrb, chra, chrc and chrf. the regulation of chr operon present in tnotchr, which is controlled by a transcriptional regulator, chrb, was characterized in the current work. fusions of chr promoter, or chr promoter and chrb gene, upstream of a gfp reporter gene, identified the most proba ... | 2013 | 24223748 |
| highly sensitive, highly specific whole-cell bioreporters for the detection of chromate in environmental samples. | microbial bioreporters offer excellent potentialities for the detection of the bioavailable portion of pollutants in contaminated environments, which currently cannot be easily measured. this paper describes the construction and evaluation of two microbial bioreporters designed to detect the bioavailable chromate in contaminated water samples. the developed bioreporters are based on the expression of gfp under the control of the chr promoter and the chrb regulator gene of tnotchr determinant fro ... | 2013 | 23326558 |
| immobilization of ochrobactrum tritici as5 on ptfe thin films for arsenite biofiltration. | ochrobactrum tritici scii24t bacteria is an environmental strain with high capacity to resist to arsenic (as) toxicity, which makes it able to grow in the presence of as(iii). the inactivation of the two functional arsenite efflux pumps, arsb and acr3_1, resulted in the mutant o. tritici as5 exhibiting a high accumulation of arsenite. this work describes a method for the immobilization of the mutant cells o. tritici as5, on a commercial polymeric net after sputtered modified by the deposition of ... | 2016 | 26735734 |
| hyper accumulation of arsenic in mutants of ochrobactrum tritici silenced for arsenite efflux pumps. | ochrobactrum tritici scii24t is a highly as-resistant bacterium, with two previously described arsenic resistance operons, ars1 and ars2. among a large number of genes, these operons contain the arsb and acr3 genes that encode the arsenite efflux pumps responsible for arsenic resistance. exploring the genome of o. tritici scii24t, an additional putative operon (ars3) was identified and revealed the presence of the acr3_2 gene that encodes for an arsenite efflux protein but which came to prove to ... | 2015 | 26132104 |