| taxonomic characterization of ochrobactrum sp. isolates from soil samples and wheat roots, and description of ochrobactrum tritici sp. nov. and ochrobactrum grignonense sp. nov. | a large collection of bacterial strains, immunotrapped from soil and from the wheat rhizoplane, was subjected to polyphasic taxonomy by examining various pheno- and genotypic parameters. strains were grouped on (inter) repetitive extragenic palindromic dna (rep) pcr profiles at the intraspecies level. pheno- and genotypic characters were assessed for representatives from 13 different rep groups. strains of nine rep groups constituting two physiological biolog clusters fell in the coherent dna-dn ... | 2000 | 11155998 |
| [species identification and molecular epidemiology of bacteria belonging to ochrobactrum genus]. | two species of medical interest belong to the genus ochrobactrum, ochrobactrum anthropi and ochrobactrum intermedium. they are members of the microbiota of soil and an increasing number of works report the isolation of o. anthropi from clinical specimen, especially from immunocompromised patients and nosocomial infection. involving of each species in human infection is poorly estimated due to unclear differential phenotypic characters. we performed 16s rdna sequencing for identification of 20 cl ... | 2003 | 12628286 |
| comparative study of cr(vi) uptake and reduction in industrial effluent by ochrobactrum intermedium and brevibacterium sp. | two chromium-resistant bacterial strains, crt-1 and crt-13, tolerant up to 40 mg k2cro4 ml(-1) on nutrient agar, 25 mg ml(-1) in nutrient broth, and up to 10 mg ml(-1) in acetate-minimal media, were identified as ochrobactrum intermedium and brevibacterium sp., respectively, on the basis of 16s rrna gene sequencing. uptake of chromate was greater in living cells than in heat-killed on dried cells. crt-1 reduced 82%, 28% and 16% of cr(vi) at 100, 500, and 1000 microg ml(-1) after 24 h while crt-1 ... | 2004 | 15604809 |
| genotyping of ochrobactrum spp. by aflp analysis. | aflp was used to analyze the genetic diversity among ochrobactrum strains. aflp patterns showed a great genomic variability that separated the samples into three distinct clusters. ochrobactrum intermedium was found to be closely related to brucella abortus s99. | 2005 | 15774899 |
| evaluation of ochrobactrum intermedium bacteremia in a patient with bladder cancer. | it is generally believed that ochrobactrum anthropi is the only pathogenic species in the genus ochrobactrum. we report a case of presumptive ochrobactrum intermedium bacteremia in a patient with bladder cancer. identification of a rare species using rapid commercial panel could lead to an erroneous result as this clinical isolate was initially reported to be shewanella putrefaciens. the patient was successfully treated with imipenem and ciprofloxacin. | 2005 | 16168615 |
| pelvic abscess due to ochrobactrum intermedium [corrected] in an immunocompetent host: case report and review of the literature. | ochrobactrum intermedium [corrected] infection is rare in humans and is generally associated with immunocompromised hosts with indwelling foreign bodies. we report a case of pelvic abscess with o. intermedium [corrected] after a routine appendectomy in an immunocompetent patient and review the literature on o. intermedium [corrected] infection in patients with normal immune function. | 2006 | 16517927 |
| atypical 16s rrna gene copies in ochrobactrum intermedium strains reveal a large genomic rearrangement by recombination between rrn copies. | ochrobactrum intermedium is an opportunistic human pathogen belonging to the alpha 2 subgroup of proteobacteria. the 16s rdna sequences of nine o. intermedium isolates from a collection of clinical and environmental isolates exhibited a 46-bp insertion at position 187, which was present in only one sequence among the 82 complete or partial 16s rdna sequences of ochrobactrum spp. available in data banks. reverse transcription-pcr experiments showed that the 46-bp insertion remained in the 16s rrn ... | 2003 | 12700269 |
| occurrence of natural dixenic associations between the symbiont photorhabdus luminescens and bacteria related to ochrobactrum spp. in tropical entomopathogenic heterorhabditis spp. (nematoda, rhabditida). | bacteria naturally associated with the symbiont photorhabdus luminescens subsp. akhurstii were isolated from the entomopathogenic nematode heterorhabditis indica. bacterial isolates distinct from p. luminescens subsp. akhurstii were obtained from 33% of the samples. fourteen bacterial isolates, from nematodes collected from three different caribbean islands, were characterized by conventional phenotypic tests, restriction fragment length polymorphism and sequence analyses of pcr-amplified 16s rr ... | 2000 | 10746775 |
| ochrobactrum intermedium infection after liver transplantation. | a case of bacteremia due to ochrobactrum intermedium, with concomitant liver abscesses, in an orthotopic liver transplant recipient is presented. identical microorganisms were isolated from fecal specimens and from an aspirate of a liver abscess that was indicative of invasion of the graft by gastrointestinal spread. 16s dna sequence analysis of the blood isolate revealed the recovery of the recently proposed new species o. intermedium, closely related to ochrobactrum anthropi and brucella spp. | 1999 | 9854103 |
| evaluation of the relatedness of brucella spp. and ochrobactrum anthropi and description of ochrobactrum intermedium sp. nov., a new species with a closer relationship to brucella spp. | the relatedness of brucella spp. and ochrobactrum anthropi was studied by protein profiling, western blot, immunoelectrophoresis and 16s rrna analysis. whole-cell and soluble proteins of brucellae and o. anthropi showed serological cross-reactivities quantitatively and qualitatively more intense than those existing with similar extracts of agrobacterium spp. numerical analysis of western blot profiles of whole-cell extracts showed that o. anthropi lmg 3301 was closer to brucella spp. than to o. ... | 1998 | 9734029 |
| ochrobactrum pecoris sp. nov., isolated from farm animals. | two gram-negative, rod-shaped, non-spore-forming strains, 08rb2639(t) and 08rb2781-1 isolated from sheep (ovis aries) and boar (sus scrofa domestica), respectively, were studied for their taxonomic position. by 16s rrna gene sequencing, these strains revealed identical sequences and were shown to belong to the alphaproteobacteria with 97.8 % sequence similarity to ochrobactrum rhizosphaerae pr17(t), ochrobactrum pituitosum ccug 50899(t) , ochrobactrum tritici scii24(t) and ochrobactrum haematoph ... | 2010 | 20952542 |
| ochrobactrum ciceri sp. nov., isolated from nodules of cicer arietinum. | a gram-staining-negative, aerobic, rod-shaped, non-spore-forming bacterial strain, ca-34(t), was isolated from nodules of chickpea (cicer arietinum) in pakistan and studied for its taxonomic affiliation. the almost full-length 16s rrna gene sequence showed highest similarities to those of strains of the genus ochrobactrum. based on results of maldi-tof ms and 16s rrna gene sequence similarity (98.6 %), strain ca-34(t) and ochrobactrum intermedium lmg 3301(t) are phylogenetic neighbours; the two ... | 2010 | 19684324 |
| isolation of urease-positive ochrobactrum intermedium in the stomach of a non-ulcer dyspeptic patient from north india. | ochrobactrum intermedium is an opportunistic human pathogen found in immunocompromised individuals. we report the case of a north indian patient with non-ulcer dyspepsia whose gastric biopsy revealed the presence of o. intermedium, along with helicobacter pylori. further description of o. intermedium was performed with 16s rrna (1500 nucleotides) and reca (1065 nucleotides) gene sequencing, and the identity and phylogenetic affiliation of the isolate was confirmed by 100% nucleotide similarity w ... | 2008 | 18473108 |
| genetic diversity and phylogenetic relationships of bacteria belonging to the ochrobactrum-brucella group by reca and 16s rrna gene-based comparative sequence analysis. | the genetic diversity and phylogenetic interrelationships among 106 ochrobactrum strains (o. anthropi: 72, o. intermedium: 22, o. tritici: 5, o. oryzae: 2, o. grignonense: 2, o. gallinifaecis: 1, o. lupini: 2), the type strains of the eight brucella species and other closely related taxa were studied by reca and rrs gene (16s rrna) comparative sequence analysis. both markers correctly delineated the various ochrobactrum species; however, resolution at the subspecies level was considerably higher ... | 2008 | 18222618 |
| specific detection and differentiation of ochrobactrum anthropi, ochrobactrum intermedium and brucella spp. by a multi-primer pcr that targets the reca gene. | ochrobactrum anthropi, ochrobactrum intermedium and brucella spp. are phenotypically and genetically closely related pathogens that may cause disease with similar clinical presentation. consequently, difficulties in their identification and differentiation have been reported. in this study, a sensitive reca gene-based multi-primer single-target pcr (mp-st-pcr) was developed that allowed the specific detection and differentiation of these clinically relevant pathogens. the specificity of the assa ... | 2008 | 18065669 |
| brucellosis of the common vole (microtus arvalis). | a systemic disease occurred in a wild population of the common vole microtus arvalis in south moravia (czech republic) during the years 1999-2003. acute infections were characterized by edema of extremities, occasionally with colliquating abscesses, arthritis, lymphadenitis, perforations of the skin resulting from colliquated abscesses, orchitis, and peritoneal granulomas. from the clinical samples, small gram-negative coccobacilli were isolated and identified as ochrobactrum intermedium by api ... | 2007 | 18021023 |
| [ochrobactrum intermedium anki, a nitrogen-fixing bacterium able to decolorize azobenzene]. | morphological and biochemical properties of the nitrogen-fixing strain ochrobactrum intermedium anki, intensely growing on media with azo compounds, and its resistance to various common xenobiotics were investigated. the kinetics of azobenzene conversion by o. intermedium anki was studied. under cometabolism conditions, up to 40 mg of azobenzene per liter of medium were decolorized within one week. it was shown that the strain possessed molybdenum-dependent nitrogenase activity, and its nitrogen ... | 2007 | 17929573 |
| ochrobactrum pseudintermedium sp. nov., a novel member of the family brucellaceae, isolated from human clinical samples. | three novel gram-negative, non-fermenting aerobic bacilli were isolated from human clinical samples. they shared more than 99.8 % of the 16s rrna gene nucleotide positions. the strains were related to ochrobactrum intermedium with about 97.48 % 16s rrna gene sequence similarity. in 16s rrna gene-, dnak- and rpob-based phylogenies, the strains were grouped in a lineage that was distinct from other ochrobactrum species in the family brucellaceae. fatty acid composition, polar lipids, quinone syste ... | 2007 | 17473249 |
| biodegradation of nicotine from tobacco waste extract by ochrobactrum intermedium dn2. | ochrobactrum intermedium dn2 was used to degrade nicotine in tobacco waste extracts. the optimal temperature and ph of nicotine degradation by strain dn2 was 30-37 degrees c and 7.0, respectively. under these optimal conditions, the average degradation rate of nicotine in a 30l fed-batch culture was 140.5 mg 1(-1) h(-1). the results of this study indicate that strain dn2 may be useful for reducing the nicotine content of reconstituted tobacco. | 2007 | 17333093 |
| growth stimulatory effect of ochrobactrum intermedium and bacillus cereus on vigna radiata plants. | this study assessed the plant growth-promoting ability of the bacterial strains ochrobactrum intermedium (isolate crt-1) and bacillus cereus (isolate s-6). | 2006 | 16965380 |
| optimization of a medium for enhancing nicotine biodegradation by ochrobactrum intermedium dn2. | to optimize a medium for nicotine degradation by ochrobactrum intermedium dn2 in presence of yeast extract, glucose and tween 80 using response surface methodology (rsm). | 2006 | 16907819 |
| microorganisms and heavy metals associated with atmospheric deposition in a congested urban environment of a developing country: sri lanka. | the presence of bacteria and heavy metals in atmospheric deposition were investigated in kandy, sri lanka, which is a typical city in the developing world with significant traffic congestion. atmospheric deposition samples were analyzed for al, cr, mn, fe, ni, cu, zn, cd and pb which are heavy metals common to urban environments. al and fe were found in high concentrations due to the presence of natural sources, but may also be re-suspended by vehicular traffic. relatively high concentrations of ... | 2017 | 28185730 |
| genetic and phenotypic microdiversity of ochrobactrum spp. | the diversity of ochrobactrum anthropi, ochrobactrum intermedium, ochrobactrum tritici and ochrobactrum grignonense in agricultural soil and on the wheat rhizoplane was investigated. o. anthropi was isolated both from soil and from the rhizoplane, o. intermedium and grignonense only from bulk soil, and o. tritici only from the wheat rhizoplane. on the genetic level, the immunotrapped isolates and a number of strains from culture collection mainly of clinical origin were compared with rep-pcr pro ... | 2006 | 16629756 |
| measurement of biochemical oxygen demand from different wastewater samples using a mediator-less microbial fuel cell biosensor. | microbial fuel cells (mfcs) have attracted considerable attention as potential biosensors. a mfc biosensor for rapid measurement of biochemical oxygen demand (bod) has been recently studied. however, a standardized bacterial mixture inoculated in the mfc biosensor for bod measurement is unavailable. thus, the commercial application of a mfc biosensor is limited. in this study, a mediator-less mfc biosensor inoculated with known mixed cultures to quickly determine bod concentration was tested. op ... | 2014 | 25145173 |
| reduction of toxic hexavalent chromium by ochrobactrum intermedium strain sdcr-5 stimulated by heavy metals. | a cr(vi) resistant bacterial strain sdcr-5, identified as ochrobactrum intermedium on the basis of 16s rrna gene sequencing, was tolerant to high concentrations of cr(vi) up to 15 mg ml(-1) in acetate minimal medium. o. intermedium sdcr-5 reduced cr(vi) under a wide range of concentrations from 100 to 1500 microg ml(-1) and reduction was optimum at 37 degrees c and ph 7. it reduced 200 and 721 microg ml(-1) cr(vi) within 72 and 96 h, respectively. the rate of cr(vi) reduction increased with conc ... | 2007 | 16488604 |
| beneficial role of hydrophytes in removing cr(vi) from wastewater in association with chromate-reducing bacterial strains ochrobactrum intermedium and brevibacterium. | this study deals with the use of three chromium-resistant bacterial strains (ochrobactrum intermedium crt-1, brevibacterium crt-13, and crm-1) in conjunction with eichornia crassipes for the removal of toxic chromium from wastewater. bacterial strains resulted in reduced uptake of chromate into inoculated plants as compared to noninoculated control plants. in the presence of different heavy metals, chromium uptake into the plants was 28.7 and 7.15% less at an initial k2cro4 concentration of 100 ... | 2005 | 16463540 |
| comparative sequence analysis of the internal transcribed spacer 1 of ochrobactrum species. | the internal 16s/23s rdna (rrs/rrl) internal spacer region 1 (its1) of 54 ochrobactrum strains and close relatives was analysed. separation of its1 containing pcr products by gel-electrophoresis, dgge, cloning and sequencing revealed its1 length and sequence heterogeneity. we found up to 5 different allelic its1 stretches within a single strain (ochrobactrum intermedium lmg 3301t), and 2-3 different its1 alleles in o. tritici. within its1, its1c, being part of the conserved double-stranded rrn p ... | 2006 | 16352412 |
| molecular and phenotypic features for identification of the opportunistic pathogens ochrobactrum spp. | among the six species characterized within the genus ochrobactrum, ochrobactrum anthropi and ochrobactrum intermedium are currently reported as opportunistic pathogens in humans. since the species identification is mainly based on 16s rdna analysis, the aim of this study was to search for other characteristics useful for ochrobactrum species discrimination. ribotyping, morphological and biochemical analyses, and antimicrobial susceptibility testing were performed for a panel of 35 clinical isola ... | 2005 | 16157548 |
| bacterial cr(vi) reduction concurrently improves sunflower (helianthus annuus l.) growth. | four cr(vi)-reducing bacterial strains (ochrobactrum intermedium, crt-2, crt-3 and crt-4) previously isolated from chromium-contaminated sites were inoculated on to seeds of sunflower (helianthus annuus var sf-187), which were germinated and grown along with non-inoculated controls with chromate salts (300 microg crcl3 or k2cro4 ml(-1)). severe reduction (20%) in seed germination was observed in cr(vi) stress. plant height decreased (36%) with cr(vi) when compared with chromium-free control, whi ... | 2005 | 16091890 |
| pulsed-field gel electrophoresis to study the diversity of whole-genome organization in the genus ochrobactrum. | the alpha-proteobacterial genus ochrobactrum groups together organisms that display varied life-styles, such as free-living bacteria, members of rhizosphere and soil, nitrogen-fixing bacteria in plant nodules, xenobiotic-degrading bacteria, colonizers of nematodes and insects, and opportunistic human pathogens. the genomes of nine strains of ochrobactrum anthropi and eight strains of ochrobactrum intermedium were analyzed by pulsed-field gel electrophoresis of the whole genome and of i-ceui dige ... | 2005 | 15995979 |
| [isolation and identification of a strain dn2 degrading nicotine]. | a bacterial strain which was able to utilize nicotine as its sole carbon source was isolated from soil in which tobacco had grown at sanming region in fujian province and named as dn2. upon chemotaxonomic characterization and phylogenetic inference based 16s rdna analysis, the strain dn2 was identified as a-proteobacteria, ochrobactrum intermedium. for dn2 degrading nicotine, the optimal ph and temperature is 6.5, 30 degrees c respectively. it can tolerate high-concentration of nicotine up to 40 ... | 2005 | 15989256 |
| bacterial reduction of toxic cr (vi) into cr (iii). | two chromium-resistant bacterial strains crt-1 and crt-13, which can tolerate k2cro4 up to 40 mg x ml(-1) on nutrient agar, 25 mg x ml(-1) k2 cro4 in nutrient broth, and up to 10 mg x ml(-1) in acetate-minimal media, were used in this study. on the basis of 16s rrna, strain crt-1 was identified as ochrobactrum intermedium and crt-13 as brevibacterium sp.. uptake of chromate was greater in living cells than in heat-killed cells. ochrobactrum intermedium crt-1 reduced 73% and 41% of cr(v) while br ... | 2004 | 15974008 |
| chromium-resistant bacteria and cyanobacteria: impact on cr(vi) reduction potential and plant growth. | two chromium-resistant bacterial strains, bacillus cereus s-6 and ochrobactrum intermedium crt-1, and two cyanobacterial strains, oscillatoria sp. and synechocystis sp., were used in this study. at initial chromate concentrations of 300 and 600 microg k2cro4 ml(-1), and an inoculum size of 9.6 x 10(7) cells ml(-1), b. cereus s-6 completely reduced cr(vi), while o. intermedium crt-1 reduced cr(vi) by 98% and 70%, respectively after 96 h. at 100 microg k2cro4 ml(-1), synechocystis sp. mk(s) and os ... | 2005 | 15926088 |
| application of a continuously stirred tank bioreactor (cstr) for bioremediation of hydrocarbon-rich industrial wastewater effluents. | a continuously stirred tank bioreactor (cstr) was used to optimize feasible and reliable bioprocess system in order to treat hydrocarbon-rich industrial wastewaters. a successful bioremediation was developed by an efficient acclimatized microbial consortium. after an experimental period of 225 days, the process was shown to be highly efficient in decontaminating the wastewater. the performance of the bioaugmented reactor was demonstrated by the reduction of cod rates up to 95%. the residual tota ... | 2011 | 21419572 |
| a treatment plant receiving waste water from multiple bulk drug manufacturers is a reservoir for highly multi-drug resistant integron-bearing bacteria. | the arenas and detailed mechanisms for transfer of antibiotic resistance genes between environmental bacteria and pathogens are largely unclear. selection pressures from antibiotics in situations where environmental bacteria and human pathogens meet are expected to increase the risks for such gene transfer events. we hypothesize that waste-water treatment plants (wwtps) serving antibiotic manufacturing industries may provide such spawning grounds, given the high bacterial densities present there ... | 2013 | 24204801 |
| culturable heavy metal-resistant and plant growth promoting bacteria in v-ti magnetite mine tailing soil from panzhihua, china. | to provide a basis for using indigenous bacteria for bioremediation of heavy metal contaminated soil, the heavy metal resistance and plant growth-promoting activity of 136 isolates from v-ti magnetite mine tailing soil were systematically analyzed. among the 13 identified bacterial genera, the most abundant genus was bacillus (79 isolates) out of which 32 represented b. subtilis and 14 b. pumilus, followed by rhizobium sp. (29 isolates) and ochrobactrum intermedium (13 isolates). altogether 93 i ... | 2014 | 25188470 |
| niches, population structure and genome reduction in ochrobactrum intermedium: clues to technology-driven emergence of pathogens. | ochrobactrum intermedium is considered as an emerging human environmental opportunistic pathogen with mild virulence. the distribution of isolates and sequences described in literature and databases showed frequent association with human beings and polluted environments. as population structures are related to bacterial lifestyles, we investigated by multi-locus approach the genetic structure of a population of 65 isolates representative of the known natural distribution of o. intermedium. the p ... | 2014 | 24465379 |
| valvulopathy consistent with endocarditis in an argentine boa (boa constrictor occidentalis). | an argentine boa (boa constrictor occidentalis) of 5 yr 7 mo of age was presented for respiratory problems and regurgitation. radiographs revealed evidence of cardiomegaly and pneumonia. blood smear examination revealed the presence of intracytoplasmic inclusion bodies in peripheral lymphocytes, consistent with inclusion body disease. cultures of a tracheal wash sample resulted in growth of ochrobactrum intermedium and pseudomonas putida. echocardiographic examination revealed a large vegetative ... | 2015 | 25831585 |
| isolation and characterization of a crude oil degrading bacteria from formation water: comparative genomic analysis of environmental ochrobactrum intermedium isolate versus clinical strains. | in this study, we isolated an environmental clone of ochrobactrum intermedium, strain 2745-2, from the formation water of changqing oilfield in shanxi, china, which can degrade crude oil. strain 2745-2 is aerobic and rod-shaped with optimum growth at 42 °c and ph 5.5. we sequenced the genome and found a single chromosome of 4 800 175 bp, with a g+c content of 57.63%. sixty rnas and 4737 protein-coding genes were identified: many of the genes are responsible for the degradation, emulsification, a ... | 2015 | 26465134 |
| intravitreal moxifloxacin in the management of ochrobactrum intermedium endophthalmitis due to metallic intraocular foreign body. | a healthy 34-year-old man presented with ochrobactrum intermedium endophthalmitis due to a metallic intraocular foreign body. after vitrectomy, lensectomy, removal of the metallic intraocular foreign body, intravitreal vancomycin and ceftazidime, and systemic ciprofloxacin, intraocular inflammation worsened. repeat vitreous culture confirmed persistent endophthalmitis due to multidrug-resistant o. intermedium. the endophthalmitis successfully resolved after the administration of intravitreal mox ... | 2013 | 24039392 |
| isolation and characterisation of endophytic nitrogen fixing bacteria in sugarcane. | endophytic nitrogen fixing bacteria were isolated from the leaves, stems and roots of industrial variety (cv. u-thong 3; ut3), wild and chewing sugarcane plants grown for 6 weeks in nitrogen (n)-free sand. eighty nine isolates of endophytic bacteria were obtained on n-free agar. an acetylene reduction assay (ara) detected nitrogenase activity in all 89 isolates. three isolates from the chewing (c2hl2, c7hl1 and c34mr1) sugarcane and one isolate from the industrial sugarcane (ut3r1) varieties wer ... | 2015 | 26868592 |
| ochromobactrum intermedium: an emerging opportunistic pathogen-case of recurrent bacteraemia associated with infective endocarditis in a haemodialysis patient. | we describe the first clinical case report of infective endocarditis related to ochrobactrum intermedium infection. the case involved a 23-year-old man receiving dialysis via an internal jugular long-term haemodialysis catheter. he improved with a prolonged course of meropenem and minocycline. ochrobactrum spp. are recognized as rare emerging opportunistic pathogens. | 2016 | 27843545 |
| colonisation resistance in the sand fly gut: leishmania protects lutzomyia longipalpis from bacterial infection. | phlebotomine sand flies transmit the haemoflagellate leishmania, the causative agent of human leishmaniasis. the leishmania promastigotes are confined to the gut lumen and are exposed to the gut microbiota within female sand flies. here we study the colonisation resistance of yeast and bacteria in preventing the establishment of a leishmania population in sand flies and the ability of leishmania to provide colonisation resistance towards the insect bacterial pathogen serratia marcescens that is ... | 2014 | 25051919 |
| bioremediation assessment of diesel-biodiesel-contaminated soil using an alternative bioaugmentation strategy. | this study investigated the effectiveness of successive bioaugmentation, conventional bioaugmentation, and biostimulation of biodegradation of b10 in soil. in addition, the structure of the soil microbial community was assessed by polymerase chain reaction-denaturing gradient gel electrophoresis. the consortium was inoculated on the initial and the 11th day of incubation for successive bioaugmentation and only on the initial day for bioaugmentation and conventional bioaugmentation. the experimen ... | 2014 | 24091525 |
| phylogenetic profiling of bacterial community from two intimately located sites in balramgari, north-east coast of india. | microbial communities in coastal subsurface sediments play an important role in biogeochemical cycles. in this study microbial communities in tidal subsurface sediments of balramgari in the state of orissa, india were investigated using a culture independent approach. two 16s rdna cloned libraries were prepared from the closely located (100 m along the coast) subsurface sediment samples. library i sediment samples had higher organic carbon content but lower sand percentage in comparison to libra ... | 2009 | 23100766 |
| clinical and histological features of brucellin skin test responses in brucella suis biovar 2 infected pigs. | current serological tests for swine brucellosis detect antibodies to the brucella o-polysaccharide (o/ps). however, when infections by bacteria carrying cross-reacting o/ps occur, these tests suffer from false positive serological reactions (fpsr), and the skin test with brucella soluble protein extracts is the best diagnostic alternative to differentiate true brucella suis infections from fpsr in pigs. since this test has been seldom used in b. suis infected swine, the clinical and histological ... | 2015 | 25480681 |
| the synergism of temperature, ph and growth phases on heavy metal biosorption by two environmental isolates. | in real environmental applications, such as heavy metal bioremediation, microorganisms are generally not kept at their optimum growth conditions; therefore, it is imperative to investigate their heavy metal removal performance under diverse environmental conditions. the present study aims to investigate the effects of ph, temperature and growth phases on the removal of cu(2+) and cr(6+) by two environmental isolates identified as ochrobactrum intermedium lbr and cupriavidus metallidurans ch34. r ... | 2014 | 25064261 |
| isolation and characterization of pah-degrading bacteria from the eastern province, saudi arabia. | contaminated sediment samples were collected from the eastern province, saudi arabia for isolation of pyrene- and phenanthrene-degrading bacteria by enrichment method. four isolates were morphologically characterized as gram-negative rod strains and 16s rrna sequence analysis revealed the isolates as closely related to pseudomonas aeruginosa, p. citronellolis, ochrobactrum intermedium and cupriavidus taiwanensis. degradation of the polycyclic aromatic hydrocarbons (pahs) by the latter three stra ... | 2017 | 27912917 |
| a comparison of bioelectricity in microbial fuel cells with aerobic and anaerobic anodes. | microbial fuel cells (mfcs) can, besides running on wastewater, also derive energy directly from certain aquatic plants. however, few studies have focussed on electricity generation using aerobic anodes. this study presents a comparison of the mfc performances of an anaerobic-anode mfc (ana-mfc) and an aerobic-anode mfc (aa-mfc), and shows their individual conditions for optimal operation. results show that the maximum power density of 7.07 +/- 0.45 mw/m2 for the ana-mfc occurred at 500 omega, w ... | 2014 | 24600867 |
| immunological features of lps from ochrobactrum intermedium on sheep experimentally infected with fasciola hepatica. | the effects of the lipopolysaccharide (lps) from ochrobactrum intermedium in sheep with fasciolosis was reported previously, resulting in lower fecal egg counts and fluke burden. in the current study, we analyzed its immunological effects in two groups of sheep, treated (t) and controls (c). fasciolosis induces a t helper (th) type-2 response, characterized by il-4 and il-10 production; however, at the beginning of the infection, the ifn-γ production predominates (th type-1 response). although w ... | 2014 | 25151434 |
| effect of lipopolysaccharide (lps) from ochrobactrum intermedium on sheep experimentally infected with fasciola hepatica. | the effects of the lipopolysaccharide (lps) from ochrobactrum intermedium was evaluated in sheep experimentally infected with fasciola hepatica. animals were divided into four groups: two treated with the lps (t1/t2) and two controls (c1/c2). t1/c1 were slaughtered at 30 days postinfection (dpi) and t2/c2 at 85 dpi. body weight and body condition were found higher in t1 and t2 than in controls, although differences were not significant. treated sheep showed lower cumulative fecal egg count than ... | 2013 | 23739808 |
| draft genome sequence of ochrobactrum intermedium strain sa148, a plant growth-promoting desert rhizobacterium. | ochrobactrum intermedium strain sa148 is a plant growth-promoting bacterium isolated from sandy soil in the jizan area of saudi arabia. here, we report the 4.9-mb draft genome sequence of this strain, highlighting different pathways characteristic of plant growth promotion activity and environmental adaptation of sa148. | 2017 | 28254977 |
| [usefulness of maldi-tof mass spectrometry in infections by infrequent microorganisms such as ochrobactrum intermedium]. | | 2016 | 27293197 |
| isolation and characterization of lead-tolerant ochrobactrum intermedium and its role in enhancing lead accumulation by eucalyptus camaldulensis. | in this study, the potential of rhizospheric bacteria in promoting the growth and pb accumulation by the woody plant eucalyptus camaldulensis under hydroponic conditions was investigated for the first time. three pb-tolerant bacteria were isolated from the rhizosphere of e. camaldulensis grown in pb-contaminated soils in the bo ngam pb mine, thailand. based on analysis of partial 16s rrna gene sequence, the three isolates were identified as microbacterium paraoxydans bn-2, ochrobactrum intermedi ... | 2011 | 21764101 |
| reduction of hexavalent chromium by ochrobactrum intermedium bcr400 isolated from a chromium-contaminated soil. | hexavalent chromium-resistant ochrobactrum intermedium bcr400 was isolated from chromium contaminated soil collected from vadodara, gujarat. it reduced 100 mg cr(vi)/l completely in 52 h with initial cr(vi) reduction rate of 1.98 mg/l/h. the cr(vi) reduction rate decreased with increase in cr(vi) concentration from 100 to 500 mg/l. the addition of anthraquinone-2-sulphonic acid (aqs) to culture o. intermedium bcr400 significantly enhanced its chromium reduction rate. the activation energy of aqs ... | 2011 | 22582159 |
| acquired genetic mechanisms of a multiresistant bacterium isolated from a treatment plant receiving wastewater from antibiotic production. | the external environment, particularly wastewater treatment plants (wwtps), where environmental bacteria meet human commensals and pathogens in large numbers, has been highlighted as a potential breeding ground for antibiotic resistance. we have isolated the extensively drug-resistant ochrobactrum intermedium ccug 57381 from an indian wwtp receiving industrial wastewater from pharmaceutical production contaminated with high levels of quinolones. antibiotic susceptibility testing against 47 antib ... | 2013 | 24038701 |
| new extracellular thermostable oxalate oxidase produced from endophytic ochrobactrum intermedium cl6: purification and biochemical characterization. | oxalate oxidase (ec 1.2.3.4) catalyzes the oxidative cleavage of oxalate to carbon dioxide with the reduction of molecular oxygen to hydrogen peroxide. oxalate oxidase found its application in clinical assay for oxalate in blood and urine. this study describes the purification and biochemical characterization of an oxalate oxidase produced from an endophytic bacterium, ochrobactrum intermedium cl6. the cell-free fermentation broth was subjected to two-step enzyme purification, which resulted in ... | 2016 | 26796139 |
| production of eps under cr(vi) challenge in two indigenous bacteria isolated from a tannery effluent. | indigenous cr(vi) reducing bacterial strains pseudomonas aeruginosa rb-1 and ochrobactrum intermedium rb-2 were evaluated for eps production under cr(vi) challenged and free conditions. strain rb-2 was more efficient in total eps production (13.63 mg g(-1)) than rb-1 (4.15 mg g(-1)) under cr(vi) stress. thick covering of capsular material around the cells of both bacterial strains was detected by electron microscopy. transmission electron micrographs showed the appearance of pilli like structure ... | 2015 | 25871833 |
| arachis hypogaea pgpr isolated from argentine soil modifies its lipids components in response to temperature and salinity. | the aim of this work was to clarify the mechanism related to plant growth promoting of a bacterial strain (l115) isolated from arachis hypogaea rhizospheres and the effects of high growth temperature and salinity on phospholipids and fatty acids composition. l115 was isolated from peanut rhizospheres and identified according to the sequence analysis of the 16s rrna gene. phenotypic, metabolic and plant growth promoting rhizobacteria (pgpr) characteristics of l115 were tested. inoculation test in ... | 2015 | 25801965 |
| impact of environmental stress on biochemical parameters of bacteria reducing chromium. | chromium pollution is produced in connection with industrial processes like in tanneries. it has been suggested that bioremediation could be a good option for clean up. the stress effect of variable chromate levels, phs and growth temperatures on biochemical parameters of two cr(vi) reducing bacterial strains pseudomonas aeruginosa rb-1 and ochrobactrum intermedium rb-2 was investigated. transmission electrone microscopy (tem) was performed to study the intracellular distribution of cr(vi). it w ... | 2014 | 25242944 |
| denitrification of industrial wastewater: influence of glycerol addition on metabolic activity and community shifts in a microbial consortium. | the wastewater originating from explosives manufacturing plants are characterized by a high concentration of nitrates (3200mgnl(-1)), sulfates (1470mgl(-1)) and low ph (1.5) as well as the presence of organic compounds, such as nitroglycerin (1.9mgl(-1)) and nitroglycol (4.8mgl(-1)). the application of glycerol (c/n=3) at such a high concentration enabled complete removal of nitrates and did not cause the anaerobic glycerol metabolic pathway of the dnc4 consortium to activate, as confirmed by th ... | 2013 | 24161581 |
| hexavalent chromium reduction by bacteria from tannery effluent. | chromium is generated from several industrial processes. it occurs in different oxidation states, but cr(iii) and cr(vi) are the most common ones. cr(vi) is a toxic, soluble environmental contaminant. some bacteria are able to reduce hexavalent chromium to the insoluble and less toxic cr(iii), and thus chromate bioremediation is of considerable interest. an indigenous chromium-reducing bacterial strain, rb-2, isolated from a tannery water sample, was identified as ochrobactrum intermedium, on th ... | 2012 | 22534304 |
| microbial degradation of n-hexadecane in mineral salt medium as mediated by degradative enzymes. | in the present study, n-hexadecane degradation in msm was investigated by three bacteria identified as pseudomonas aeruginosa psa5, rhodococcus sp. nj2 and ochrobactrum intermedium p2, isolated from petroleum sludge. during 10 days of incubation, n-hexadecane was degraded to 99% by p. aeruginosa psa5, 95% by rhodococcus sp. nj2 and 92% by o. intermedium p2. during degradation process, the induction of catabolic enzymes alkane hydroxylase, alcohol dehydrogenase and lipase were also examined. amon ... | 2012 | 22405754 |
| peeping into genomic architecture by re-sequencing of ochrobactrum intermedium m86 strain during laboratory adapted conditions. | advances in de novo sequencing technologies allow us to track deeper insights into microbial genomes for restructuring events during the course of their evolution inside and outside the host. bacterial species belonging to ochrobactrum genus are being reported as emerging, and opportunistic pathogens in this technology driven era probably due to insertion and deletion of genes. the ochrobactrum intermedium m86 was isolated in 2005 from a case of non-ulcer dyspeptic human stomach followed by its ... | 2016 | 27222803 |
| [a case of bacteremia caused by ochrobacterium intermedium]. | we report herein on a case of bacteremia caused by ochrobactrum intermedium (o. intermedium) identified with biotyper matrix-assisted laser desorption ionization-time of flight mass spectrometry (maldi-tof ms). an 86-year-old man was admitted to our hospital with paralysis of the right side of the body and dysphagia. he was diagnosed as having a pontine infarction based on the brain mri findings and was admitted to hospital to have anti-platelet therapy. three days after admission, he had a feve ... | 2016 | 27197440 |
| draft genome of ochrobactrum intermedium strain m86 isolated from non-ulcer dyspeptic individual from india. | ochrobactrum intermedium is an emerging opportunistic pathogen of humans that is closely related to members of the genus brucella. earlier, we reported the case of an indian subject with non-ulcer dyspeptic symptoms whose urease positive gastric biopsy revealed the presence of helicobacter pylori along with non-helicobacter like bacteria, eventually cultured and identified as o. intermedium strain m86. | 2013 | 23557353 |
| genome sequencing analysis reveals virulence-related gene content of ochrobactrum intermedium strain 229e, a urease-positive strain isolated from the human gastric niche. | we report draft genome sequence of ochrobactrum intermedium strain 229e concurrent with helicobacter pylori in urease positive gastric biopsy of non-ulcer dyspeptic individual from southern part of india. since the role of ochrobactrum in human gastric environment is poorly understood, comprehensive pathological, microbiological, and genome level understanding are necessary to evaluate its association with h. pylori in the gastric niche. comparative analysis of o. intermedium 299e strain reveale ... | 2014 | 25312622 |