| sugarcane yellow leaf virus: an emerging virus that has evolved by recombination between luteoviral and poleroviral ancestors. | we have derived the genomic nucleotide sequence of an emerging virus, the sugarcane yellow leaf virus (scylv), and shown that it produces one to two subgenomic rnas. the family luteoviridae currently includes the luteovirus, polerovirus, and enamovirus genera. with the new scylv nucleotide sequence and existing luteoviridae sequence information, we have utilized new phylogenetic and evolutionary methodologies to identify homologous regions of luteoviridae genomes, which have statistically signif ... | 2000 | 10725208 |
| sugarcane yellow leaf virus: a novel member of the luteoviridae that probably arose by inter-species recombination. | the 5895 nucleotide long single-stranded rna genome of sugarcane yellow leaf virus florida isolate (scylv-f) includes six major orfs. all but the first of these are homologous to genes of known function encoded by viruses of the three newly defined genera in the luteoviridae: ('luteovirids'), i.e. poleroviruses, luccccteoviruses and the enamoviruses. scylv-f orfs 1 and 2 are most closely related to their polerovirus counterparts, whereas scylv-f orfs 3 and 4 are most closely related to counterpa ... | 2000 | 10859394 |
| molecular evidence that sugarcane yellow leaf virus (scylv) is a member of the luteoviridae family. | a previously uncharacterized virus was reported in southeast brazil causing a yellowing leaf disease in sugarcane. the virus, termed sugarcane yellow leaf virus (scylv), shares features typical of the luteoviruses. to start the molecular characterization of scylv, the nucleotide sequence of the coat protein (cp), 17 kda protein and c-terminus of the rna-dependent rna polymerase coding regions was determined from an rt-pcr amplification product. comparisons showed that the deduced amino acid sequ ... | 2000 | 10881686 |
| analyses of genotypic diversity among north, south, and central american isolates of sugarcane yellow leaf virus: evidence for colombian origins and for intraspecific spatial phylogenetic variation. | we have analyzed the genotypic diversity of sugarcane yellow leaf virus (scylv) collected from north, south, and central america by fingerprinting assays and selective cdna cloning and sequencing. one group of isolates from colombia, designated the c-population, has been identified as residing at the root node between a separable superpopulation structure of scylv and other members of the family luteoviridae, indicating that the progenitor viruses of the north, south, and central american isolat ... | 2002 | 11773408 |
| improved detection of sugarcane yellow leaf virus using a real-time fluorescent (taqman) rt-pcr assay. | yellow leaf syndrome (yls) of sugarcane has been associated with sugarcane yellow leaf virus (scylv) and has been reported from most sugarcane growing countries around the world. as sugarcane is vegetatively propagated, it is important to use effective and sensitive detection methods to screen new propagating material. virus detection in symptomatic tissue is currently achieved using enzyme linked immunosorbent assay (elisa), tissue blot immunoassay (tbia) or a conventional rt-pcr based assay. t ... | 2002 | 12008005 |
| a loop 2 cytidine-stem 1 minor groove interaction as a positive determinant for pseudoknot-stimulated -1 ribosomal frameshifting. | the molecular determinants of stimulation of -1 programmed ribosomal frameshifting (-1 prf) by rna pseudoknots are poorly understood. sugarcane yellow leaf virus (scylv) encodes a 28-nt mrna pseudoknot that promotes -1 prf between the p1 (protease) and p2 (polymerase) genes in plant luteoviruses. the solution structure of the scylv pseudoknot reveals a well ordered loop 2 (l2) that exhibits continuous stacking of a20 through c27 in the minor groove of the upper stem 1 (s1), with c25 flipped out ... | 2005 | 16123125 |
| yellow leaf of sugarcane is caused by at least three different genotypes of sugarcane yellow leaf virus, one of which predominates on the island of réunion. | the genetic diversity of sugarcane yellow leaf virus (scylv) was analyzed with 43 virus isolates from réunion island and 17 isolates from world-wide locations. we attempted to amplify by reverse-transcription polymerase chain reaction (rt-pcr), clone, and sequence four different fragments covering 72% of the genome of these virus isolates. the number of amplified isolates and useful sequence information varied according to each fragment, whereas an amplicon was obtained with diagnostic primers f ... | 2006 | 16453082 |
| isolation of nucleotide binding site-leucine rich repeat and kinase resistance gene analogues from sugarcane (saccharum spp.). | resistance gene analogues (rgas) have been isolated from many crops and offer potential in breeding for disease resistance through marker-assisted selection, either as closely linked or as perfect markers. many r-gene sequences contain kinase domains, and indeed kinase genes have been reported as being proximal to r-genes, making kinase analogues an additionally promising target. the first step towards utilizing rgas as markers for disease resistance is isolation and characterization of the sequ ... | 2008 | 17935262 |
| identification of three genotypes of sugarcane yellow leaf virus causing yellow leaf disease from india and their molecular characterization. | sugarcane yellow leaf virus (scylv) that causes yellow leaf disease (yld) in sugarcane (recently reported in india) belongs to polerovirus. detailed studies were conducted to characterize the virus based on partial open reading frames (orfs) 1 and 2 and complete orfs 3 and 4 sequences in their genome. reverse-transcriptase polymerase chain reaction (rt-pcr) was performed on 48 sugarcane leaf samples to detect the virus using a specific set of primers. of the 48 samples, 36 samples (field samples ... | 2008 | 18751882 |
| transmission, characterization, and serology of a luteovirus associated with yellow leaf syndrome of sugarcane. | abstract a previously uncharacterized luteovirus was associated with one form of yellow leaf syndrome (yls), a widespread disease of sugarcane (saccharum sp.). the virus was named sugarcane yellow leaf luteovirus (scylv), and was identified in major sugarcane-producing areas of the world. typical disease symptoms were reproduced when scylv was transmitted by melanaphis sacchari or rhopalosiphum maidis from infected to healthy sugarcane, suggesting that this virus may be the causal agent of one f ... | 2000 | 18944599 |
| the p0 gene of sugarcane yellow leaf virus encodes an rna silencing suppressor with unique activities. | the sugarcane yellow leaf virus (scylv) p0, a member of the highly heterologous proteins of poleroviruses, is a suppressor of posttranscriptional gene silencing (ptgs) and has additional activities not seen in other p0 proteins. the p0 protein in previously tested poleroviruses (beet western yellows virus and cucurbit aphid-borne yellows virus), suppresses local, but not systemic, ptgs induced by both sense gfp and inverted repeat gf using its f-box-like domain to mediate destabilization of the ... | 2009 | 19046592 |
| simultaneous detection and identification of four sugarcane viruses by one-step rt-pcr. | sugarcane mosaic disease (smd) caused by the sugarcane mosaic virus (scmv), sorghum mosaic virus (srmv) and sugarcane streak mosaic virus (scsmv) and sugarcane yellow leaf disease (syld) caused by the sugarcane yellow leaf virus (scylv) are the two most prevalent and economically important viral diseases of sugarcane. in this study, a one-step quadruplex reverse transcription (rt)-pcr method that employed virus-specific primers was developed for the simultaneous detection and differentiation of ... | 2009 | 19646484 |
| detecting sugarcane yellow leaf virus infection in asymptomatic leaves with hyperspectral remote sensing and associated leaf pigment changes. | sugarcane infected with sugarcane yellow leaf virus (scylv) rarely produces visual symptoms until late in the growing season. high-resolution, hyperspectral reflectance data from scylv-infected and non-infected leaves of two cultivars, lcp 85-384 and ho 95-988, were measured and analyzed on 13 july, 12 october, and 4 november 2005. all plants were asymptomatic. infection was determined by reverse transcriptase-polymerase chain reaction (rt-pcr) analysis. results from discriminant analysis showed ... | 2010 | 20362003 |
| a near-complete genome sequence of a distinct isolate of sugarcane yellow leaf virus from china, representing a sixth new genotype. | the 5803 nt genomic sequence of a sugarcane yellow leaf virus (scylv) isolate (scylv-chn1) from china was determined. it covered more than 98% of the complete viral genome and contained all the six orfs and the entire intergenic untranslated region. this isolate was most closely related to scylv genotype cub (isolates cub-yl1 and cb86010) with identities of 95.2-97.4% (nt) (93.2-97.2% aa) in orf0, orf1, and orf2. sequence comparison and phylogenetic analyses supported the view that this isolate ... | 2010 | 20563635 |
| genetic transformation with untranslatable coat protein gene of sugarcane yellow leaf virus reduces virus titers in sugarcane. | sugarcane yellow leaf syndrome, characterized by a yellowing of the leaf midrib followed by leaf necrosis and growth suppression, is caused by sugarcane yellow leaf virus (scylv). we produced scylv-resistant transgenic sugarcane from a susceptible cultivar (h62-4671) and determined the amount of virus present following inoculation. the transgenic plants were produced through biolistic bombardment of cell cultures with an untranslatable coat protein gene. presence of the transgene in regenerated ... | 2011 | 20661641 |
| categorizing sugarcane cultivar resistance to the sugarcane aphid and yellow sugarcane aphid (hemiptera: aphididae). | sugarcane (saccharum spp.) in louisiana is colonized by two aphid species, the sugarcane aphid, melanaphis sacchari (zehntner), and the yellow sugarcane aphid, sipha flava (forbes) (hemiptera: aphididae). the main problem associated with m. sacchari is transmission of sugarcane yellow leaf virus, a casual agent of yellow leaf disease whose absence has been added to certification standards for micropropagated sugarcane in louisiana. greenhouse studies were conducted to categorize dominant commerc ... | 2010 | 20857758 |
| quantification of sugarcane yellow leaf virus in sugarcane following transmission through aphid vector, melanaphis sacchari. | yellow leaf caused by sugarcane yellow leaf virus (scylv) is a serious constraint to sugarcane production in india and currently the disease epidemics occur on many of the susceptible varieties under field conditions. studies were conducted on the virus transmission by sugarcane aphid melanaphis sacchari in sugarcane by inoculating virus-free meristem derived from micro- propagated plants of sugarcane cv co 86032 with viruliferous aphids. virus transmission was confirmed through rt-pcr assays an ... | 2015 | 26645033 |
| biology and management of sugarcane yellow leaf virus: an historical overview. | sugarcane yellow leaf virus (scylv) is one of the most widespread viruses causing disease in sugarcane worldwide. the virus has been responsible for drastic economic losses in most sugarcane-growing regions and remains a major concern for sugarcane breeders. infection with scylv results in intense yellowing of the midrib, which extends to the leaf blade, followed by tissue necrosis from the leaf tip towards the leaf base. such symptomatic leaves are usually characterized by increased respiration ... | 2015 | 26424197 |
| molecular evolutionary history of sugarcane yellow leaf virus based on sequence analysis of rna-dependent rna polymerase and putative aphid transmission factor-coding genes. | rna-dependent rna polymerase (rdrp) encoded by orf2 and putative aphid transmission factor (patf) encoded by orf5 of sugarcane yellow leaf virus (scylv) were detected in six sugarcane cultivars affected by yellow leaf using rt-pcr and real-time rt-pcr assays. expression of both genes varied among infected plants, but overall expression of rdrp was higher than expression of patf. cultivar h87-4094 from hawaii yielded the highest transcript levels of rdrp, whereas cultivar c1051-73 from cuba exhib ... | 2014 | 24952671 |
| prospecting sugarcane resistance to sugarcane yellow leaf virus by genome-wide association. | using gwas approaches, we detected independent resistant markers in sugarcane towards a vectored virus disease. based on comparative genomics, several candidate genes potentially involved in virus/aphid/plant interactions were pinpointed. yellow leaf of sugarcane is an emerging viral disease whose causal agent is a polerovirus, the sugarcane yellow leaf virus (scylv) transmitted by aphids. to identify quantitative trait loci controlling resistance to yellow leaf which are of direct relevance for ... | 2014 | 24916990 |
| genome characterization of sugarcane yellow leaf virus from china reveals a novel recombinant genotype. | sugarcane yellow leaf virus (scylv; genus polerovirus, family luteoviridae) is a recombinant virus associated with yellow leaf disease, a serious threat to sugarcane in china and worldwide. among the nine known scylv genotypes existing worldwide, col, haw, reu, ind, chn1, chn2, bra, cub and per, the last five have been reported in china. in this study, the complete genome sequences (5,880 nt) of gz-gz18 and hn-cp502 isolates from the chinese provinces of guizhou and hainan, respectively, were cl ... | 2014 | 24395076 |
| molecular characterization and phylogenetic analysis of sugarcane yellow leaf virus isolates from china. | sugarcane yellow leaf virus (scylv) (genus polerovirus, family luteoviridae), the causal agent of sugarcane yellow leaf disease (yld), was first detected in china in 2006. to assess the distribution of scylv in the major sugarcane-growing chinese provinces, leaf samples from 22 sugarcane clones (saccharum spp. hybrid) showing yld symptoms were collected and analyzed for infection by the virus using reverse transcription pcr (rt-pcr), quantitative rt-pcr, and immunological assays. a complete geno ... | 2012 | 22752564 |
| putative recombination signature and significance of insertion/deletion events in the rna-dependent rna polymerase coding region of sugarcane yellow leaf virus. | the 5898 nucleotide single-strand rna genome of sugarcane yellow leaf virus (scylv) contains one long open reading frame, which is translated into a 120.6 kda polyprotein. the sequences of scylv isolates from the two scylv-susceptible cultivars from hawaii had a deletion of 48-51 nt in orf1. scylv from 12 sugarcane hybrid cultivars from different origins were tested by rt-pcr using a specific set of primers, to investigate the genome segment for this deletion. only three cultivars were found not ... | 2012 | 22542996 |
| the global structures of a wild-type and poorly functional plant luteoviral mrna pseudoknot are essentially identical. | the helical junction region of a -1 frameshift stimulating hairpin-type mrna pseudoknot from sugarcane yellow leaf virus (scylv) is characterized by a novel c27.(g7-c14) loop 2-stem 1 minor groove base triple, which is stacked on a c8+.(g12-c28) loop 1-stem 2 major groove base triple. substitution of c27 with adenosine reduces frameshifting efficiency to a level just twofold above the slip-site alone. here, we show that the global structure of the c27a scylv rna is nearly indistinguishable from ... | 2006 | 17000902 |
| pairwise coupling analysis of helical junction hydrogen bonding interactions in luteoviral rna pseudoknots. | a 28-nucleotide mrna pseudoknot that overlaps the p1 and p2 genes of sugarcane yellow leaf virus (scylv) stimulates -1 ribosomal frameshifting. the in vitro frameshifting efficiency is decreased >or=8-fold upon substitution of the 3'-most loop 2 nucleotide (c27) with adenosine, which accepts a hydrogen bond from the 2'-oh group of c14 in stem s1. the solution structures of the wild-type (wt) and c27a scylv rna pseudoknots show that while the rnas adopt virtually identical overall structures, the ... | 2006 | 16964977 |