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dyadobacter fermentans gen. nov., sp. nov., a novel gram-negative bacterium isolated from surface-sterilized zea mays stems.a gram-negative bacterium, designated ns114t, was isolated from duplicate treatments of surface-sterilized zea mays stems. the plants were grown in synthetic soil under greenhouse conditions and watered with fertilizer containing no nitrogen. strain ns114t could not be isolated from plants watered with the standard level or 20% (w/v) of the standard level of nitrogen. cells occurred as pairs in young cultures that attached to form angled arrangements in r2a broth and occasionally formed rounded, ...200010758885
isolation and taxonomic affiliation of n-heterocyclic aromatic hydrocarbon-transforming bacteria.the azaarenes (nitrogen-containing heterocyclic aromatic hydrocarbons) are products of incomplete combustion processes and thus are widely distributed with tar and oil products in the environment. despite their adverse organoleptic, toxic, and carcinogenic characteristics, the biodegradability and fate of multi-ring azaarenes have received little attention. this work demonstrates the presence of genetically diverse azaarene-degrading bacteria in coal tar-contaminated soils. thirty-eight bacteria ...200515650851
dyadobacter crusticola sp. nov., from biological soil crusts in the colorado plateau, usa, and an emended description of the genus dyadobacter chelius and triplett 2000.bacterial strain cp183-8(t) was isolated from biological soil crusts collected in the colorado plateau, usa. cells of this strain were aerobic, non-motile, gram-negative, psychrotolerant and formed beaded chains in the stationary growth phase. they contained c(16 : 1)omega5c and c(16 : 1)omega7c as major fatty acids. 16s rrna gene sequence analysis assigned the strain to the genus dyadobacter. however, it shared a sequence similarity of only 95.88 % with the type strain of dyadobacter fermentans ...200515879270
dyadobacter hamtensis sp. nov., from hamta glacier, located in the himalayas, india.strain hhs 11(t) was isolated from a water sample collected from the snout of hamta glacier located in the himalayan mountain ranges of india. phenotypic, chemotaxonomic and phylogenetic analyses established the affiliation of the isolate to the genus dyadobacter. hhs 11(t) possessed 96 and 95% 16s rrna gene sequence similarity with respect to dyadobacter crusticola and dyadobacter fermentans, respectively. furthermore, strain hhs 11(t) differs from d. crusticola and d. fermentans in a number of ...200516166718
dyadobacter ginsengisoli sp. nov., isolated from soil of a ginseng field.a gram-negative, aerobic, non-motile, non-spore-forming and rod-shaped bacterium, strain gsoil 043(t), was isolated from soil from a ginseng field in pocheon province, south korea. the novel isolate was characterized in order to determine its taxonomic position. on the basis of 16s rrna gene sequence similarity, strain gsoil 043(t) was shown to belong to the family 'flexibacteraceae' and was related to dyadobacter fermentans (96.7 %), dyadobacter crusticola (96.3 %) and dyadobacter hamtensis (95 ...200616902034
dyadobacter beijingensis sp. nov., isolated from the rhizosphere of turf grasses in china.strain a54(t) was isolated from rhizospheric soil of turf grasses irrigated with reclaimed water in taoranting park, beijing, china. phenotypic, chemotaxonomic and polygenetic analyses established the affiliation of the isolate to the genus dyadobacter. strain a54(t) possessed 97.7, 94.4 and 94.7 % 16s rrna gene sequence similarity with respect to the type strains of dyadobacter fermentans, d. hamtensis and d. crusticola, respectively. furthermore, dna-dna hybridization did not show significant ...200717392220
establishing a risk-assessment process for release of genetically modified wine yeast into the environment.the use and release of genetically modified organisms (gmos) is an issue of intense public concern and, in the case of food and beverages, products containing gmos or products thereof carry the risk of consumer rejection. the recent commercialization of 2 gm wine yeasts in the united states and canada has made research and development of risk assessments for gm microorganisms a priority. the purpose of this study was to take a first step in establishing a risk-assessment process for future use a ...200919898539
dyadobacter soli sp. nov., a starch-degrading bacterium isolated from farm soil.a gram-negative, non-motile, aerobic bacterial strain, designated mj20(t), was isolated from farm soil near daejeon (south korea) and was characterized taxonomically by using a polyphasic approach. comparative 16s rrna gene sequence analysis showed that strain mj20(t) belongs to the family cytophagaceae, class sphingobacteria, and was related most closely to dyadobacter fermentans dsm 18053(t) (98.9 % sequence similarity), dyadobacter beijingensis jcm 14200(t) (98.0 %) and dyadobacter ginsengiso ...201020008109
complete genome sequence of dyadobacter fermentans type strain (ns114).dyadobacter fermentans (chelius and triplett, 2000) is the type species of the genus dyadobacter. it is of phylogenetic interest because of its location in the cytophagaceae, a very diverse family within the order 'sphingobacteriales'. d. fermentans has a mainly respiratory metabolism, stains gram-negative, is non-motile and oxidase and catalase positive. it is characterized by the production of cell filaments in aging cultures, a flexirubin-like pigment and its ability to ferment glucose, which ...200921304649
dyadobacter endophyticus sp. nov., an endophytic bacterium isolated from maize root.a novel gram-stain-negative, aerobic and rod-shaped bacterial strain, designated 65t, was isolated from surface-sterilized root tissue of maize, collected from fangshan district of beijing, people's republic of china, and was subjected to a taxonomic study by using a polyphasic approach. phylogenetic analysis based on 16s rrna gene sequences showed that strain 65t belonged to the genus dyadobacter and had highest 16s rrna gene sequence similarity to dyadobacter jiangsuensis cgmcc 1.12969t (99.1 ...201627412002
an inverting β-1,2-mannosidase belonging to glycoside hydrolase family 130 from dyadobacter fermentans.the glycoside hydrolase family (gh) 130 is composed of inverting phosphorylases that catalyze reversible phosphorolysis of β-d-mannosides. here we report a glycoside hydrolase as a new member of gh130. dfer_3176 from dyadobacter fermentans showed no synthetic activity using α-d-mannose 1-phosphate but it released α-d-mannose from β-1,2-mannooligosaccharides with an inversion of the anomeric configuration, indicating that dfer_3176 is a β-1,2-mannosidase. mutational analysis indicated that two gl ...201526476324
dyadobacter jiangsuensis sp. nov., a methyl red degrading bacterium isolated from a dye-manufacturing factory.a gram-stain-negative, non-motile, rod-shaped bacterial strain, l-1(t), which was capable of degrading methyl red was isolated from a dye-manufacturing factory in china. phenotypic, chemotaxonomic and phylogenetic analyses established affiliation of the isolate to the genus dyadobacter . cells occurred in pairs in young cultures but became chains of coccoid cells in old cultures, and produced a flexirubin-like yellow pigment. strain l-1(t) could not hydrolyse cellulose, and had a dna g+c content ...201525604339
identification and characterization of cellobiose 2-epimerases from various aerobes.cellobiose 2-epimerase (ce), found mainly in anaerobes, reversibly converts d-glucose residues at the reducing end of β-1,4-linked oligosaccharides to d-mannose residues. in this study, we characterized ce-like proteins from various aerobes (flavobacterium johnsoniae nbrc 14942, pedobacter heparinus nbrc 12017, dyadobacter fermentans atcc 700827, herpetosiphon aurantiacus atcc 23779, saccharophagus degradans atcc 43961, spirosoma linguale atcc 33905, and teredinibacter turnerae atcc 39867), beca ...201323291764
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