| the role of tandem acyl carrier protein domains in polyunsaturated fatty acid biosynthesis. | acyl carrier protein (acp) plays an essential role in fatty acid and polyketide biosynthesis, and most of the fatty acid synthases (fass) and polyketide synthases (pkss) known to date are characterized with a single acp for each cycle of chain elongation. polyunsaturated fatty acid (pufa) biosynthesis is catalyzed by the pufa synthase, and all pufa synthases known to date contain tandem acps (ranging from 5 to 9). using the pfa pufa synthase from shewanella japonica as a model system, we report ... | 2008 | 18444614 |
| complete genome of a metabolically-diverse marine bacterium shewanella japonica kctc 22435(t). | shewanella japonica kctc 22435(t) is a facultatively anaerobic, gram-negative, mesophilic, rod-shaped bacterium isolated from sea water at the pacific institute of bio-organic chemistry of the marine experimental station, troitza bay, gulf of peter the great, russia. here, we report the complete genome of s. japonica kctc 22435(t), which consists of 4,975,677bp (g+c content of 40.80%) with a single chromosome, 4036 protein-coding genes, 97 trnas and 8 rrna operons. genes detected in the genome r ... | 2017 | 28522160 |
| shewanella intestini sp. nov., isolated from the intestine of abalone, haliotis diversicolor. | a gram-stain-negative, rod-shaped bacterium with a singular polar flagellum, designated as strain xmddzsb0408t, was isolated from the intestine of adult abalone, haliotis diversicolor. activity of oxidase was positive and catalase activity was negative. it could grow at salinities from 3 to 6 % nacl (w/v), and ph 7-9. it had the highest sequence similarity of <96.0 % with all previously established species for the complete 16s rrna gene (1531 bp). the results of phylogenetic analysis indicated t ... | 2017 | 28629494 |
| effect of intestinal autochthonous probiotics isolated from the gut of sea cucumber (apostichopus japonicus) on immune response and growth of a. japonicus. | the study isolated 224 bacteria from the intestine of apostichopus japonicus, then selected and identified three of the bacteria (hs1, hs7, and hs10) which demonstrated amylase, lipase, and protease production capacity as candidate probiotics for sea cucumbers. the three potential probiotics showed no pathogenicity both in hemolytic assays on sheep blood agar plates and after immersing sea cucumbers in a suspension of the bacteria. to reveal the effects of these three potential probiotics on the ... | 2014 | 24727198 |
| Structure of the O-Specific polysaccharide from Shewanella japonica KMM 3601 containing 5,7-Diacetamido-3,5,7,9-tetradeoxy-D-glycero-D-talo-non-2-ulosonic acid. | Structure of the O-specific polysaccharide chain of the lipopolysaccharide (LPS) of Shewanella japonica KMM 3601 was elucidated. The initial and O-deacylated LPS as well as a trisaccharide representing the O-deacetylated repeating unit of the O-specific polysaccharide were studied by sugar analysis along with 1H and 13C NMR spectroscopy. The polysaccharide was found to contain a rare higher sugar, 5,7-diacetamido-3,5,7,9-tetradeoxy-D-glycero-D-talo-non-2-ulosonic acid (a derivative of 4-epilegio ... | 2011 | 21999540 |
| the utility of shewanella japonica for microbial fuel cells. | shewanella-containing microbial fuel cells (mfcs) typically use the fresh water wild-type strain shewanella oneidensis mr-1 due to its metabolic diversity and facultative oxidant tolerance. however, s. oneidensis mr-1 is not capable of metabolizing polysaccharides for extracellular electron transfer. the applicability of shewanella japonica (an agar-lytic shewanella strain) for power applications was analyzed using a diverse array of carbon sources for current generation from mfcs, cellular phys ... | 2011 | 20663660 |
| tandem acyl carrier protein domains in polyunsaturated fatty acid synthases. | polyunsaturated fatty acids (pufas) can be biosynthesized via aerobic pathways that rely on combinations of desaturases and elongases to convert saturated fatty acids to pufas or anaerobic pathways that exploit polyketide synthase (pks)-like enzymes known as pufa synthases for de novo synthesis from acyl coa precursors. in contrast to most fatty acid synthases (fass) and pkss that contain a single acyl carrier protein (acp) domain for each cycle of fatty acid or polyketide chain elongation, all ... | 2009 | 19362636 |
| shewanella donghaensis sp. nov., a psychrophilic, piezosensitive bacterium producing high levels of polyunsaturated fatty acid, isolated from deep-sea sediments. | a gram-negative, motile, rod-shaped, psychrophilic bacterium, lt17t, was isolated from deep-sea sediments (3300 m depth) of the east sea (sea of japan). optimal growth of lt17t requires the presence of 2.5 % (w/v) nacl, a ph of 7.0-7.5 and a temperature of 17 degrees c. the isolate grows optimally under a hydrostatic pressure of 10 mpa and growth is possible between 0.1 and <30 mpa. the novel strain is positive in tests for catalase, oxidase, lipase, beta-glucosidase and gelatinase activities an ... | 2007 | 17267951 |
| enhanced biofilm formation and increased resistance to antimicrobial agents and bacterial invasion are caused by synergistic interactions in multispecies biofilms. | most biofilms in their natural environments are likely to consist of consortia of species that influence each other in synergistic and antagonistic manners. however, few reports specifically address interactions within multispecies biofilms. in this study, 17 epiphytic bacterial strains, isolated from the surface of the marine alga ulva australis, were screened for synergistic interactions within biofilms when present together in different combinations. four isolates, microbacterium phyllosphaer ... | 2006 | 16751497 |
| structure of the o-specific polysaccharide from shewanella japonica type strain kmm 3299t containing the rare amino sugar fuc4nac. | an acidic o-specific polysaccharide (ps) of the agar-digesting bacterium shewanella japonica with the type strain kmm 3299(t) was obtained by mild acid hydrolysis of the lipopolysaccharide. the polysaccharide was studied by component analysis, methylation analysis, (1)h and (13)c nmr spectroscopy, including 2d nmr experiments. the ps was determined to have the following structure involving three unusual amino sugars: | 2005 | 15882857 |
| shewanella pacifica sp. nov., a polyunsaturated fatty acid-producing bacterium isolated from sea water. | six marine bacterial strains, kmm 3597t, kmm 3775, kmm 3590, kmm 3772, kmm 3605 and kmm 3601, that produce polyunsaturated fatty acids were isolated from sea water samples collected from different locations and depths in chazhma bay (sea of japan, pacific ocean) and characterized to clarify their taxonomic position. the dna g+c contents of these strains were 39.5-40.3 mol%. the level of dna hybridization between these strains was conspecific (83-96%), indicating that they represent a single geno ... | 2004 | 15280273 |
| shewanella olleyana sp. nov., a marine species isolated from a temperate estuary which produces high levels of polyunsaturated fatty acids. | two polyunsaturated fatty acid (pufa) producing strains (acem 6 and acem 9(t)) isolated from a temperate, humic-rich river estuary in tasmania, australia, were found to be members of the genus shewanella. these strains were able to utilize humic compounds (tannic acid) and derivatives (2,6-anthraquinone disulfonate) as sole carbon sources and as electron acceptors for anaerobic respiration. the major fatty acids were typical of the genus shewanella; however, pufas mostly made up of eicosapentaen ... | 2002 | 12508875 |
| shewanella japonica sp. nov. | two strains of agar-digesting bacteria, kmm 3299t and kmm 3300, respectively isolated from sea water and the mussel protothaca jedoensis, have been characterized. based on sequencing of the 16s rrna gene, kmm 3299t showed the highest similarity (93-95%) to members of the genus shewanella. the g+c contents of the dnas of these strains were 43-44 mol%. the level of dna homology between the two strains was conspecific (95%), indicating that they represent a distinct genospecies. these organisms wer ... | 2001 | 11411670 |