description of pandoraea gen. nov. with pandoraea apista sp. nov., pandoraea pulmonicola sp. nov., pandoraea pnomenusa sp. nov., pandoraea sputorum sp. nov. and pandoraea norimbergensis comb. nov. | a polyphasic taxonomic study was performed on a group of isolates tentatively identified as burkholderia cepacia, ralstonia pickettii or ralstonia paucula (formerly known as cdc group ivc-2). the isolates were mainly cultured from sputum of cystic fibrosis patients or from soil. sds-page of whole-cell proteins and aflp fingerprinting distinguished at least five different species, and this was confirmed by dna-dna hybridizations. 16s rdna sequence analysis of representative strains indicated that ... | 2000 | 10758901 |
identification of pandoraea species by 16s ribosomal dna-based pcr assays. | the recently described genus pandoraea contains five named species (pandoraea apista, pandoraea pulmonicola, pandoraea pnomenusa, pandoraea sputorum, and pandoraea norimbergensis) and four unnamed genomospecies. pandoraea spp. have mainly been recovered from the respiratory tracts of cystic fibrosis (cf) patients. accurate genus- and species-level identification by routine clinical microbiology methods is difficult, and differentiation from burkholderia cepacia complex organisms may be especiall ... | 2001 | 11724860 |
use of amplified ribosomal dna restriction analysis for identification of ralstonia and pandoraea species: interest in determination of the respiratory bacterial flora in patients with cystic fibrosis. | the recovery of ralstonia and pandoraea species from respiratory tract cultures of patients with cystic fibrosis has recently been reported. these species are difficult to identify, and especially to differentiate from burkholderia cepacia complex organisms, with classical methods. the discriminatory power of amplified ribosomal dna restriction analysis (ardra) within the two genera was assessed by comparing the restriction profiles of reference strains of each species by using a panel of six en ... | 2003 | 12843108 |
evaluation of in vitro virulence characteristics of the genus pandoraea in lung epithelial cells. | pandoraea species are emerging opportunistic pathogens capable of causing chronic lung infections in cystic fibrosis patients. this study examined the interactions of 17 pandoraea isolates from the five identified species (pandoraea apista, pandoraea norimbergensis, pandoraea pulmonicula, pandoraea sputorum and pandoraea pnomenusa) plus two pandoraea genomospecies isolates with lung epithelial cells and their ability to form biofilms in vitro. only three isolates showed an ability to invade a549 ... | 2008 | 18065662 |
isolation and characterization of novel bacterial strains exhibiting ligninolytic potential. | to expand on the range of products which can be obtained from lignocellulosic biomass, the lignin component should be utilized as feedstock for value-added chemicals such as substituted aromatics, instead of being incinerated for heat and energy. enzymes could provide an effective means for lignin depolymerization into products of interest. in this study, soil bacteria were isolated by enrichment on kraft lignin and evaluated for their ligninolytic potential as a source of novel enzymes for wast ... | 2011 | 21995752 |