Publications

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effects of dietary forage-to-concentrate ratio on nutrient digestibility and enteric methane production in growing goats (capra hircus hircus) and sika deer (cervus nippon hortulorum).two experiments were conducted to determine the effects of forage-to-concentrate (f:c) ratio on the nutrient digestibility and enteric methane (ch4) emission in growing goats and sika deer. three male growing goats (bw = 19.0 ± 0.7 kg) and three male growing deer (bw = 19.3 ± 1.2 kg) were respectively allotted to a 3 × 3 latin square design with an adaptation period of 7 d and a data collection period of 3 d. respiration-metabolism chambers were used for measuring the enteric ch4 emission. treat ...201728335097
dybowski's sika deer (cervus nippon hortulorum): genetic divergence between natural primorian and introduced czech populations.dybowski's sika deer (cervus nippon hortulorum) originally inhabited the majority of the primorsky krai in far eastern russia, north-eastern china, and korean peninsula. at present, only the russian population seems to be stable, even though this taxon is still classified as endangered by the russian federation. almost 100 years ago, this subspecies, among others, was imported to several european countries including the czech republic. we used both mitochondrial (mtdna; the cytochrome b gene and ...201323454911
the construction of cloned sika deer embryos (cervus nippon hortulorum) by demecolcine auxiliary enucleation.the objective of our study was to establish the feasibility of experimental protocols for cloning sika deer. we performed auxiliary enucleation to improve the efficiency of nuclear transfer operation by optimizing the demecolcine concentration to induce cytoplasmic protrusions in the sika deer oocytes. in the present study,we had studied the impact of different demecolcine concentrations on cytoplasmic protrusions and enucleation rates. we determined that 95.9% of the sika deer oocytes formed cy ...201424138424
complete mitochondrial genome sequence of northeastern sika deer (cervus nippon hortulorum).the complete mitochondrial genome of the northeastern sika deer, cervus nippon hortulorum, was determined by accurate polymerase chain reaction. the entire genome is 16,434 bp in length and contains 13 protein-coding genes, 2 rrna genes, 22 trna genes and 1 control region, all of which are arranged in a typical vertebrate manner. the overall base composition of the northeastern sika deer's mitochondrial genome is 33.3% of a, 24.5% of c, 28.7% of t and 13.5% of g. a termination associated sequenc ...201624660928
serum hormone concentrations and ovarian follicular wave emergence in jilin sika deer (cervus nippon hortulorum) after synchronization of estrous cycles.the study was conducted to investigate the serum hormone concentrations and follicular dynamics present after synchronous treatment (cidr) in female jilin sika deer (n = 15) of estrous cycles. blood samples were collected to analyze the fsh, lh, estradiol and progesterone during the estrous cycles. manual transrectal ultrasonography examination was conducted at least thrice weekly to monitor the follicular wave. ultrasonography showed that follicle development occurred in waves, and most estrous ...201525563157
the complete mitochondrial genome of sika deer cervus nippon hortulorum (artiodactyla: cervidae) and phylogenetic studies.sika deer (cervus nippon temminck 1836) are classified in the order artiodactyla, family cervidae, subfamily cervinae. at present, the phylogenetic studies of c. nippon are problematic. in this study, we first determined and described the complete mitochondrial sequence of the wild c. nippon hortulorum. the complete mitogenome sequence is 16 566 bp in length, including 13 protein-coding genes, two rrna genes, 22 trna genes, a putative control region (cr) and a light-strand replication origin (ol ...201626258510
genetic diversity and population structure of a sichuan sika deer (cervus sichuanicus) population in tiebu nature reserve based on microsatellite variation.cervus sichuanicus is a species of sika deer (cervus nippon group). to date, research has mainly focused on quantity surveying and behavior studies, with genetic information on this species currently deficient. to provide scientific evidence to assist in the protection of this species, we collected sichuan sika deer fecal samples from the sichuan tiebu nature reserve (tnr) and extracted dna from those samples. microsatellite loci of bovine were used for pcr amplification. after genescan, the gen ...201425465089
wild and farm breeding cervids infections with anaplasma phagocytophilum.the main goal of our study was to determine the prevalence of anaplasma phagocytophilum infections in wild cervids living in north-eastern part of poland. material used in the study was gathered between the years 2004- 2008. the blood samples from 106 red deer (cervus elaphus), 32 sika deer (cervus nippon hortulorum), 130 fallow deer (dama dama) and 31 roe deer (capreolus capreolus) were collected. dna was isolated using genomic mini ax blood kit (a and a biotechnology). molecular detection of a ...201121739932
molecular cloning and gene expression analysis of ercc6l in sika deer (cervus nippon hortulorum).one important protein family that functions in nucleotide excision repair (ner) factors is the snf2 family. a newly identified mouse ercc6-like gene, ercc6l (excision repair cross-complementing rodent repair deficiency, complementation group 6-like), has been shown to be another developmentally related member of the snf2 family.201121695076
polymorphisms of the prion protein gene (prnp) in chinese domestic sika deer (cervus nippon hortulorum). 200515932416
application of mitochondrial dna sequence analysis in the forensic identification of chinese sika deer subspecies.as a direct and indirect consequence of human activities, only two subspecies, cervus nippon sinchuanicus and cervus nippon kopschi, currently subsist in the wild of china. however, a large population of cervus nippon hortulorum and cervus nippon nippon is raised in order to gain deer parts for chinese traditional medicine. according to chinese wild animal conservation law, hunting, capturing and trading of the wild sika deer are strictly banned, however, raising and trading of the domestic indi ...200515639603
optimizing the conditions for in vitro maturation and artificial activation of sika deer (cervus nippon hortulorum) oocytes.with the goal of establishing experimental protocols for cloning sika deer, various conditions for in vitro maturation (ivm) and artificial activation of sika deer oocytes were examined. in vitro maturation was evaluated in seven different culture media. the highest rate of oocyte maturation was 75.4% in 10 μg/ml follicle-stimulating hormone (fsh), 1 μg/ml lh, 0.2 mm cysteamine and 50 ng/ml epidermal growth factor (egf) after 24 h of ivm. the efficiency after 24 h of ivm did not differ significa ...201322458270
[analysis of expressed sequence tags (ests) in sika deer (cervus nippon hortulorum) velvet tip tissue].a total of 906 high quality ests were obtained through random sequencing from velvet tip tissue cdna library of sika deer (cervus nippon hortulorum). clustering of 906 ests resulted in a set of 701 unigenes comprising of 86 contigs and 615 singletons. blast analysis revealed that 580 unigenes (82.7% of all 701 unigenes) were matched to the known genes or putative genes in public databases, which could be classified into molecular function, biological process or cellular component according to th ...201121482528
three polymorphic chromosome systems of centric fusion type in a population of manchurian sika deer (cervus nippon hortulorum swinhoe). 19695392210
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