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16s ribosomal dna characterization of nitrogen-fixing bacteria isolated from banana (musa spp.) and pineapple (ananas comosus (l.) merril).nitrogen-fixing bacteria isolated from banana (musa spp.) and pineapple (ananas comosus (l.) merril) were characterized by amplified 16s ribosomal dna restriction analysis and 16s rrna sequence analysis. herbaspirillum seropedicae, herbaspirillum rubrisubalbicans, burkholderia brasilensis, and burkholderia tropicalis were identified. eight other types were placed in close proximity to these genera and other alpha and beta proteobacteria.200111319127
five novel acid-tolerant oligotrophic thiosulfate-metabolizing chemolithotrophic acid mine drainage strains affiliated with the genus burkholderia of betaproteobacteria and identification of two novel soxb gene homologues.five acid-tolerant thiosulfate-metabolizing bacteria were isolated from acid mine drainage samples from garubathan, india. 16s rrna gene analysis revealed that the strains were affiliated with the genus burkholderia of the class of betaproteobacteria. comparative 16s rrna gene sequence analyses indicated that the strains designated as gah1 and gah2 produced a separate phylogenetic branch having burkholderia pyrrocinia atcc 51958(t) (96-98%) as the closest relative. strains gah4 and burkholderia ...201121349327
solution conformation and dynamics of exopolysaccharides from burkholderia species.exopolysaccharides (epss) from the burkholderia genus are proposed to be involved in pathological conditions in humans, such as cystic fibrosis and septicemia, as well as in the stability of soil aggregates. hence, considering that the conformational and dynamic aspects of such epss may influence their biological activity, the current work employs a series of molecular dynamics simulations on di-, oligo-, and polysaccharide fragments of three epss, from burkholderia caribensis, burkholderia cepa ...201020633874
burkholderia heleia sp. nov., a nitrogen-fixing bacterium isolated from an aquatic plant, eleocharis dulcis, that grows in highly acidic swamps in actual acid sulfate soil areas of vietnam.nitrogen-fixing bacteria, strains sa41(t), sa42 and sa53, were isolated from an aquatic plant, eleocharis dulcis, that grows in highly acidic swamps (ph 2-4) in actual acid sulfate soil areas of vietnam. the isolates were gram-negative, aerobic, non-spore-forming, rod-shaped bacteria, having a cell width of 0.6-0.7 microm and a length of 1.5-1.7 microm. they showed good growth between ph 3.0 and 7.0, and between 17 and 37 degrees c. the organisms contained ubiquinone q-8 as the predominant isopr ...201019666801
first isolation of burkholderia tropica from a neonatal patient successfully treated with imipenem.we report the first case of a human burkholderia tropica infection. the patient was a premature neonate who had necrotizing enterocolitis with bowel perforation requiring surgical intervention. the stoma care and difficulties in feeding were a chronic problem. at the age of almost 4 months he developed septicemia due to b. tropica. three consecutive blood cultures grew this organism. the organism was cleared from the blood after a course of imipenem and resolution of post-operative ileus. our ca ...201019482535
burkholderia ferrariae sp. nov., isolated from an iron ore in brazil.a gram-negative, non-spore-forming bacterial strain with the ability to solubilize highly insoluble phosphatic minerals was isolated from a high-phosphorous iron ore from minas gerais state, brazil. this strain, designated fegl01(t), was subjected to a polyphasic taxonomic investigation. comparative 16s rrna gene sequence analysis indicated that it formed a distinct phylogenetic lineage within the genus burkholderia together with several other species of the genus, e.g. burkholderia sacchari, bu ...200617012573
culture conditions for the production of an acidic exopolysaccharide by the nitrogen-fixing bacterium burkholderia tropica.the endophytic diazotrophic bacterium burkholderia tropica, strain ppe8, produced copious amounts of exopolysaccharide (eps) on batch growth in liquid synthetic media containing mannitol and glutamate as carbon and nitrogen sources. the effect of various aeration regimes and carbon source concentrations on eps production was determined, as well as the effects of temperature and time of growth. the degree of aeration had a great influence on the yield of eps, in contrast with the c:n ratio of the ...200616699575
burkholderia tropica sp. nov., a novel nitrogen-fixing, plant-associated bacterium.in an ecological survey of nitrogen-fixing bacteria isolated from the rhizosphere and as endophytes of sugarcane, maize and teosinte plants in brazil, mexico and south africa, a new phylogenetically homogeneous group of n(2)-fixing bacteria was identified within the genus burkholderia. this polyphasic taxonomic study included microscopic and colony morphology, api 20ne tests and growth on different culture media at different ph and temperatures, as well as carbon source assimilation tests and wh ...200415545451
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